Aryl amino acetamides prevent Plasmodium falciparum ring development via targeting the lipid-transfer protein PfSTART1

With resistance to most antimalarials increasing, it is imperative that new drugs are developed. We previously identified an aryl acetamide compound, MMV006833 (M-833), that inhibited the ring-stage development of newly invaded merozoites. Here, we select parasites resistant to M-833 and identify mutations in the START lipid transfer protein (PF3D7_0104200, PfSTART1). Introducing PfSTART1 mutations into wildtype parasites reproduces resistance to M-833 as well as to more potent analogues. PfSTART1 binding to the analogues is validated using organic solvent-based Proteome Integral Solubility Alteration (Solvent PISA) assays. Imaging of invading merozoites shows the inhibitors prevent the development of ring-stage parasites potentially by inhibiting the expansion of the encasing parasitophorous vacuole membrane. The PfSTART1-targeting compounds also block transmission to mosquitoes and with multiple stages of the parasite’s lifecycle being affected, PfSTART1 represents a drug target with a new mechanism of action.

With resistance to most antimalarials increasing, it is imperative that new drugs are developed.We previously identified an aryl acetamide compound, MMV006833 (M-833), that inhibited the ring-stage development of newly invaded merozoites.Here, we select parasites resistant to M-833 and identify mutations in the START lipid transfer protein (PF3D7_0104200, PfSTART1).Introducing PfSTART1 mutations into wildtype parasites reproduces resistance to M-833 as well as to more potent analogues.PfSTART1 binding to the analogues is validated using organic solvent-based Proteome Integral Solubility Alteration (Solvent PISA) assays.Imaging of invading merozoites shows the inhibitors prevent the development of ring-stage parasites potentially by inhibiting the expansion of the encasing parasitophorous vacuole membrane.The PfSTART1-targeting compounds also block transmission to mosquitoes and with multiple stages of the parasite's lifecycle being affected, PfSTART1 represents a drug target with a new mechanism of action.
There were an estimated 249 million cases of malaria in 2022 resulting in approximately 608,000 deaths, mainly in sub-Saharan Africa 1 .Artemisinin combination therapies (ACTs) remain the frontline treatments for malaria infections by eliminating the causative Plasmodium parasites from patients.Of concern is that mutations in the parasite's kelch13 gene, a marker of partial resistance to ACTs, have now emerged in many regions around the world and portents to increasing treatment failures [2][3][4][5][6] .For this reason, new small molecule inhibitors with novel mechanisms of action that can kill various lifecycle stages of the parasite need to enter the developmental pipeline.
A prime process to target is invasion by the free-form merozoite into host red blood cells (RBCs) 7 .This process occurs rapidly in less than a minute 8,9 and requires many protein-protein interactions to successfully occur in a precise, carefully choreographed order 10 .To identify inhibitors with novel mechanisms of action against RBC invasion, we previously performed a phenotypic screen of the 400 compound Medicines for Malaria Venture (MMV) Pathogen Box 11 .One hit compound, MMV006833 (M-833), had a novel effect whereby the merozoite entered its target RBC but failed to differentiate into an amoeboid ring-stage parasite during the formation of the parasitophorous vacuole membrane (PVM) around the parasite 11 .Here, we generated M-833 resistant parasites and whole genome sequencing revealed mutations in the Plasmodium falciparum Steroidogenic Acute Regulatory protein-related lipid Transfer (START) domain-containing phospholipid transfer protein (PF3D7_0104200, PFA0210c, also called PV6 12 ).
START-domain proteins can ferry lipids between membranes and are defined by their START region of ~210 amino acids that form a hydrophobic lipid-binding pocket.START domains typically comprise an α/β helix-grip fold with antiparallel β-sheets, flanked by N-and C-terminal α-helices 13 .The human START domain family encompasses 15 members 14 , whilst Plasmodium spp. in contrast has only five known START-domain proteins 15 .Of those five, PFA0210c is the most studied and the focus of this article, which we will refer to as PfSTART1.PfSTART1 shares sequence similarities of 20% with the human StarD7, which is a phosphatidylcholine transfer protein involved in maintaining mitochondrial membrane integrity.However, PfSTART1 is structurally closer to human StarD2, another phosphatidylcholine transfer protein involved in lipid droplet metabolism 15,16 .In parasites, PfSTART1 is essential during the blood stages and most strongly expressed during schizogony 16 .PfSTART1 can transfer a wide range of phospholipids in vitro, with a preference for phosphatidylcholine and phosphatidylinositol 16 which is regulated by an unusual C-terminal extension that is essential for growth in vivo 17 .Due to its function and expression profile, previous studies have suggested that PfSTART1 may play a role in forming the PVM upon merozoite invasion by transferring lipids from the RBC or parasite membranes into the nascent PVM, and therefore represents a promising drug target 16,17 .
Here, we report the M-833 series as inhibitors of PfSTART1.In this study, we identify mutations in the START domain of PfSTART1 in parasites resistant to M-833.The introduction of these mutations into 3D7 wildtype parasites conferred resistance to M-833 and its highly potent analogues.We further investigate how the M-833 series binds to PfSTART1 and how this blocks merozoite development into ring-stage parasites.

Results
Selection for resistance to MMV006833 followed by whole genome sequencing identified PfSTART1 as a possible target To select for resistance to MMV006833 (Fig. 1A) hereafter called M-833, five populations (A-E) of 10 8 asexual blood stage 3D7 parasites were cycled on and off 3 µM of M-833 (corresponding to 10 x EC 50 ).After three cycles of drug treatment, three parasite populations were identified as less susceptible to the compound and their growth in serially diluted M-833 after 72 h was measured by lactate dehydrogenase (LDH) activity assays.The EC 50 of the drug-treated parasites was 2.18 to >10-fold higher than 3D7 wild-type parasites, demonstrating resistance had been generated (Fig. S1A).The two most resistant populations (PopD and PopE) were further cloned out by limiting dilution.Growth assays demonstrated the resistance against M-833 was heritable, with a 17-fold increase in EC 50 compared to 3D7 parasites (Fig. 1B).To identify the target of M-833, genomic DNA was extracted from the clonal lines of PopD and PopE and subjected to whole genome sequencing using MinION technology and compared to clonal 3D7 parental parasites 18 .This identified 14 non-synonymous single nucleotide polymorphisms (SNP) across nine different genes when compared to the 3D7 parental strain (Supplementary Data 1).The two genes that had SNPs present across all resistant clones were the P. falciparum liver stage antigen 1 gene (PF3D7_1036400) and P. falciparum stAR-related lipid transfer protein (PF3D7_0104200, PFA0210c; pfstart1).The former is not expressed in the blood stage [19][20][21] and comprises a large central repeat region which is problematic for variant calling.Therefore, we focused on pfstart1: two of the three sequenced PopD clones (PopD-C6 and -F7) had a SNP (AAC-AAG) which caused a mutation N309K in PfSTART1 (Fig. 1C, Supplementary Data 1).The third PopD clone (PopD-E3) also contained the N309K mutation, although this had been removed during quality filtration as it did not meet the minimum depth of 10x coverage.While we did not sequence the genome of PopD-D7, it also contained the N309K mutation, as demonstrated by PCR amplification and sequencing (Fig. S1B).Both PopE clone genomes had a SNP (AAC-AAA) within pfstart1 that resulted in a mutation N330K (Fig. 1C, Supplementary Data 1).Finally, PCR-amplification and sequencing of pfstart1 of PopC showed it contained an I224F mutation (Fig. S1C).Collectively, all the identified mutations (N309K, I224F, N330K) were found within the central conserved START domain and predicted to face the lipid-binding pocket (Fig. 1C).

Introduction of N309K and N330K mutations into pfstart1 gene increases resistance to M-833
To test whether PfSTART1 was the target of the M-833 series, the N309K and N330K mutations were first introduced into the pfstart1 gene using selection-linked integration (SLI) that integrates via a single crossover homologous recombination 22 .This construct contained a haemagglutinin (HA) tag at the C-terminus and a glmS riboswitch to allow protein knock-down after addition of glucosamine (GlcN) which was separated by a P2A-skip peptide and neomycin resistance gene (Fig. 2A).After clonal lines of the WT, N309K and N330K had been obtained, integration was confirmed by PCR (Fig. S2A), and the modified pfstart1 loci were sequenced to ensure the mutations were correct (Fig. S2B).The clonal parasites obtained with the SLI method are hereafter called SLI-WT, SLI-N309K, and SLI-N330K.We challenged SLI-WT, SLI-N309K and SLI-N330K with M-833 to determine the effect of the mutations on M-833 treatment (Fig. 2B).SLI-N309K and SLI-N330K parasites were 10-and 64-fold more resistant than the 3D7 parental parasites respectively.Unexpectedly, we found that the SLI-WT parasites, in which the recodonised WT pfstart1 sequence was used to replace endogenous coding sequence, were 7-fold more sensitive to M-833 compared to the parental 3D7 parasites.
Since the addition of the HA tag and P2A skip peptide could interfere with PfSTART1 function and sensitivity of SLI-WT parasites to M-833 a different tagging strategy was used, whereby the introduction of the mutations into pfstart1 did not include HA and P2A, using CRISPR-Cas9 23 .In comparison to the SLI plasmids, the CRISPR constructs contained an additional 3' homology block designed to enable double crossover and limited plasmid insertion (Fig. 2C).Correct integration was confirmed by PCR (Fig. S2D), and the modified pfstart1 loci were sequenced (Fig. S2E).These CRISPR parasite clones were hereafter called CR-WT, CR-N309K, and CR-N330K.CR-N309K and CR-N330K parasites exhibited resistance to M-833 to a similar extent as the original resistant parasites (over 81-fold resistance compared to CR-WT, Fig. 2D).In addition, the CR-WT parasites displayed an EC 50 against M-833 comparable to 3D7 (51.2 and 49.7 nM respectively).Overall, these results demonstrate that the mutations N309K and N330K in PfSTART1 confer resistance to M-833.

Knockdown of PfSTART1 sensitises parasites to M-833
To examine if knocking down PfSTART1 would decrease the EC 50 of M-833 due to a reduction in target protein levels we tested the knockdown efficiency exerted by the glmS riboswitch integrated downstream of the pfstart1 genes (Fig. 3A, D).Wildtype plasmepsin V processed PfSTART1 is predicted to be about 48 kDa as observed by western blot for 3D7 parasites (Fig. 3A).The 3xHA tag and P2A peptide add 5.8 kDa to the protein in line with that observed for the SLI-tagged parasites (Fig. 3A).In SLI-N309K, there was a smaller band detected that was similar in size to the native protein and contained no HA-tag.To remove any contaminating wildtype parasites the parasites were recloned, but all lines still contained the two PfSTART1 bands.Diagnostic PCRs of the SLI-N309K parasites indicated the native gene was tagged so perhaps the smaller band arose from post-translational processing of the SLI-N309K protein.Western blots of the CRISPR-tagged parasites using the anti-PfSTART1 antibody detected proteins of the expected size (Fig. 3D).
Upon addition of 2.5 mM glucosamine (GlcN) for 48 h PfSTART1 was successfully knocked down in schizonts in both the SLI and the CRISPR parasites (Fig. 3B, E).The knockdown was stronger in the CRISPR constructs (75% for CR-WT, 81% for CR-N309K, 84% for CR-N330K) compared to the SLI parasites (60% for SLI-WT, 43% for SLI-N309K, 70% for SLI-N330K).This was expected as the glmS riboswitch is located further downstream of the pfstart1 coding sequence in the SLI constructs compared to the CRISPR constructs.
To measure the impact of PfSTART1 knockdown on parasite growth, ring-stage parasites (3D7, SLI-and CR-WT, N309K, N330K) were exposed to 0, 0.25, or 2.5 mM GlcN for 72 h, and their growth was assessed by LDH activity (Fig. S2C and F).In the presence of 2.5 mM GlcN, all SLI-and CR-parasites grew significantly less than in the absence of GlcN: approximately 40% less for most lines, and down to 50% less in the case of CR-N309K.3D7 parasites were slightly impacted by the presence of 2.5 mM GlcN, but to a lesser extent, with ~5% reduced growth.The data therefore indicates that the reduction in the expression of PfSTART1 reduces parasite growth.
To determine if the knockdown of PfSTART1 also increased the sensitivity of the parasites to M-833, a growth inhibition assay was performed in the presence of 0, 0.25, or 2.5 mM GlcN, and the EC 50 calculated (Figs 3C, F).In both the SLI-WT and the CR-WT parasites, the addition of 2.5 mM GlcN significantly reduced the EC 50 values: by 2.3fold for more sensitive SLI-WT; 7.8-fold for CR-WT.In the case of the N309K mutation, the M-833 EC 50 values were reduced by similar amounts to the WT parasites in the presence of 2.5 mM GlcN (2.5-fold reduction for SLI-N309K, 5.2-fold reduction for CR-N309K), although this did not reach statistical significance.Regarding the N330K mutants, the addition of GlcN did not impact the EC 50 of M-833 in CR-N330K parasites.As expected, the addition of GlcN did not change the EC 50 of control 3D7 parasites.Together, these results confirm that M-833 targets PfSTART1, and that PfSTART1 is important for parasite growth.

M-833-resistant parasites are also resistant to highly potent analogues
To investigate the therapeutic potential of the compounds, we explored the structure activity relationship of the M-833 series.It was observed that M-833 (1) shared structural similarities with the aryl aminoacetamide compound 2 24 (Fig. 4A), which was developed from a Tres Cantos Antimalarial Set (TCAMS) hit.The structural similarities include a substituted aryl sulfonamide, acetamide core and aryl amido substitution in both series.As the mechanism of action for the Norcross et al. series has not been determined, its similarities to M-833 suggest it may share the same target.Compound 2 was assessed for activity against M-833-resistant populations and was shown to be cross-resistant (Fig. 4B), indicating compound 2 is likely to have the same molecular target as M-833.In the optimisation of M-833 it was found that there was a crossover with the structure-activity relationship between the two structurally related hit compounds 24 .Combining the gem dimethyl and the aryl oxy structural elements from M-833 and compound 2 respectively, and removal of the 2-methyl group from M-833 led to the intermediate hybrid compound 3 that exhibited a 10-fold improvement in antiparasitic activity (EC 50 = 120 nM) relative to the activity of M-833.Replacing the aryl oxy functionality in compound 3 with an amino group led to W-991 (WEHI-991), resulting in a 20-fold improvement in parasite activity (EC 50 = 7 nM) (Fig. 4A).This change also led to a small increase in human HepG2 cell activity (CC 50 = 29 µM).This activity is consistent with that previously reported 24 .Methylation of the amido group resulted in an inactive analogue (compound 4) (EC 50 > 10 µM) which was used as a negative control for our studies (Fig. 4A).
These newly synthesised analogues were tested against the original M-833 resistant parasites PopD-D7 and PopE-F10 which contain the PfSTART1 N309K and N330K mutations respectively.Both parasite lines were highly resistant to the active analogues (compound 2, W-991, and compound 3) (Fig. 4B, Fig. S3 and Table S1).No change in EC 50 was observed between the resistant lines and 3D7 for the inactive analogue 4.
Growth inhibition assays with GlcN were also carried out on the CRISPR parasites, using the potent analogue W-991 (Fig. 4C).The first observation was that the CR-N309K and CR-N330K parasites were around 600-fold less susceptible than the 3D7 and the CR-WT controls.This demonstrates that the PfSTART1 mutations confer resistance to W-991.However, it should be noted that sub-micromolar potency was retained against the mutant parasites with an EC 50 below 700 nM in both cases.When PfSTART1 was knocked down with the addition of GlcN, all CRISPR parasites became significantly sensitised to W-991 (7fold for CR-WT, 5-fold for CR-N309K, 7.3-fold for CR-N330K), while the addition of GlcN did not change the EC 50 on control 3D7 parasites.These results show that we successfully developed potent analogues of M-833, that all appear to target PfSTART1.
M-833-series binds to recombinant PfSTART1 protein and PfSTART1 in parasites, but not to the mutant PfSTART1(N330K) protein To independently confirm that PfSTART1 was the target for the M-833 series, we expressed recombinant wildtype PfSTART1(WT) (I149-V394) and measured binding to M-833, W-991 and negative control compound 4 by isothermal titration calorimetry (ITC) (Fig. 5A, Fig. S4A-C).M-833 bound PfSTART1(WT) with nanomolar affinity (K D = 42 ± 12 nM), and was both enthalpically and entropically favoured, which is an optimal thermodynamic signature for inhibitor/target interactions (Fig. 5A).The optimised analogue, W-991, had improved binding affinity for PfSTART1 (K D = 10 ± 7 nM) compared to M-833.This improved binding affinity can be largely attributed to an enhanced ΔH, suggesting improved hydrogen bond contributions.PfSTART1(WT) did not interact with the chemically related but inactive analogue 4.
To investigate the effect of the resistant mutations to compound binding we also recombinantly expressed PfSTART1(N309K) and PfSTART1(N330K) (I149-V394) (Fig. S4A, B).Since the former had a poor yield, only PfSTART1(N330K) was evaluated in ITC binding experiments.This revealed no interaction with potent analogue W-991 (Fig. 5A, Fig. S4C), indicating the lysine substitute at the 330 position was the mechanism of parasite resistance in PopE parasites.
Next, to assess the specificity of the M-883 series we carried out drug-target engagement profiling in parasites.Here we leveraged the principle of Solvent Induced Protein Precipitation (SIP) which identifies protein-ligand interactions based on differential susceptibility of ligand-bound proteins to denaturation by organic solvents [25][26][27] .Here, soluble parasite protein lysate was exposed to increasing concentrations (0-25%) of a mixture of acetone, ethanol, and formic acid (AEF) in the presence of W-991 or DMSO, followed by soluble protein isolation via centrifugation.Soluble proteins were separated by SDS-PAGE and probed with an PfSTART1 antibody (Fig. 5B).This showed that W-991 elicited protection to PfSTART1 aggregation at 19-21% AEF (Fig. 5B, Fig. S4D).To further investigate the target engagement of W-991, we next turned to a global proteome analysis in the SIP assay.After the solvent challenge, soluble protein fractions were subsequently combined into two samples representing low and high denaturation pressure (7-15% and 17-25% AEF, respectively), following a Proteome Integral Solubility Alteration (PISA) experimental format 28 .Relative protein abundance was subsequently determined through Data Independent Acquisition mass spectrometry (DIA-MS) analysis (2,393 Plasmodium proteins with ≥3 peptides), followed by differential abundance analysis of drug-and vehicle-treated samples.This 'Solvent-PISA' assay revealed four proteins exhibiting significantly (p < 0.01) increased levels in the presence of W-991, of which PfSTART1 was the most significant (Fig. 5C, p = 0.0014).The three other drugstabilised proteins included merozoites-associated armadillo repeats protein (MAAP, PF3D7_1035900), ring-infected erythrocyte surface antigen (RESA3, PF3D7_1149200) and signal recognition particle subunit (SRP19, PF3D7_1216300) (Fig. 5C).The abundance/stability of the other two START-domain containing P. falciparum proteins detected in the assay was not affected by W-991 treatment (Fig. S4E).Taken together, the ITC and Solvent-PISA experiments strongly support PfSTART1 as the principal molecular target of the M-833 series.

PfSTART1 inhibitors block ring development but their effect is reversible
M-833 treated parasites have been observed to invade normally but to stall before ring development 11 .To investigate whether parasites could recover from M-833 and W-991 treatment, parasites were treated similarly as in the Dans et al. study, and then followed-up for 3 days.Late-stage schizonts were treated for 4 h (during the egress/invasion window) with M-833, W-991, ML10 (reversible egress inhibitor), E64 (irreversible egress inhibitor), and heparin (invasion blocker), after which the compounds were removed, non-egressed schizonts eliminated with a sorbitol treatment, and parasite growth and phenotype monitored over 3 days (Fig. 6A, B).Growth was assessed every 24 h via bioluminescence of nanoluciferase (Nluc) in parasites expressing Hyp1-Nluc 29,30 .All treatments significantly reduced parasite growth compared to DMSO (Fig. 6B), with M-833, W-991, and heparin displaying the most striking difference, with virtually no recovery after three days (72 h).A short 4 h treatment was also sufficient to affect the morphology of the treated parasites for days: at 72 h, M-833 and W-991-treated parasites resembled the dysmorphic ring-stage parasites described previously in the genetic knockout of PfSTART1 (Fig. 6A) 11,17 .Based on the phenotype reminiscent of the PfSTART1-knockout and the data shown in the paper, we are hereafter referring to M-833 and W-991 as PfSTART1 inhibitors.
After confirmation that the M-833 series was blocking normal ringstage development, we sought to determine (1) whether other intraerythrocytic stages were affected and (2) whether this resulted in parasite death or stasis beyond 72 h.To do this, we treated highly synchronous ring-stage parasites with DMSO, M-833 or W-991 and followed them through a cycle of growth, which did not indicate any impact on alreadydeveloped rings or trophozoites (Fig. S5A).We did, however, observe a slight developmental delay of schizonts upon compound treatment at 36 h.Despite this delay, the presence of dysmorphic rings in the M-833 and W-991-treatments was confirmed after 48 h (Figs.S5A, 6C).Drugtreated samples were then exposed to sorbitol lysis to remove unruptured schizonts and compounds were washed out.Parasites were then returned to culture alongside their continuously treated counterparts.This revealed that after 6 days post washout (T = 192 h), parasites that had been pre-treated with either M-833 or W-991 were able to resume normal growth, which was visible by both the increase in parasitemia as measured by SYBR Green staining (Fig. 6D) and Giemsa-stained blood smears (Fig. 6C), indicating that one cycle of treatment is not sufficient to cause irreversible inhibition of parasite growth.To corroborate this, we performed parasite reduction ratio (PRR) assays whereby the parasites were exposed to W-991 for up to 5 days before the compound was removed and parasites returned to culture for 21 days.This revealed that despite a reduction of viable parasites after an increasing exposure time to W-991, a proportion of parasites remained after a treatment period of 5 days (Fig. S5B).Taken together, these experiments show that complete inhibition of in vitro parasite growth with the PfSTART1 inhibitors requires >5 days of continuous treatment, and parasite survival with <5 days of treatment is likely due to stasis in the ring-stage of the asexual lifecycle.

PfSTART1 inhibitors block differentiation into ring-stage parasites by preventing PVM expansion
It has been previously postulated that the phospholipid transfer activity of PfSTART1 may be required for the expansion of the parasitophorous vacuole (PV) after invasion 16 .Since previous live cell imaging of merozoites treated with M-833 also showed a defect in ringstage establishment 11 , we utilised lattice light sheet microscopy to visualise PVM formation directly after merozoite invasion in the presence of our potent inhibitor W-991.To track these events, the RBC membrane was labelled with a fluorescent steryl dye, Di-4-ANEPPDHQ, and merozoites with Mitotracker Deep Red dye 31 .Consistent with previous reports 9,31 , several minutes after invasion in the DMSO control, the PVM was observed to become less spherical and more irregularly shaped consistent with the differentiation of the spherical merozoite into an amoeboid ring (Fig. 7A, Movie S1).In contrast, newly invaded merozoites treated with W-991 remained spherical over this period (Fig. 7A, Movie S2).Bioinformatic analysis of 4-dimensional Fig. 3 | Knocking-down PfSTART1 sensitises parasites to M-833.Engineered parasites contain a riboswitch glmS system: when glucosamine (GlcN) is added, PfSTART1 is knocked-down.A 3D7, selection linked integrated (SLI)-wildtype (WT), -N309K or -N330K were exposed for 48 h to 0 or 2.5 mM GlcN, and proteins were extracted from schizonts.Western blots were probed with anti-PfSTART1, anti-HA, and anti-PfEXP2 antibodies.B The densitometry of 3 biological replicates was measured (the PfSTART1 signal was normalised by the corresponding PfEXP2 signal, and then normalised by the 0 mM GlcN condition within each parasite line; mean +/− SD).C EC 50 values of M-833 on 3D7 and SLI-parasites in the presence of 0, 0.25, or 2.5 mM glucosamine (GlcN).n = 4 biological replicates, mean +/− SD.Ordinary one-way ANOVA with Dunnett's multiple comparison test.
* adjusted p value = 0.0334.EC 50 values and 95% confidence intervals are indicated in the table.D 3D7, CR-WT, -N309K, or -N330K were similarly exposed for 48 h to 0 or 2.5 mM GlcN, and proteins extracted from schizonts.Western blots were probed with anti-PfSTART1 and anti-PfEXP2 antibodies.E The densitometry of 3 biological replicates was measured (PfSTART1 signal was normalised to the corresponding PfEXP2 signal, and to the 0 mM GlcN condition; mean +/− SD).F EC 50 values of M-833 on 3D7 and CRISPR-parasites in the presence of 0, 0.25, or 2.5 mM glucosamine (GlcN).n = 3 biological replicates, mean +/− SD.Ordinary one-way ANOVA with Dunnett's multiple comparison test.*p < 0.05 (p = 0.0137).EC 50     help expand the PV space, thereby reducing the capacity of the merozoite to differentiate into a ring-stage parasite.

PfSTART1 inhibitors do not prevent sporozoite invasion but block parasite transmission to mosquitoes
Since sporozoites are known to form a PV in hepatocytes after invasion 32 , we evaluated W-991 against P. berghei sporozoite invasion to determine if PfSTART1 ortholog may be required for this process.This revealed that W-991 had no impact against P. berghei sporozoite invasion (Fig. S7A, p > 0.05).
Next, we assessed the activity of the M-833 series against the sexual stage of the P. falciparum lifecycle, in a P. falciparum dual gamete formation assay (DGFA) 33 .Mature gametocytes were treated for 48 h with 1 µM M-833 or W-991 and then gametogenesis triggered.Exflagellation and surface Pfs25 expression were quantified, which demonstrated that no inhibition of either male or female gamete formation in the presence of M-833 and W-991 had occurred (Fig. S7B).
To further evaluate this series in the sexual stage we next performed a standard membrane feeding assay (SMFA) where 3 increasing concentrations of W-991 (1, 50, 100 nM) were exposed to stage IV-V gametocytes from days 14-17.On day 17 the percentage of stage V gametocytes were evaluated which showed there was no impact of W-991 affecting gametocyte development (Fig. 7C).Media containing the compounds was then removed and treated gametocytes were fed to mosquitoes and the number of oocysts present in the midgut of mosquitoes was quantified 7 days later.This showed that W-991 exhibited transmission blocking activity of P. falciparum to mosquitoes, with 100 nM of W-991 reducing the number of oocysts by >20fold compared to untreated control (Fig. 7D, p > 0.001).To corroborate this result, we repeated the SMFA using another insectary facility which also demonstrated a reduction in transmission upon the same doses of W-991 (Fig. S7C-D), indicating that the PfSTART1 inhibitors cause irreversible inhibition of an essential process between induction of gametocytogenesis and oocyst formation, thereby showing potential as a transmission blocking antimalarial.

PfSTART1 expression, processing, and localisation in schizonts and merozoites
To better understand the biological role of PfSTART1, SLI-WT parasites were tightly synchronised and western blots were performed on various parasites stages (Fig. 8A, Fig. S8A).Following normalisation to the PfHSP70-1 loading control, PfSTART1-HA was most strongly expressed in schizonts and in very young rings as observed previously 16 (Fig. 8B).In addition to the main ~60 kDa band (corresponding to PEXEL-cleaved PfSTART1-HA), a minor processed band (around 50 kDa) was also observed (Fig. 8A).The site of cleavage is not known, but must occur near the N-terminal, since this processed band was also detected with the HA-antibody.
To further understand the processing of PfSTART1, proteins of tightly synchronised wildtype 3D7 schizonts without the HA tag were analysed every 4 h (E64 was used for the latest timepoint to prevent parasite egress; Fig. 8C).This time course demonstrates that while the majority of PfSTART1 remains as a ~48 kDa band (PEXELcleaved), two further processing events occur as schizogony progresses (PfSTART proc1 ~39 kDa, PfSTART proc2 ~36 kDa).The processing of PfSTART1-HA occurred at the N-terminus, as both processed bands could be detected by an HA-antibody on SLI-WT schizonts (Fig. S8D).
To more precisely pinpoint when PfSTART1-HA was processed during schizogony, we treated schizonts with inhibitors stalling egress at different time points (Fig. S8E-F): 49c is a plasmepsin X (PMX) inhibitor, which prevents activation of SUB1, early in the egress pathway 34 ; compound 1 (C1) is a protein kinase G inhibitor, which prevents exoneme discharge 35 ; E64 is a cysteine protease inhibitor which prevents the rupture of the red blood cell membrane, near the very end of egress 36 .The densitometry of processed bands was normalised to the PEXEL-cleaved PfSTART1, and the effect of treatment was analysed (Fig. S8F).49c-treated schizonts contained the least amount of PfSTART1 proc1 (compared to DMSO and other treatments), and C1treated schizonts had the lowest levels of PfSTART1 proc2 .E64-treated schizonts contained most of both processed bands, with a significant enrichment in PfSTART1 proc2 compared to the DMSO control.
PfSTART1 contains an unusual PEXEL motif and was recently described as not being exported to the RBC in trophozoites 37 .To investigate PfSTART1 localisation in schizonts, 3D7 schizonts were Percoll-purified and sequentially lysed in equinatoxin II (EqtII), saponin (Sap) and Triton X100 (TX100) to collect the supernatant (SN) corresponding to the RBC cytosol soluble fraction, the parasitophorous vacuole (PV) soluble fraction, and the parasite fraction, respectively (Fig. 8D and Fig. S8G).PfGBP130, a known exported soluble protein 38,39 , was detected in the EqtII SN; PfSERA5, a soluble protein that localises in the PV of schizonts 38,40 , was enriched in the Sap SN; parasite cytosolic protein PfHSP70-1 was found mainly in the TX-100 SN.PfSTART1 was most strongly detected in the TX100 fraction, indicating that it was either within the parasite, and/or associated with membranes.We tested whether PfSTART1 was fully soluble (in which case, PfSTART1 should be localised within the parasite), membraneassociated or membrane-bound.To do so, saponin-lysed 3D7 schizonts were sequentially lysed: first in PBS (with freeze-thaw cycles to break the cells), the supernatant of which was described as PBS soluble.The pellet was then incubated in sodium carbonate: the supernatant was described as carbonate soluble (proteins associated with membranes) and the pellet as carbonate insoluble (integral membrane proteins) (Fig. 8E and Fig. S8H).Controls included PfHSP70-1, PfHSP101, and PfEXP2, which were detected in the PBS soluble, the carbonate soluble, and the carbonate insoluble fractions, respectively.PfSTART1 was mainly detected in the carbonate soluble and insoluble fractions in accordance with previous findings 16 .
We then performed a proteinase K protection assay to indicate where PfSTART1 was localised after lysing different membranes as per Fig. 8E, −/+ proteinase K (Fig. 8F, Fig. S8I).PfGBP130 was localised in the RBC as it was detected in the EqtII supernatant.Cytoplasmic PfActin-1 was mostly protected from proteinase K degradation unless all the membranes were lysed in TX-100.PfPTEX150 is a PV protein 41 , and as such we expected it to be degraded mainly upon the addition of proteinase K and saponin.In our assay however, PfPTEX150 was slightly degraded by proteinase K in PBS, further degraded in saponin and completely degraded in TX-100.This suggests that the PVM in our schizonts might have been partially compromised in the PBS treatment.The incomplete degradation of PfPTEX150 in the saponin condition suggests either that the saponin lysis was not complete and some PVM remained unruptured, or that a fraction of PfPTEX150 remains inside the parasite (newly synthesised, not yet secreted into the PV).A pattern identical to PfPTEX150 was observed for PEXELcleaved PfSTART1, suggesting that more of this form of the protein is in the PV than in the parasite.PfSTART1 proc1 appears to reside within the parasite as it seems to be protected from proteinase K cleavage unless TX-100 was added.For PfSTART1 proc2 , a similar conclusion is harder to draw considering the degradation products co-migrate in the same size.Altogether this data corroborates previous work 12,16,17 that indicates PfSTART1 is most strongly expressed in schizonts, associated with membranes, and that more is located in the PV than in the parasite.

Discussion
In this study, we explored the activity of the anti-Plasmodium M-833 compound series where whole genome sequencing of resistant parasites revealed point mutations in PfSTART1 (N309K, N330K, I224F).Through structure-activity relationship studies, we produced analogues that remained on-target for PfSTART1, whilst significantly increasing the potency to achieve an EC 50 against wildtype parasites in the low nanomolar range.Engineering mutations N309K and N330K into drug sensitive parasites conferred resistance to M-833 and to the potent analogue W-991.Furthermore, knocking down PfSTART1 sensitised parasites to both M-833 and W-991.We demonstrated the direct binding of M-833 and analogues to PfSTART1, using both recombinant and native proteins.Overall, this demonstrates that M-833 and analogues exert their antiparasitic activity by targeting PfSTART1.The data presented here strongly suggest that the compounds studied inhibit PfSTART1's lipid transfer activity, but it will be important in the future to confirm this using specific transferase activity assays.
Mammalian START proteins are known to have pathological roles in several human diseases (e.g., STARD3 overexpression in cancer 42 ), including infectious diseases (e.g., human STARD11 is hijacked by intracellular Chlamydia trachomatis bacteria 43 ).Due to this, several inhibitors of human START proteins have been identified: STARD1 inhibitors C1-C6 44 ; STARD11 inhibitors HPA-12 45 and SC1 plus analogues 46 ; STARD3 (predicted) inhibitor D(-)-tartaric acid 47 and STARD3 inhibitor VS1 (low potency) 48 .The M-833 series does not show structural similarity to the aforementioned human START inhibitors and considering that the M-833 series had CC 50 > 29 µM against HepG2 cells (a cell line known to express START proteins 49 ), this indicates that our compounds are unlikely to target human START proteins.Additionally, Solvent PISA profiling suggests target specificity of M-833 series for PfSTART1 over other P. falciparum START proteins (PF3D7_1351000 and PF3D7_1463500) detected in the assay.Of note, the other proteins stabilised in the Solvent PISA assay (PfMAAP, PfRESA3 and PfSRP19) did not contain any mutations in the initial resistance selection suggesting protein binding to W-991 occurs without contributing to the mode of action of the compound.
Solvent PISA profiling described here represents a novel unbiased strategy for antimalarial drug-target identification.In addition to representing a means of orthogonal protein-engagement validation for target-candidates derived from parallel approaches, such as thermal proteome profiling, it enables the detection of drug interactions with proteins not susceptible to temperature-induced aggregation.PfSTART1 represents one of such highly thermostable protein 50 , and its interaction with the drug in parasite lysate cannot be identified based on stability shift <75 °C.Further, the Solvent PISA strategy introduced here involves major improvements over mass spectrometry-based target identification workflows established for Plasmodium [50][51][52][53] .Incorporation of Proteome Integral Solubility Alteration (PISA) assay format 54 and Data Independent Acquisition mass spectrometry (DIA-MS) 55 into the solvent assays significantly reduces the required MSanalysis time and profiling cost, as well reducing sampling bias by profiling the entire proteome across the solvent gradient.
It was initially encouraging to observe that a short 4 h treatment stopped parasite development for three days post compound removal.However, a longer follow-up of M-833 and W-991 treated (and removed) parasites indicated that they could recover over a longer period, which was confirmed by the parasite reduction ratio assay.Due to the essentiality of PfSTART1 in the blood stage 17 , it was somewhat unexpected that parasites could recover after being subjected to 10 x EC 50 of W-991 for up to five days.One possible explanation of this is that the EC 50 value derived from a standard 72 h growth assay could underestimate the concentration required to effectively kill parasites due it being unable to differentiate between stalled merozoite conversion into rings versus dead parasites.It is therefore plausible that conducting a longer growth assay (>72 h) would assist in determining a dose that would cause irreversible death.Alternatively, it is also possible that inhibiting PfSTART1 does not lead to death but rather the parasites entering a dormant mode in which they can survive until compound levels have decreased.This is reminiscent of artemisininresistant parasites whereby prolonged ring-stage has been associated with treatment survival 56,57 .Interestingly, PfSTART1 has been recently found to be significantly upregulated in both lab-adapted and fieldderived artemisinin-resistant strains that contain mutations in Kelch13 (Dd2 C580Y and Cam3.II R539T ) 58 .Additionally, artemisinin resistance has also recently been shown to be present in male gametocyte activation whereby parasites resistant to artemisinin are activated under artemisinin treatment, whilst sensitive parasites remain inactivated 59 .It would, therefore, be interesting to conduct combination experiments with our PfSTART1 inhibitors against artemisinin-resistant strains in both the asexual blood stage and mosquito stages to determine if inhibiting PfSTART1 could sensitise resistant parasites to artemisinin.
The activity profile of the PfSTART1 inhibitors was found to be variable in other stages of the lifecycle outside the blood stage with no drug effect against liver stage invasion, stage V gametocyte development, and gametogenesis.The lack of activity against the liver stage of infection was surprising given that sporozoites grow and replicate in a PV, similar to the asexual blood stages 60,61 .The apparent inactivity of the PfSTART1 inhibitors could be due to the experimental design where P. berghei sporozoites and W-991 were added to human liver cells and evaluated only 2 h post invasion.It is possible we did not capture the inhibition of PV formation and further experiments where sporozoites are added to liver cells with the compounds for a longer period may indicate growth inhibition.
We found that PfSTART1 inhibitors were active in a standard membrane-feeding assay.Whilst the functionality of PfSTART1 has been previously explored in the asexual blood stage 16,17,37 , there is little known about PfSTART1 in the mosquito stage of infection.Transcriptomic studies have shown that PfSTART1 is expressed in salivary gland sporozoites 62,63 and ookinetes 64 .Since there is no PV formation in ookinetes 60,65,66 , it is possible that PfSTART1 is utilised for another lipid-dependent process during this stage.Sporozoites, however, are known to form a PV during the invagination of the epithelial cell membrane in the mosquito salivary glands [67][68][69] which could suggest that the PfSTART1 inhibitors could act through a similar mode of action to that we see in the asexual blood stage but further investigations are required to confirm this.
To study the function of PfSTART1 in the blood stage, we tagged the C-terminal region of the protein with a 3 x HA tag using the selection-linked integration (SLI) system.Unexpectedly, we saw an increased sensitivity of the SLI-WT parasites which did not occur in the CRISPR WT parasites that did not include a tag.This may be due to the addition of the 3 x HA and P2A peptide (additional 55 amino acids) to PfSTART1, which could be interfering with its normal functioning and thereby sensitising the SLI-WT parasites to M-833.The C-terminus of PfSTART1 has been shown to be important for the regulation of lipid transfer 17 , with the last 30 amino acids of PfSTART1 predicted to form an alpha helix (Alphafold) 70,71 .
Consistent with previous reports, we found that PfSTART1 is predominantly expressed in schizonts in the asexual blood stage, is membrane-associated and is not exported into the surrounding RBC (despite its cleaved PEXEL motif) 16,37 .The close association with membranes is probably mediated by one or more interacting partner(s), considering that PfSTART1 does not contain a transmembrane domain.PfSTART1 localises partly to the PV and partly within the parasites in schizonts which suggests that PfSTART1 may have several functions, transferring lipids in several locations.
PfSTART1 appears to be processed multiple times at its N-terminus.Fréville and colleagues demonstrated that the PEXEL motif of PfSTART1 is cleaved by plasmepsin V, but that PfSTART1 is not exported 37 .Therefore, the main PfSTART1 signal observed on western blots at ~48 kDa likely corresponds to the PEXEL-cleaved protein.In addition, we demonstrated that two further processing events occur as schizogony progresses.First, the processing to PfSTART1 proc1 (~39 kDa) seems to occur early in schizogony, shortly after SUB1 activation by plasmepsin X, because plasmepsin X inhibition reduced the levels of PfSTART1 proc1 .Interestingly, PfSTART1 contains a plasmepsin X cleavage site (SDIQ 72 ), although the resulting theoretical products should be 20 kDa and 26 kDa whilst bands at 39 kDa and 36 kDa were observed.No SUB1 or plasmepsin IX cleavage sites were identified in PfSTART1 53,73 .The second processing event to PfSTART proc2 (~36 kDa) appears to occur after protein kinase G activation and accumulates considerably in the presence of cysteine inhibitor E64.Overall, it is possible that these processed forms of PfSTART1 are important in merozoites for the upcoming invasion and establishment of the PVM.PfSTART1 proc1 (and possibly PfSTART1 proc2 ) show some resistance to proteinase K after saponin lysis, indicating they are not located within the PV like the PEXEL-cleaved protein.However, both PfSTART1 proc1 and PfSTART1 proc2 only represent a small proportion of the total PfSTART1 (less than 40% of the PEXEL-cleaved form).Therefore, the functional relevance of these processed PfSTART1 remains to be investigated.
Inhibition of PfSTART1 in the blood stage led to the abnormal development of the membranes surrounding the merozoite directly after invasion as visualised with lattice light sheet microscopy.Whilst this phenotype was distinct, it remains unknown as to the composition of these lipids, in addition to the direction and location of transport.In Plasmodium, the molecular mechanisms underpinning PVM formation are still unknown 74,75 but it has recently been shown that the PVM is comprised of mostly host RBC lipids, rather than parasite-derived material 31 .Overall, our data supports a model that has been previously proposed 17 whereby PfSTART1 aids in expanding the PVM using lipids from the parasite plasma membrane (Fig. 9).How PfSTART1 is delivered to the PVM upon invasion remains to be uncovered.A simple explanation would be that it resides in secretory organelles like dense granules that are released into the developing PV space upon invasion, similar to other PV-resident proteins.Further studies utilising expansion microscopy and lattice light sheet imaging of fluorescently tagged PfSTART1 would help to resolve the localisation of PfSTART1 during and directly following invasion.Coupling these experiments with the now-confirmed PfSTART1 inhibitors would shed further light on the mechanism of action of this antimalarial series and assist in probing the function of this lipid transferase in Plasmodium.Antimalarials with new modes of action have never been more urgently needed as marked resistance to artemether-lumefantrine, the most widely used artemisinin combination therapy in Africa, has recently been found in Ugandan clinical isolates 76,77 .

Generating resistance
A clonal population of 3D7 parasites (10 8 parasites in five replicates, A to E) were exposed to 10 x EC 50 = 3 μM of MMV006833 (M-833), until most parasites died.The drug was then removed, and parasites were allowed to recover.Another cycle of drug treatment (3 μM M-833) was then resumed.The resistant lines were cloned out by limiting dilution (diluting the culture into a 96-well plate to achieve an average of ~0.3 parasites per well), prior to growth inhibition assays and genomic DNA (gDNA) extraction (DNeasy Blood and Tissue kit (Qiagen) 18 .
Molecular biology and transfection of P. falciparum: Selection Linked Integration and CRISPR/Cas9 methods Methods for making and transfecting plasmids into P. falciparum parasites are described in the Supplementary Information and DNA primer sequences are listed in Table S2.

Chemistry procedures
Methods for making the M-833 analogues used in this work are outlined in the Supplementary Information.

Whole genome sequencing and genome reconstruction
Sequencing of M833-resistant parasites and parental 3D7 line was performed as previously described 18 .Genomic sequencing data is available from the European Nucleotide Archive; accession number PRJEB65444.

Growth inhibition assays
Parasite growth assays with inhibitory compounds were performed as per 11 and are fully described in the Supplementary Information.

Egress, invasion & recovery assay
To measure egress, invasion, and follow-up recovery in the presence of different compounds, we adapted the method developed in 11 , using Hyp1-Nluc parasites 29 .A full description is in the Supplementary Information.

Stage arrest and recovery assay
Parasites were synchronised using a Percoll density gradient (Cytiva) combined with 5% sorbitol lysis.Ring-stage parasites at 0-4 hours postinvasion at 2% parasitemia and haematocrit were then added to 2 µM M-833, 60 nM W-991 or 0.02% DMSO and incubated at 37 °C with compound replenished every 24 h.After 48 h, samples of M-833 and W-991-treated parasites were washed x 3 in complete RPMI to remove the compound and put back into culture for the remainder of the experiment.For the following two cycles of growth, samples were taken every 24 h to monitor recovery.Upon completion of time points, fixed cells were stained with 2.5 x SYBR Green (Invitrogen) in PBS, washed once in PBS, and analysed on the Attune Flow Cytometer (ThermoFisher Scientific).Giemsa-stained blood smears were visualised and imaged using a Nikon Eclipse E600 microscope.

Parasite reduction ratio assay
This was performed as per 78,79 and the full protocol is in the Supplementary Information.

Lattice light sheet imaging
Parasites were sorbitol synchronised at days 5 and 2 prior to filming.To prepare the parasites for filming, culture was loaded on LS columns attached to MACS MultiStand (Miltenyi Biotec) to isolate late-stage parasites.Imaging medium was prepared by adding 10 μM Trolox (Santa Cruz 53188-07-1) to the culture medium.To compare the effect of drug treatment on parasite invasion, either 60 nM of W-991 or an equivalent DMSO concentration was added to the imaging medium.RBCs were resuspended at 0.5% hematocrit in RPMI-HEPES supplemented with 0.2% sodium bicarbonate and stained with 1.5 μM Di-4-ANEPPDHQ (Invitrogen D36802) membrane marker for 1 h at 37 °C.The stained RBCs were then washed 3 x and resuspended in imaging medium.Purified schizonts were resuspended in culture medium and incubated with 25 nM Mitotracker Deep Red FM (Invitrogen M22426) for 30 min at 37 °C, 5% CO 2 .The stained schizonts were then pelleted and the supernatant removed before resuspending the schizonts in the imaging medium.Before imaging, imaging medium with drug was loaded to a well and imaging medium with DMSO control was loaded to another well on an 8-well glass bottom plate (Ibidi 80807).Stained RBCs and stained schizonts were then added to each well and let settle for 30 min.The imaging experiments were performed on Zeiss Lattice Lightsheet 7. 488 nm laser was used to excite Di-4-ANEPPDHQ and 640 nm laser was used to excite Mitotracker Deep Red FM.A quadnotch filter was used to block 405/488/561/640 nm excitation lights.A 290 μm x 200 μm region was scanned with 0.3-0.4μm interval for both drug-treated and control wells and a simultaneous timelapse was acquired at 2-3 ms exposure time with 15 s interval for 2 h.Acquired data were deskewed and deconvolved using Lattice Lightsheet processing on ZEISS ZEN Blue 3.4 software, then cropped into smaller regions for analyses.

Vacuole tracking
A data subset was used as training dataset for machine learning on Aivia 10.5.1 software.Single 3D frames were chosen and annotated as either 'Background' or 'Want' on Pixel Classifier analysis tool, where 'Want' is the vacuole area.The Pixel Classifier was applied on other data as feedback for training.Once the trained Pixel Classifier reached satisfactory accuracy, it was applied on the whole dataset in batch mode to obtain 'Want' channel, which is the vacuole confidence map, as additional channel to each file.3D Object Tracking was then performed based on the 'Want' channel and the vacuoles of interest were isolated from the tracked objects.The sphericity of the vacuoles was extracted from the statistics of the isolated objects.The sphericity data were then plotted on GraphPad Prism 9.5.0 and two-tailed nested t-test was performed on the software to obtain p-value between the vacuole sphericity of drug-treated and DMSO control conditions at the initial condition and 15 min after the vacuole is formed.

Recombinant Expression and Purification
Codon optimised (Spodoptera frugiperda, Sf) START domain alone (I149-V394 from PF3D7_0104200) was synthesised by Integrated DNA Technologies and cloned into a modified baculovirus transfer vector (pAcGP67-A) with a N-terminal GP67-signal sequence, 8xHis tag, and TEV protease cleavage site.Recombinant baculovirus was generated via the flashBACTM Baculovirus Expression System using Sf21 cells (Life Technologies), amplified to reach a third passage viral stock, and 1.5% used to infect Sf21 for protein expression.After incubation for three days at 28 °C, media containing secreted protein was harvested by centrifugation and supplemented with 50 mM Tris pH 7.5, 20 mM MgCl 2 , 100 mM NaCl, and 10-20 mM imidazole.The supplemented protein sample was purified by nickel affinity chromatography (HisTrap Excel 5 mL, Cytiva), and eluted with 20 mM Tris pH 7.5, 500 mM NaCl, 300-500 mM imidazole.Eluted fractions were further purified using size-exclusion chromatography (Superdex S75 16/600 or Superdex 10/300, Cytiva) with the column pre-equilibrated with 20 mM HEPES pH 7.5, 150 mM NaCl, and peak-containing fractions concentrated and stored at -80 °C until required.If required, prior to ITC, a second size-exclusion chromatography step was performed to remove soluble aggregates.Technologies, and then I149 forward and V394 reverse primers utilised to clone the same I149-V394 construct boundaries into the modified pAcGP67a expression vector with a N-terminal GP67-signal sequence, 8xHis tag, and TEV protease cleavage site.As a result of the subcloning, SSG was removed upstream of the TEV cleavage site.Construct boundaries and incorporation of the N309K and N330K mutations was verified by Sanger sequencing.The PfSTART1 mutants were expressed and purified as per wild-type PfSTART1.

PfSTART1 polyclonal antibody generation and purification
Polyclonal rabbit anti-PfSTART1 antibodies were generated by the WEHI Antibody Facility using recombinant PfSTART1 (I149-V394).We further purified the polyclonal antibodies using a PfSTART1 affinity column that was generated using the AminoLink® Plus Immobilisation Kit (Thermo Scientific), according to the manufacturer's instructions.
For this, we immobilised deglycosylated (PNGaseF, NEB) and TEV cleaved PfSTART1 domain protein that had been further purified by nickel affinity chromatography to remove N-linked glycans and the 8xHis tag.The polyclonal antibody was purified on this matrix according to the manufacturer's instructions.

Isothermal titration calorimetry
ITC experiments were performed on a MicroCal PEAQ-ITC calorimeter at 25 °C, with a stirring speed of 750 rpm and a reference power of 5 μcal/sec.500 μM M-833 series inhibitor solutions were diluted to 10 μM in 50 mM NaPO 4 -3 (pH 7.4), 150 mM NaCl with a final DMSO concentration of 2% (v/v) (cell sample).PfSTART1 protein was dialysed extensively against 50 mM NaPO 4 -3 (pH 7.4), 150 mM NaCl, before diluting to 90 μM in the same buffer with 2% (v/v) DMSO (syringe sample).The first injection was 0.4 μL over a 0.8 s duration, and the remaining 19 injections were 2 μL of 4 s duration, with 150 s injection spacing.Data were collected and analysed using the PEAQ-ITC software (MicroCal) and fit by a single site binding model.A fitted offset (constant control heat) was also applied to the integrated heat.

Solvent profiling Western Blot
3D7 parasites at schizont stage were harvested with 10 x pellet volume of 0.15% saponin in PBS.To extract soluble material, 10 x pellet volume of 0.4% NP40 (IGEPAL CA-630, Sigma-Aldrich) in PBS with 1x Complete Protease cocktail tablet (Sigma-Aldrich) was added to the parasite pellets and 3 cycles of freeze/thawing was performed.Samples were then mechanically sheared by passage through 25 G and 30 G needles before lysate was cleared via centrifugation at 16,000 g x 30 min at 4 °C.Soluble fractions were collected and stored at −80 °C until use.

MS sample preparation
Sample preparation for proteomic analysis was carried out using modified SP4 glass beads protocol 80 .In brief, protein was reduced (20 mM TCEP, 100 mM TEAB) for 20 min at 55 °C and alkylated with 55 mM 2-Chloroacetamide for 30 min, followed by precipitation on beads in 80% ACN with a 6 min centrifugation at 21,000 g and 3 x wash with 80% Ethanol.Dried beads were subjected to sequential digestion with LysC (3 h, 1:50 ratio w/w) and trypsin (overnight, 1:50 ratio w/w), and the resulting digest was acidified with 1% TFA and desalted on T3 C18 stage tips (Affinisep) according to manufacturer's specifications.

MS data acquisition and data analysis
Peptide samples were analysed on Orbitrap Eclipse Tribrid mass spectrometer that is interfaced with the Neo Vanquish liquid chromatography system.Samples were loaded onto a C18 fused silica column (inner diameter 75 µm, OD 360 µm × 15 cm length, 1.6 µm C18 beads) packed into an emitter tip (IonOpticks) using pressurecontrolled loading with a maximum pressure of 1,500 bar, that is interfaced to the mass spectrometer using Easy nLC source and electro sprayed directly into the mass spectrometer.Sample separation was carried out on a linear gradient 5% to 17% of solvent-B at 400 nL /min flow rate (solvent-B: 80% (by vol) acetonitrile) for 23 min and 17% to 25% solvent-B for 10 min, 25% to 34% for 11 min and 34% to 90% solvent-B for 1 min which was maintained at 90% B for 3 min and washed the column at 2% solvent-B for another 3 min comprising a total of 63 min run with a 45 min gradient in a data independent acquisition (DIA) mode MS scan parameters included a full scan using an orbitrap and 120,000 resolution, standard AGC target and maximum injection time of 30 ms.A second DIA experiment used the orbitrap at 30,00 resolution, a precursor scan range of 200-1200 m/z with a normalized AGC target of 3000% and 45 variable isolation windows.Peptides were isolated and fragmented using stepped collision-induced dissociation (HCD) at 24%, 28% and 32% normalized collision energy.Peptide identification was carried out in DIA-NN 1.8.1 using standard settings and an in silico spectral library generated from Uniprot P. falciparum (UP000001450) and human (UP000005640) reference proteomes.The peptide length range was set to 7-30 amino acids.One missed cleavage and 1 variable modification were allowed (ox(M) and Ac(Nterm)).Precursor FDR was set to 1% and the match between runs was on, precursor charge range was set between 1-4, precursor m/z range of 300-1800, fragment ion m/z range of 200-1800.Subsequently, the differential abundance analysis (moderated t-test, based on limma package 81 ) of P. falciparum proteins was conducted in the R environment (R version 4.2.0;R Studio Version 2023.12.1 + 402) using precursor normalised MaxLFQ data for proteins detected with ≥2 peptides.Hit selection criteria included p value < 0.01, log2 fold change of >0.73 in protein abundance and protein detection across all tested samples.

Gametocyte culturing and standard membrane feeding assays
These assays were performed at the Walter and Eliza Hall Institute and at the London School of Hygiene and Tropical Medicine using slightly different approaches which are described in the Supplementary Information.
Generation of Plasmodium berghei sporozoites P. berghei ANKA constitutively expressing mCherry 82 was used for the in vitro liver stage invasion assay.Animals used for the generation of the sporozoites were 4-to 5-week-old male Swiss Webster mice and were purchased from the Monash Animal Services (Melbourne, Victoria, Australia) and housed at 22 to 25 °C on a 12 h light/dark cycle with 40-70% humidity at the School of Biosciences, The University of Melbourne, Australia.All animal experiments were in accordance with the Prevention of Cruelty to Animals Act 1986, the Prevention of Cruelty to Animals Regulations 2008, and National Health and Medical Research Council (2013) Australian code for the care and use of animals for scientific purposes.These experiments were reviewed and permitted by the Melbourne University Animal Ethics Committee (2015123).
Infections of naïve Swiss mice were carried out by intraperitoneal (IP) inoculation obtained from a donor mouse between the first and fourth passages from cryopreserved stock.Parasitemia was monitored by Giemsa smear and exflagellation quantified 3 days post-infection.A. stephensi mosquitoes were allowed to feed on anaesthetised mice once the exflagellation rate was assessed between about 12 to 15 exflagellation events per 1 × 10 4 RBCs.Salivary glands of infected mosquitoes (days 17 to 24 post-infection) were isolated by dissection and parasites placed into RPMI-1640 media.

In vitro liver invasion assays
This was performed essentially as described 83 with the minor variations outlined in the Supplementary Information.

Dual gamete formation assays
The compounds were tested in the P. falciparum Dual Gamete Formation Assay (PfDGFA) 84 , fully described in the Supplementary Information.

Protein extraction & Western Blot
Parasites were prepared for the stage of interest and proteins were extracted using a saponin-lysis (unless indicated otherwise).For knock-down assays, parasites were exposed to 0 or 2.5 mM GlcN for 48 h (starting from late schizont/early rings): the day before harvesting schizonts, 30 nM ML10 (LifeArc) was also added to the cultures to block egress.Infected red blood cells were lysed 10 min on ice with 0.1% saponin in PBS complemented with 1x Protease Inhibitors Cocktail (Roche; PBS + PI).Parasites were pelleted and washed with PBS + PI to remove haemoglobin.The pellets were resuspended in 10 to 20 x volume of non-reducing sample buffer (NRSB; 50 mM Tris-HCl pH 6.8, 2 mM EDTA, 2% SDS, 10% glycerol, 0.005% phenol blue), sonicated a minimum of 3 × 30 seconds (Diagenode sonicator), were optionally reduced with 100 mM dithiothreitol, and boiled 10 min at 80 °C.Protein samples were centrifuged at 15,000 g, then run on a pre-cast 4-12% NuPAGE Bis-Tris gel (Invitrogen) and proteins were transferred onto a nitrocellulose membrane using iBlot (Invitrogen).Membranes were exposed to the primary antibody overnight at 4 °C, to the secondary antibody 1 h at room temperature, and fluorescence was measured using an Odyssey imaging system, which was also used to measure densitometry.The list of antibodies used, their dilution, and origins are described in Table S3.

Differential lysis assay
Schizont-infected RBCs were sequentially lysed with (1) equinatoxin II (EqtII), which lyses the RBC membrane, (2) saponin, which lyses the PVM, (3) TX-100, which lyses all membranes.All the lysis buffers were made in PBS + PI, supernatants were collected in fresh tubes, and pellets were washed twice in PBS + PI.ML10-treated schizonts were enriched (with Percoll) and lysed in 10 x pellet volume of EqtII (at a concentration empirically determined to obtain 100% hemolysis) for 10 min at 37 °C.Lysate was centrifuged at 1000 g for 5 min to collect the supernatant.The pellet was washed and then lysed in 10 x volume of 0.03% saponin (10 min on ice, centrifuged at 16,000 g for 1 min at 4 °C).The supernatant was collected, the pellet was washed and lysed in 10 x volume of 0.25% TX-100 (10 min on ice, centrifuged at 16,000 g for 1 min at 4 °C, and the supernatant collected).All supernatants were mixed with NRSB, boiled 10 min at 80 °C, and separated by electrophoresis as explained previously.

Carbonate extraction and Proteinase K protection assay
These were conducted as previously described 38,85 with full details in the Supplementary Information.

Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.

Fig. 1 |
Fig. 1 | M-833 resistant parasites contain mutations in the P. falciparum START1 protein.A Structure of MMV006833 (M-833).B Parasites populations (Pop) resistant to M-833 were generated by exposing 3D7 P. falciparum parasites to 10 x EC 50 of M-833 three times.These parasites were cloned, and growth inhibition assays performed in the presence of 10 µM to 0.04 µM M-833.Growth inhibition was normalised to DMSO controls.Average of EC 50 values and 95% confidence intervals are indicated; n = 3 biological replicates.Source data are provided as a Source Data file.C Key domains and cartoon representation of the AlphaFold predicted structure of the StAR-related lipid transfer protein, PfSTART1 (PF3D7_0104200) 70,71 .Mutations that had been identified in the M-833 resistant parasite populations are shown as sticks (yellow = N309K, N330K; green = I224).

Fig. 2 |
Fig. 2 | Engineering mutations N309K and N330K in PfSTART1 confers resistance to M-833.Wild-type (WT) or mutant (N309K, N330K) pfstart1 genes were introduced in drug-sensitive 3D7 parasites using a Selection-Linked Integration (SLI) method or using CRISPR-Cas9 (CR).A Schematic of the SLI cloning strategy.The 5' arm of native pfstart1 was amplified, PCR sewn to a recodonised version of the 3' arm (either wildtype (WT), N309K or N330K), inserted into a p-HA-2A-Neo-glmS plasmid and transfected into 3D7 parasites, to be integrated into the native gene.B Growth inhibition curves of M-833 treated 3D7 and SLI-parasites.Parasites were incubated in various concentrations of M-833 and growth was assessed 72 h later using a lactate dehydrogenase activity assay.EC 50 values and 95% confidence intervals are indicated below the graph; n = 3 biological replicates, mean +/− SD.C Schematic of the CRISPR cloning strategy.The full length 5' homology blocks for WT, N309K, and N330K were amplified from the SLI plasmids and inserted into the p1.2-glmS 86 plasmid, along with the native 3' arm of pfstart1.Note that this construct does not include a HA tag.The plasmids were transfected into 3D7 parasites with Cas9 enzyme complexed with a guide RNA, to be integrated into the native gene.(D) Growth inhibition curves of M-833 on 3D7 and CRISPR-parasites (same as B).n = 3 biological replicates, mean +/− SD.Source data are provided as a Source Data file.
images of nascent PVMs indicated the mean sphericity of the PVM of W-991-treated merozoites stayed high during the observation period compared to the DMSO control where the PVM became less spherical consistent with change in shape to accommodate the amoeboid ring (Fig.7B, Fig.S6A, p = 0.045 at 15 min).The mean vacuole surface area and volume of the PVM were also reduced in W-991 but this did not reach significance (Fig.S6B, C; p = 0.109, p = 0.129, respectively).Overall quantification of the lattice light sheet microscopy supports our earlier empirical observations that inhibition of PfSTART1 prevents normal development of the parasite plasma membrane and/or PVM to A.

Fig. 5 |Fig. 6 |
Fig. 5 | The M-833 series demonstrates target engagement for PfSTART1 while the mutant form prevents compound binding.A Isothermal titration calorimetry (ITC) analysis of M-833 series and recombinant PfSTART(WT) or PfSTART(N330K).Representative thermograms of 90 µM PfSTART1 titrated into 10 µM M-833, W-991, and negative control 4. The bottom panel comprises the data after integration of the peaks and a fitted offset applied.The binding curve shows the fit to a single-site binding model.DP = differential power.The table represents summary of the mean affinity, enthalpy, and entropy obtained for M-833, W-991 and 4 binding to PfSTART1 from n = 2 experiments.Error represents standard deviation.ND = not determined.The second replicate and values for individual parameters are provided in Fig. S4C.B Solvent proteome profiling assays.Parasite lysate was treated with DMSO or 10 µM W-991, challenged with an acetic acid/ethanol/formic acid mixture (AEF) and soluble fractions extracted and analysed via western blots probed with anti-PfSTART1.Western blot replicates are shown in Fig. S4D.C Volcano plots depict differential soluble protein abundance analysis (moderated t-test based on limma package) of parasite lysate treated with W-991 (100 µM) or the DMSO control (n = 3 biological replicates, mean +/− SD) after solvent-induced protein precipitation (7-25% AEF).Non-significant (ns) proteins are plotted in grey, and significant stabilised proteins are in red.Hit selection cut-offs of 0.73 log2 fold-change and p < 0.01 are indicated with dashed lines.Significant stabilisation hits are shown in the bar graphs representing the average of three biological replicates of relative soluble protein abundance in DMSO-or W-991-treated parasite lysate samples after solvent-induced protein precipitation, plotted for Gradient 1 'G1' (7-15% AEF) and Gradient 2 'G2' (17-25% AEF).Error bars represent standard deviation.Source data are provided as a Source Data file.

Fig. 7 |
Fig.7| W-991 blocks merozoite transition to ameboid ring in RBCs, and blocks transmission in mosquitoes.A Representative images of 4D Lattice Light Sheet Microscopy of merozoites (MitoTracker; cyan) directly after invasion of RBCs (all membranes are magenta).Treatment with W-991 shows disruption to the formation of the parasitophorous vacuole membrane (magenta) compared to vehicle control.B The mean vacuole sphericity (+/− SD) for W-991 and DMSO treatments across 30 min filming period.Statistical analysis was conducted via a Nested t-test (two-tailed) in GraphPad Prism showing p = 0.716 at T = 0 mins and a p = 0.045 at T = 15 mins (*).Across three independent experiments, there were 20 and 22 events analysed for W-991 (60 nM) and DMSO (0.001%) treatments, respectively (Fig.S6and Movie S1 and S2).C Stage V gametocytes were quantified on day 17 which demonstrates there was no defect in gametocyte development upon W-991 treatment compared to PMIX/X dual inhibitor WM382.D Oocysts were quantified in mosquito midguts on day 7 after feeding which showed a dose-dependent decrease in oocysts per mosquito with W-991 treatment.Statistical tests (two-way ANOVA) were performed in GraphPad Prism.**** indicates p < 0.0001.Positive control compound, WM832, was used at 50 nM53 .Error bars indicate SD for n = 30 mosquitoes for each treatment (mean+/−SD).Data are a single representative from 3 independent experiments which can be found in FigureS7C, D. Source data are provided as a Source Data file.

Fig. 8 |
Fig.8| PfSTART1 expression and processing.A Western blot of SLI-WT parasites along the 48 h erythrocytic cycle.A saponin-lysis was performed on tightly synchronised SLI-WT young rings, older rings, young trophozoites, older trophozoites, and schizonts.B Densitometry of PfSTART1 over three biological replicates (normalised by the corresponding PfHSP70-1 signal; mean +/− SD).C Synchronous 3D7 trophozoites were magnet-purified, and whole cells harvested at ~40 hpi (hour post invasion), ~44 hpi and ~48 hpi (this last sample was treated with 10 µM E64 to prevent egress).PEXEL-cleaved PfSTART1 and two further processed forms are indicated by solid and empty arrows respectively.D To localise PfSTART1, Percollpurified 3D7 schizonts were sequentially lysed with equinatoxin II (EqtII), saponin (Sap) and Triton-X100 (TX-100), and the supernatants (SN) were collected to harvest proteins localising in the red blood cell (RBC) cytosol, the parasitophorous vacuole (PV) and the parasite, respectively.PfGBP130 is a protein known to be exported into the RBC cytosol; PfSERA5 localises to the in schizonts; PfHSP70-1 is a parasite cytosolic protein.E To determine the solubility of PfSTART1, saponinlysed 3D7 schizonts were sequentially lysed in PBS (with five freeze-thaw cycles) and sodium carbonate.The supernatant resulting from the PBS lysis was collected (PBS soluble), as well as the supernatant and the pellet of the carbonate lysis (Carbonate soluble and Carbonate insoluble respectively).Controls are the soluble protein PfHSP70-1, the membrane-associated protein PfHSP101, and the integral transmembrane protein PfEXP2.F Proteinase K protection assay was conducted on Percoll-purified 3D7 schizonts.Schizonts were first lysed in EqtII, the supernatant (SN) of which was collected.The remaining parasite and PV were either incubated in PBS (no lysis), saponin (PVM-lysis), or TX-100 (lysis of all membrane) with or without proteinase K. PfGBP130 is RBC cytosolic protein; PfPTEX150 is a PV protein; PfActin-1 is a parasite cytosolic protein.Note that another replicate for each of these experiments is shown in Fig S8.Source data are provided as a Source Data file.

Fig. 9 |
Fig. 9 | The M-833 series blocks the conversion of newly invaded merozoites into ring-stage parasites by inhibiting the PfSTART1 protein and reducing the expansion of the parasitophorous vacuole membrane (PVM).(Top) After invasion, the merozoite begins to spin and produce pseudopodia before differentiating into an amoeboid ring-stage parasite.(Bottom) After invasion PfSTART1, is delivered into the vacuole space possibly via secretory organelles fusing with the parasite plasma membrane (PPM).Here PfSTART1 could transfer phospholipids from the PPM to the PVM to allow the latter to expand to accommodate the growing ring-stage parasite.The M-833 compound series tightly binds to PfSTART1, probably displacing the phospholipids and thereby preventing the expansion of the PVM and arresting parasite growth.

B. C. 3D7 SLI- WT SLI- N309K SLI- N330K
values and 95% confidence intervals are indicated in the table.Source data are provided as a Source Data file.