Genome editing holds great promise for increasing crop productivity, and there is particular interest in advancing breeding in orphan crops, which are often burdened by undesirable characteristics resembling wild relatives. We developed genomic resources and efficient transformation in the orphan Solanaceae crop ‘groundcherry’ (Physalis pruinosa) and used clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein-9 nuclease (Cas9) (CRISPR–Cas9) to mutate orthologues of tomato domestication and improvement genes that control plant architecture, flower production and fruit size, thereby improving these major productivity traits. Thus, translating knowledge from model crops enables rapid creation of targeted allelic diversity and novel breeding germplasm in distantly related orphan crops.
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Raw data from this study have been submitted to the National Center for Biotechnology Information Sequence Read Archive (http://www.ncbi.nlm.nih.gov/sra) under accession number SRP142654. Transcriptome and genome assemblies have been deposited at the Sol Genomics Network (ftp://ftp.solgenomics.net).
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We thank members of the Lippman and Van Eck laboratories for valuable discussions. We thank T. Mulligan, S. Vermylen, A. Krainer and S. Qiao for assistance with plant care. We thank C. Pei for assistance with CRISPR analysis. This research was supported by a National Science Foundation Postdoctoral Research Fellowship in Biology grant (IOS-1523423) to Z.H.L., and the National Science Foundation Plant Genome Research Program (IOS-1732253) to J.V.E. and Z.B.L.
The authors declare no competing interests.
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Methods, Supplementary Figure 1 and Supplementary Table 1
Raw quantification data for Figure 2 and Supplementary Figure 1
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Lemmon, Z.H., Reem, N.T., Dalrymple, J. et al. Rapid improvement of domestication traits in an orphan crop by genome editing. Nature Plants 4, 766–770 (2018). https://doi.org/10.1038/s41477-018-0259-x
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