Light-harvesting antenna systems in photosynthetic organisms harvest solar energy and transfer it to the photosynthetic reaction centres to initiate charge-separation and electron-transfer reactions. Diatoms are one of the important groups of oxyphototrophs and possess fucoxanthin chlorophyll a/c-binding proteins (FCPs) as light harvesters. The organization and association pattern of FCP with the photosystem II (PSII) core are unknown. Here we solved the structure of PSII–FCPII supercomplexes isolated from a diatom, Chaetoceros gracilis, by single-particle cryoelectron microscopy. The PSII–FCPII forms a homodimer. In each monomer, two FCP homotetramers and three FCP monomers are associated with one PSII core. The structure reveals a highly complicated protein–pigment network that is different from the green-type light-harvesting apparatus. Comparing these two systems allows the identification of energy transfer and quenching pathways. These findings provide structural insights into not only excitation-energy transfer mechanisms in the diatom PSII–FCPII, but also changes of light harvesters between the red- and green-lineage oxyphototrophs during evolution.
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Atomic coordinates and Cyro-EM maps for the reported structure of C2S2, C2S1M1 and C2S2M2 have been deposited in the Protein Data Bank under accession codes 6J3Y, 6J3Z and 6J40, respectively, and in the Electron Microscopy Data Bank under accession codes EMD-9775, EMD-9776 and EMD-9777, respectively.
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This work was supported by the Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED under Grant No. JP18am0101072j0001 (to N.M.), PRESTO from JST Grant No. JPMJPR16P1 (to F.A.), JSPS KAKENHI Nos. JP17K07442 (to R.N.), JP16H06553 (to S.A.) and JP17H06433 (to J.-R.S.), Advanced Low Carbon Technology Research and Development Program from the Japan Science and Technology Agency Grant No. JPMJAL1105 (to Y.K. and K.I.) and a Collaborative Research Program from National Institute for Basic Biology Grant No. 18-451 (to K.I.). We thank H. Nishide, NIBB, for supporting the genome data analysis.
The authors declare no competing interests.
Peer review information: Nature Plants thanks Douglas Campbell and Paul Jensen and other, anonymous, reviewers for their contribution to the peer review of this work
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Nagao, R., Kato, K., Suzuki, T. et al. Structural basis for energy harvesting and dissipation in a diatom PSII–FCPII supercomplex. Nat. Plants 5, 890–901 (2019). https://doi.org/10.1038/s41477-019-0477-x
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