Q586B2 is a crucial virulence factor during the early stages of Trypanosoma brucei infection that is conserved amongst trypanosomatids

Human African trypanosomiasis or sleeping sickness, caused by the protozoan parasite Trypanosoma brucei, is characterized by the manipulation of the host’s immune response to ensure parasite invasion and persistence. Uncovering key molecules that support parasite establishment is a prerequisite to interfere with this process. We identified Q586B2 as a T. brucei protein that induces IL-10 in myeloid cells, which promotes parasite infection invasiveness. Q586B2 is expressed during all T. brucei life stages and is conserved in all Trypanosomatidae. Deleting the Q586B2-encoding Tb927.6.4140 gene in T. brucei results in a decreased peak parasitemia and prolonged survival, without affecting parasite fitness in vitro, yet promoting short stumpy differentiation in vivo. Accordingly, neutralization of Q586B2 with newly generated nanobodies could hamper myeloid-derived IL-10 production and reduce parasitemia. In addition, immunization with Q586B2 delays mortality upon a challenge with various trypanosomes, including Trypanosoma cruzi. Collectively, we uncovered a conserved protein playing an important regulatory role in Trypanosomatid infection establishment.

limited response to chemotherapy, and showing unresponsiveness towards vaccination.
Nagana (Trypanosoma brucei brucei, Trypanosoma congolense, Trypanosoma vivax), Surra (Trypanosoma evansi) or Dourine (Trypanosoma equiperdum) are the major forms of AAT 2 .Since the occurrence of Nagana is an important constraint for cattle production, it has a great impact on the nutrition of millions of people living in AAT endemic areas, and on the agriculture economics, resulting in an estimated annual economic cost of about US$ 4 billion 7,8 .Murine models, which are more easily amenable compared to cattle or other domestic animals, are very useful tools to study AT.In this respect, a murine T. b. brucei infection exhibits characteristics of HAT and is often the model of choice.
Due to millions of years of co-evolution, trypanosomes have been able to thwart host innate immune responses and escape early immune recognition, allowing the initiation of infection and a sufficiently long survival in their mammalian host to complete their life cycle and transmission 9,10 .However, the molecular machinery that Trypanosoma species employ to manipulate host immune responses is largely unknown.In other protozoan parasites, including Plasmodium, Entamoeba, Toxoplasma, and Leishmania, a homolog of the mammalian Macrophage Migration Inhibitory Factor (MIF) was identified, which functions as a virulence factor aiding in the establishment or persistence of infection by modulating the host innate immune response 11,12 .Though the sequence identities between mammalian and protozoan MIF only range between 20 and 27%, they share a well-conserved trimeric architecture [12][13][14] .However, whether trypanosomes also contain a MIF-like molecule that facilitates the infection was an outstanding question.
Here, we show that trypanosomes, despite their close relationship to Leishmania, did not harbor a MIF homolog.Instead, we identified Q586B2, a protein that is localized in vesicles and harbors tautomerase activity (an isomerase enzyme catalyzing the interconversion of ketoand enol-groups).A constitutive or inducible deficiency of the Tb927.6.4140gene, coding for Q586B2, does not affect parasite fitness as such, but decreases parasite establishment and prolongs survival of the host, which is mechanistically coupled to its potent IL-10-inducing capacity in myeloid cells.In addition, Q586B2 deficiency increases the propensity of parasites to differentiate into short stumpy forms.We provide evidence that blocking this protein or using it in a vaccination setting holds great promise to hamper trypanosome-mediated diseases.

Results
In silico analysis identified Q586B2 as a novel T. brucei protein exhibiting structural homology with the T. cruzi protein Q4D6Q6 In an attempt to identify a MIF homolog within AT, the Leishmania MIF protein sequence (Q4Q413) was blasted against the available trypanosome genome (Trypanosoma brucei brucei TREU 927) within the Wellcome Trust Sanger Institute database (http://www.sanger.ac.uk/ resources/databases).However, no MIF homolog was detected in trypanosomes.We then screened for proteins with MIF-like characteristics, such as an amino-terminal proline residue immediately following the initial methionine residue and a maximum size of 115 amino acids, by using the first 25 amino acids of the Q4Q413 protein for blasting.This approach identified Tb927.6.4140, a gene located on chromosome 6, coding for a hypothetical unknown protein (Q586B2) with an expected size of 13 kDa (348 bp, 115 aa), and little sequence similarity with MIF.In accordance, the Protein Homology/analogy Recognition Engine Version 2.0 (Phyre 2 ) (http://www.sbg.bio.ic.ac.uk/ phyre2/html/page.cgi?id=index), that also analyzes structural homologies between proteins, yielded no homology between Q586B2 and MIF.However, Phyre 2 predicted a weak probability of homology (16% sequence identity and 51.5% structural homology confidence for the first part of the protein, ranging from amino acid 13-70) with 4-oxalocrotonate tautomerase (4-OT) of the Archean species Archaeoglobus fulgidus (Fig. 1a, upper panel).Phyre 2 also uncovered a high homology to Q4D6Q6 15 , a conserved kinetoplastid-specific protein from Trypanosoma cruzi with unknown function (55% sequence identity and 100% structural homology confidence) (Fig. 1a, lower panel).Using ColabFold 16,17 , the monomeric structure of Q586B2 was predicted and compared to the known structure of monomeric Q4D6Q6 (Fig. 1b(i)).In this algorithm, each residue receives a local confidence measure on a scale from 0 to 100 (i.e., the pLDDT or predicted local distance difference test score value; 100 representing the highest confidence).The prediction of the Q586B2 protein structure shows pLDDT values higher than 90 for 86 out of 115 amino acid residues (Fig. 1(i), (ii)).Loop regions show a slightly lower confidence, but the pLDDT is still higher than 70.The level of confidence is only low (pLDDT between 50 and 70) for residues 42-46, which are part of loop 3 between α1 and β3 (highlighted in yellow).Regions with a low pLDDT may be unstructured, either intrinsically disordered or structured only in the context of a larger complex.As shown in Fig. 1b(i), the Q586B2 monomer has a high structural homology with monomeric Q4D6Q6 15 , for which the crystal structure (PDB code 6xyb) showed loop 3 to be unstructured.
As Q4D6Q6 reportedly forms a homo-tetramer 15 , we used Colab-Fold to predict the likelihood of Q586B2 to organize into a similar multimeric structure (Fig. 1c).The Predicted Aligned Error (PAE) plot shows that ColabFold is confident about the relative domain positions (Fig. 1c, left panel).The color at (x, y) indicates the expected position error at residue x if the predicted and true structures were aligned on residue y.The low PAE (colored blue) along the diagonal indicates a correct prediction of the monomeric structures (A-D), whereas a low error along the different axes of the PAE plot indicates a correct relative orientation of the monomers in the oligomeric structure (A-D).As shown from the overlay with the Q4D6Q4 tetrameric structure (Fig. 1c, right panel), Q586B2 is predicted to form a very similar propeller-like homo-tetramer, consisting of 4 monomers that adopt a ββαββαββ topology 15 .
Collectively, these data demonstrate that trypanosomes do not harbor a bona fide MIF homolog, but uncovered Q586B2 as a protein with structural homology to the T. cruzi protein Q4D6Q6.

Q586B2 is an evolutionary conserved protein within the related families Trypanosomatidae and Bodonidae
To chart the taxonomic distribution of proteins homologous to Q586B2 and Q4D6Q6, thereby elucidating the evolutionary origin of the underlying gene family, we used tBLASTn searches to screen a wide range of eukaryote genomes in the NCBI and TriTrypDB databases.These searches uncovered the presence of three paralogous genes (Tb927.6.4140,Tb927.2.2770, and Tb10.26.0680) in T. b. brucei as well as in the (sub)species T. b. gambiense, T. congolense, T. equiperdum, T. evansi, and T. vivax.A fourth paralogous gene was discovered in four other Trypanosoma species (T.cruzi, T. rangeli, T. conorhini, T. Theileri), as well as in all investigated species of the trypanosomatid genera Leishmania, Angomonas, Crithidia, Endotrypanum, Herpetomonas, Leptomonas, Lotmaria, Paratrypanosoma, Phytomonas and Strigomonas, and in Bodo saltans, a free-living (non-parasitic) species of the closely related family Bodonidae 18 (Table S1).Remarkably, BLAST searches did not reveal any related genes in aquatic Trypanosoma species.In addition, no genes homologous to Tb927.6.4140(encoding Q586B2) were found in any kinetoplastid genome outside Bodonidae and Trypanosomatidae, nor in those of any other eukaryote supergroup.This pattern of occurrence suggests that the gene family to which Tb927.6.4140belongs arose in a direct ancestor of the Bodonidae and Trypanosomatidae sister families.Comparative alignment shows that the different proteins encoded by the closest related genes found in other trypanosomatid and bodonid taxa share considerable sequence similarity to Q4D6Q6 and Q586B2 (Fig. S1a).Moreover, Phyre 2 analyses predict with high confidence that the majority of them share the same secondary structure and folding pattern as Q4D6Q6, which for all proteins was selected by Phyre 2 as the folding template.
To investigate the evolutionary history of the genes encoding Q586B2 and its homologs, we conducted a Bayesian phylogenetic analysis based on an alignment of 126 gene sequences retrieved from the abovementioned taxa (Fig. S2).The resulting tree is composed of four major gene clades, each including one of the four paralogues found in most species.This pattern indicates that a single ancestral gene underwent a series of duplication events prior to the divergence of Bodonidae and Trypanosomatidae, yielding a gene family of four paralogues that remained stable in most taxa.The presence of only three paralogues in T. b. brucei and closely related (sub)species is explained by a loss of one of the four paralogues within Trypanosoma.The genes encoding Q4D6Q6 (TcCLB.511733.90)and Q586B2 (Tb927.6.4140) are found deeply nested in one of these four gene clades (Fig. S2), explaining the presence of orthologous genes in all analyzed trypanosomatid genera as well as in Bodo saltans.The presence of orthologous genes in a broad taxonomic range of Trypanosomatidae as well as in their sister family Bodonidae implies that the ancestral gene of Tb927.6.4140arose before the onset of a parasitic lifestyle and long before the independent evolution of dixenous life histories in multiple trypanosomatid lineages (Fig. S1b).

The Q586B2 protein is present within intracellular vesicles and at different life cycle stages, being conserved among trypanosomatids of the Kinetoplastida phylum
To obtain functional insights in the Q586B2 protein, we produced the protein recombinantly in E. coli WK6 cells by codon optimizing the Tb927.6.4140gene and cloning it with an N-terminal His 6 -and FLAGtag, allowing the removal of the tag after purification.Upon Q586B2 purification via IMAC and size exclusion chromatography, a uniform peak of approximately 55 kDa was observed, suggesting that the protein forms a tetramer (Fig. S3a).Since part of the Q586B2 protein showed resemblance to 4-OT (Fig. 1a), which belongs to the tautomerase superfamily, we first assessed its putative tautomerase activity.Q586B2 indeed exhibited a moderate tautomerase activity, which is significantly lower than the prototypical tautomerase activity of MIF (Fig. S3b).Moreover, in contrast to MIF, the Q586B2 tautomerase activity was not inhibited by the small molecule ISO-1, suggesting structural differences in the active site (Fig. S3b).This is in line with the fact that the predicted crystal structure of Q586B2 is a tetramer (see Fig. 1c), while MIF is a trimer.
To create novel research tools, we used the recombinant Q586B2 protein for the generation of nanobodies (Nbs), which are the antigen-recognition domains of camelid heavy chain-only antibodies.Following immunization of a llama and screening of its Nb repertoire for specificity against the recombinant Q586B2 protein, 8 distinct Nbs belonging to 8 different families based on the difference in their CDR3 regions (Fig. S4a(i), highlighted in yellow) could be retrieved, and after purification found to have nM affinity for binding to Q586B2 (Fig. S4a(ii)).Using flow cytometry, these Nbs were shown to recognize their target only on fixed and permeabilized parasites, suggesting that Q586B2 is localized intracellularly (Fig. S4b, left panels).Nb39 consistently gave the highest Q586B2 detection signal (Fig. S4b, right panel) and will be used throughout this manuscript, while Nb27 was unable to detect native Q586B2.Immunofluorescence microscopy using Nb39 confirmed that Q586B2 was localized in vesicles dispersed throughout the whole parasite (Fig. 2a).More specifically, Q586B2 partially co-localized with the glycosomal glycerol kinase (GK) and the vacuolar iron transporter (VIT1) present in acidocalcisomes, with RAB5B and RAB11 present in early and recycling endosomes, respectively, and with RAB2B as a marker of the early exocytosis compartment (Fig. 2b).No colocalization with the lysosomal marker p67 nor endoplasmic reticulum marker BiP was observed.
Interestingly, Q586B2 was not only expressed in the bloodstream stage (Figs.S4b and 2a, b), but also in the procyclic (i.e., early forms present in the fly midgut following a bloodmeal) and metacyclic (i.e., forms present in the salivary glands of the fly preadapted for infection into the mammalian host; purified from Glossina morsitans morsitans salivary glands) stages of T. b. brucei (Fig. 2c(i)), indicating that it is constitutively expressed throughout the entire parasites' life cycle.Besides T. b. brucei, both the human pathogens T. b. gambiense and T. b. rhodesiense harbor this protein, as well as T. evansi, T. congolense and T. vivax (Fig. 2c(ii)), indicating that it is highly conserved among trypanosomes.These data also demonstrate that Nb39 recognizes a widely conserved epitope within the Q586B2 protein.Other trypanosomatids belonging to the phylum Kinetoplastida were also suggested to harbor a paralogous gene (Fig. S2).Using Nb39, we demonstrated the expression of a homologous protein in T. cruzi, Leishmania major, and L. infantum species (Fig. 2c(iii)), albeit at a lower level than in trypanosomes (Fig. 2c, right panel).Conversely, Q586B2 was not present in the parasitic protist P. falciparum belonging to the phylum Apicomplexa.
Together, Q586B2 was shown to be present in most trypanosomatids belonging to the phylum Kinetoplastida, being localized in intracellular vesicles and expressed throughout the T. b. brucei parasite's life cycle.Tb927.6.4140-deficiencyattenuates first-peak parasitemia, leading to a prolonged survival of the host To study the role of Q586B2 in AT biology, we generated Tb927.6.4140knockout (Tb927.6.4140-KO)T. b. brucei parasites using the 90:13 pleiomorphic strain and a similar approach as described before 19 .PCR confirmed the successful deletion of the gene (Fig. S5a).Tb927.6.4140-KOparasites exhibited a similar in vitro proliferation rate as compared to WT parasites, indicating that the absence of Q586B2 does not affect the parasite's fitness (Fig. 3a).To determine whether Tb927.6.4140deficiencyaffects the parasite's presence within the tsetse fly vector, we investigated midgut infection establishment in Glossina morsitans morsitans flies following feeding on WT or Tb927.6.4140-KOtrypanosome-infected blood.The number of tsetse flies exhibiting a midgut infection, as well as the midgut parasite load, was similar in both conditions (Fig. 3b, left and right panel).Together, these data suggest that Q586B2 is dispensable for parasite fitness in vitro and survival within the tsetse fly vector.
To study whether Q586B2 affects parasitemia in the murine host, C57BL/6 mice were intraperitoneally injected with 5000 WT or Tb927.6.4140-KOT. b. brucei parasites, and infection parameters were Fig. 1 | In silico identification of Q586B2 as a novel T. brucei protein exhibiting structural homology with the T. cruzi protein Q4D6Q6.a Upper panel: The Leishmania MIF protein sequence (Q4Q413) was blasted against the available trypanosome genome and identified Q586B2 as a potential candidate.Phyre 2 (Protein Homology/analogy Recognition Engine V 2.0) analysis predicts a 16% sequence identity and 51.5% confidence of structural homology with 4-OT (4-oxalocrotonate tautomerase, c3m20A) for the first half of the Q586B2 protein.Residues with 100% homology (highlighted in gray), alpha helices (green), and beta strands (blue).Lower panel: Phyre 2 analysis predicts a high homology of Q586B2 to Q4D6Q6 with 55% sequence identity and 100% structural homology confidence.Green helices represent α-helices, blue arrows indicate β-strands and faint lines indicate coils.The 'SS confidence' line indicates the confidence in the prediction (red: high confidence, blue: low confidence).b Upper panel: ColabFold predictions (https://www.alphafold.ebi.ac.uk) of the structure of Q586B2 (left) as a monomer, based on the Per-residue confidence (pLDDT: i.e., a predicted local distance difference test score), which is a per-residue measure of local confidence on a scale from 0 to 100.Regions with high (dark blue) and low pLDDT (yellow, these may be unstructured; either intrinsically disordered or structured only in the context of a larger complex).The crystal structure of the Q4D6Q6 monomer is shown on the right (pdb code 6xyb).c Left panel: Predicted Aligned Error (PAE) plot.The color at (x, y) indicates the expected position error at residue x if the predicted and true structures were aligned on residue y.A low PAE (colored blue) along the diagonal indicates correct prediction of the monomer structures, whereas a low error along the different axes of the PAE plot indicated correct relative orientation of the monomers in the oligomeric structure.A-D: the different monomers represented as consecutive residue numbers.Right panels: the ColabFold prediction of the Q586B2 structure and the crystal structure of Q4D6Q6 show a similar propeller-like tetramer consisting of 4 monomers that adopt a ββαββαββ topology, and overlay of the documented tetrameric structure of Q4D6Q6 15 with the predicted tetrameric structure of Q586B2 (pink).monitored.Tb927.6.4140-KOT. b. brucei-infected mice exhibited a significantly lower first-peak parasitemia (~80% reduction) and a significantly prolonged survival time (median survival time WT-infected mice: 22 ± 4 days vs. Tb927.6.4140-KO-infectedmice: 56 ± 20 days) (Fig. 3c, d).The prolonged survival observed in Tb927.6.4140-deficientparasite-infected mice correlated with a significantly reduced ATassociated immunopathology.Indeed, compared to WT parasite-infected mice, Tb927.6.4140-deficientparasite-infected mice exhibited reduced anemia as well as serum ALT (liver-specific injury marker) and AST (more general tissue injury marker) levels at day 6 p.i. (acute phase) and day 22 p.i. (chronic phase), suggesting a lower immunopathology (Fig. S6).A possible explanation could be that due to the lower parasite levels observed in the Tb927.6.4140-deficientparasiteinfected mice at the early stages of infection, less parasite-derived PAMPs (i.e., VSG, CpG,…) are released that in turn will trigger a reduced inflammatory immune response in infected mice 20 .To delineate the exact time window within which Q586B2 functionality is important, we generated doxycycline-inducible Tb927.6.4140-knockdown(Tb927.6.4140-KD)T. b. brucei parasites using the 90:13 pleiomorphic strain.In vitro culture of inducible Tb927.6.4140-KDparasites in the presence of 1 µg/ml doxycycline is causing a full knockdown of the Q586B2 protein within 48 h of doxycycline exposure (Fig. S5b).Based on these data, WT or Tb927.6.4140-KDparasites were cultured for 48 h in presence of doxycycline to ensure full knockdown of the Q586B2 protein upon inoculation and subsequently injected into C57BL/6 WT mice that were also administered doxycycline in the drinking water already 2 days prior to infection to ensure a systemic presence of the antibiotic at the start of infection.As an internal control for each parasite strain, mice were given drinking water without antibiotic.While doxycycline administration did not affect the first-peak parasitemia in WT parasite-infected mice, it significantly lowered this infection parameter in mice infected with Tb927.6.4140-KDparasites (Fig. 3e), phenocopying infections with full Tb927.6.4140-KOparasites (Fig. 3c).Along the same line, the inducible Tb927.6.440-KD-infectedmice survived significantly longer than the WT control group (WT: 24 ± 3 days, Tb927.6.4140-KD:34 ± 5 days) (Fig. 3f).Of note, mice infected with Tb927.6.4140-KDparasites that received normal drinking water showed a similar parasitemia and survival time (WT: 23 ± 2 days, Tb927.6.4140-KD:24 ± 3 days) as WT control mice (Fig. 3c, d, respectively).Next, mice were given doxycycline starting from 3 days postinfection (Fig. 3g).Under these conditions, the effect on first-peak parasitemia and survival (Fig. 3g, h) in Tb927.6.440-KD-infectedmice was abrogated, indicating that Q586B2 exerts its biological effect within the first 3-4 days of infection.
Collectively, these data illustrate that Q586B2 plays a crucial role in the early stages of T. b. brucei infection (before peak parasitemia), allowing a more efficient initiation of infection.

Q586B2 deficiency promotes early inflammatory responses in vivo and stimulates short stumpy differentiation at peak parasitemia
To mechanistically understand the role of Q586B2 during the early stages of T. b. brucei infection, Tb927.6.4140-KO, and WT parasites were injected intraperitoneally and 18 h later the CD45 + immune cell composition within the peritoneum was analyzed via flow cytometry (Fig. 4a, and Fig. S7 describing the gating strategy).Within the CD45 + hematopoietic compartment, only the relative presence of macrophages and PMNs was significantly increased in both WT and Tb927.6.4140-KOparasite-infected mice as compared to naïve mice (Fig. S8a).Notably, no significant differences in the peritoneal CD45 + immune cell composition were detected between WT and Tb927.6.4140-KOparasite-infected mice.While both Tb927.6.4140-KO and WT parasite-infected groups showed a drop in the number of peritoneal cells as compared to non-infected animals, significantly less cells were recovered from mice that received Tb927.6.4140-KOparasites (Fig. 4b).This excessive drop in peritoneal cell numbers in Tb927.6.4140-KOparasite-infected mice was especially reflected in significantly reduced macrophage numbers (Fig. 4b).
A reduction in peritoneal cell numbers, in particular macrophages, has previously been referred to as the macrophage disappearance reaction (MDR) 21 , which is indicative of an ongoing local inflammation.Hence, these data suggest that Tb927.6.4140-KOparasites may induce a stronger peritoneal inflammation compared to WT parasites.18 h post-infection, cytokine levels were still very low in the peritoneal fluid, except for MIF, which is known as an inflammatory driver cytokine in AT 22 .Interestingly, Tb927.6.4140-KOparasites induced higher MIF levels, corroborating the notion of a stronger peritoneal inflammation at very early stages of infection (Fig S8b).Of note, a similar observation was observed in the serum.To test this assumption further, equal numbers of peritoneal cells from Tb927.6.4140-KO and WT parasite-infected mice were cultured, and inflammatory cytokine production was measured.Key inflammatory cytokines, such as TNF, IL-6, and MIF, all of which were documented to play an important role during AT [22][23][24] , were produced at higher levels by peritoneal cells from Tb927.6.4140-KOparasite-infected animals compared to WT parasite-infected animals (Fig. 4c).Conversely, the anti-inflammatory cytokine IL-10 was produced less by these peritoneal cells (Fig. 4c).
An alternative or complementary explanation for the reduced peak parasitemia of KO parasite-infected mice could be a higher propensity of these parasites to differentiate into short stumpy forms.To assess this, WT and Tb927.6.4140-KOparasites were purified at peak parasitemia and PAD1 expression, a protein only present in stumpy form parasites, was determined via western blot.Tb927.6.4140-KOparasites expressed higher PAD1 levels (Fig. S5c), suggesting that Tb927.6.4140regulates short stumpy differentiation.
In conclusion, the absence of Q586B2 results in an enhanced inflammatory environment at the site of infection and promotes short stumpy differentiation at peak parasitemia.

Q586B2 is a secreted protein that is able to induce early IL-10 secretion by myeloid cells in vivo, promoting T. b. brucei infection onset
The immunomodulatory effect of Q586B2 may be due to a direct effect on inflammatory cells, provided that this protein is secreted by the parasites.To test this possibility, we developed a Nb39-based sandwich ELISA that was able to detect recombinant Q586B2 in solution (Fig. 5a).Interestingly, Q586B2 was detected in the culture medium (secretome) of in vitro cultured WT parasites, while no signal was obtained for the secretome of Tb927.6.4140-KOT. b. brucei parasites, indicating that Q586B2 is indeed released by WT parasites (Fig. 5b).Importantly, Q586B2 could also be detected in the serum of T. b. brucei-infected mice as early as 6 days post-infection (Fig. 5c), further supporting the conclusion that Q586B2 is released during the early infection phase.
Macrophages are central regulators of inflammation 25 , so we next assessed the direct effect of Q586B2 on these cells' ability to secrete proand anti-inflammatory cytokines (Fig. 5d).A 48 h in vitro culture of bone-marrow-derived macrophages (BMDMs) with different concentrations of endotoxin-free recombinant Q586B2 protein resulted in a prominent induction of IL-10 secretion by even the lowest Q586B2 concentration, while this was not observed for TNF, IL-6 or MIF.TNF and IL-6 are, however, induced by higher Q586B2 concentrations.Of note, the MIF production by BMDMs stimulated with buffer alone is high, which is in line with findings that BMDMs produce nanogram amounts of MIF upon culturing 26 .Even at intermediate and high Q586B2 concentrations, the level of IL-10 induction was superior to the onset of various inflammatory cytokines (IL-10: 8.4-fold induction at 0.250 µg/mL Q586B2; TNF: 2.9-fold induction; IL-6: 2.6-fold induction; MIF: 1.5-fold induction).Noteworthy, similar results were observed using peritoneal macrophages (Fig. S9).These data suggest that Q586B2 potently induces an anti-inflammatory IL-10-driven response by macrophages.
To assess whether Q586B2 is effectively able to trigger IL-10 production in vivo, we employed IL-10 reporter (VerT-X) mice.18 h after the intraperitoneal injection of 5 µg endotoxin-free Q586B2, IL-10 production was mainly induced in resident large peritoneal macrophages (LPM) (ΔMFI as compared to PBS control = 255.2) and neutrophils (ΔMFI as compared to PBS control = 240,3) (Fig. 5e and Fig. S7 for the gating strategy of LPM).To test whether the macrophage/ neutrophil-derived IL-10 in response to Q586B2 was important to regulate early infection parameters, LysMCre × IL-10 fl/fl mice, in which IL-10 is conditionally ablated in macrophages and neutrophils, were infected with WT or Tb927.6.4140-KOT. b. brucei parasites.Interestingly, no difference in first-peak parasitemia was observed between these two groups of mice (Fig. 5f), in contrast to the reduced peak parasitemia observed in WT mice following infection with Tb927.6.4140-KOparasites (Fig. 3c).These results highlight the crucial role of Q586B2-induced myeloid-derived IL-10 to dampen parasitemediated host inflammation, which in turn promotes first-peak parasitic burden.
Collectively, T. b. brucei parasites employ Q586B2 to trigger early IL-10 production by myeloid cells at the site of infection, which promotes a higher first-peak of parasitemia.

Q586B2 as a potential therapeutic target for trypanosomatids
Since Q586B2 is produced early during the infection and promotes parasite establishment, this protein could be considered as a therapeutic target.Therefore, the panel of anti-Q586B2 Nbs was tested for their ability to block the Q586B2-mediated IL-10 induction in macrophages.To this end, BMDMs were incubated with 1 µg/ml Q586B2 in the presence of 5 µg/ml anti-Q586B2 Nb.Several Nbs were found to significantly inhibit the IL-10-inducing potential of Q586B2, with Nb32, Nb41, and Nb58 being the most potent ones (Fig. 6a).To assess whether these Nbs could affect the infection parameters in vivo, WT mice were injected twice daily with 1 mg Q586B2-blocking or non-blocking Nbs, starting from day 0 until day 4 post-T.b. brucei infection.As shown in Fig. 6b, mice treated with a mixture of Q586B2-blocking Nbs (Nb32 and Nb58) exhibited a significantly reduced first-peak parasitemia compared to mice that received a mixture of non-blocking Nbs (Nb39 and Nb53).This Nbmediated reduction of peak parasitemia is comparable to the phenotype observed in Tb927.6.4140-KO and doxycycline-inducible Tb927.6.4140-KDT. b. brucei parasite-infected mice.Moreover, mice treated with the Q586B2-blocking Nbs survived significantly longer than the control group treated with non-blocking Nbs (Fig. 6c) (median survival time Q586B2-blocking Nb treated: 38 ± 2 days vs non-blocking Nb treated: 32 ± 2 days (p-value: 0.01)).These data provide additional evidence that the Q586B2 protein promotes early parasite establishment and propose anti-Q586B2 Nbs as potential therapeutic tools to better control this early infection phase.

Immunization with Q586B2 attenuates peak parasitemia and prolongs survival in different homologous and heterologous trypanosome models
We next tested whether a Q586B2 immunization could modulate the host response towards the parasite.Recombinant Q586B2 was intraperitoneally (i.p.) injected in the presence of Complete Freund/ Incomplete Freund adjuvant, twice at 3 weeks intervals (referred to as Q586B2 immunization), and the subsequent effect on infection parameters was assessed.The control (mock-treated) group included animals receiving only the adjuvant.The Q586B2 immunization triggered a strong humoral immune response against this protein, as demonstrated by the ability of purified IgG antibodies to detect the native Q586B2 protein in parasite lysate via ELISA (Fig. S10a, b).Interestingly, upon challenge (10 days post last dose) with WT T. b. brucei parasites, defined as a homologous setting, Q586B2 pre-treated mice showed a significant reduction in first-peak parasitemia and a prolonged survival (mock-treated: 32.6 ± 3.2 days versus Q586B2-treated: 42.4 ± 4.4 days) (Fig. 7a, b).
Considering the high conservation of this protein family within trypanosomatids, the Q586B2 immunization strategy was evaluated for its cross-protective effect against other trypanosomatid species.Interestingly, Q586B2-immunized mice also showed a reduced firstpeak parasitemia upon challenge with T. congolense (Tc13) and T. evansi, resulting in a significantly prolonged survival (Tc13: mocktreated: 82.4 ± 8.0 days versus Q586B2-treated: 107.6 ± 18.6 days and T. evansi: mock-treated: 16.4 ± 5.3 days versus Q586B2-treated: 25.2 ± 6.2 days) (Fig. 7c-f).Interestingly, the heterologous pretreatment with Q586B2 also conferred partial protection against one of the most aggressive strains of American trypanosomes, as evidenced by the reduced parasitemia and increased survival of pretreated mice infected with the aggressive RA strain of T. cruzi (Fig. 7g, h).
Together, these results show that an immunization of naïve mice with the T. b. brucei protein Q586B2, emulsified in adjuvant, partially protects against a broad range of trypanosomatids.

Discussion
Parasites acquired elaborate mechanisms to escape the host immune response, allowing parasite establishment and persistence.In this context, many parasites express a MIF homolog that is able to thwart and counteract the mammalian MIF, thereby diminishing MIFmediated inflammation and elimination by the host immune response.Remarkably, no MIF homolog was found in the genome of African trypanosomes.However, these parasites express an uncharacterized protein, annotated as Q586B2, that presents some MIF-like characteristics, such as a tautomerase activity.Q586B2 was expressed throughout the entire life cycle of T. brucei.This is in line with the work from Fergusson et al. 27 , who performed a genome-wide comparative proteomic analysis of bloodstream and procyclic T. brucei parasites Fig. 3 | Tb927.6.4140-deficiencydoes not affect in vitro parasite proliferation and establishment in tsetse flies but attenuates first-peak parasitemia and prolongs survival in the mammalian host.a In vitro parasite growth of either WT (90:.13 strain) or Tb927.6.4140-deficientT. b. brucei parasites cultured for 10 days in HMI-9 medium (starting at 5 × 10 4 parasites/ml).Results are representative from 3 independent experiments, whereby for each clone and time-point duplicate samples (n = 2) were analyzed and data are presented as means ± SEM. b Number of G. m. morsitans tsetse flies with a midgut infection (procyclic forms, red bars) or with no infection (grey bars).In total, per group, 16 flies fed on blood containing either WT parasites or Tb927.6.4140-deficientparasites were evaluated for a midgut infection 10-12 days post-feeding (left panel) and parasite levels were determined via RT-PCR (right panel).NS Not significant.c, d Parasitemia and survival of C57BL/6 mice infected with Wild-type (WT: T. b. brucei parasite 90:13 pleiomorphic strain, black circles) or Tb927.6.4140-deficient(red circles) parasites.Right panels: e, f Parasitemia and survival of C57BL/6 mice infected with Wild-type (WT: T. b. brucei 90:13 pleiomorphic parasite strain, black boxes), doxycycline-treated WT parasites (white boxes) or Tb927.6.4140-knock-down(KD) (blue circles) and doxycycline-treated Tb927.6.4140-KD(red circles) parasites.Mice were treated with doxycycline in the drinking water 2 days before initiating the infection.g, h Parasitemia and survival of C57BL/6 mice infected with WT parasites treated with doxycycline 2 days prior to infection (black boxes) or 3 days post-infection (white boxes) as well as Tb927.6.4140-KDparasites treated with doxycycline 2 days prior to infection (red circles) or treated after 3 days post-infection (blue circles).Results are representative of 2-3 independent experiments (n = 5) and presented as mean (c, e, g) or median (b, d, f, h) ± SEM.Parasitemia was analyzed using a Multiple paired t-test (only significance p < 0.05 is shown) and survival curves were analyzed via a Mantel-Cox test (MS median survival).Of note, statistics on parasitemia were performed by comparing doxycycline-treated WT and Tb927.6.4140-KDparasites.Source data are provided as a Source Data file.and proposed this protein to be present in both life stages.Fergusson et al. annotated it as "putative paraflagellar rod protein" in the GeneDB (https://www.sanger.ac.uk/tool/genedb/), but no attempts were made to functionally characterize this protein.Our genomic screening and subsequent phylogenetic analysis revealed that this is an evolutionary conserved protein within trypanosomatids, including T. cruzi and Leishmania spp., for which there exist 4 paralogues.Since no homologous genes were found in any other eukaryote taxon, this gene family probably did not originate much earlier than the last common ancestor of Bodo saltans and Trypanosomatidae, which lived an estimated 700-460 million years ago 18,28 .This finding has implications for understanding the role of this protein family in the light of parasite evolution and the related adaptation of controlling a host's immune system.First, the lack of homologous proteins outside this clade explains why no Q586B2-related protein was observed in Plasmodium falciparum, as this parasite descended from a very different earlydiverged eukaryote lineage.Plasmodium belongs to the Apicomplexa phylum within the "SAR" supergroup of eukaryotes, while Trypanosomatidae are Euglenozoa within the supergroup Discoba, a clade of 'excavate' eukaryotes supported by phylogenomic data 18,29 .Second, since the Bodo saltans sequences are scattered across several of the major gene clades, the duplication events that gave rise to the four paralogous genes in most taxa must have happened before the divergence of Bodo saltans and Trypanosomatidae.Consequently, diversification of this gene family most likely happened prior to the origin of an obligatory parasitic lifestyle in Trypanosomatidae.It is now generally accepted that parasitism arose after the divergence of trypanosomatids from free-living flagellates including Bodo saltans, but prior to the basal divergence between Paratrypanosoma confusum and all other trypanosomatids 18,[30][31][32] .We hypothesize that this protein family originally evolved to serve a currently unknown function unrelated to parasitism.Only later, at least one of these paralogues (represented by Q586B2 in T. b. brucei) seems to have acquired a role in the regulation of the host's immune system.This scenario represents a case of evolutionary exaptation, in which one or several proteins evolved a new function alongside a previously existing one.For example, while our study demonstrated a regulatory effect of one member of this protein family on the mammalian immune system, thereby confirming their role in dixenous species during early mammalian host infection, it would be interesting to assess whether members of this protein family are similarly involved in the infection of insect hosts and, thus, may have been recruited earlier to serve an immunomodulatory function in a monoxenous ancestor.
Further analysis demonstrated that Q586B2 was present intracellularly within different vesicles.Indeed, co-localization experiments revealed that Q586B2 partially colocalizes with glycosomes, which is in line with the work by Colasante et al. 33 , who identified this protein via nanoLC/MS in the proteome of glycosomes isolated from bloodstream and procyclic T. b. brucei parasites that were cultured in vitro.Likewise, Q586B2 was identified as a glycosomal protein within the proteome of procyclic T. brucei parasites via epitope-Tag Organelle Enrichment and SILAC quantitative Proteomics [34][35][36] .The co-localization experiments further revealed that Q586B2 was also present within acidocalcisomes as well as in early endocytic and recycling vesicles.The presence of Q586B2 within acidocalcisomes could be due to its interaction with Q389K9 (Tb927.10.11300), an interaction that was identified via yeasttwo-hybrid screening (https://www.ebi.ac.uk/intact/search?query=id: Q586B2*#interactor). Q389K9 was indeed shown to be present within acidocalcisomes 37 .The presence of Q586B2 in endocytic and recycling vesicles suggests that Q586B2 can be released, which is in line with our Nb-based ELISA data showing Q586B2 presence in the medium or serum of infected animals.
To acquire more insight into the biological role of Q586B2, we generated Tb927.6.4140-deficientparasites.These parasites were fully viable without any effect on cell proliferation in vitro and establishment within the midgut of tsetse flies, suggesting that Q586B2 is not essential for parasite survival.Our data further demonstrate that the early presence of Q586B2, within the first three-four days post-infection, is required to attenuate the host inflammatory response, thereby allowing efficient parasite establishment and dissemination within its host.Moreover, IL-10 produced by large peritoneal macrophages and/ or PMNs was shown to be instrumental in the Q586B2-mediated parasite establishment.Our in vitro data demonstrate that Q586B2 can induce both pro-as well as anti-inflammatory cytokines, yet it exhibits a more potent effect on IL-10 production, at least in macrophages, via an as yet unknown mechanism.These results are in line with the work of Caljon et al. 38 , showing a rapid induction of IL-10 (within the first 18-90 h post-infection) and a decline of the inflammatory response at the site of infection (i.e., the skin) using a natural tsetse fly-mediated infection route.Furthermore, upon skin damage, Tim4 + /CD206 + dermal macrophages were reported to produce IL-10, which in turn allows the survival and maintenance of anti-inflammatory Tim4 + /IL-10 + macrophages 39 .Hence, the T. b. brucei-derived Q586B2 protein appears to be a key mediator in triggering this early IL-10 production by myeloid cells and thereby limiting excessive early inflammatory responses in favor of parasite establishment.This places Q586B2 in a series of T. brucei-derived proteins, including the T. brucei Kinesin Heavy Chain 1 (TbKHC1) and T. brucei Adenylate Cyclase (TbAdC), that can sculpt a more permissive environment for parasite survival 9,19,40 .However, the reduced peak parasitemia in Tb927.6.4140-deficientparasite-infected mice could also be partially due to the fact that these parasites are more prone to differentiate into short stumpy forms, as evidenced by their higher PAD1 expression.Hence, Q586B2 could play a dual role during the early stages of infection, whereby on the one hand it could prevent excessive host inflammation essential for parasite establishment, and on the other hand prevent excessive short stumpy differentiation thereby allowing first-peak development.Hence, it is tempting to speculate that Q586B2 could serve as a parasite sensor or regulator for quorum sensing in favor of parasite establishment.
Since Q586B2 is highly conserved amongst trypanosomatids of the Kinetoplastida phylum (including medically important parasite species such as T. brucei, Leishmania, and T. cruzi), and an important virulence factor, we considered it as a potential therapeutic target.In this context, we identified a series of anti-Q586B2 Nbs that could prevent the protein's IL-10-inducing capacity and that could reduce first-peak parasitemia and prolong survival when administered to the infection site shortly after parasite inoculation.Of note, the effect on survival of using blocking Nbs is less pronounced than the effect of knocking-out the gene in T. brucei, which could be due to the rapid clearance rate of the Nbs and/or the fact that treatment was halted after 4 days post-infection.However, the pharmacokinetics of the Nb constructs could be improved by reformatting them into a half-life extended format 41 or, alternatively, by injecting the Nbs for a longer period of time (post-peak parasitemia).This passive immunization, though interesting, requires insight in the timing of the bite by a parasite-infected tsetse fly, considering that the Nb-based Q586B2 blockade only works in the earliest stages after infection.Hence, we considered an immunization with Q586B2, which was indeed found to attenuate peak parasitemia and prolong host survival.It is important to realize that the effect on survival in this setting, though significant, may be hampered by the fact that these parasites are able to escape elimination via migration into tissues (dermis, fat tissue, brain) that are less accessible to antibodies 42 .Nevertheless, its Fig. 4 | Tb927.6.4140 is essential to attenuate early-stage cellular immune responses within the peritoneum and Tb927.6.4140-deficiencyinduces a stronger anti-inflammatory response within the peritoneum in vitro.Mice were injected intraperitoneally with PBS (control), 5000 WT (90:13 strain) parasites, or 5000 Tb927.6.4140-KOparasites, and 18 h later mice were sacrificed, and the peritoneal cell numbers were determined.Of note, all injections consisted of 200 µl.The cellular composition of the peritoneal cells, i.e., B-cell, eosinophils, macrophages, monocytes, PMN (polymorphonuclear cells), CD4 + and CD8 + T cells, and NK cells, was determined using the gating strategy described in Fig. S5.
a Percentages of immune cells withing CD45 + cells in naïve, WT and Tb927.6.4140-KOparasite-infected mice.b Absolute numbers of total peritoneal cells as well as different immune cell subsets in naïve (control), WT, and Tb927.6.4140-KOparasiteinfected mice.c Production of cytokines (TNF, IL-6, MIF, and IL-10, respectively) by PECs (cultured at 2 × 10 5 cells/well) was determined following 48 h incubation.Results are representative of 2 independent experiments (n = 3 for the control and n = 5 for the infected groups) and presented as mean ± SEM.A one-way ANOVA with Turkey's multiple comparison test was performed.NS not significant.partially protective effect is not only observed in a homologous but also in a heterologous setting, with cross-protection against T. congolense and T. evansi.These findings resemble our previously reported GPI-based immunization approach, where also crossprotection against heterologous parasites was obtained 43 .Interestingly, this prophylactic Q586B2 immunization also conferred partial cross-protection against an aggressive strain of T. cruzi.This is to our knowledge the first time that a conserved molecule was identified in trypanosomes with the ability to induce cross-protection against heterologous parasites as well as against different trypanosomatids.Hence, our findings could pave the way to develop a broad-spectrum intervention strategy for human diseases such as HAT, leishmaniasis, and Chagas disease as well as veterinary and economically relevant diseases such as nagana.

Ethics statement
All experiments complied with the ECPVA guidelines (CETS n°123) and were approved by the VUB Ethical Committee (Permit Number: 17-220-02).The protocol of animal procedures followed in this study was approved by the Ethics Committee of Animal Experiments of the Universidad Nacional de San Martín (CICUAE N°14/2022).Mice were monitored daily.Humane endpoints were used during the study, based on weight loss, animals with >25% weight loss were sacrificed using carbon dioxide treatment.
For T. cruzi infections, BALB/cJ male mice (60-day-old) were immunized as described above.Immunized, mock immunized, and naïve control animals were challenged i.p. with 50 parasites from the RA strain 46 , routinely maintained by serial passages in CF1 mice.Parasitemia was tested by sampling blood from the tail tip and counting in a Neubauer chamber.Mice were monitored during 60 days after infection.
For the in vivo Nb treatment experiment mice, wild-type T. b. brucei parasite-infected mice were injected i.p. twice daily with nonblocking anti-Q586B2 Nbs (Nb39 and Nb53) or blocking anti-Q586B2 Nbs (Nb32 and Nb58) (1 mg/mouse/injection) starting from days 0 post-infection and this for 4 consecutive days.Parasitemia at different time points were examined as outlined above.
Parasites were brought at a concentration of 10 7 /ml in PSG buffer, centrifuged at 805 × g (5180R Eppendorf Centrifuge) at room temperature, resuspended in 200 µl BD Cytofix/Cytoperm TM (Fisher Scientific) and kept at 4 °C for 20 min.Finally, the cells were washed with BD Perm/Wash buffer (Fisher Scientific) (1 ml/tube), centrifuged at 805 × g, resuspended in 1 ml Perm/Wash buffer, and stored in the cold room at 4 °C for further analysis (Immunofluorescence microscopy or Flow cytometry).

Immunization, library construction, and screening
Immunization, library construction, and screening were performed as previously described 52 .In brief, Nb libraries were generated by using peripheral blood lymphocytes isolated from a llama that was immunized 6 consecutive times with 100 µg recombinant Q586B2 (purification performed by the VIB Protein Core (VIB, Belgium)) in presence of the same volume of Gerbu adjuvant.The Nb phage library was constructed after total RNA extraction and cDNA subcloning into the phagemid vector pMECS.As soon as enrichment was observed on recombinant Q586B2 (10 μg/ml), individual colonies were selected and transformed into chemo-competent E. coli WK6 cells to allow large-scale purification.Eight distinct Nbs belonging to 8 different families based on the difference in their CDR3 regions could be retrieved and the Nb sequences are shown in Fig. S4a(i).Expression and purification of Nbs (encoding a C-terminal HA-and His 6 -tag) was similar as described earlier 53 .Briefly, following periplasmic extraction, Nbs were purified using immobilized metal affinity chromatography on a His-Trap column (GE Healthcare, Waukesha, WI, USA) and eluted with 0.5 M imidazole (Sigma-Aldrich) in PBS (pH 7.5).The eluate was subsequently purified on a Superdex 75 (10/300) gel filtration column (GE Healthcare) equilibrated with PBS using an Akta Explorer 10S (GE Healthcare using Unicorn 5.1 software).Next, the purity of the Nbs was evaluated via 12% Bis/Tris SDS-PAGE analysis, and the protein concentration determined via Nanodrop (Nanodrop ND-1000, Isogen, Hackensack, NJ, USA).All Nbs were treated with ProSep-Remtox beads (Immu-noSource, Schilde, Belgium) and confirmed to be LPS-free by the Limulus Amebocyte Lysate Kinetic-QCL Kit (Cambrex, East Rutherford, NJ, USA) in accordance with the manufacturer's instructions.Nbs were either used immediately or stored at −20 °C.

Flow cytometry
To analyze the binding of Nbs to fixed and permeabilized parasites, 100 µl of a stock solution of 10 7 parasites/ml were added to the FACS tubes (Falcon).To each tube, 5 µg of HA-tagged Nb was added after which they were incubated on ice for 30 min Of note, an anti-VSG Nb (Nb33) was used as positive control for Nb binding to parasites 50 .The tubes were washed with 200 µl Perm/Wash and centrifuged for 6 min at 805 × g and 4 °C.To each tube, 1 µg of an Alexa Fluor 488 labeled anti-HA IgG (BioLegend) (1/1000 dilution in Perm/Wash buffer) was added after which they were incubated on ice for 20 min.As negative control parasites in presence of the Alexa Fluor 488 labeled anti-HA IgG alone was used.Finally, 200 µl Perm/Wash was added to the tubes and centrifuged for 7 min at 805 × g and 4 °C.The supernatant was removed and the pellet was vortexed after which 150 µl Perm/Wash buffer was added and the samples measured on a FACSCanto TM II flow cytometer (BD).Data were analyzed using FlowJo software 10.

Quantification of cytokines and antibodies
All cytokines except MIF were quantified using the V-PLEX Custom Mouse Cytokine kit (catalog number K152A0H) from Meso Scale Discovery (MSD, Rockville, MD, USA) according to the manufacturer's protocol.Alternatively, the culture medium concentrations of MIF, TNF, and IL-10 (R&D Systems) as well as IL-6 (Pharmingen) were determined by ELISA as recommended by the suppliers.The cytokine levels in peritoneal lavage were determined following concentration (10 times) using Vivaspin concentrators (MWCO-3.5 kDa).Anti-Q586B2 IgGs present in the serum of Q586B2-vaccinated mice were determined via ELISA.Briefly, Q586B2 was coated overnight at 1 μg/ml PBS in 96-well MaxiSorp plates (NUNC).Plates were washed (0.1% Tween 20 in PBS) and blocked (1% BSA in PBS) for 1 h.Next, plates were washed and the sera from individual mice were serially diluted starting from 1/100 in blocking buffer.The ELISA was subsequently performed as described by the suppliers (SBA Clonotyping system-HRP kit (SouthernBiotech, USA)).As negative controls, serum from mockvaccinated mice (a pool of 5 naive mice) was used as well as plates without Q586B2.The OD 450nm recorded on Q586B2-free plates was subtracted from the OD 450nm recorded on the Q586B2-coated plates.To further assess if the IgG present in the serum could recognize specifically the native antigen in parasite lysate, IgGs from pooled mice were purified using protein-G chromatography (ThermoFisher Scientific) as described by the suppliers, the concentration determined spectrophotometrically and then stored at −80 °C till further use.Subsequently, the IgGs were tested in ELISA on coated lysate from T. b. brucei (10 µg/well) prepared as described in Obishakin et al. 56 To this end, following blocking the plate with blocking buffer (1% BSA in PBS), the purified IgGs were ½ serially diluted in blocking buffer starting at 1 µg/ml.The rest of the procedure is the same as for testing the serum in ELISA (see above).

Nb-based Q586B2 ELISA
Nb39 was coated overnight at 5 μg/ml PBS in 96-well MaxiSorp plates (NUNC).Plates were washed (0.1% Tween 20 in PBS) and blocked (1% BSA in PBS) for 1 h.Next, plates were washed and culture medium from in vitro cultured parasites or serum from naïve and infected (day 6 p.i.) mice (diluted 1/2 in blocking buffer) were added.A standard curve was made with recombinant Q586B2 starting at 10 μg/ml (serially diluted ½ in blocking buffer).After 2 h incubation at 37 °C, the plates were washed 5 times with 0.1% Tween 20 in PBS and a biotinylated Nb39 (5 μg/ml in PBS) was used as detecting agent.Two hours later, plates were again washed and streptavidin-HRP was added for one hour.After a final washing, the OD 450nm was recorded and following subtraction of the background signal (wells without Q586B2 added or HMI-9 culture medium alone) the concentration of Q586B2 was determined.
Aspartate aminotransaminase (AST) and alanine aminotransaminase (ALT) measurement Serum AST and ALT levels were determined as described by the suppliers (Sigma-Aldrich).

Anemia determination
Red blood cell (RBC) numbers in blood were determined via hemocytometer by tail-cut (2.5 µl blood in 500 µl RPMI) after which anemia was expressed as percentage of RBCs remaining in infected mice compared to that of non-infected mice.

Statistical analysis
The GraphPad Prism 7 software was used for statistical analyses, Student t-test for paired analyses or one-way ANOVA (with Turkey's multiple comparisons test) or two-way ANOVA; and Log-rank (Mantel-Cox test for survival, using median survival data for comparison).Values are expressed as mean ± SEM, where * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and **** p ≤ 0.0001 are considered significant.

Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.

Fig. 2 |
Fig.2| Q586B2 is expressed in intracellular vesicles throughout different infection stages and in various trypanosomatid species.a Immunofluorescence microscopy showing representative images of purified, fixed, and permeabilized T. b. brucei (AnTat1.1E)parasites stained with dapi (i.e., nucleus) alone or in presence of anti-Q586B2 Nbs (Nb27 and Nb39) or an anti-VSG Nb (Nb33).b Co-localization experiments using fixed V5-tagged T. b. brucei (90:13 strain) parasites stained with different primary antibodies (1:100 α-V5 IgG, 1:1000 α-p67 IgG, 1:1000 α-BiP IgG and 1:50 Nb39), and after washing with fluorochrome-conjugated secondary antibodies.After washing, samples were mounted with ProLong Gold Antifade with DAPI mounting medium.The images were taken using a Zeiss Axio Imager M2 wide field (scale bar: 2.5 µm).Results of 2 independent experiments performed in triplicate are shown in (a) and (b).c Representative flow cytometric anti-Q586B2 Nb binding profile using Nb39 on different purified, fixed, and permeabilized parasites (Gating and conditions as described in Fig.S4b).(i) Parasites used are T. b. brucei bloodstream forms (AnTat1.1E),procyclic forms and metacyclic forms.Signals of parasites without staining (red), parasites in presence of Alexa-488 labeled anti-HA IgG alone (blue), and parasites in presence of Nb39 and Alexa-488 labeled anti-HA IgG (orange) are shown.(ii) Parasites used are T. b. brucei, T. b. gambiense, T. b. rhodesiense, T. evansi, T. congolense, T. vivax, T. cruzi, L. major, L. infantum and Plasmodium falciparum.Signals of parasites in presence of Alexa-488 labeled anti-HA IgG alone was used as negative control (black/gray).c (right panel) Representative dot plot of 3 independent experiments showing the median fluorescence intensity (MFI) of anti-Q586B2 Nb39 following binding to fixed and permeabilized parasites.Each condition consists of technical duplicates (n = 2).

Fig. 5 |
Fig. 5 | Q586B2 is released by parasites, exerts both pro-and anti-inflammatory activity on myeloid cells (BMDMs) in vitro and at the site of inoculation myeloid cells are the most prominent IL-10 producing cells mediating the attenuated first-peak parasitemia observed in Tb927.6.4140-KOparasiteinfected mice.a Schematic of the Nb-based sandwich ELISA whereby Nb39 was used as capturing Nb, biotinylated Nb39 was used as detecting Nbs and streptavidin-HRP was used to develop the ELISA.The illustration in (a) was created using Biorender.comby Maida Živalj.b Detection of native Q586B2 via a Nb-based sandwich ELISA in secretome of WT (90:13 strain) and Tb927.6.4140-KOparasites cultured in HMI-9 for 48 h in vitro.c Detection of native Q586B2 via a Nb-based sandwich ELISA in serum from naïve and T. b. brucei-infected C57BL/6 mice collected at day 6 p.i.For b and c, results are representative for 2 independent experiments (n = 4) and presented as mean ± SEM.Unpaired t-test (Two-tailed) was performed using Welch's correction.d Production of TNF, IL-6, MIF, and IL-10 by BMDMs generated from C57BL/6 mice (2 × 10 5 cells/well) following 48 h incubation with a ½ serial dilution of LPS-free Q586B2 (red bars) starting from 0.25 µg/ml or buffer alone (gray bars).Negative controls: cells incubated with buffer alone or without any protein.Data are shown as means ± SEM and representative of 2 independent experiments (n = 3).A Multiple paired Student's t-tests was used for comparison between experimental groups, with ns indicating not significant for p ≥ 0.05 and p < 0.05.e To assess which cells express IL-10 in vivo, IL-10-GFP (VeRT-X) mice were injected intraperitoneally with PBS (control) or 5 µg LPS-free Q586B2 and 18 h later sacrificed.Subsequently, the cellular source of IL-10 was analyzed via flow cytometry using the gating strategy described in Fig. S5.The expression of IL-10 was presented as delta median fluorescence intensity (delta MFI), by subtracting the MFI of PBS-injected mice from Q586B2-injected mice, for each of the different immune cells that were identified.Results are representative of 2 independent experiments (n = 3) and presented as mean ± SEM.ND Not detected.f Parasitemia of LysMCre × IL-10 fl/fl C57BL/6 mice infected with Wild-type (WT: T. b. brucei parasite 90:13 pleiomorphic strain, black circles) or Tb927.6.4140-deficient(red circles) parasites.Results are representative of 2 independent experiments (n = 5) and presented as mean ± SEM.NS not significant, ND Not detectable.Parasitemia was analyzed using a Mann-Whitney U-test (only significance p < 0.05 is shown).Source data are provided as a Source Data file.

Fig. 6 |
Fig. 6 | Anti-Q586B2 Nbs exert a Q586B2-blocking potential on myeloid cells (BMDMs) in vitro as well as a therapeutic potential.a BMDMs generated from C57BL/6 mice (2 × 10 5 cells/well) were incubated with 1 µg/ml of LPS-free Q586B2 (white bars) or buffer alone (black bars in presence or absence of anti-Q586B2 Nbs (5 µg/ml)).After 48 h incubation the culture medium was tested for IL-10 production.Negative controls: cells incubated with buffer alone or without any protein.Dashed red line represent levels of cytokines produced by BMDMs stimulated with Q586B2 alone and all groups were compared to this signal.Data are shown as means ± SEM and representative of 2 independent experiments (n = 3).Analysis was performed using a one-way ANOVA with Brown-Forsythe and Welch's test (only significance p < 0.05 is shown).b, c Parasitemia development and survival of C57BL/6 mice infected with Wild-type T. b. brucei AnTat1.1Eparasites and treated with non-blocking anti-Q586B2 Nbs (blue box) or blocking anti-Q586B2 Nbs (orange box) starting from days 0 post-infection and this for 4 consecutive days (indicated by blue arrows).Results are presented as mean (parasitemia) or median (survival) of 5 individual mice ± SEM.Parasitemia was analyzed using a Mann-Whitney U-test (only significance p < 0.05 is shown) and survival curves were analyzed via a Mantel-Cox test (MS median survival).Source data are provided as a Source Data file.

Fig. 7 |
Fig. 7 | Q586B2 can be considered a broad-spectrum target for trypanosomes.a, b Parasitemia development and survival of mock-treated (black circles) and Q586B2-treated (white circles) C57BL/6 mice infected with Wild-type T. b. brucei (AnTat1.1E)parasites.c, d Parasitemia development and survival of mock-treated (black circles) and Q586B2-treated (red circles) C57BL/6 mice infected with T. congolense parasites.e, f Parasitemia development and survival of mock-treated (black circles) and Q586B2-treated (red circles) C57BL/6 mice infected with T. evansi parasites.g, h Parasitemia development and survival of mock-treated (black circles) and Q586B2-treated (red circles) C57BL/6 mice infected with T. cruzi parasites (RA strain).Results are presented as mean (parasitemia) or median (survival) of individual mice (n = 5) ± SEM.Parasitemia was analyzed using a Mann-Whitney U-test (only significance p < 0.05 is shown) and survival curves were analyzed via a Mantel-Cox test (MS median survival).Source data are provided as a Source Data file.