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Arabidopsis BTB/POZ protein-dependent PENETRATION3 trafficking and disease susceptibility

Abstract

The outermost cell layer of plant roots (epidermis) constantly encounters environmental challenges. The epidermal outer plasma membrane domain harbours the PENETRATION3 (PEN3)/ABCG36/PDR8 ATP-binding cassette transporter that confers non-host resistance to several pathogens. Here, we show that the Arabidopsis ENDOPLASMIC RETICULUM-ARRESTED PEN3 (EAP3) BTB/POZ-domain protein specifically mediates PEN3 exit from the endoplasmic reticulum and confers resistance to a root-penetrating fungus, providing prime evidence for BTB/POZ-domain protein-dependent membrane trafficking underlying disease resistance.

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Fig. 1: eap3 mutations affect a cytosolic BTB/POZ-domain protein.
Fig. 2: EAP3 mediates PEN3 secretory trafficking from the ER with high cargo specificity.
Fig. 3: pen3 and eap3 mutations increase susceptibility to F. oxysporum.

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Acknowledgements

We are grateful to D.W. Ehrhardt, J. Friml, N. Geldner, B. Scheres and S.C. Somerville for making available published materials used in this study, as well as to M. Lenhard for critical reading of the manuscript. We thank the Nottingham Arabidopsis Stock Centre for providing seed stocks. This work was supported by ERC consolidator grant ERC-STG-2010 No. 260699 Green-Lat-Pol to M.Gr. and by SNF grant 31003A_165877 to M.Ge.

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M.Gr. conceived the project. M.Gr., H.M., B.A. and M.Ge. designed experiments. H.M. performed all experiments except for Fusarium oxysporum work and for SHORE mapping. T.L. and J.H. performed SHORE mapping of eap3-1. B.A. and M.Ge. performed Fusarium oxysporum experiments. M.Gr. and H.M. wrote the manuscript. All authors read and edited the manuscript prior to publication.

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Correspondence to Markus Grebe.

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Supplementary Methods, Supplementary References, Supplementary Tables 1 and 2, Supplementary Figures 1–6, Supplementary Methods

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Mao, H., Aryal, B., Langenecker, T. et al. Arabidopsis BTB/POZ protein-dependent PENETRATION3 trafficking and disease susceptibility. Nature Plants 3, 854–858 (2017). https://doi.org/10.1038/s41477-017-0039-z

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  • DOI: https://doi.org/10.1038/s41477-017-0039-z

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