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Shutting the door on polysaccharide methylation

Methylation is a frequent modification of glycans and polysaccharides. This process relies on the transport of cytosolic S-adenosyl-l-methionine (SAM) into the Golgi lumen. This work identifies Golgi-localized major facilitator superfamily transporter members as putative SAM transporters in plants and reveals their importance for polysaccharide methylation and maintenance of the properties of cell walls.

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Fig. 1: Discovery of Golgi SAM transporters reveals the importance of polysaccharide methylation.

References

  1. Ibar, C. & Orellana, A. The import of S-adenosylmethionine into the Golgi apparatus is required for the methylation of homogalacturonan. Plant Physiol. 145, 504–512 (2007). This work shows that SAM incorporation into pectin occurs in Golgi vesicles from plants.

    CAS  Article  Google Scholar 

  2. Nikolovski, N., Shliaha, P. V., Gatto, L., Dupree, P. & Lilley, K. S. Label-free protein quantification for plant Golgi protein localization and abundance. Plant Physiol. 166, 1033–1043 (2014). In this paper, Golgi proteomic experiments identify MFS_5 members as abundant proteins in the organelle.

    Article  Google Scholar 

  3. Wohlschlager, T. et al. Methylated glycans as conserved targets of animal and fungal innate defense. Proc. Natl Acad. Sci. USA 111, E2787–2796 (2014). This work in C. elegans showed that an MFS_5 mutation results in reduced methylation of glycans.

    CAS  Article  Google Scholar 

  4. Jarvis, M. C. & Apperley, D. C. Chain conformation in concentrated pectic gels — evidence from 13C NMR. Carbohyd. Res. 275, 131–145 (1995). This solid-state NMR study identifies homogalacturonan conformational changes after incubation with calcium.

    CAS  Article  Google Scholar 

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This is a summary of: Temple, H. et al. Golgi-localized putative S-adenosyl methionine transporters required for plant cell wall polysaccharide methylation. Nat. Plants https://doi.org/10.1038/s41477-022-01156-4 (2022).

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Shutting the door on polysaccharide methylation. Nat. Plants 8, 609–610 (2022). https://doi.org/10.1038/s41477-022-01159-1

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