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Precision genome engineering through adenine base editing in plants

An Author Correction to this article was published on 23 August 2018

This article has been updated


The recent development of adenine base editors (ABEs) has enabled efficient and precise A-to-G base conversions in higher eukaryotic cells. Here, we show that plant-compatible ABE systems can be successfully applied to protoplasts of Arabidopsis thaliana and Brassica napus through transient transfection, and to individual plants through Agrobacterium-mediated transformation to obtain organisms with desired phenotypes. Targeted, precise A-to-G substitutions generated a single amino acid change in the FT protein or mis-splicing of the PDS3 RNA transcript, and we could thereby obtain transgenic plants with late-flowering and albino phenotypes, respectively. Our results provide ‘proof of concept’ for in planta ABE applications that can lead to induced neo-functionalization or altered mRNA splicing, opening up new avenues for plant genome engineering and biotechnology.

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Fig. 1: Comprehensive A-to-G conversion efficiencies in transient assays using plant protoplasts.
Fig. 2: Analyses of in planta ABE applications.

Change history

  • 23 August 2018

    In Supplementary Fig. 1b originally published with this Brief Communication, the DNA sequence of nickase Cas9 was incorrect; this has now been amended.


  1. 1.

    Yin, K., Gao, C. & Qiu, J.-L. Nat. Plants 3, 17107 (2017).

    CAS  Article  Google Scholar 

  2. 2.

    Woo, J. W. et al. Nat. Biotechnol. 33, 1162–1164 (2015).

    CAS  Article  Google Scholar 

  3. 3.

    Alonso-Blanco, C. et al. Plant Cell 21, 1877–1896 (2009).

    CAS  Article  Google Scholar 

  4. 4.

    Slade, A. J., Fuerstenberg, S. I., Loeffler, D., Steine, M. N. & Facciotti, D. Nat. Biotechnol. 23, 75–81 (2005).

    CAS  Article  Google Scholar 

  5. 5.

    Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A. & Liu, D. R. Nature 533, 420–424 (2016).

    CAS  Article  Google Scholar 

  6. 6.

    Nishida, K. et al. Science 353, 1248–1257 (2016).

    CAS  Article  Google Scholar 

  7. 7.

    Zong, Y. et al. Nat. Biotechnol. 35, 438–440 (2017).

    CAS  Article  Google Scholar 

  8. 8.

    Shimatani, Z. et al. Nat. Biotechnol. 35, 441–443 (2017).

    CAS  Article  Google Scholar 

  9. 9.

    Gaudelli, N. M. et al. Nature 551, 464–471 (2017).

    CAS  Article  Google Scholar 

  10. 10.

    Bae, S., Park, J. & Kim, J. S. Bioinformatics 30, 1473–1475 (2014).

    CAS  Article  Google Scholar 

  11. 11.

    Kim, D. et al. Nat. Methods 12, 237–243 (2015).

    CAS  Article  Google Scholar 

  12. 12.

    Yan, L. et al. Mol. Plant 8, 1820–1823 (2015).

    CAS  Article  Google Scholar 

  13. 13.

    Tsutsui, H. & Higashiyama, T. Plant Cell Physiol. 58, 46–56 (2017).

    CAS  Article  Google Scholar 

  14. 14.

    Hanzawa, Y., Money, T. & Bradley, D. Proc. Natl Acad. Sci. USA 102, 7748–7753 (2005).

    CAS  Article  Google Scholar 

  15. 15.

    Qin, G. et al. Cell Res. 17, 471–482 (2007).

    CAS  Article  Google Scholar 

  16. 16.

    Hua, K., Tao, X., Yuan, F., Wang, D. & Zhu, J.-K. Mol. Plant 11, 627–630 (2018).

    CAS  Article  Google Scholar 

  17. 17.

    Yan, F. et al. Mol. Plant 11, 631–634 (2018).

    CAS  Article  Google Scholar 

  18. 18.

    Kim, H. et al. J. Integr. Plant Biol. 58, 705–712 (2016).

    CAS  Article  Google Scholar 

  19. 19.

    Zhang, X., Henriques, R., Lin, S. S., Niu, Q. W. & Chua, N. H. Nat. Protoc. 1, 641–646 (2006).

    CAS  Article  Google Scholar 

  20. 20.

    Park, J., Lim, K., Kim, J.-S. & Bae, S. Bioinformatics 33, 286–288 (2017).

    CAS  Article  Google Scholar 

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We thank all members of the Center for Genome Engineering for their support. We thank J. Suh for comments on the manuscript and technical assistance. This work was supported by the research fund from the Institute for Basic Science, Republic of Korea (IBS-R021-D1).

Author information




J.-Y.Y., B.-C.K., S.-T.K. and J.W.W. designed the experiments. B.-C.K., J.-Y.Y., J.R., Y.S. and M.C. generated all the constructs. B.-C.K. performed the transient assay in protoplasts. J.-Y.Y., Y.S. and J.R. generated the transgenic Arabidopsis and analysed the plants. J.-Y.Y., S.-T.K., B.-C.K. and J.-S.K. wrote the manuscript with the help of all other authors. J.-S.K. supervised the project.

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Correspondence to Jin-Soo Kim.

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J.-S.K. is a co-founder of and holds stocks in ToolGen, Inc. All other authors declare no competing interests.

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Supplementary Figures 1–8, Supplementary Table 1

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Kang, BC., Yun, JY., Kim, ST. et al. Precision genome engineering through adenine base editing in plants. Nature Plants 4, 427–431 (2018).

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