Synthetic biology is here to stay and will transform agriculture if given the chance. The huge challenges facing food, fuel and chemical production make it vital to give synthetic biology that chance—notwithstanding the shifts in mindset, training and infrastructure investment this demands. Here, we assess opportunities for agricultural synthetic biology and ways to remove barriers to their realization.
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Beddington, J. Food, energy, water and the climate: a perfect storm of global events? (UK Government, 2009).
Goold, H. D., Wright, P. & Hailstones, D. Emerging opportunities for synthetic biology in agriculture. Genes 9, 341 (2018).
Dorit, R. The biology of what is not there. Am. Sci. 99, 20 (2011).
Way, J. C., Collins, J. J., Keasling, J. D. & Silver, P. A. Integrating biological redesign: where synthetic biology came from and where it needs to go. Cell 157, 151–161 (2014).
Revolutionizing Agriculture with Synthetic Biology (The Banbury Center, Cold Spring Harbor Laboratory, 2018); https://www.cshl.edu/wp-content/uploads/2018/12/PLANT18_Program.pdf
Simon, A. J., d’Oelsnitz, S. & Ellington, A. D. Synthetic evolution. Nat. Biotechnol. 37, 730–743 (2019).
Engqvist, M. K. M. & Rabe, K. S. Applications of protein engineering and directed evolution in plant research. Plant Physiol. 179, 907–917 (2019).
Bar-Even, A. Daring metabolic designs for enhanced plant carbon fixation. Plant Sci. 273, 71–83 (2018).
Tokuriki, N. et al. Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme. Nat. Commun. 3, 1257 (2012).
Davidi, D., Longo, L. M., Jabłońska, J., Milo, R. & Tawfik, D. S. A bird’s-eye view of enzyme evolution: chemical, physicochemical, and physiological considerations. Chem. Rev. 118, 8786–8797 (2018).
Erb, T. J., Jones, P. R. & Bar-Even, A. Synthetic metabolism: metabolic engineering meets enzyme design. Curr. Opin. Chem. Biol. 37, 56–62 (2017).
de Lorenzo, V. et al. The power of synthetic biology for bioproduction, remediation and pollution control. EMBO Rep. 19, e45658 (2018).
South, P. F., Cavanagh, A. P., Liu, H. W. & Ort, D. R. Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field. Science 363, eaat9077 (2019).
Kubis, A. & Bar-Even, A. Synthetic biology approaches for improving photosynthesis. J. Exp. Bot. 70, 1425–1433 (2019).
Amthor, J. et al. Engineering strategies to boost crop productivity by cutting respiratory carbon loss. Plant Cell 31, 297–314 (2019).
Wright, R. C. & Nemhauser, J. Plant synthetic biology: quantifying the “known unknowns” and discovering the “unknown unknowns”. Plant Physiol. 179, 885–893 (2019).
Park, S. Y. et al. Agrochemical control of plant water use using engineered abscisic acid receptors. Nature 520, 545–548 (2015).
Liu, W. & Stewart, C. N. Jr. Plant synthetic biology. Trends Plant Sci. 20, 309–317 (2015).
Galanie, S., Thodey, K., Trenchard, I. J., Filsinger Interrante, M. & Smolke, C. D. Complete biosynthesis of opioids in yeast. Science 349, 1095–1100 (2015).
Wang, C., Zheng, P. & Chen, P. Construction of synthetic pathways for raspberry ketone production in engineered Escherichia coli. Appl. Microbiol. Biotechnol. 103, 3715–3725 (2019).
Liu, W. C., Gong, T. & Zhu, P. Advances in exploring alternative Taxol sources. RSC Adv. 6, 48800–48809 (2016).
Cazimoglu, I. et al. Developing a graduate training program in Synthetic Biology: SynBioCDT. Synth. Biol. 4, ysz006 (2019).
Delebecque, C. & Philp, J. Training for synthetic biology jobs in the new bioeconomy. Science https://www.sciencemag.org/careers/2015/06/training-synthetic-biology-jobs-new-bioeconomy (2015).
Niehaus, T. D., Thamm, A. M., de Crécy-Lagard, V. & Hanson, A. D. Proteins of unknown biochemical function: a persistent problem and a roadmap to help overcome it. Plant Physiol. 169, 1436–1442 (2015).
Richard Feynman Wikiquote https://en.wikiquote.org/wiki/Richard_Feynman (2019).
Zhao, Y. et al. Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane. Plant Mol. Biol. 100, 247–263 (2019).
Hillson, N. et al. Building a global alliance of biofoundries. Nat. Commun. 10, 2040 (2019).
Vazquez-Vilar, M., Orzaez, D. & Patron, N. DNA assembly standards: setting the low-level programming code for plant biotechnology. Plant Sci. 273, 33–41 (2018).
Growing the Future (UK Plant Sciences Federation, 2019); https://www.rsb.org.uk/policy/groups-and-committees/ukpsf/about-ukpsf/growing-the-future-report
Agapakis, C. M. Designing synthetic biology. ACS Synth. Biol. 3, 121–128 (2014).
Agapakis, C. Reviving the smell of extinct plants Ginko Bioworks https://www.ginkgobioworks.com/2019/05/03/reviving-the-smell-of-extinct-plants/ (2019).
de Lorenzo, V. & Schmidt, M. The do-it-yourself movement as a source of innovation in biotechnology – and much more. Microb. Biotechnol. 10, 517–519 (2017).
Bassham, J. A., Benson, A. A. & Calvin, M. The path of carbon in photosynthesis. J. Biol. Chem. 185, 781–787 (1950).
Raven, J. A., Cockell, C. S. & De La Rocha, C. S. The evolution of inorganic carbon concentrating mechanisms in photosynthesis. Philos. Trans. R. Soc. Lond. B Biol. Sci. 363, 2641–2650 (2008).
Berg, I. A., Kockelkorn, D., Buckel, W. & Fuchs, G. A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea. Science 318, 1782–1786 (2007).
Zarzycki, J., Brecht, V., Muller, M. & Fuchs, G. Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2 fixation cycle in Chloroflexus aurantiacus. Proc. Natl Acad Sci. USA 106, 21317–21322 (2009).
Figueroa, I. A. et al. Metagenomics-guided analysis of microbial chemolithoautotrophic phosphite oxidation yields evidence of a seventh natural CO2 fixation pathway. Proc. Natl Acad. Sci. USA 115, E92–E101 (2018).
Ragsdale, S. W. & Pierce, E. Acetogenesis and the Wood-Ljungdahl pathway of CO2 fixation. Biochim. Biophys. Acta 1784, 1873–1898 (2008).
Evans, M. C. W., Buchanan, B. B. & Arnon, D. I. A new ferredoxin-dependent carbon reduction cycle in a photosynthetic bacterium. Proc. Natl Acad. Sci. USA 55, 928–934 (1966).
Huber, H. et al. A dicarboxylate/4-hydroxybutyrate autotrophic carbon assimilation cycle in the hyperthermophilic Archaeum Ignicoccus hospitalis. Proc. Natl Acad. Sci. USA 105, 7851–7856 (2008).
Trudeau, D. L. et al. Design and in vitro realization of carbon-conserving photorespiration. Proc. Natl Acad. Sci. USA 115, E11455–E11464 (2018).
Bar-Even, A., Noor, E., Lewis, N. E. & Milo, R. Design and analysis of synthetic carbon fixation pathways. Proc. Natl Acad. Sci. USA 107, 8889–8894 (2010).
Schwander, T. et al. A synthetic pathway for the fixation of carbon dioxide in vitro. Science 354, 900–904 (2016).
Durall, C., Rukminasaria, N. & Lindblad, P. Enhanced growth at low light intensity in the cyanobacterium Synechocystis PCC 6803 by overexpressing phosphoenolpyruvate carboxylase. Algal Res. 16, 275–281 (2016).
This paper was inspired by discussions at the Banbury think-tank meeting, ‘Revolutionizing Agriculture with Synthetic Biology’, held at the Banbury Center, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 2–5 December, 2018. We thank the Cold Spring Harbor Laboratory Banbury Center for hosting the meeting and the Cold Spring Harbor Laboratory Corporate Sponsor Program for funding the meeting. We thank the following meeting presenters for their contributions to the thought-provoking discussions: G. Barbier, D. Bhaya, R. Bock, H. J. Bouwmeester, N. Boyle, M. Cooper, T. J. Erb, S. L. Evans, J. Gershenzon, A. D. Hanson, J. Haseloff, P. J. Hines, A. J. Kinney, S. P. Long, J. L. Matos, J. I. Medford, A. H. Millar, B. L. Møller, T. Muranaka, J. L. Nemhauser, L. Nielsen, P. I. Nikel, D. Orzáez, A. Osbourn, N. J. Patron, P. Rabinowicz, E. S. Sattely, J. Shanklin, C. Vickers and E. T. Wurtzel. Corresponding author A.D.H. acknowledges support from the US National Science Foundation (grant no. MCB-1611711).
The authors declare no competing interests.
Peer review information Nature Plants thanks Jenny Mortimer, Neal Stewart and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.
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Wurtzel, E.T., Vickers, C.E., Hanson, A.D. et al. Revolutionizing agriculture with synthetic biology. Nat. Plants 5, 1207–1210 (2019) doi:10.1038/s41477-019-0539-0