ABACUS2 FRET biosensors allow an unparalleled live view of the dynamics of the plant hormone abscisic acid in plants. Well-watered roots accumulate abscisic acid in growing cells when shoots become dehydrated — a response that is essential to maintain root growth in low-humidity conditions.
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References
Jones, A. M. A new look at stress: abscisic acid patterns and dynamics at high‐resolution. New Phytol 210, 38–44 (2016). A review article that describes the transport, metabolism and role of ABA in stress responses.
Jones, A. M. et al. Abscisic acid dynamics in roots detected with genetically encoded FRET sensors. Elife 3, e01741 (2014). This paper reports the first generation ABACUS1 sensors for ABA.
Waadt, R. et al. Dual-reporting transcriptionally linked genetically encoded fluorescent indicators resolve the spatiotemporal coordination of cytosolic abscisic acid and second messenger dynamics in arabidopsis. Plant Cell 32, 2582–2601 (2020). This paper reports the ABAleonSD1-3L21 sensor for ABA.
Mehra, P. et al. Hydraulic flux-responsive hormone redistribution determines root branching. Science. 378, 762–768 (2022). This article uses ABACUS2 to demonstrate hydraulic movement of ABA in roots.
Rizza, A. et al. Differential biosynthesis and cellular permeability explain longitudinal gibberellin gradients in growing roots. Proc. Natl Acad. Sci. 118, e1921960118 (2021). This paper combines modelling and quantitative experiments to establish how root gibberellin patterns are formed.
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This is a summary of: Rowe, J. et al. Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity. Nat. Plants https://doi.org/10.1038/s41477-023-01447-4 (2023).
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Visualising abscisic acid dynamics at low humidity in planta using ABACUS2. Nat. Plants 9, 1016–1017 (2023). https://doi.org/10.1038/s41477-023-01469-y
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DOI: https://doi.org/10.1038/s41477-023-01469-y