A receptor-like kinase enhances sunflower resistance to Orobanche cumana

Abstract

Orobanche cumana (sunflower broomrape) is an obligate parasitic plant that infects sunflower roots, causing yield losses. Here, by using a map-based cloning strategy, we identified HaOr7—a gene that confers resistance to O. cumana race F—which was found to encode a leucine-rich repeat receptor-like kinase. The complete HAOR7 protein is present in resistant lines of sunflower and prevents O. cumana from connecting to the vascular system of sunflower roots, whereas susceptible lines encode a truncated protein that lacks transmembrane and kinase domains.

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Fig. 1: Mapping HaOr7 onto the sunflower genome.
Fig. 2: HaOr7 confers resistance by preventing O. cumana from connecting to the root vascular system.

Data availability

All data generated or analysed during this study are included in this published article and its Supplementary Information. GeneBank accession numbers: MN219479, MN219480, MF374791, MF374792.

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Acknowledgements

We thank all of the following colleagues from LIPM (INRA, France) for their help: E. Sallet for her help in genes annotation, J. Gouzy for his advice on sequence analysis and his reviewing of the manuscript, N. Pouilly for his advice on genotyping experiments, N. Peeters (director of the Toulouse Plant Microbe Phenotyping platform), F. Devoilles and M. Khafif for their help with some phenotyping experiments in the greenhouse. We also thank L. Rieseberg (UBC, Canada) for his reading of the manuscript and for improving the writing. We thank GeT-PlaGe Genomic Platform (INRA-Toulouse, France, https://get.genotoul.fr/en/) for producing PacBio sequence data of the BAC clones. This work was supported by INRA, Syngenta seeds and the French Laboratory of Excellence project TULIP (ANR-10-LABX-41; ANR-11-IDEX-0002-02).

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Authors

Contributions

P.D. contributed to statistical analysis, genotyping, sequencing and phenotyping experiments. S.V. developed BAC libraries and performed their screening. C.C. performed the sequencing of the BAC clones. S.Cauet assembled the sequences of the BAC clones. S.Carrère annotated genes from the genomic sequences. J.B. contributed to phylogenetic analysis. M.-C.B. and F.G. contributed to the crossing and growing of sunflowers. J.-C.R. contributed to the genotyping experiments. M.L.-S. contributed to field phenotyping experiments. M.C. and M.-C.A. performed microscopy experiments. P.P.-E. calculated LD. C.P. contributed to supervising the map-based cloning analysis. B.P.-V. and L.V. contributed to the phenotyping for phylogenetic analysis. H.B. supervised the genomic analysis on BAC clones. J.P. and S.M. designed all experiments and managed the project. All of the authors contributed to the writing of the manuscript.

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Correspondence to Stéphane Muños.

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The authors declare no competing interests.

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Supplementary information

Supplementary Information

Supplementary Figs. 1–4, Methods, Data 1–3 and legends for Supplementary Data 1–6.

Reporting Summary

Supplementary Table 1

Supplementary Tables 1–8.

Supplementary Data 4

Sequences of HanXRQChr07g0202951 on the diversity panel.

Supplementary Data 5

Sequences of HaOr7 (HanXRQChr07g0202981) on the diversity panel.

Supplementary Data 6

Sequences of HanXRQChr07g0202811 on the diversity panel.

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Duriez, P., Vautrin, S., Auriac, M. et al. A receptor-like kinase enhances sunflower resistance to Orobanche cumana. Nat. Plants 5, 1211–1215 (2019). https://doi.org/10.1038/s41477-019-0556-z

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