A major bottleneck in plant breeding is the establishment or breakage of genetic linkages by random, naturally occurring meiotic recombination. This problem can be overcome by CRISPR–Cas-mediated chromosome engineering. By inverting ~17 Mb of chromosome 2 of Arabidopsis thaliana, we almost completely suppressed genetic crossovers in nearly the entire chromosome.
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Rönspies, M., Dorn, A., Schindele, P. & Puchta, H. CRISPR–Cas-mediated chromosome engineering for crop improvement and synthetic biology. Nat. Plants 7, 566–573 (2021). This review discusses applications of chromosome engineering in synthetic biology and plant breeding.
Schmidt, C., Fransz, P., Rönspies, M., Dreissig, S., Fuchs, J., Heckmann, S., Houben, A. & Puchta, H. Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering. Nat. Commun. 11, 4418 (2020). This paper shows that recombination can be locally enhanced by chromosome engineering.
Beying, B., Schmidt, C., Pacher, M., Houben, A. & Puchta, H. CRISPR–Cas9-mediated induction of heritable chromosomal translocations in. Arabidopsis. Nat. Plants 6, 638–645 (2020). This paper shows that it is possible to segregate linked traits by chromosome engineering.
Rönspies, M., Schindele, P., Wetzel, R. & Puchta, H. CRISPR–Cas9-mediated chromosome engineering in Arabidopsis thaliana. Nat. Protoc. 17, 1332–1358 (2022). This protocol describes the procedure of obtaining rare chromosomal rearrangement events in plants by chromosome engineering.
Schwartz, C. et al. CRISPR–Cas9-mediated 75.5-Mb inversion in maize. Nat. Plants 6, 1427–1431 (2020). This paper shows that heritable inversions can also be achieved in crop plants (maize).
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This is a summary of: Rönspies, M. et al. Massive crossover suppression by CRISPR–Cas-mediated plant chromosome engineering. Nat. Plants https://doi.org/10.1038/s41477-022-01238-3 (2022).
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Redirecting meiotic recombination by CRISPR–Cas-mediated chromosome engineering. Nat. Plants 8, 1144–1145 (2022). https://doi.org/10.1038/s41477-022-01239-2