The great escape: a Shigella effector unlocks the septin cage

Shigella, an important human pathogen, can secrete effector proteins to invade host cells and evade mechanisms of cell-autonomous immunity. In a new manuscript published in Nature Communications, Xian et al. report that the Shigella kinase effector OspG promotes the ubiquitination of septin cytoskeletal proteins to evade cage entrapment.

The great escape: a Shigella effector unlocks the septin cage Shigella is a causative agent of dysentery responsible for over 200,000 deaths per year.For infection of human cells, Shigella employs a Type 3 Secretion System (T3SS) to deliver ∽30 effector proteins in a highly orchestrated manner.While early effectors promote host cell invasion and escape from the phagocytic vacuole to the cytosol, late effectors mediate evasion of host immune defenses, including mechanisms of cell-autonomous immunity.Cell-autonomous immunity is the ability of immune and non-immune cells to protect themselves against intracellular pathogens using evolutionarily conserved defense pathways 1 .As a human-adapted intracellular pathogen, Shigella's ability to counteract cell-autonomous immunity has been the focus of intense investigation.However, our mechanistic understanding is limited as Shigella effectors often have pleiotropic roles and target host pathways in a multi-layered manner.Prominent examples of effectors important for Shigella virulence include (i) IcsB and VirA that mediate escape from antibacterial autophagy 2,3 , (ii) IpaH9.8 that counteracts guanylate-binding protein (GBP)-mediated immunity 4,5 , (iii) OspC3 that targets Caspase-4 and Caspase-11 to avoid inflammatory cell death 6,7 , and (iv) OspC1 and OspC3 that supress the expression of interferon-inducible immune genes 8 (Fig. 1A).
Septins are a family of cytoskeletal proteins with key roles in cell division and host-pathogen interactions 9 .As compared to other cytoskeletal proteins, septin assembly is unconventional-different family members form hetero-oligomeric complexes, which assemble into filaments and interact with actin, microtubules, and membranes.Septin biology has been historically linked to posttranslational modifications (PTMs), although the precise role of PTMs in septin assembly and function is mostly unknown.Septins were among the first proteins reported to be modified by SUMOylation (a ubiquitin-like PTM), and work has shown roles for SUMOylation in septin filament bundling and cell division 10 .A role for the cytoskeleton in cell-autonomous immunity was discovered from studying septin-Shigella interactions, where cytosolic bacteria are compartmentalized by septin cage-like structures for targeting to antibacterial autophagy 11 .Over a decade of research performed in vitro using purified proteins, in cellulo using tissue culture cells, and in vivo using zebrafish infection models has shown that septin cages are antibacterial, preventing Shigella replication and dissemination [11][12][13] .However, why only a fraction of cytosolic bacteria are recognized by septin caging is poorly understood, and whether Shigella effectors can manipulate septin biology has been a long-standing question in the field.Reporting in Nature Communications, Xian et al. discover that a Shigella kinase effector OspG targets host ubiquitination pathways to inhibit septin assembly and evade cage entrapment 14 .
A newly identified role for Shigella OspG to counteract septin-mediated cell-autonomous immunity Ubiquitination is a well-studied PTM that plays a pivotal role in host-pathogen interactions.Ubiquitination is achieved by the sequential action of three sets of enzymes: ubiquitin-activating (E1), ubiquitin-conjugating (E2), and ubiquitin ligases (E3), that together catalyze a wide range of ubiquitin signals.Despite the vast repertoire of E1, E2, and E3 enzymes present in human cells, relatively few Shigella effectors have been found to target host ubiquitination pathways.In this case, work has shown that (i) IpaH1.4 and IpaH2.5 antagonize M1 ubiquitin chains by targeting the E3 ligase LUBAC to degradation 15,16 , and (ii) OspG and OspI bind E2 enzymes (including UbcH5b and UBC13, respectively) to downregulate host immune responses mediated by nuclear factor-κB (NF-κB) [17][18][19] .
For their study 14 , Xian et al. set out to investigate the kinase activity of OspG, whose precise molecular target had remained elusive for many years.The use of large-scale phosphoproteomics identified CAND1 (cullin-associated NEDD8-dissociated protein1), a key assembly factor of the E3 family of cullin-RING ubiquitin ligases (CRLs), as being phosphorylated by OspG.CAND1 was phosphorylated on several serine and tyrosine residues, indicating that OspG acts as an unusual dual serine/tyrosine kinase.Pull-down assays and liquid chromatography-mass spectrometry (LC-MS) showed that OspGmediated phosphorylation of CAND1 regulates its interaction with several cullins, suggesting that its impact on host E3 ligase activity is widespread.Next, Tandem Ubiquitin Binding Entities (TUBE) pull down in cells was used to profile the impact of OspG on the host ubiquitome during Shigella infection and found several cellular pathways to be affected, including the NF-κB pathway.Strikingly, multiple septin family members (SEPT2, SEPT7, SEPT9, SEPT10, and SEPT11) presented higher levels of ubiquitination in cells infected by Shigella in the presence of OspG.Immunoprecipitation experiments with flagtagged septins co-expressed with OspG confirmed robust ubiquitination of SEPT6, SEPT9, and SEPT10; weaker ubiquitination was observed for SEPT2, SEPT7, and SEPT11.Taken together, these findings demonstrate that phosphorylation of CAND1 by OspG causes a global shift in substrate ubiquitination through CRLs, and in this way, the kinase activity of OspG promotes ubiquitination of multiple septin family members.Considering recent work showing that septin filaments based on hetero-octameric complexes (SEPT2-SEPT6-SEPT7-SEPT9-SEPT9-SEPT7-SEPT6-SEPT2) link actin stress fibers to membrane 20  the previously reported role for SEPT9 in Shigella cage entrapment 11 , these new data support the hypothesis that septin filaments associated with the bacterial surface are based on hetero-octameric complexes.How does ubiquitination modify septin assembly and function?It is well known that different ubiquitin signals can preferentially direct substrates towards different cellular pathways.For example, K63linked polyubiquitin chains target substrates to autophagic degradation, whereas K48-linked polyubiquitin chains target substrates to proteasome degradation.Shigella-septin caging has previously been associated with ubiquitination and antibacterial autophagy, where immunostaining experiments and super-resolution microscopy suggested that septins and K63-linked polyubiquitin chains form separate microdomains on the bacterial surface 11,21 .For their study 14 , Xian et al. show that ubiquitinated SEPT9 contains mixed K6-, K27-, and K33linked polyubiquitin chains, suggesting non-canonical roles for ubiquitination in septin biology beyond protein degradation.Considering this, the authors tested if OspG-mediated ubiquitination regulates the interaction between different septin family members.Immunoprecipitation experiments revealed a reduced association of SEPT9 with SEPT6 and SEPT7 in the presence of OspG expression, demonstrating that septin hetero-oligomeric complex formation and higher-order assembly are partially impaired upon ubiquitination.In agreement, the authors used quantitative microscopy and observed significantly more Shigella septin cages (~25%) in cells infected with an ospG-deletion mutant as compared to cells infected with OspG-expressing bacteria (~15%).It is worth noting that the specific construction of the ospG deletion mutant is important since a mxiE-deletion mutant (mxiE encodes a transcription factor responsible for upregulation of late effectors, including OspG) failed to promote septin cage entrapment.Immunostaining infected cells with FK2 (an antibody shown to recognize both mono-and polyubiquitinated proteins) showed that ubiquitin is recruited to SEPT9 and SEPT7 cages that entrap bacteria expressing OspG, supporting a role for OspG in septin ubiquitination.From these observations, the authors conclude that OspG promotes ubiquitin-mediated deconstruction of the Shigella-septin cage.

Perspectives
In summary, Xian et al. have discovered a mechanism evolved by Shigella to counteract cell-autonomous immunity by ubiquitinating septins in an OspG-dependent manner (Fig. 1B).This discovery is exciting for both infection and cytoskeleton biology, highlighting a fundamental role for non-canonical ubiquitin linkages in the prevention of septin assembly.It is next of great interest to investigate the mechanisms and structural details underlying the ability of ubiquitin to prevent hetero-oligomeric complex formation and higher-order septin assembly.More broadly, Xian et al. provide insights into the manipulation of host ubiquitination pathways by Shigella and expand the breadth of cellular processes regulated by ubiquitination.Considering this newly identified kinase-dependent role for OspG, the phosphorylation of CAND1 to manipulate CRL complex activity may emerge as a common regulatory mechanism in eukaryotic cells.In the future, interdisciplinary approaches involving bacterial infection (using Shigella and other intracellular pathogens), 'omics technologies, and high-throughput/high-content screening are likely to reveal further roles for PTMs in septin biology and cellular homeostasis 22 .
Beyond evasion of septin cage entrapment, what are the downstream consequences of OspG-mediated ubiquitination on host defense?Considering that OspG globally modifies the host ubiquitome, it is important to determine the multiple roles of OspG in the regulation of cellular and immune processes in addition to septin ubiquitination.Although Xian et al. do not perform survival assays or in vivo work to test for virulence of bacteria lacking ospG, it is well known that investigation of septin biology at the cellular and whole animal level is highly complex due to their diverse roles in cellular homeostasis.To address this, single-cell analysis focusing on Shigella septin cages undergoing OspG-mediated deconstruction may be required to fully characterize the impact of ubiquitination on entrapped bacteria.Studying how septin assembly and ubiquitination are coordinated with each other, and also with other cellular processes (such as antibacterial autophagy and GBP recruitment), will be necessary for a complete understanding of cell-autonomous immunity.As we better understand these cellular processes, we can aim to develop therapies that target them for enhanced infection control.

GBPs
Ana T. López-Jiménez, Gizem Özbaykal Güler & Serge Mostowy Check for updates Shigella, an important human pathogen, can secrete effector proteins to invade host cells and evade mechanisms of cell-autonomous immunity.In a new manuscript published in Nature Communications, Xian et al. report that the Shigella kinase effector OspG promotes the ubiquitination of septin cytoskeletal proteins to evade cage entrapment.