Orobanche cumana (sunflower broomrape) is an obligate parasitic plant that infects sunflower roots, causing yield losses. Here, by using a map-based cloning strategy, we identified HaOr7—a gene that confers resistance to O. cumana race F—which was found to encode a leucine-rich repeat receptor-like kinase. The complete HAOR7 protein is present in resistant lines of sunflower and prevents O. cumana from connecting to the vascular system of sunflower roots, whereas susceptible lines encode a truncated protein that lacks transmembrane and kinase domains.
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All data generated or analysed during this study are included in this published article and its Supplementary Information. GeneBank accession numbers: MN219479, MN219480, MF374791, MF374792.
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We thank all of the following colleagues from LIPM (INRA, France) for their help: E. Sallet for her help in genes annotation, J. Gouzy for his advice on sequence analysis and his reviewing of the manuscript, N. Pouilly for his advice on genotyping experiments, N. Peeters (director of the Toulouse Plant Microbe Phenotyping platform), F. Devoilles and M. Khafif for their help with some phenotyping experiments in the greenhouse. We also thank L. Rieseberg (UBC, Canada) for his reading of the manuscript and for improving the writing. We thank GeT-PlaGe Genomic Platform (INRA-Toulouse, France, https://get.genotoul.fr/en/) for producing PacBio sequence data of the BAC clones. This work was supported by INRA, Syngenta seeds and the French Laboratory of Excellence project TULIP (ANR-10-LABX-41; ANR-11-IDEX-0002-02).
The authors declare no competing interests.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Figs. 1–4, Methods, Data 1–3 and legends for Supplementary Data 1–6.
Supplementary Tables 1–8.
Sequences of HanXRQChr07g0202951 on the diversity panel.
Sequences of HaOr7 (HanXRQChr07g0202981) on the diversity panel.
Sequences of HanXRQChr07g0202811 on the diversity panel.
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Duriez, P., Vautrin, S., Auriac, MC. et al. A receptor-like kinase enhances sunflower resistance to Orobanche cumana. Nat. Plants 5, 1211–1215 (2019). https://doi.org/10.1038/s41477-019-0556-z
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