Control of auxin-induced callus formation by bZIP59–LBD complex in Arabidopsis regeneration

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Abstract

Induction of pluripotent cells termed callus by auxin represents a typical cell fate change required for plant in vitro regeneration; however, the molecular control of auxin-induced callus formation is largely elusive. We previously identified four Arabidopsis auxin-inducible Lateral Organ Boundaries Domain (LBD) transcription factors that govern callus formation. Here, we report that Arabidopsis basic region/leucine zipper motif 59 (AtbZIP59) transcription factor forms complexes with LBDs to direct auxin-induced callus formation. We show that auxin stabilizes AtbZIP59 and enhances its interaction with LBD, and that disruption of AtbZIP59 dampens auxin-induced callus formation whereas overexpression of AtbZIP59 triggers autonomous callus formation. AtbZIP59–LBD16 directly targets a FAD-binding Berberine (FAD-BD) gene and promotes its transcription, which contributes to callus formation. These findings define the AtbZIP59–LBD complex as a critical regulator of auxin-induced cell fate change during callus formation, which provides a new insight into the molecular regulation of plant regeneration and possible developmental programs.

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Fig. 1: AtbZIP59 physically interacts with auxin-inducible LBDs.
Fig. 2: AtbZIP59 mediates auxin-inducible callus formation.
Fig. 3: CIM stabilizes AtbZIP59 and enhances its interaction with LBD16.
Fig. 4: AtbZIP59 and LBD16 act synergistically in directing callus formation.
Fig. 5: FAD-BD is targeted by the AtbZIP59–LBD16 complex.

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Acknowledgements

We are grateful to B. Scheres, J. Haseloff, N.-H. Chua, J.-W. Wang and J. Jin for providing the seeds and constructs used in this study. We thank J. M. Alonso (North Carolina State University) for critical comments on the manuscript. We acknowledge K. Yang for help in preparing some of the constructs for the co-IP experiment. This work was supported by the National Natural Science Foundation of China (grant no. 31230009 and grant no. 31771632), the Ministry of Science and Technology of China (grant no. 2013CB967300) and the Strategic Priority Research Program of Chinese Academy of Sciences (grant no. XDPB0403).

Author information

Y.H. conceived the project; C.X. and Y.H. designed the experiments; C.X., H.C., H.W. and W.X. performed the experiments; Q.Z., E.X., S.Z., R.Y. and D.Y. contributed to the generation of some constructs; C.X. and Y.H. analysed the data and wrote the manuscript.

Correspondence to Yuxin Hu.

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Xu, C., Cao, H., Zhang, Q. et al. Control of auxin-induced callus formation by bZIP59–LBD complex in Arabidopsis regeneration. Nature Plants 4, 108–115 (2018) doi:10.1038/s41477-017-0095-4

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