Plants have evolved sophisticated systems in response to environmental changes, and growth arrest is a common strategy used to enhance stress tolerance. Despite the growth–survival trade-off being essential to the shaping of plant productivity, the mechanisms balancing growth and survival remain largely unknown. Aquaporins play a crucial role in growth and stress responses by controlling water transport across membranes. Here, we show that RhPIP2;1, an aquaporin from rose (Rosa sp.), interacts with a membrane-tethered MYB protein, RhPTM. Water deficiency triggers nuclear translocation of the RhPTM C terminus. Silencing of RhPTM causes continuous growth under drought stress and a consequent decrease in survival rate. RNA sequencing (RNA-seq) indicated that RhPTM influences the expression of genes related to carbohydrate metabolism. Water deficiency induces phosphorylation of RhPIP2;1 at Ser 273, which is sufficient to promote nuclear translocation of the RhPTM C terminus. These results indicate that the RhPIP2;1-RhPTM module serves as a key player in orchestrating the trade-off between growth and stress survival in Rosa.
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The data that support the findings of this study are available from the corresponding author upon request. All primers used in this study are described in Supplementary Table 3. Information on the genes used in this study is given in Supplementary Table 4. RNA-Seq data that support the findings of this study have been deposited in the NCBI Bioproject database under the accession number PRJNA486271 (https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA486271). The proteomic data of validation for anti-RhPTM antibody and RhPIP2;1 phosphorylation have been deposited in the PRIDE archive (Nos. PXD011942 and PXD011943, respectively; https://www.ebi.ac.uk/pride/archive).
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We thank Z. Li (Biological Mass Spectrometry Laboratory of the Functional Genomic Technology Centre of China Agricultural University) for LC–MS experiments. We thank J. Zhu (Jingjie PTM Biolabs, Inc.) for analysis of LC–MS data. We thank Z. Gong (China Agricultural University) for providing the pSuper1300 vector. We thank D. Zhang (Tsinghua University) for his generous help with MYTH screening. We thank PlantScribe (www.plantscribe.com) for careful editing of this article. This work was supported by the National Natural Science Foundation of China (grant Nos. 31520103913, 31522049, 31730079 and 31401914) and the 111 Project of the Ministry of Education (No. B17043).
The authors declare no competing interests.
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Supplementary Figures 1–10, Supplementary Methods, Supplementary References, legends for Supplementary Tables and Datasets, and Supplementary Tables 1–3.
The entire database search results for validation of anti-RhPTM antibody by LC–MS.
Differentially expressed genes in RhPTM-silenced rose plants.
The entire database search results for phosphorylation of RhPIP2;1 by LC–MS.
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Zhang, S., Feng, M., Chen, W. et al. In rose, transcription factor PTM balances growth and drought survival via PIP2;1 aquaporin. Nat. Plants 5, 290–299 (2019). https://doi.org/10.1038/s41477-019-0376-1
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