The molecular mechanisms underlying mycorrhizal symbioses, the most ubiquitous and impactful mutualistic plant–microbial interaction in nature, are largely unknown. Through genetic mapping, resequencing and molecular validation, we demonstrate that a G-type lectin receptor-like kinase (lecRLK) mediates the symbiotic interaction between Populus and the ectomycorrhizal fungus Laccaria bicolor. This finding uncovers an important molecular step in the establishment of symbiotic plant–fungal associations and provides a molecular target for engineering beneficial mycorrhizal relationships.
Access optionsAccess options
Subscribe to Journal
Get full journal access for 1 year
only $5.42 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Whitham, T. G. et al. Science 320, 492–495 (2008).
Vozzo, J. A. & Hacskaylo, E. Bull. Torre. Bot. Club 101, 182–186 (1974).
Gottel, N. R. et al. Appl. Environ. Microbiol. 77, 5934–5944 (2011).
Tuskan, G. A. et al. Science 313, 1596–1604 (2006).
Martin, F. et al. Nature 452, 88–92 (2008).
Martin, F., Kohler, A., Murat, C., Veneault-Fourrey, C. & Hibbett, D. S. Nat. Genet. 47, 410–415 (2015).
Kohler, A. et al. Nat. Genet. 47, 410–415 (2015).
Tagu, D. et al. Mycorrhiza 10, 237–240 (2001).
Labbé, J. et al. Tree Genet. Genomes 7, 617–627 (2011).
Geraldes, A. et al. Mol. Ecol. Resour. 13, 306–323 (2013).
Muchero, W., Labbé, J., Ranjan, P., DiFazio, S. & Tuskan, G. A. in Challenges and Opportunities for the World’s Forests in the 21st Century Vol. 81 (ed. Fenning, T.) 587–595 (Springer Netherlands, 2014).
Slavov, G. T. et al. New Phytol. 196, 713–725 (2012).
Evans, L. M. et al. Nat. Genet. 46, 1089–1096 (2014).
Muchero, W. et al. BMC Genom. 16, 24 (2015).
Plett, J. M. et al. New Phytol. 202, 270–286 (2014).
Tschaplinski, T. J. et al. Mol. Plant Microbe Interact. 27, 546–556 (2014).
Vaid, N., Macovei, A. & Tuteja, N. Mol. Plant 6, 1405–1418 (2013).
Singh, P. & Zimmerli, L. Front. Plant Sci. 4, 124 (2013).
Müller, A., Volmer, K., Mishra-Knyrim, M. & Polle, A. Front. Plant Sci. 4, 332 (2013).
Hacquard, S. et al. Nat. Commun. 7, 11362 (2016).
Felten, J. et al. Plant Physiol. 151, 1991–2005 (2009).
Plett, J. M. et al. Curr. Biol. 21, 1197–1203 (2011).
Wu, Y. & Zhou, J. M. J. Integr. Plant Biol. 55, 1271–1286 (2013).
Antolín-Llovera, M. et al. New Phytol. 204, 791–802 (2014).
Ranf, S. et al. Nat. Immunol. 16, 426–433 (2015).
Kumar, S., Nei, M., Dudley, J. & Tamura, K. Brief. Bioinform. 9, 299–306 (2008).
Van Ooijen, J. & Kyazma, B. MapQTL 6 (Kyazma BV, 2009).
Kosambi, D. D. Ann. Eugen. 12, 172–175 (1943).
Nakagawa, T. et al. Plant Biotechnol. 26, 275–284 (2009).
We thank M. Kirst at the University of Florida for allowing us to use the P. deltoides population. This work was supported by the Plant-Microbe Interfaces Scientific Focus Area in the Genomic Science Program, the Office of Biological and Environmental Research in the US Department of Energy (DOE) Office of Science. The transcriptomics work was supported by the BioEnergy Science Center (BESC) and the Center for Bioenergy Innovation (CBI). BESC and CBI are US DOE Bioenergy Research Centers supported by the Office of Biological and Environmental Research in the DOE Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC., for the US DOE under contract No. DE-AC05-00OR22725. The work conducted by the US DOE Joint Genome Institute was supported by the Office of Science of the US DOE under contract No. DE-AC02-05CH11231. W.M. was supported in part by the DOE Office of Science Early Career Research Program under the Biological and Environmental Research programme office. J.L. and F.L.T. were supported in part by a visiting grant from the Laboratory of Excellence ARBRE (No. ANR-11-LABX-0002-01) and the Region Lorraine Research Council. K.Z., J.W., X.W. and H.W. were partially supported by visiting scholarships from the China Scholarship Council. Part of the work was performed at the Oak Ridge Leadership Computing Facility at the Oak Ridge National Laboratory. The views expressed in this article do not necessarily represent the views of the US Department of Energy or the US government.
The strategy for genetic engineering of host–microbe symbiosis into non-host organisms based on the discovery described in this paper has been included in a patent application filed by UT-Battelle, LLC.
Peer review information: Nature Plants thanks Mika Tarkka and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.