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PORCUPINE regulates development in response to temperature through alternative splicing

Nature Plants volume 4, pages 534–539 (2018)Cite this article

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Abstract

Recent findings suggest that alternative splicing has a critical role in controlling the responses of plants to temperature variations. However, alternative splicing factors in plants are largely uncharacterized. Here we establish the putative splice regulator, PORCUPINE (PCP), as temperature-specific regulator of development in Arabidopsis thaliana. Our findings point to the misregulation of WUSCHEL and CLAVATA3 as the possible cause for the meristem defects affecting the pcp-1 loss-of-function mutants at low temperatures.

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Fig. 1: PCP is essential for development at low temperature.
Fig. 2: Effects of pcp-1 on the transcriptome of A. thaliana seedlings.

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Acknowledgements

We thank J. Berger for assistance with scanning electron microscopy, N. Davidson for help with the initial RNA-seq analyses, A. Mangilet for sharing U1-70K plasmids and E. Scacchi for discussion. Supported by the DFG through the Sonderforschungsbereich 1101 (Collaborative Research Centre 1101), project SFB1101/1-C04 and the Knut and Alice Wallenberg Foundation (KAW 2016.0025) to M.S.

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Authors and Affiliations

  1. Max Planck Institute for Developmental Biology, Department of Molecular Biology, Tübingen, Germany

    Giovanna Capovilla, Silvio Collani, Ilja Bezrukov, Efthymia Symeonidi & Markus Schmid

  2. Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University for Agricultural Sciences, Umeå, Sweden

    Nicolas Delhomme

  3. Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, Sweden

    Silvio Collani, Iryna Shutava & Markus Schmid

  4. Centre for Plant Molecular Biology (ZMBP), University of Tübingen, Tübingen, Germany

    Marcella de Francisco Amorim & Sascha Laubinger

  5. Institute for Biology and Environmental Sciences (IBU), University of Oldenburg, Oldenburg, Germany

    Sascha Laubinger

Authors
  1. Giovanna Capovilla
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  2. Nicolas Delhomme
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  6. Efthymia Symeonidi
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  7. Marcella de Francisco Amorim
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Contributions

G.C. and M.S. designed the experiments. G.C. performed the RNA-seq experiments, identified and phenotyped the pcp mutants and performed RNA in situ hybridization experiments. S.C. performed the expression analysis of the RNA-seq data obtained from Col-0 grown at 16 °C, 23 °C and 27 °C. N.D. performed the alternative splicing RNA-seq analyses with help from I.S. I.B. analysed the growth rate in pcp-1 seedlings. M.d.F.A. and S.L. generated the U1-70K construct used in this study. E.S. contributed to genotyping of the transgenic lines generated by G.C. G.C. and M.S. wrote the manuscript with contributions from all authors.

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Correspondence to Markus Schmid.

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The authors declare no competing interests.

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Supplementary information

Supplementary Information

Supplementary Figures 1–7, Supplementary Tables 1–5 and legends for Supplementary Files 1–4.

Reporting Summary

Supplementary File 1

List of DE genes in Col-0 at 16°C and 27°C.

Supplementary File 2

Differential expression analyses results for pcp-1 and WT grown under different temperatures.

Supplementary File 3

Lists of DE genes between Col-0 and pcp-1.

Supplementary File 4

Curated list of meristem genes.

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Capovilla, G., Delhomme, N., Collani, S. et al. PORCUPINE regulates development in response to temperature through alternative splicing. Nature Plants 4, 534–539 (2018). https://doi.org/10.1038/s41477-018-0176-z

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