Extended Data Fig. 6: Comparison of lwd1 mutant with lwd1ttg1 double mutant. | Nature Plants

Extended Data Fig. 6: Comparison of lwd1 mutant with lwd1ttg1 double mutant.

From: TTG1 proteins regulate circadian activity as well as epidermal cell fate and pigmentation

Extended Data Fig. 6

a, CCA1:LUC luminescence measured from Col-0, lwd1, ttg1 and lwd1ttg1 seedlings. Seedlings were entrained in 12:12 light dark cycle and transferred to camera chamber on day 9. Luminescence was measured for one 12:12 light dark cycle and 96 hours in constant light. Mean luminescence shown with SEM for n = 7, except ttg1 where n = 3. FFT-NLLS was used to estimate period values implemented using Biodare 2. Student’s t test was used to identify whether genotypes were significantly different for period values with RAE<0.5. * denotes p<0.5. b, Expression analysis of LWD2 in ttg1lwd1 mutant. The graph shows mean relative expression of LWD2 in the ttg1lwd1 double mutant compared to WT in three biological replicates, data obtained by qRT-PCR with LWD2 specific primers and reference gene UBQ10. Error bars represent standard deviation on three biological replicates.

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