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Stress-induced reactive oxygen species compartmentalization, perception and signalling

Nature Plants volume 7, pages 403–412 (2021)Cite this article

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Abstract

Reactive oxygen species (ROS) are essential for life and are involved in the regulation of almost all biological processes. ROS production is critical for plant development, response to abiotic stresses and immune responses. Here, we focus on recent discoveries in ROS biology emphasizing abiotic and biotic stress responses. Recent advancements have resulted in the identification of one of the first sensors for extracellular ROS and highlighted waves of ROS production during stress signalling in Arabidopsis. Enzymes that produce ROS, including NADPH oxidases, exhibit precise regulation through diverse post-translational modifications. Discoveries highlight the importance of both amino- and carboxy-terminal regulation of NADPH oxidases through protein phosphorylation and cysteine oxidation. Here, we discuss advancements in ROS compartmentalization, systemic ROS waves, ROS sensing and post-translational modification of ROS-producing enzymes and identify areas where foundational gaps remain.

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Fig. 1: Biphasic ROS production and cell-to-cell communication.
Fig. 2: ROS perception by the HPCA1 receptor.
Fig. 3: Conservation of the NOX C terminus across plants and of critical residues in RBOHD and human NOX5.

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Data availability

The data used to generate Fig. 3a,b and Supplementary Fig. 1a,b, as well as detailed methodology and scripts, are available in the GitHub repository (https://github.com/DanielleMStevens/ROS_production_review). The NOX C-terminal alignments are available in wasabi (http://was.bi?id=gedS1F).

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Acknowledgements

B.C. and G.C. are supported by a grant from the National Institutes of Health (NIH 1R35GM136402). B.C. is partially supported by the University of California, Davis Dean’s Distinguished Graduate Fellowship. M.W. acknowledges funding from the Academy of Finland (Decision 323917). S.K. is supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI (grant 20K05831).

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Author notes

  1. These authors contributed equally: Bardo Castro, Matteo Citterico, Sachie Kimura.

Authors and Affiliations

  1. Department of Plant Pathology, University of California, Davis, Davis, CA, USA

    Bardo Castro, Danielle M. Stevens & Gitta Coaker

  2. Organismal and Evolutionary Biology Research Programme, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland

    Matteo Citterico & Michael Wrzaczek

  3. Ritsumeikan Global Innovation Research Organization, Ritsumeikan University, Kusatsu, Japan

    Sachie Kimura

  4. Institute of Plant Molecular Biology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic

    Michael Wrzaczek

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Contributions

All authors contributed to writing the manuscript and conceiving the ideas presented. G.C. and M.W. led the structure and ideas in the manuscript and finalized the writing. S.K. generated Fig. 1. B.C. generated Fig. 2 and portions of Fig. 3. D.M.S. generated Fig. 3a,b and Supplementary Fig. 1a,b. M.C. generated the models in Fig. 3 and Supplementary Fig. 1.

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Correspondence to Michael Wrzaczek or Gitta Coaker.

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Peer review information Nature Plants thanks John Hancock and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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Supplementary Fig. 1, methods and references.

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Castro, B., Citterico, M., Kimura, S. et al. Stress-induced reactive oxygen species compartmentalization, perception and signalling. Nat. Plants 7, 403–412 (2021). https://doi.org/10.1038/s41477-021-00887-0

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