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Precision genome engineering through adenine and cytosine base editing

Nature Plantsvolume 4pages148151 (2018) | Download Citation

Abstract

Adenine base editors (ABEs), composed of an engineered deaminase and a catalytically impaired CRISPR–Cas9 variant, are powerful new tools for targeted base editing in cells and organisms. Together with cytosine base editors (CBEs), ABEs enable single-nucleotide conversions cleanly, efficiently and reversibly without double-stranded DNA cleavage, advancing genome editing in a new dimension.

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Acknowledgements

This work was supported by a grant from Institute for Basic Science (IBS-R021-D1).

Author information

Affiliations

  1. Center for Genome Engineering, Institute for Basic Science, Seoul, Republic of Korea

    • Jin-Soo Kim
  2. Department of Chemistry, Seoul National University, Seoul, Republic of Korea

    • Jin-Soo Kim

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Competing interests

J.-S.K. is a co-founder of and holds stock in ToolGen, Inc. focused on genome editing.

Corresponding author

Correspondence to Jin-Soo Kim.

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DOI

https://doi.org/10.1038/s41477-018-0115-z

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