Abstract
At present, a cooperative process hypothesis is used to explain the supply of enzyme (class III peroxidases and/or laccases) and substrates during lignin polymerization. However, it remains elusive how xylem cells meet the needs of early lignin rapid polymerization during secondary cell wall formation. Here we provide evidence that a mitochondrial ascorbate peroxidase (PtomtAPX) is responsible for autonomous lignification during the earliest stage of secondary cell wall formation in Populus tomentosa. PtomtAPX was relocated to cell walls undergoing programmed cell death and catalysed lignin polymerization in vitro. Aberrant phenotypes were caused by altered PtomtAPX expression levels in P. tomentosa. These results reveal that PtomtAPX is crucial for catalysing lignin polymerization during the early stages of secondary cell wall formation and xylem development, and describe how xylem cells provide autonomous enzymes needed for lignin polymerization during rapid formation of the secondary cell wall by coupling with the programmed cell death process.
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Data availability
The data that support the findings of this study are openly available in Science Data Bank at http://cstr.cn/31253.11.sciencedb.j00001.00446. Sequence data in this article can be found in the GenBank database under accession number PtomtAPX (MG921618.1) at https://www.ncbi.nlm.nih.gov/nuccore/MG921618.1/. Source data are provided with this paper.
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Acknowledgements
This work was supported by the Natural Science Foundation of China (31971618) and National Key Research and Development Program of China (2021YFD2200900).
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H. Lu designed the experiments; J.Z., Y.L. and C.L. performed the experiments; X.L., X.G., C.Z., D.L. and B.Y. performed statistical analysis; H. Lu, J.Z., H. Li and I.H. wrote the manuscript.
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Supplementary Tables 1 and 2.
Supplementary Video 1
Relocation of PtomtAPX-GFP fusion protein in transgenic tobacco suspension cells during PCD.
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Unprocessed western blots and gels for Fig. 1.
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Unprocessed western blots and gels for Fig. 6.
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Zhang, J., Liu, Y., Li, C. et al. PtomtAPX is an autonomous lignification peroxidase during the earliest stage of secondary wall formation in Populus tomentosa Carr. Nat. Plants 8, 828–839 (2022). https://doi.org/10.1038/s41477-022-01181-3
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DOI: https://doi.org/10.1038/s41477-022-01181-3
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