PIN-FORMED (PIN) transporters mediate directional, intercellular movement of the phytohormone auxin in land plants. To elucidate the evolutionary origins of this developmentally crucial mechanism, we analysed the single PIN homologue of a simple green alga Klebsormidium flaccidum. KfPIN functions as a plasma membrane-localized auxin exporter in land plants and heterologous models. While its role in algae remains unclear, PIN-driven auxin export is probably an ancient and conserved trait within streptophytes.
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The underlying data of this study are available from the corresponding author upon request.
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We thank E. D. Cooper and C. F. Delwiche for sharing the K. flaccidum transcriptome sequences before their publication and valuable suggestions; and M. Fílová and R. Granbom for technical assistance. This work was financially supported by the Ministry of Education, Youth and Sports of Czech Republic (MEYS CR) under the projects NPU I, MSM/LO1417 (R.S., S.V. and J.P.), CEITEC 2020, LQ1601 (T.N. and M.Z.), CZ.02.1.01/0.0/0.0/16_019/0000827 (M.K.) and CZ.02.1.01/0.0/0.0/16_013/0001775 (J.P.), the Czech Science Foundation, projects GA18-26981S (J.F. and M.Z.) and GA17-17966Y (M.Z.), German Research Foundation (DFG) project HA3468/6-1 (U.Z.H.), the European Union’s Horizon 2020 programme (ERC grant agreement 742985) (J.F.) and US NSF Award DEB-1036506. T.N., M.Z. and E.M. acknowledge the CELLIM Core Facility of CEITEC and R.S., P.S. and J.P. acknowledge the imaging facility of IEB CAS, both supported by the Czech-BioImaging large RI project (LM2015062 funded by MEYS CR). T.N., M.Z. and E.M. acknowledge the Plant Sciences Core Facility of CEITEC MU. K.L. and M.K. acknowledge the Knut and Alice Wallenberg Foundation, the Swedish Governmental Agency for Innovation Systems (VINNOVA), the Swedish Research Council (VR) and the Swedish Metabolomics Centre.
The authors declare no competing interests.
Peer review information Nature Plants thanks Bert van Duijn and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.
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Skokan, R., Medvecká, E., Viaene, T. et al. PIN-driven auxin transport emerged early in streptophyte evolution. Nat. Plants 5, 1114–1119 (2019). https://doi.org/10.1038/s41477-019-0542-5