BRZ-INSENSITIVE-LONG HYPOCOTYL 1 (BIL1)/BRASSINAZOLE-RESISTANT 1 (BZR1) is a master transcription factor of brassinosteroid (BR) signalling. The varieties of nucleobase recognition of the NN-BRRE-core motif (NNCGTG), one of variant G-box motifs, distinguish BIL1/BZR1 from basic helix-loop-helix transcription factors, underlying the specific regulation of BR-responsive genes. Here, we show the non-canonical bHLH dimer formation of BIL1/BZR1 to optimize the interaction network with DNA and the orientation of a key residue for NN-BRRE-core motif recognition.
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The atomic coordinates and structural factors are available from the PDB under accession code 5ZD4 for the mutant MBP-fused BIL1/BZR1-DNA complex. The other data that support the findings of this study are available from the corresponding author on request.
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The synchrotron-radiation experiments were performed on beamlines AR NE3A at the Photon Factory with the approval of the High Energy Accelerator Research Organization (Proposal No. 2016G648). This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas No. 17H05835 from the Japan Society for the Promotion of Science (JSPS) (T.M.), the Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) (M.T.), the Basis for Supporting Innovative Drug Discovery and Life Science Research from the MEXT (T.M.), and the Core Research for Evolutional Science and Technology (CREST) Program of Japan Science and Technology Agency (JST) (T.A. and T.N.).
The authors declare no competing interests.
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Nosaki, S., Miyakawa, T., Xu, Y. et al. Structural basis for brassinosteroid response by BIL1/BZR1. Nature Plants 4, 771–776 (2018). https://doi.org/10.1038/s41477-018-0255-1
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