Article

Maintenance of carbohydrate transport in tall trees

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Abstract

Trees present a critical challenge to long-distance transport because as a tree grows in height and the transport pathway increases in length, the hydraulic resistance of the vascular tissue should increase. This has led many to question whether trees can rely on a passive transport mechanism to move carbohydrates from their leaves to their roots. Although species that actively load sugars into their phloem, such as vines and herbs, can increase the driving force for transport as they elongate, it is possible that many trees cannot generate high turgor pressures because they do not use transporters to load sugar into the phloem. Here, we examine how trees can maintain efficient carbohydrate transport as they grow taller by analysing sieve tube anatomy, including sieve plate geometry, using recently developed preparation and imaging techniques, and by measuring the turgor pressures in the leaves of a tall tree in situ. Across nine deciduous species, we find that hydraulic resistance in the phloem scales inversely with plant height because of a shift in sieve element structure along the length of individual trees. This scaling relationship seems robust across multiple species despite large differences in plate anatomy. The importance of this scaling becomes clear when phloem transport is modelled using turgor pressures measured in the leaves of a mature red oak tree. These pressures are of sufficient magnitude to drive phloem transport only in concert with structural changes in the phloem that reduce transport resistance. As a result, the key to the long-standing mystery of how trees maintain phloem transport as they increase in size lies in the structure of the phloem and its ability to change hydraulic properties with plant height.

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Acknowledgements

Funding was provided by the National Science Foundation (IOS 1021779 and 1456682 to N.M.H. and M.K.), a Katharine H. Putnam Fellowship in Plant Science (J.A.S.), a Harvard Bullard Fellowship (M.K.), and the University of Minnesota Duluth (J.A.S.). Samples were collected at the Arnold Arboretum of Harvard University (permit no. 22-2013), the Harvard Forest and the main campus of Harvard University. Imaging was completed at the Harvard Center for Biological Imaging, Franceschi Microscopy and Imaging Center at WSU, the Center for Nanoscale Systems (Harvard University) and the Weld Hill Microscopy Lab at the Arnold Arboretum.

Author information

Affiliations

  1. Department of Biology, University of Minnesota, Duluth, MN, USA

    • Jessica A. Savage
  2. Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA

    • Jessica A. Savage
    • , Laura Clerx
    • , Jessica T. Gersony
    • , Juan M. Losada
    •  & N. Michele Holbrook
  3. School of Biological Sciences, Washington State University, Pullman, WA, USA

    • Sierra D. Beecher
    • , Jan Knoblauch
    •  & Michael Knoblauch
  4. Department of Physics, Technical University of Denmark, Lyngby, Denmark

    • Kaare H. Jensen

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Contributions

J.A.S., K.H.J., M.K. and N.M.H. designed the research. J.A.S., S.D.B., L.C., J.T.G., J.K., M.K. and J.M.L. collected the data and standardized protocols. J.A.S. and K.H.J. analysed the data. J.A.S., N.M.H., K.H.J. and M.K. wrote the paper.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Jessica A. Savage.

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