Abstract
Metabolic imbalance leading to inflammatory hypoxia and stabilization of hypoxia-inducible transcription factors (HIFs) is a hallmark of inflammatory bowel diseases. We hypothesize that HIF could be stabilized in CD4+ T cells during intestinal inflammation and alter the functional responses of T cells via regulation of microRNAs. Our assays reveal markedly increased T cell-intrinsic hypoxia and stabilization of HIF protein during experimental colitis. microRNA screen in primary CD4+ T cells points us towards miR-29a and our subsequent studies identify a selective role for HIF-2α in CD4-cell-intrinsic induction of miR-29a during hypoxia. Mice with T cell-intrinsic HIF-2α deletion display elevated T-bet (target of miR-29a) levels and exacerbated intestinal inflammation. Mice with miR-29a deficiency in T cells show enhanced intestinal inflammation. T cell-intrinsic overexpression of HIF-2α or delivery of miR-29a mimetic dampen TH1-driven colitis. In this work, we show a previously unrecognized function for hypoxia-dependent induction of miR-29a in attenuating TH1-mediated inflammation.
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Introduction
The intestines contain the most significant number of immune cells of any tissue in the body and are a site for considerable immunologic activity. With the central role of oxygen metabolism and the physiological characteristics of this organ, intestinal homeostasis is highly dependent on adaptive pathways activated by hypoxia-dependent transcriptional programs. During inflammatory bowel diseases (IBD), a marked increase in intestinal inflammatory hypoxia and stabilization of the HIF (hypoxia-inducible factor) transcription factors occurs due to increased metabolic demand and a paucity of available oxygen1,2,3,4,5,6,7. The increased localized hypoxia during intestinal inflammation has been attributed partially to transmigrating neutrophils, which utilize respiratory bursts to rapidly and locally deplete oxygen during inflammation8. In the inflamed mucosa, HIF signaling elicits protective immune responses, increases barrier genes, and maintains the intestine’s regenerative and proliferative capacity, leading to the resolution of inflammation9,10,11.
CD4+ helper T cells play a critical role in coordinating immune responses to pathogens. At the same time, their unrestrained activation drives chronic pathologies, which contribute centrally to the pathogenesis of inflammatory bowel diseases. Evidence for the CD4+ TH1 cell involvement in IBD inflammation comes from the prominent transmural infiltration associated with IFNγ+ granulomata12, while the TH1 effector cytokines and the cardinal TH1 transcription factor T-bet are significantly increased in active Crohn’s disease lesions13,14,15. T-bet regulates differentiation cues of TH1 T cells by directly driving TH1 responses or by repressing TH17 activation in T-bet-deficient mice within experimental models of colitis15,16. Production of IFNγ by the progeny of TH17 cells is required for disease development in a transfer model of colitis, and T-bet is indispensable for the induction of colitis, underscoring the central role for this transcription factor and for IFNγ signaling in colitic T cells15. While numerous reports explored the role of HIF factors in the development and intestinal function of TH9, TH17 and Treg cells3,17,18,19,20, our understanding of how hypoxic signaling affects TH1 T cell function in intestinal inflammation remains limited.
Functional consequences of hypoxia can lead the induction of HIF target genes promoting cell survival under hypoxic conditions (such as erythropoietin). However, in many instances, HIF stabilization is associated with the repression of target genes21, most commonly via the induction of hypoxia-inducible micro RNAs (miRNAs)22,23. miRNAs which are short, noncoding RNAs exerting post-transcriptional regulation of gene expression by targeting 3’UTR in a sequence-specific manner24 have been shown as HIF targets and were implicated in attenuating inflammation25,26,27. Additionally, miRNAs play specialized suppressive roles during helper T cell development28,29,30. A genetic deficiency in the enzymes which process miRNA transcripts into their mature form leads to deficits in CD4+ T cell functions including a bias towards IFNγ production31. Specifically, miR-29a directly regulates IFNγ expression and mice with miR-29a deficiency show increased resistance to intracellular bacterial infection but higher susceptibility to IFNγ-driven autoimmune disorders32,33.
Here, we report the functional consequences of hypoxia within the inflamed intestine on the miRNA expression in helper T cells. We develop assays to measure T cell-specific hypoxia and HIF stabilization in vivo and find that colitogenic CD4+ T cells undergo a marked increase in cellular hypoxia and stabilize HIF during active colitis. Our studies indicate that this hypoxic stimulation represses T-bet expression and IFNγ production from TH1 T cells in a HIF-2α-dependent manner. Therefore, in this work, we uncover a central role of hypoxia-signaling in the negative feedback regulation of TH1 T cell expansion during intestinal inflammation via the transcriptional induction of immunologic active miRNAs.
Results
Colitogenic CD4+ T cells become hypoxic and stabilize HIF
Tissue hypoxia is a hallmark of colitis1,8, and previous studies have shown a protective role of HIFs in attenuating intestinal inflammation34. However, the knowledge about the role of the hypoxic microenvironment on the function of T helper cells is limited. Therefore, we pursued studies to visualize and quantify T cell-intrinsic hypoxia and concomitant HIF stabilization in murine models of intestinal inflammation in vivo. To assess T cell hypoxia across varying activation states, we administered 2-nitroimidazole (EF5) intravenously into mice with naïve T cell transfer-induced colitis, and followed by anti-EF5 labeling (EF5-Cy5). Resulting hypoxic adducts in T cells at fulminant disease were then analyzed by flow cytometry. We observed that effector-memory CD4+ T cells (TEM) in inflamed colons accumulate significant EF5+ staining indicating decreased oxygen tension (hypoxia) in these cells (Fig. 1a). Similar EF5+ staining pattern is evident in the longer-lasting effector-memory T cells (Fig. 1b). Increased staining intensities were observed in the intestinal compared to mesenteric lymph node or spleen T cells, in line with the ranked physiologic hypoxic gradients of these organs during intestinal inflammation35.
Next, we designed a system to visualize the activation of T cell-intrinsic HIF stabilization within the inflamed colon utilizing adoptive transfer of naïve CD4+ T cells (CD4+CD45RBhigh) from HIF-reporter mice [“B6ΔODD-luciferase mice”36] into host mice that do not express luciferase (Supplementary Fig. 1a). These mice were fully backcrossed onto C57BL/6J/6J background to allow for a transfer into a matching Rag1-deficient strain. At fulminant disease (7 weeks post-transfer, Supplementary Fig. 1b) luciferase activity derived solely from the hypoxic T cells was measured by whole-tissue imaging. While transferring CD4+CD45RBlow T cells resulted in a normal colon appearance and background levels of luciferase (Fig. 1c), the CD4+CD45RBhigh T cell recipients exhibited severe colitis, documented by histological analysis which correlated with a marked increase in T cell-intrinsic HIF stabilization (Fig. 1d), and confirmed by measurements of the luciferase radiance signal intensity (Fig. 1e), with the colon inflammatory scores matching the luciferase and histological data (Fig. 1f). In comparison, the small intestines from the same colitic animals show similar background levels of luciferase activity (Supplementary Fig. 1c and Supplementary Fig. 1d). Taken together, the present studies demonstrate a CD4+ T cell-intrinsic increase in hypoxia with a concomitant stabilization of HIF during active colitis in mice.
miR-29a is a hypoxia target in CD4+ T cells
Based on our studies showing that CD4+ T cells experience intrinsic hypoxia and concomitant HIF stabilization in colitis, we next pursued the transcriptional consequences of HIFs stabilization. While HIF is known to induce specific target genes (such as erythropoietin), in many instances HIF stabilization is associated with the repression of target genes21, most commonly via the induction of hypoxia-inducible miRNAs22,23. Therefore, we examined T cell intrinsic transcriptional responses to ambient hypoxia on miRNA expression. For this purpose, we subjected CD4+cells to brief activation (8 h) in normal oxygen conditions or in hypoxia (1% oxygen) and performed a limited T cell-focused miRNA expression array (Supplementary Data 1). This screen identified miR-29a as the leading miRNA candidate upon ambient hypoxia exposure of purified CD4+ T cells (Fig. 2a). Mature miR-29s are highly conserved in human, mouse, and rat and share identical sequences at the seed region that plays a key role in determining their targets37. In addition, we also observed increased miR-29a expression in colonic pinch biopsies from inflammatory bowel disease (IBD) patients suffering from Crohn’s disease (CD) or Ulcerative Colitis, which correlated with disease activity (Fig. 2b). Fractionation of colon from a healthy mouse demonstrates that miR-29a expression localizes largely to the lamina propria lymphocytes (LP), with comparatively lower level of expression in the intestinal epithelial cells (IEC) (Fig. 2c).
Next, we differentiated mouse CD4+ T helper lineages (TH0, TH1, TH2, TH17, Treg) and identified a significant upregulation of miR-29a following 8 h of ambient hypoxia exposure (1% O2). This induction was highest in the TH0 and TH1 subsets (Fig. 2d). The hypoxia-driven increase in miR-29a was mirrored in whole primary human CD4+ and differentiated TH0 and TH1 CD4+ T cells cultured from peripheral blood mononuclear cells of healthy donors and subjected to similar activating regimen (Fig. 2e). Collectively, these studies identify that miR-29a is a T cell-expressed miRNA which shows increased expression during exposure to hypoxia, particularly in TH1 T cells and potentially in other T cell subsets.
Induction of miR-29a in CD4+ T cells is mediated by HIF-2α
Hif-1α and Hif-2α are closely related isoforms but genetic inactivation studies of each isoform in mice demonstrate substantially different phenotypes, suggesting that HIF-1α or HIF-2α control distinct transcriptional targets38. Therefore, we applied genetic studies to address the relative contributions of HIF-1α or HIF-2α to miR-29a induction during hypoxia. For this purpose, primary mouse CD4+ T cells from mice with T cell-intrinsic HIF isoform deletions (Hif-1αloxP/loxP Lck Cre+ or Hif-2αloxP/loxP Lck Cre+ mice18,39,40 were activated in vitro. Following 8 h of ambient hypoxia exposure (1% oxygen), miR-29a was significantly induced in T cells from control mice (Lck Cre+) compared to Hif-1α-deficient T cells. In contrast, miR-29a induction was completely abolished in Hif-2α-deficient CD4+ T cells (Fig. 3a). Conversely, CD4+ T cells from mice with T cell-intrinsic HIF-2α stabilization [(LSL-HIF2dPA Lck Cre+)41] displayed a significant induction of miR-29a under normoxia (Fig. 3b). Lastly, to assess if miR-29a induction can be achieved pharmacologically using a HIF-activator, we treated primary CD4+ T cells from mice and humans with the cell permeable prolyl-hydroxylase inhibitor, dimethyloxaloylglycine (DMOG)42. Consistent with the above studies in genetic models, DMOG treatment significantly induced miR-29a to levels comparable with the induction observed during hypoxia exposure (mouse cells Fig. 3c and human cells Fig. 3d). Furthermore, the levels of miR-29a in colonic biopsies of middle colon failed to increase in mice with Hif-2α-deficient CD4+ T cells compared to controls in a chronic DSS colitis model (Fig. 3e), although this increase may not be solely due to the T cell derived miR-29a.
Previously published analyses have confirmed that miR-29b-1 and miR-29a are transcribed together as a polycistronic primary transcript with mature miR-29a localizing to the cytoplasm while miR-29b-1 predominantly localizes to the nucleus37,43. Analysis of the nucleotide sequence within the miR-29b1-a proximal promoter identifies 7 potential Hypoxia Responsive Elements (HRE)44 and the 3 most proximal to the transcriptional start site are indicated on the diagram (Fig. 3f). We employed chromatin immunoprecipitation (ChIP) assay to address binding of HIF-2α to 3 individual proximal HREs that most closely conformed to the previously established criteria for functional HREs within 1 kb upstream of the transcriptional start site44. These studies demonstrate modest binding of HIF-2α to HRE#1 (Fig. 3g, first panel) but not to HRE#2 and HRE#3 (Fig. 3g, panels 2 and 3) during ambient hypoxia exposure of CD4+ T cells (4 more distal HREs were not tested in this assay). Together these studies point to a role for HIF-2α in the induction of miR-29a in CD4+ T cells during ambient hypoxia exposure.
miR-29a target T-bet is repressed in TH1 T cells in hypoxia
Having identified a T cell-intrinsic role of HIF-2α in the induction of miR-29a, we next pursued studies to identify specific miR-29a target genes as a functional consequence of the increased expression of this microRNA. Among previously characterized miR-29a target genes, the TH1 transcription factor T-bet (Tbx21) was shown to be a bona fide target45 and T cell-intrinsic overexpression of miR-29a negatively regulated T-bet and a closely related transcription factor, Eomes31. Naive T cells activated under hypoxic conditions (1% oxygen) for 8 h rapidly increased expression of miR-29a compared to similarly cultured cells under normoxic conditions (Fig. 4a). While normoxic activated cells significantly up-regulated T-bet expression upon activation, hypoxia exposure was not associated with T-bet increases (Fig. 4b). To study the effect of hypoxia on T-bet and its direct target IFNγ, naïve CD4+ T cells were differentiated into TH1 lineage and treated with increasing concentration of DMOG during subsequent activation. Pharmacological stabilization of HIF resulted in a diminished expression of T-bet protein (Fig. 4c) and a dose-dependent decrease of IFNγ release (Fig. 4d). Exposure of CD4+ TH1 T cells to ambient hypoxia over 24 h was associated with attenuated T-bet protein expression following T cell receptor stimulation (Fig. 4e), and a diminished cytokine release (Fig. 4f). Upon transfecting miR-29a inhibitor (antisense) into T cells, the hypoxia-mediated repression of T-bet in proliferating T cells was no longer observed (Fig. 4g, h). Conversely, T cells transfected with miR-29a mimetic downregulate T-bet upon in vitro activation under normoxia compared to control-transfected cells (Fig. 4i). This data points to a direct role for hypoxia-inducible miR-29a in the regulation of TH1 master transcription factor T-bet.
Considering the observations above we hypothesized that stabilizing HIF-2α in T cells will lead to protection during colitis by increasing the expression of miR-29a and consequently downregulating the TH1 response (including and not limited to T-bet induction and IFNγ release) and in turn, diminish the proliferation of pathogenic T cells. For this purpose, we challenged mice with T cell-intrinsic stabilized HIF-2α (LSL-HIF2dPA Lck Cre+) with 2,4,6-Trinitrobenzenesulfonic acid (TNBS) which leads to a TH1-mediated colitis. As anticipated, T cells from the mesenteric lymph node (MLN) in colitic mice with stabilized HIF-2α expression failed to up-regulate T-bet protein (Fig. 4j and Supplementary Fig. 2a). Indeed, the LSL-HIF2dPA Lck Cre+ mice displayed significantly attenuated colitis as indicated by comparatively diminished tissue damage (Fig. 4k) and lower histological scores of colon injury (Fig. 4l and Supplementary Fig. 2b). Taken together, our results provide evidence that the increased miR-29a expression in response to either hypoxia or pharmacological HIF stabilization in vitro, or in a genetic mutation that leads to HIF-2α stabilization in vivo, allows for a coordinated reduction in TH1 responses during colitis.
T cell-intrinsic HIF-2α dampens TH1-mediated colitis
As the HIF-2α stabilization in T cells and the consequent modulation of miR-29a expression affects T-bet expression in TH1 T cells, we wondered about the physiological role of HIF-2α deficiency in the transfer colitis model and the TNBS model. Isolated CD4+CD45RBhigh T cells from Hif-2αloxP/loxP Lck Cre+ mice and littermate controls were transferred into B6.RAG1-/- mice. Host animals receiving Hif-2α-deficient T cells exhibited significant weight loss compared to control mice (Fig. 5a). At fulminant disease (8 weeks post-transfer), colons were removed and assessed for different measures of disease outcomes. Consistent with a protective role for T cell-intrinsic HIF-2α, we observed significantly higher colon weight-to-length (W/L) ratio in Hif-2αloxP/loxP Lck Cre+ T cell recipients compared to control mice indicating increased severity of colonic inflammation (Fig. 5b). Mice who received Hif-2α-sufficient CD4+CD45RBlow T cells were completely protected from weight loss and colonic inflammation due to the presence of Treg cells in the CD45RBlow fraction46,47 (Fig. 5a, b) and developed no marked colon inflammation (Fig. 5c, d). Adoptive transfer of naïve CD4+ T cells from Hif-2αloxP/loxP Lck Cre+ and Lck Cre+ controls (to account for the effect of the Cre) resulted in enhanced weight loss (Supplementary Fig. 3b) and colonic inflammation in recipients of HIF-2α deficient cells (Supplementary Fig. 3c). Adoptively transferred Hif-2α-deficient T cells in MLN showed increased expression of T-bet (Supplementary Fig. 3d), but no significant differences were noted in expression of IL-17A (Supplementary Fig. 3e). Histological scores were significantly elevated in the recipients of naïve Hif-2α-deficient T cells (Fig. 5c), and representative histological colon preparations showed increased recruitment of leukocytes together with pronounced epithelial damage (Fig. 5d). Similarly, disease severity index from day 18-36 were significantly elevated in the recipients of Hif-2α-deficient T cells (Supplementary Fig. 3a). T cells isolated from the mesenteric lymph nodes of mice receiving HIF-2α-deficient CD4+CD45RBhigh T cells showed increased activation of IFNγ and T-bet (Fig. 5e and Fig. 5f, respectively), consistent with the existence of a unique regulatory pathway for TH1 T cell modulation by HIF-2α-miR-29a axis which works to restrain TH1 activation during colitis.
Next, Hif-2αloxP/loxP Lck Cre+ mice together with littermate Hif-2αloxP/loxP Lck Cre- controls were sensitized and subsequently challenged with TNBS and, in line with our in vitro data, CD4+ T cells isolated from the mesenteric lymph nodes of mice with a T cell-specific Hif-2α deletion displayed elevated expression of T-bet (Fig. 5g) and increased production of IFNγ (Fig. 5h). Heightened histological inflammatory scores indicated significantly exacerbated colitis in Hif-2αloxP/loxP Lck Cre+ but not in Hif-2αloxP/loxP Lck Cre- littermate mice (Fig. 5i). Representative colon micrographs shown in Fig. 5j illustrate increased inflammatory damage in Hif-2αloxP/loxP Lck Cre+ animals. Taken together, these studies demonstrate that HIF-2α stabilization can restrain T cell inflammatory phenotype, while HIF-2α deficiency in T cells leads to exacerbated disease in mice; therefore, providing a crucial link between inflammatory hypoxia and T lymphocytes in the context of IBD.
Functional role of miR-29a in CD4+ T cells
To study the specific role of miR-29a in T cells, we used previously described mice with a “floxed” miR-29a locus33 to generate miR-29aloxP/loxP Lck Cre+ mice and used miR-29aloxP/loxP Lck Cre- littermates as experimental controls (details of strain generation in Materials and Methods). T cell-specific deletion of miR-29a was confirmed in the total thymocytes (Supplementary Fig. 4a) and in the peripheral naïve CD4+ T cells transiently stimulated under normoxia and hypoxia (1% O2) with anti-CD3/CD28 antibodies (Fig. 6a). Additionally, Lck Cre+-derived CD4+ T cells were compared to miR-29aloxP/loxP Lck Cre+ cells and found to also significantly upregulate miR-29a under hypoxia (Supplementary Fig. 4b). Following activation for 6 h, miR-29a-deficient T cells showed increased levels of T-bet mRNA under normoxia and hypoxia (Fig. 6b), with significantly higher expression of IFNγ mRNA as compared to miR-29a-sufficient T cells (Fig. 6c). Fully differentiated miR-29a-deficient CD4+ TH1 T cells produced significantly more IFNγ mRNA than controls during 8 h activation with anti-CD3/CD28 under normoxic conditions (Fig. 6d and Supplementary Fig. 4d).
To establish a role for miR-29a in T cells during intestinal inflammation we performed adoptive transfer of naïve CD4+ CD45RBhigh T cells derived from miR-29a-sufficient (Lck Cre+) and deficient (miR-29aloxP/loxP Lck Cre+) mice into B6.RAG1-/- recipients. Unexpectedly, both groups displayed similar weight loss scores (Supplementary Fig. 4e). We then hypothesized that T cell differentiation into effector subsets may be impaired in the absence of miR-29a, and to test this we prepared in-vitro skewed TH1 T cells and adoptively transferred them into B6.RAG1-/- recipients. At 9 weeks post transfer mice receiving miR-29a-deficient T cells lost significantly less weight (Fig. 6e), although their colon inflammatory scores remained comparable to the controls (Fig. 6f). Both groups displayed similar inflammatory changes in the colon (Fig. 6g). Interestingly, analysis of T cells from the mesenteric lymph nodes showed that effector T cell differentiation is affected by the loss of miR-29a (Fig. 6h). While miR-29a-deficient T cells efficiently retained their TH1 lineage commitment and produced high amounts of IFNγ (Fig. 6i) with notably higher survival rate under hypoxia (Supplementary Fig. 4c), the miR-29a-sufficient T cells were more proficient differentiating into IL-17A-producing T cells (Fig. 6j). Indeed, purified CD4+ miR-29a-deficient T cells skewed in-vitro towards TH17 show diminished survival (Supplementary Fig. 4f and Supplementary Fig. 4g) and limited IL-17A production under hypoxia (Supplementary Fig. 4h). The loss of TH1 identity and differentiation into IL-17A+ T cells within the hypoxic intestinal environment in the transfer colitis model has been described previously48, and a report by Leppkes et al.49 identified the highly pathogenic role for IL-17A+ T cells in the transfer colitis model which results in a higher disease severity than IFNγ+ T cells alone. We conclude that in CD4+ T cells deletion of miR-29a relieves the restraint on T-bet expression and leads to better retention of TH1 identity and higher IFNγ production concomitant with an impairment in IL-17A production resulting in a diminished disease severity.
To further study the role for miR-29a in TH1 T cells during intestinal inflammation we exposed mice with T cell-specific miR-29a deletion to TNBS colitis. Consistent with a functional role of miR-29a in suppressing a Th1 response, miR-29aloxP/loxP Lck Cre+ mice lost weight at a higher rate and took longer to regain it (Fig. 6k) while their colons appeared significantly shortened compared to the littermate controls (Fig. 6l). Moreover, cumulative disease scores that account for the onset and degree of symptoms were significantly increased in animals with T cell-specific miR-29a deletion (Supplementary Fig. 5a). Colonic explants from these mice released significantly more IFNγ upon 20 h ex vivo culture, indicating higher inflammation (Fig. 6m). Importantly, flow cytometric analysis of miR-29a-deficient CD4+ T lymphocytes isolated from the lamina propria of TNBS-treated mice showed increased expression of T-bet protein compared to their littermate controls (Fig. 6n). Histological scores (Fig. 6o) and representative histological images (Fig. 6p) demonstrated a more severe inflammation, more pronounced crypt erosion and lymphocyte infiltration into the mucosal layer in the miR-29loxP/loxP Lck Cre+ but not Cre- littermate mice. To eliminate strain bias, similar experiments were performed in miR-29loxP/loxP Lck Cre+ and Lck Cre+ mice which displayed comparable weight loss pattern (Supplementary Fig. 5b), colon shrinkage and histopathology changes (Supplementary Fig. 5c, d). IFNγ production in cell culture of naïve and differentiated TH1 T cells from miR-29loxP/loxP Lck Cre+ was significantly higher than Lck Cre+ controls (Supplementary Fig. 5e), while production of another proinflammatory cytokine, TNFα, was not significantly changed in the context of T cell intrinsic miR-29a deficiency in vitro (Supplementary Fig. 5f, Supplementary Fig. 5g) or in vivo (Supplementary Fig. 5h). Taken together, these studies indicate that T cell intrinsic deletion of miR-29a is associated with enhanced hyperactivity in vitro, and concomitant increases in TH1-mediated activation and proliferation during experimental colitis.
Nanoparticle delivery of miR-29a attenuates colitis in mice
Having demonstrated a functional impact of miR-29a deletion in T cells towards unrestrained T-bet and IFNγ output resulting in enhanced inflammatory phenotype, we next pursued studies to address a potential therapeutic application of miR-29a overexpression. Small molecules targeted at aberrant inflammation pathways are the focus of intense investigation both for IBD diagnosis and treatment, and the first small-interfering RNA (siRNA) was recently approved by the FDA for the treatment of amyloidosis50. Therefore, we pursued studies to investigate the efficacy of utilizing miR-29a mimetic as a therapeutic intervention during experimental colitis.
In order to confirm that mucosa-homing T cell are able to retain microRNA mimetics, we intraperitoneally injected Cy3-labeled and liposome-packaged microRNA (tracer miRNA with no biological activity) into colitic C57BL/6J/6J mice that were previously challenged with TNBS. Mesenteric lymph nodes were subsequently isolated and assayed by flow cytometry. We observed accumulation of Cy3-positive CD4+ T cells in the mesenteric lymph node within 5 h post-injection (Supplementary Fig. 6a, b), which diminished over time indicating cell migration and microRNA turnover (Supplementary Fig. 6c).
To test the efficacy of miR-29a therapy during inflammation synthetic murine miR-29a mimetics were delivered to mice in a lipid emulsion of nanoparticles (iv, chosen as the preferred method requiring less handling time for the mimetic prior to injection) on days 1 and 3 post-TNBS administration51. As a relevant control, we used a corresponding miRNA that is restrictively expressed in C. elegans nematode [(Cel-miR-239b) (miRIDIAN microRNA Mimic Negative Control #2, Horizon Discovery Biosciences Ltd.)]. The miR-29a mimetic treatment (Fig. 7a) markedly attenuated intestinal pathology compared to a control treatment as indicated by decreased total disease index which is comprised of the inflammatory index and the injury index (Fig. 7b). Representative histological slides show significantly diminished lymphocyte infiltration and a lesser damage to the colonic lining in the mimetic-treated group (Fig. 7c), (Cel-miR-239b: p < 0.05). Mimetic treatment further resulted in a significant concomitant attenuation of colonic T-bet and IFNγ mRNA levels, indicating that it affects the functional status of CD4+ T cells in vivo (Fig. 7d, e respectively).
To extend our studies into the therapeutic effects of miR-29a mimetics, we next pursued a spontaneous-onset ileitis model using TNF∆ARE/+ mice which is a mouse model of Crohn’s disease (CD) in which CD4+ effector T cells play a central role52. These mice express a single copy of mutated TNFα causing over-stabilization of TNFα mRNA and systemically elevated TNFα protein, eventually leading to a spontaneous development of chronic transmural ileitis resembling CD in humans. Mimetic treatment of TNF∆ARE/+ mice consisted of four intravenous injections during active disease period (Fig. 7f). Upon conclusion of treatment with miR-29a mimetic, TNF∆ARE/+ mice were showing reduced number of IFNγ+ CD4+ T cells in the MLN as compared to control-mimetic treated mice (Fig. 7g), and these animals developed significantly diminished histological changes in the ileum (Fig. 7i, j). These results establish miR-29a as a potential therapeutic agent in the treatment of IBD that may present a viable clinical option in human patients in the future.
Collectively, these experiments demonstrate that liposome-packaged miR-29a mimetic is retained by CD4+ T cells in colitis which may lead to a significant reduction of disease outcomes in multiple IBD models. While we cannot exclude the miR-29a mimetic exerting activity in other cell types during our treatment, miR-29a appears to function as a bona fide regulator of TH1 CD4+ T cell activity during experimental colitis and is a potential therapeutic target in the treatment of IBD.
Discussion
In the present study, we demonstrate that HIF signaling via miR-29a in TH1 T cells is essential for limiting inflammation during colitis. Dysregulated activation and excessive cytokine production by lymphocytes, in particular by CD4+ T cells, has been implicated in driving aberrant inflammation observed during IBD. The molecular machinery coordinating the CD4+ T cell function in IBD is not yet fully understood. Our findings highlight a critical and previously undefined role for HIF-2α in regulating the inflammatory environment within the intestine. We show that inflammatory hypoxia resulting from colitis and its resultant HIF-2α signaling coordinates increased expression of miR-29a in the T cells. This HIF-2α enhancement of miR-29a in turn, represses select TH1 responses including T-bet and IFNγ in CD4+ T cells. Mice with a T cell deficiency in HIF-2α display heightened experimental colitis scores and enhanced TH1 markers in T cells. Mice with a T cell intrinsic deficiency of miR-29a show enhanced colitic profiles in TH1-mediated colitis with concomitantly increased T-bet expression and cytokine production combined with enhanced mucosal injury score. Enhanced TH1 phenotype of miR-29a-deficient T cells leads to impaired TH17 differentiation in the inflamed mucosa in the transfer colitis model. Furthermore, we demonstrate that nanoparticle delivery of miR-29a mimetics can repress bowel inflammation and attenuate TH1 T cell activity in disease.
Recent studies highlighted a decline in oxygen availability during periods of inflammation within the intestinal mucosa, which is a result of increased oxygen consumption by resident mucosal cells and the infiltrating leukocytes. Oxygen deprivation leads to cellular responses that help in adaptation involving activation of hypoxia-inducible factors HIF-1α and HIF-2α in a cell type-specific manner. The importance of HIF signaling during intestinal and systemic inflammation has been studied in the context of epithelial cells and T cells3,53,54,55,56. Similarly, previous studies have addressed the essential role of microRNAs in cellular programs aimed towards resolution of tissue inflammation57,58,59. Our work adds to these findings by demonstrating that T cell-specific HIF-2α mediated miR-29a enhancement is important to limit lymphocyte activation in colitis. The exact function of HIF-2α in colitis appears to be strictly cell-specific as earlier reports show that deletion of HIF-2α in the intestinal epithelium is protective in colitis and prevents colon cancer initiation60,61. Our data points to a disease-protective effect of HIF-2α signaling in CD4+ T cells which are reprogrammed by the hypoxic environment of inflamed intestine to limit their proliferation and cytokine production, therefore limiting their pathogenicity. Understanding the molecular mechanism(s) of hypoxia-driven anti-inflammatory and tissue-protective transcriptional machinery in the infiltrating leukocyte transcriptome is an attractive avenue to target therapeutically.
HIF-1α has been shown to affect the differentiation and function of different T cell subsets under hypoxic and normoxic conditions62. Deletion of Hif-1α in T cells leads to an anti-inflammatory phenotype with increased production of Treg cells and enhanced animal survival in several inflammatory models in vivo, while negatively impacting Treg differentiation and TH1-mediated IFNγ release under hypoxia in vitro3,63. In contrast, the functional role of HIF-2α in the differentiation and activation of T cells is poorly defined. HIF-2α was shown to regulate differentiation of TH9 and Treg cells in a microRNA-dependent pathway20. In addition, a recent report highlighted an unexpected anti-inflammatory role for HIF-2α in Treg stability: Hif-2α-deficient Treg cells have an impaired ability to suppress effector T cells in mouse inflammatory models while making same animals simultaneously resistant to tumor growth18. To the best of our knowledge, our study is the first to directly address the role of HIF-2α in TH1 T cells.
Hundreds of different conserved miRNAs have been identified, however, the role of microRNAs in mucosal immunity and IBD is only beginning to be elucidated64. In T cells, the microRNA expression patterns vary among subsets and stages of cellular development65,66. Of note, deletion of Dicer specifically in murine T cells results in more IFNγ+ CD4+ T cells28 and deletion of Drosha throughout the T cell compartment results in spontaneous inflammatory disease due to the presence of more IL-17A+ CD4+ T cells and/or IFNγ+ CD4+ T cells67. Collectively, these studies suggest that distinct miRNAs play an essential role in the maintenance of T cell immune homeostasis and immune defense29,68. Chief among these, miR-29a, is emerging as a crucial regulator of the adaptive immune system and specifically of the TH1 CD4+ T cells31,45. Global genetic deletion of miR-29a leads to a complex mouse phenotype that manifests with a hematopoietic stem cell deficiency and exhaustion as well as premature attrition of the thymus25,69. By acting on T‐box binding transcription factor (T-bet) and the IFNγ pathway, miR‐29a can suppress the TH1-helper T cell differentiation pathway, and the deletion of miR-29b leads to impaired TH1 responses in the mouse model of multiple sclerosis31,33. Reducing miR-29a expression using a sponge target during ongoing bacterial infection decreases bacterial burden by enhancing the TH1 response in mice. Suppressive effect of miR-29a on IFNγ within the thymic epithelium increases the threshold for infection-associated signals and protects against thymic involution70. Several signal transduction pathways regulating miR-29 family of miRNAs have been described in the context of specific diseases71. For example, a report by Chandiran et al. highlighted the role of Notch-1-mediated miR-29a repression in early T-cell differentiation towards TH172, while others studied the role of AKT-MYC-dependent regulation of miR-29s expression in B cell malignancies33,73, underscoring the cell type-specific regulation of these miRNAs in lymphocyte biology. While our work focuses specifically on the aspects of miR-29a activation in CD4+T cells by tissue hypoxia, some of the previously identified mechanisms of miR-29a activation may also contribute to the phenotypes observed in our study.
In this report we address a major outstanding knowledge gap: how the expression of the microRNA-29a is regulated in the T cells as a reflection of the physiological conditions in the body during homeostasis and under inflammation. We provide relevant biological context for the hypoxia-mediated coordination of miR-29a activity by hypoxia inducible factor 2a (HIF-2α) in T cells within an inflammatory setting. The inflammatory hypoxia within the diseased intestine initiates tissue-protective response that limits immune hyperactivity. Evidence from several studies suggests that pharmacological activation of HIFs may be protective in intestinal inflammation74,75,76,77,78 and potentially some of that protective effect is mediated by HIF-dependent micro RNAs. Our genetic deletion data implicates HIF-2α as an enhancer of miR-29a in T cells during intestinal inflammation, while the miR-29a mimetic significantly ameliorates the diseases outcomes. Indeed, a recent study of acute DSS-induced colitis in mice showed that delivering a miR-29a mimetic within the supercarbonate-apatite complexes may alleviate the intestinal pathology in this model, albeit by targeting distinct components of the innate immune response79 and suggesting additional protective roles for this microRNA. Thus, pharmacologic delivery of miR-29a or stabilization of miR-29a-inducing stimuli (e.g.: T cell-intrinsic HIF-2α) holds promise as a future therapeutic modality for active flares in IBD and a physiologically tailored therapeutic approach to IBD therapies; a welcome alternative to the current broad immune suppressant treatments. The next goal of such deliberate therapeutic interventions will be to improve the delivery routes to specific cellular subsets and to limit non-specific effects of microRNA delivery in the treatment of IBD.
Methods
Mice
C57BL/6J/6J mice were bred in house. HIF-luciferase reporter mice (FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J, Strain #:006206) [referred to as ODD-luciferase], Lck Cre [B6.Cg-Tg(Lck-cre)548Jxm/J, Strain #:003802], Hif-1aloxP/loxP(B6.129-Hif1atm3Rsjo/J, Strain #:007561), Hif-2αloxP/loxP(Epas1tm1Mcs/J, Strain #:008407), LSL-Hif-2dPA (Gt(ROSA)26Sortm4(HIF-2α*)Kael/J, Strain #:009674) and B6.RAG1-/- (B6.129S7-Rag1tm1Mom/J, Strain #:002216) were purchased from Jackson Laboratories (Bar Harbor, ME). Floxed miR-29ab1 (miR-29aloxP/loxP) mice were a gift from Caroline C. Whitacre33 and these were subsequently backcrossed to C57BL/6 J/6 J. T cell intrinsic deficient mice were generated by the F2 progeny of the above floxed strains and Lck Cre+. To eliminate strain or microbiota bias, experiments were performed in littermates using Cre- mice as controls and also in Lck Cre+ control mice. Experimental mice used were age- and sex-matched mice and typically between 8 and 12 weeks of age. To generate a sub-strain of ODD-luciferase mice on a C57BL/6J6/J background, FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J were backcrossed with C57BL/6J6/J mice for over seven generations. The B6.129S-Tnftm2GKl/Jarn strain (TNFΔARE/+; MGI:3720980) was generated by continuous backcrosses between heterozygous TNFΔARE/+ on a mixed background12 to C57BL/6J6/J mice and kept under specific pathogen-free conditions. Experimental animals were heterozygous for the ΔARE mutation (TNFΔARE/+) or homozygous wildtype (WT), which served as controls. In this model mice were used between 10-14 weeks of age. All animals were housed under a 12-hour light/12-hour dark cycle in an animal facility under specific pathogen-free conditions. All mice were handled and euthanized in a humane manner (euthanasia by overdose injection with pentobarbital). Animal procedures were approved by the Institutional Animal Care and Use Committee at the UT Health Science Center in Houston and the University of Colorado, Denver. In conducting research using animals, the investigators adhere to the laws of the United States and regulations of the Department of Agriculture.
Induction of experimental colitis by TNBS
To induced TNBS-colitis, epicutaneous skin sensitization with the hapten, tri-nitro-benzosulfonic acid (TNBS) (1% v/v; EtOH; Sigma Chemical) was performed under anesthesia on experimental day -7 in 8-10 weeks old mice (C57BL/6 J/6 J, Lck Cre+, Hif-2αloxP/loxP Lck Cre +, miR-29aloxP/loxP Lck Cre+, Cre- littermates or LSL-HIF2dPA Lck Cre+). On experimental day 0, anesthetized mice received challenge of intra-rectal TNBS (5ul/g; 2.5% v/v; 40% EtOH), using a 20-gauge soft tygon plastic catheter. Vehicle control animals received a corresponding volume of 40% (v/v) ethanol alone. Body weight loss and disease activity indices (DAI’s) were assessed daily. Control mice for each group included Cre- littermates or in separate experiments Lck Cre+ control mice and no significant experimental differences between these two types of controls was noted.
Induction of chronic colitis by DSS
Mice were given Dextran Sodium Sulfate Salt (40,000 Sigma-Aldrich) in drinking water at 3% for 6 days, followed by two weeks of drinking water. 6-day DSS course was then repeated to induce chronic colitis. Body weight, occult and disease activity were assessed daily.
Induction of experimental colitis by adoptive transfer
B6.RAG1-/- (129S7(B6)-Rag1tm1Mom/J were obtained from the Jackson laboratory and maintained by inhouse breeding in autoclaved housing. 6-8 weeks old male mice were transferred to standard housing and used as recipients of adoptive T cell transfer. Naïve CD4+ T cells were purified with magnetic beads, labeled with fluorescent antibodies against CD4 and CD45RB (both from
Body weight and disease scores were recorded three times per week.
Human subjects
All human studies were approved by the institutional review board of the University of Colorado Denver. CD and UC patients were diagnosed using established criteria and CD phenotype per the Montreal criteria80 (see Supplementary Table 1 for patient characteristics). Patient medications and demographics were recorded at the time of endoscopic evaluation, during which intestinal mucosal biopsies were obtained. Disease activity was determined based upon endoscopic evidence of mucosal inflammation. Informed, written consent was obtained from all patients prior to inclusion in the study. All data was de-identified before sample analysis and only patient age, disease duration and current IBD medication usage were reported to the researchers performing the sample analysis.
Luciferase imaging of T cell-intrinsic HIF activity in vivo
At time of euthanasia, mice were injected intraperitoneally with 300 mg/kg body weight D-luciferin (Calliper or Invitrogen, cat# L2916). Colons and small intestines were used for bioluminescence imaging using the IVIS Imaging System 50 Series (Calliper Life Sciences/Xenogen Corp.). Quantification was performed using Living Image v4.7.2 software. After imaging, mouse tissues were fixed with 4% paraformaldehyde (PFA) solution and paraffin-embedded for analysis of morphology and immunohistochemistry.
Measurement of cellular hypoxia by EF5-flow cytometry
Localization and quantitation of 2-nitroimidazole EF5 binding in hypoxic tissue was used to allow the assessment of hypoxia in the infiltrating T cells during colitis. Colitic mice described in Fig. 1a, b received a 200ul intraperitoneal injection of EF5 (Millipore Sigma) at 30 mg/kg 3 h prior to euthanasia and tissue harvest. EF5 binding was assessed in isolated CD4+ T cell subsets with or without experimental colitis via flow cytometry with an anti-EF5-Cy5 conjugate (5.625ug; clone ELK3-51, Millipore EF5012). A matched isotype antibody (IgG1 CY5, Clone: 15H6, Southern Biotech) was utilized as a negative staining control. Cells were stained and gated on: singlets, live, MHCII-, CD3+, CD4+ and effector memory T cells were additionally gated on CD44+ and CD62L-. Antibodies used were as follows: anti-CD4-FITC (Clone: GK1.5, Cat#: 11-0041-82, Invitrogen), 1:400, anti-CD45RB-PE (Clone: C363.16A, Cat#: 12-0455-82, Invitrogen), 1:600, anti-CD4-eFluor450 (Clone: GK1.5, Cat#: 48-0041-82, Invitrogen), 1:400, anti-CD8a-PE (Clone: 53-6.7, Cat#: 100707, BioLegend), 1:400, anti-CD8a-Pacific Blue (Clone: 53-6.7, Cat#: 100728, BioLegend), 1:400, anti-CD62L-APC (Clone: MEL-14, Cat#: 17-0621-82, Invitrogen), 1:500, anti-CD44-FITC (Clone: IM7, Cat#: 11-0441-82, Invitrogen), 1:200.
Cell isolation
Splenocytes, MLN, and colonic lamina propria mononuclear cells were isolated following experimental colitis experiments as previously described81. Intestinal epithelial cells and colonic lamina propria mononuclear cells in Figs. 2, 5e, 5f and Fig. 6 were prepared using Lamina Propria Dissociation Kit from Miltenyi Biotec (Cat# 130 097 410) per the manufacturer’s protocol and subjected to Percoll gradient (Percoll P4937, Sigma-Aldrich) prior to restimulation and flow cytometry analysis.
Histology
Colons were excised, opened longitudinally and washed with cold PBS followed subsequently by 10% buffered formalin fixation. Tissue was then paraffin-embedded, cut into 5μm sections and stained with hematoxylin/eosin. Histological assessment of colitis sores was performed in a blinded fashion, as previously described8.
Primary T cell isolation
T cells were purified from the spleens and peripheral lymph nodes of mice, mechanically disrupted into a single cell suspension, and subjected to magnetic bead enrichment for CD4+ T cells, using either the CD4+ T cell isolation kit (130-104-453, Miltenyi Biotec), Dynabeads™ Untouched™ Mouse CD4 Cells Kit (11415D, Invitrogen) or EasySep™ Mouse CD4 +T Cell Isolation Kit (19852, StemCell).
Enriched CD4+ T cells were then subjected to FACS purification on a FACS Aria II (BD), with naïve CD4+ T cells purified on the basis of the cell surface phenotype CD4+ CD45RbhighCD62LhighCD25negCD44neg in Fig. 1a, b, or with naïve T cells selected by CD4posCD45RBhighCD25neg or CD4posCD45RBlow(control) on a FACS Aria II (BD) in all subsequent adoptive transfer experiments. Antibodies used were as follows: anti-CD62L-APC (Clone: MEL-14, Cat#: 17-0621-82, Invitrogen), 1:500, anti-CD44-FITC (Clone: IM7, Cat#: 11-0441-82, Invitrogen), 1:200, anti-CD25-PB (Clone PC61.5 Cat#: 404-0251-82, Invitrogen), 1:400, anti-CD4-FITC (Clone: GK1.5, Cat#: 11-0041-82, Invitrogen), 1:400, anti-CD45RB-PE (Clone: C363.16A, Cat#: 12-0455-82, Invitrogen), 1:600, anti-CD62L-APC (Clone: MEL-14, Cat#: 17-0621-82, Invitrogen), 1:500.
In vitro T cell culture
Purified primary mouse and human CD4+ T cells were cultured (1×106) cells/ml in RPMI-1640 (Gibco) containing FBS (5% v/v), L-glutamine (1% v/v), Antibiotic Antimycotic Solution at 1X (A5955, Sigma Aldrich), and β-mercaptoethanol (50 μM). CD4+ T cells were stimulated with plate-bound anti-CD3ε (1 μg; clone 145-2C11; Cat#: 100359, BioLegend), soluble anti-CD28 (2 μg/ml; Clone: 37.51, Cat#: 102121, BioLegend) and supplemented with 10 ng/mL of recombinant mouse IL-2 (eBioscience). For differentiation of CD4+ helper lineages, naïve CD4+ CD25negCD62L+ T cells were harvested from spleen of 6–10 weeks old mice by magnetic bead enrichment and cultured ex vivo for 3-5 days in lineage-polarizing conditions. Briefly, TH0 T cells were maintained with the addition of recombinant IL-2 (200 U/ml; Peprotech), anti-IL-12 (C17.8, Catalog #BE0051, BioXCell) 10 μg/ml, anti-IFNγ (Clone XMG1.2, Catalog #BE0055, BioXCell) 10 μg/ml and anti-IL-4 (11B11, Catalog #BE0045, 10 μg/ml; BioXCell). TH1 differentiation was achieved with the addition of recombinant IL-2 (10 ng/ml; Peprotech) and anti-IL-4 (10 μg/ml; BioXCell). TH2 were differentiated with the addition of recombinant IL-4 (10 ng/ml; RnD systems) and anti-IFNγ (10 μg/ml; BioXCell). TH17 were differentiated with recombinant TGFβ (5 ng/ml; RnD systems), IL-23 (10 ng/ml, RnD systems), IL-6 (20 ng/ml; RnD systems), anti-IL-4 (10 μg/ml; BioXCell) and anti-IFNγ (10 μg/ml; BioXCell). Lastly, Treg cells were differentiated with the addition of recombinant TGFβ (15 ng/ml, RnD systems), IL-2 (200 U/ml, Peprotech), anti-IL-12, anti-IFNγ and anti-IL-4 (10 μg/ml; BioXCell). In a subset of experiment, pharmacologic stabilization of HIF with the PHD inhibitor, Dimethyloxallyl Glycine (DMOG; Calbiochem) was used under conditions of normoxia (21% O2) or hypoxia (1% O2). In Fig. 6 and Supplementary Fig. 4 and 5, T cells were differentiated exactly as above but the antibodies used for differentiation conditions were as follows: anti-IFNγ (Clone: XMG1.2, Cat#: 505847, BioLegend), 10ug/ml anti-IL-4 (Clone: 11B11, Cat#: 504136, BioLegend), 5ug/ml, anti-IL-2 (Clone: JES6-1A12, Cat#: 503707, BioLegend), 5ug/ml.
T cell culture and transfection with miRNAs
TH1 skewed (day 3) CD4+ T cells were rested overnight in fresh medium. Subsequently, the T cells were transfected with 100 nM miRNA mimetic or 300 nM miRNA inhibitor (both from Dharmacon, Lafayette, CO) using Lipofectamine 3000 (Invitrogen), following the manufacturer’s recommendations. Twelve hours after transfection cells were re-stimulated with 5.0 µg/ml anti-CD3/anti-CD28 mAbs (Cat#: 100359, 102121, BioLegend) and collected for further analysis 48 h later.
microRNA and mRNA transcriptional analysis
Total RNA or separated fractions of miRNA and mRNA were isolated from in vitro differentiated T cells or whole colon tissues using QIAzol Reagent (Cat. # 79306) and RNeasy kit (Cat.# 74104) following manufacturer instructions (Qiagen). Transcript levels were determined by real-time RT-PCR using specific primer sets (Qiagen and ThermoFisher) with Bio-Rad CFX384 system. Expression levels of miRNA’s were normalized to endogenous RNU-6, while 18 s was used to normalize mRNA expression profiles. Limited target q-PCR based array with miRNA-specific primers from Qiagen (custom array) and Bio-Rad CFX384 system was used to measure changes in T cell-specific microRNAs shown in Fig. 2a; data was then normalized to the expression of RNU6 and further compared to the average of normoxia samples to create the heatmap. RNA for the array was prepared from T cells stimulated with anti-CD3/anti-CD28 mAbs (Cat#: 100359, 102121, BioLegend) for 8 h in normoxia and hypoxia.
Flow cytometry
Cells from indicated in vivo compartments or cell culture experiments, or cells prepared for FACS sorting were incubated with fluorescently labeled anti-mouse antibodies against: anti-CD4-FITC (Clone: GK1.5, Cat#: 11-0041-82, Invitrogen), 1:400, anti-CD8a-PE (Clone: 53-6.7, Cat#: 100707, BioLegend), 1:400, anti-CD62L-APC (Clone: MEL-14, Cat#: 17-0621-82, Invitrogen), 1:500, anti-CD44-FITC (Clone: IM7, Cat#: 11-0441-82, Invitrogen), 1:200, anti-IFNγ-APC (Clone: XMG1.2, Cat#: MABF1515, Sigma-Aldrich), 1:400, anti-IL-17a-PE (Clone:TC11-18H10.1, Cat#: 506903, BioLegend), 1:400, anti-TNFα-PE (Clone: TN3-19.12, Cat#: 506104, BioLegend), 1:400, anti-T-bet-PE (Clone: O4-46, Cat#: 561268, BD Bioscience), 1:400, anti-T-bet-BV421 (Clone: O4-46, Cat#: 563318, BD Bioscience), 1:400, anti-CD45RB-PE (Clone: C363.16A, Cat#: 12-0455-82, Invitrogen), 1:600 (eBioscience), or corresponding isotype controls. Intracellular staining was performed using the FoxP3 staining kit (eBioscience) according to the manufacturer’s instructions. Intracellular cytokine staining was performed following 4 h of stimulation with PMA, Ionomycin and Brefeldin A. Analysis was performed using a BD FACS Canto™ II (BD Biosciences) or CytoFLEX LX (Beckman Coulter Lifesciences). Cell sorting was performed using a BD FACS Aria II (BD Biosciences). Further data analyses were performed using FLOWJo software (Tree Star Inc, Ashland, OR). Example of gating strategy used for T cells is provided in Supplementary Fig. 7.
Chromatin immuno-precipitation (ChIP) assay
ChIP was performed to assess DNA-protein interactions at the promoter sequences using the ChIP assay kit (Cell Signaling Technologies #9004) according to the manufacturer’s instructions. Briefly, CD4 T cells were purified with magnetic beads and activated with anti-CD3/anti-CD28 mAbs (Cat#: 100359, 102121, BioLegend) under hypoxia for 8 h and subsequently harvested. Protein-DNA complexes were cross-linked by fixing with 1% (v/v) formaldehyde in a culture medium for 10 min. Cross-linking was stopped by 5 min of incubation with 125 mM glycine and nuclear contents were subsequently extracted. Chromatin was digested with Micrococcal Nuclease (MNase, provided from the kit) to yield fragments between 150–300 bp and the DNA was then immunoprecipitated overnight at 4 °C with 2 μg antibody specific to HIF2A (NB100-122, Novus) or control isotype Rabbit IgG (#2729, Cell Signaling Technologies). The DNA-antibody complexes were pull-down with agarose beads. The complexes were eluted from the beads and cross-linking was reversed by incubation for 2.5 h at 65 °C with proteinase K. Reaction was then stopped, and DNA products were analyzed by real-time PCR using the following primer pairs: HRE1- For GCGATCCTATGCTGACCTCA, HRE1- Rev CTGTCCCACGTCTGCTCTC, HRE2-For GAGCTCAGCACACTTTTGATAAG, HRE2-Rev TGTGTTGCTTTGCCTTTGAGA, HRE3-For CCCTTTATCCCTTCCTGCTCAT, HRE3-Rev ATGGACTAAGCCGGCTGTAG. Only 3 proximal HREs were assayed and the remaining 4 distant putative HREs were not assessed for binding.
In vivo nanoparticle delivery of mmu-miR-29a mimetic
To utilize a pharmacological approach to overexpress mmu-miR-29a during experimental TNBS-colitis, a nanoliposomal-based approach was employed to transport synthetic mmu-miR-29a. DOPC consists of 1,2-dioleoyl-sn-glycero-3-phosphocholine, squalene oil, polysorbate 20, and an antioxidant which when in complex with synthetic miRNAs can form nanoparticles in the nanometer diameter82 range. Packaging of the microRNA mimics was performed as described previously83. Mice were treated on indicated days during the course of colitis or ileitis with 50 µg synthetic mmu-miR-29a mimic (Dharmacon, Lafayette, CO) or respective control (mimic-ctr) formulated with LNP by intravenous retro-orbital injection.
Statistical analysis
Mice were randomized into experimental groups, and both male and female mice were used in all studies with the exception of transfer colitis where only male mice were used as recipients of the adoptive transfers. All experimental, disease and histological scoring was performed by researchers blinded to the identity of the samples.
Statistical analyses were performed using GraphPad Prism 8 software, and t test and one-way ANOVA or two-way ANOVA tests were used as indicated, respectively. Data were expressed as mean ± standard error of the mean (S.E.M). Statistical significance was set at p < 0.05.
Diagrams in Fig. 7 were prepared with BioRender.com
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
Source Data file containing all data points is provided with this manuscript. Data set that includes reads from the limited T-cell focused microRNA PCR-based microarray from Qiagen (first generation) is provided as Supplementary Data 1 (titled T-cell microarray). Any additional requests for information can be directed to, and will be fulfilled by, the corresponding authors. Source data are provided with this paper.
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Acknowledgements
The authors would like to thank the members of the Eltzschig lab, Kelley Brodsky and Eric T. Clambey for technical assistance with experimentation. We thank Frank Xuebo Chen for expert technical assistance with animal husbandry and breeding. Martine McManus performed the TNBS colitis scoring in Figs. 4, 5j and 7c. This work was supported by The Assistant Secretary of Defense for Health Affairs endorsed by the Department of Defense, in the amount of ($312,000), through the Peer Reviewed Medical Research Program under Award Number (HT9425-23-1-0094) to A.K.C. Opinions, interpretations, conclusions, and recommendations are those of the authors and are not necessarily endorsed by The Assistant Secretary of Defense for Health Affairs endorsed by the Department of Defense. The work was also supported by the National Institute of Health Grants R01-HL154720, R01-DK122796, R01-HL133900 and by a grant from the Crohn’s and Colitis Foundation of America (CCFA) to H.K.E.; Crohn’s and Colitis Foundation of America grants (CCFA #273007, #409992) and National Institute of Diabetes and Digestive and Kidney Diseases (# R01DK111856) to E.McN and E.DeZ. W.R. is supported by International Anesthesia Research Society (IARS) Mentored Research Award. X.Y. is supported by National Institute of Health Grants R01HL155950, Parker B. Francis Fellowship, and American Lung Association Catalyst Award CA-622265.
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Conceptualization, H.K.E., A.K.C., and E.McN; Methodology; A.K.C., E.McN., E.T.C, J.C.M., C.B.C., H.K.E.; Investigation, A.K.C., E.McN., E.DeZ, X.H., V.V, T.C., I.B, J.C.M., E.T., C.B.C., S.F., H.L., T.N; Writing–Original Draft, A.K.C, E.McN., and H.K.E.; Writing–Review & Editing, A.K.C., E.McN, Y.W, X.H., W.R, E.DeZ, H.K.E.; Resources, A.S., M.McM., M.E.G., G.T.F, S.P.C., X.Y, C.R-A., G.L-B., H.K.E.; Supervision & Project administration, A.K.C., E.McN., and H.K.E.
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Czopik, A.K., McNamee, E.N., Vaughn, V. et al. HIF-2α-dependent induction of miR-29a restrains TH1 activity during T cell dependent colitis. Nat Commun 15, 8042 (2024). https://doi.org/10.1038/s41467-024-52113-y
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DOI: https://doi.org/10.1038/s41467-024-52113-y
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