GAS2 encodes a 2-oxoglutarate dependent dioxygenase involved in ABA catabolism

Liu et al.1 recently reported the characterization of Arabidopsis thaliana GAS2 (Gain of Function in ABA-modulated Seed Germination 2), which was described as an enzyme that catalyzes the stereospecific hydration of GA12 to produce GA12 16, 17-dihydro-16α-ol (DHGA12). However, as previously reported2, we did not find conversion of [17-14C]-labeled or [1-,7-,12-,18-14C4]-labeled GA12 by GAS2. Furthermore, the authors1 state the isolation of endogenous DHGA12 from dry Arabidopsis seeds, which we cannot confirm by our attempts to isolate this compound from 0.5 g dry Arabidopsis Col-0 seeds (data not shown). Instead, we present here data showing that the recombinant GAS2 enzyme is able to catabolize abscisic acid (ABA) to phaseic acid (PA) and further to a second product, putative 8’-carboxy-ABA (Fig. 1a).

Liu et al. 1 recently reported the characterisation of Arabidopsis thaliana GAS2 (Gain of Function in ABA-modulated Seed Germination 2), which was described as an enzyme catalysing the stereospecific hydration of GA 12 to produce GA 12 16, 17-dihydro-16α-ol (DHGA 12 ).A second paper describes the conversion of GA 12 to an unidentified product by GAS2 and reports that this enzyme does not convert ABA 2 .As previously reported 3 , we did not find any conversion of [17- 14 C]labelled or [1-,7-,12-,18-14 C 4 ]-labelled GA 12 by GAS2.Instead, we present here data showing that the recombinant GAS2 enzyme catabolizes abscisic acid (ABA) to phaseic acid (PA) and further to a second product, putative 8'-carboxy-ABA (compound A; Fig. 1a).
ABA is known to be oxidatively catabolized by cytochrome P450 monooxygenases 4 .Three different hydroxylation pathways have been described that oxidise one of the methyl groups of the ring structure, at C-7', C-8', or C-9'.8'-hydroxylation appears to be the major pathway, and Arabidopsis CYP707As encode ABA 8'-hydroxylases that initiate the oxidation at C-8' to produce 8'-hydroxy-ABA, which spontaneously autoisomerises to PA 5,6 .This final cyclization step is reversible, and the final equilibrium is at PA 7 (Fig. 1a).
For this study, Arabidopsis GAS2 was heterologously expressed in E. coli according to our standard protocols 8,9 .Surprisingly, recombinant GAS2 does not convert 17,17-d2-GA 12 under either our incubation conditions or those described by Liu et al. 1 and by Xiong et al. 2 (Supplementary Fig. 1, right column).Note that Liu et al. show in Figure 3 1 that 17,17-d2-DHGA 12 accumulated after 5, 30, and 120 min, although they state in the same paper that "fresh cofactors were added after 2, 4, 6 and 8 h" and that the "reaction mixtures were incubated overnight at 30 °C".In contrast, recombinant GAS2 efficiently converts deuterated ABA regardless of the incubation conditions, as long as cofactors essential for the activity of 2-oxoglutarate-dependent dioxygenases are added (Supplementary Fig. 1, left column).As a control, Arabidopsis gibberellin 20-oxidase 1 (AtGA20ox1) was expressed in E. coli in the same way.No products are formed with AtGA20ox1 and deuterated ABA (Fig. 1b, top lane), indicating that the E. coli cell lysate is devoid of ABA-oxidising activities.Under our incubation conditions, lysate volumes of 1.6 µL or more are sufficient to completely convert the ABA substrate (Fig. 1b, left row).At low lysate volumes, an intermediate product is formed first, which disappears at high lysate volumes (PA, Fig. 1b, middle column) when a second product appears (compound A, Fig. 1b, right column).
Mass spectra and KRI of the intermediate product from incubations of GAS2 with ABA correspond to PA (Table 1).Interestingly, when 3′,5′,5′,7′,7′,7′-d6-ABA is used as substrate, d3-PA is formed while compound A retains its d6-labels.This indicates an exchange of deuterium for hydrogen, which preferentially occurs during the methylation of PA by diazomethane 10 .The two deuterium atoms at the 5′-carbon are the most easily exchanged.In addition, the deuterium atom at the 3′-carbon of PA is abstracted by diazomethane.When 7′,7′,7′-d3-labelled PA is the substrate it retains the d3-labels, indicating that the three deuterium atoms at the 7′-carbon are unlikely to be exchanged (Table 1).The different stability of the deuterium atoms in ABA and PA can be explained by the structural differences between the two compounds 10 and could indicate that compound A is structurally closer related to ABA than to PA.
There is no known ABA catabolite that matches the mass spectrum of compound A. In fact, compound A shares several fragment ions with ABA 11 , again suggesting structural similarities between the two compounds (Table 1).Unlabelled compound A shows a molecular ion (M + ) of 322 mass units in good agreement with ABA, containing an additional carboxyl group (as a methyl ester) most likely at the 8′position.This structure suggests that GAS2 catalyses the conversion of ABA and PA to 8′-carboxy-ABA via 8′-hydroxy-ABA, and 8′-aldehyde-ABA (the structure for the latter is not shown in Fig. 1a).Such intermediates have not been isolated, possibly because of their instability or rapid conversion 12 .However, such a stepwise oxidation pathway from methyl to the carboxyl group via the alcohol and aldehyde has been found in reactions catalysed by other 2-oxoglutarate-dependent dioxygenases, including gibberellin 20-oxidases 3 .In addition, compound A, together with ABA and PA, was identified as an endogenous compound in 31-day-old Arabidopsis shoots that had been droughtstressed for 17 days and then rehydrated for 3 days (Supplementary Table 1a).No endogenous DHGA 12 was detected in this material (Supplementary Table 1b).Liu et al. 1 identified endogenous DHGA 12 in imbibed Arabidopsis seeds, but our attempt to reproduce this finding was unsuccessful (Supplementary Fig. 2), suggesting that DHGA 12 may not consistently accumulate to appreciable levels (at least not in our conditions) arguing against a substantial biological role.
Xiong et al. 2 observed that Arabidopsis GAS2 overexpressing lines (here called GIM2) have decreased endogenous ABA levels, whereas the Arabidopsis knock-out mutant has increased endogenous ABA levels.Furthermore, GAS2 overexpressing lines show reduced sensitivity to ABA in germination and early seedling development compared to WT and increased sensitivity to ABA in GAS2 loss-of-function mutants 1 .In agreement with these observations and our own results, we conclude that the main function of the 2-oxoglutarate-dependent dioxygenase GAS2 is to initiate ABA catabolism, and that gibberellin anabolism, as proposed by Liu et al. 1 , maybe only a side reaction of this enzyme.

Heterologous expression of recombinant GAS2 and GA20ox1
AtGAS2 (AT2G36690) and AtGA20ox1 (AT4G25420) coding sequences were custom synthesised in the pET21a expression vector (BioCat), and sequence identities were verified by sequencing on both strands.The respective plasmid DNAs were used to transform BL21 Star TM E. coli (Invitrogen) according to the manufacturer's instructions.From the transformation reaction, 200 µL were used to inoculate 10 mL L-broth medium supplemented with carbenicillin (50 µg•mL −1 ), and transformed E. coli were grown for 16 h at 37 °C with shaking (250 rpm).From the previous E. coli culture, 2 mL were grown shaking (250 rpm) at 30 °C in 100 mL 2× YT broth with carbenicillin to OD 600 between 0.7 and 1.Expression was induced by the addition of IPTG to 1 mM; the culture was grown for a further 2 h at 30 °C and harvested by centrifugation (5,000 × g for 10 min at 4 °C).The pellet was resuspended in 200 µL of 200 mM Tris, pH 7.8 at 4 °C, containing 10 mM DTT and 1 mg .mL −1 lysozyme and the cells were disrupted by incubating on ice for 30 min, followed by four cycles of freezing in liquid N 2 and thawing.DNaseI (20U in 10 µL) were added to the E. coli cell lysate and incubated for 15 min at 20 °C.The lysates were centrifuged at 30,000 × g for 30 min at 4 °C to give approximately 500 µL of supernatant, which was used for enzyme assays 8,9 .Enzyme assays and purification of incubation products 3′,5′,5′,7′,7′,7′-d 6 -labelled ABA was purchased from OlChemIm, Czech Republic.PA and 7′,7′,7′-d3-PA were gifts from Professor Eiji Nambara (University of Toronto, Canada).Preparations of E. coli cell lysates were incubated in a total volume of 100 µL containing 100 mM Tris-HCl, pH 7.0 at 30 °C for 16 h with 2-oxoglutarate and ascorbate (100 mM each, final concentrations), FeSO 4 (0.5 mM), catalase (1 mg•mL −1 ), and the substrates (5 µL in methanol) ABA (500 ng), 3′,5′,5′,7′,7′,7′-d 6 -labelled ABA (500 ng), PA (500 ng), or 7′,7′,7′-d3-labelled PA (50 ng).Variations to the standard incubation conditions are indicated in the individual experiments.The enzymatic reactions were stopped by adding 10 µL acetic acid, which lowered the pH to about 3.2 and the incubation products were extracted two times with 500 µL methanol.

GC-MS analysis
The derivatized samples were analysed using a Thermo Scientific MS system ISQ 7000 with Advanced Electron Ionization (AEI) source equipped with a Thermo Scientific Trace 1300 gas chromatograph (GC-MS).The performance of the GC-MS was evaluated every day prior analyses by injecting authentic GA 4 as Me ester TMSi ether (0.1 ng in 1 µL in MSTFA).Samples (1-2 µL) were injected into an Agilent DB-5MS UI capillary column (30 m long, 0.25 mm i.d., 0.25 µm film thickness; Agilent, USA) at an oven temperature of 60 °C.The split value (30:1) was opened after 1 min, after which the temperature was increased by 45 °C min −1 to 175 °C and then with 4 °C min −1 to 280 °C.The He inlet was pneumatic pressure controlled at a constant flow rate of 1.2 mL .min −1 , and the injector, transfer line, and source temperatures were 280 °C, 280 °C and 250 °C, respectively.Mass spectra were acquired from 5 to 30 min after injection at an electron energy of 45 eV from 60 to 660 atomic mass units at 0.2 s per scan.For determination of Kovats retention index (KRI), 0.5 µL solution of 1 cm 2 parafilm in 2 mL hexane was injected.

Fig. 1 |
Fig. 1 | GAS2 is an ABA catabolising oxidase.a The proposed ABA catabolic pathway catalysed by GAS2.b Metabolism of 3',5',5',7',7',7'-d 6 -ABA incubated with 70 µL cell lysate containing recombinant ATGA20ox1 and cofactors (negative control, top lane).Metabolism of d 6 -ABA incubated with indicated volumes of cell lysates containing recombinant GAS2 and cofactors as described in Methods.Chromatograms of characteristic single ions are shown in the first row for ABA (extracted ion m/z 194.2), in the middle row for PA (m/z 125.2), and in the right row for compound A (m/z 194.2).Compounds that were identified on the basis of their full scan mass spectra of the methyl ester derivatives are shown in red.Similar results were obtained with two recombinant GAS2 preparations, produced from independent isolated E.coli clones, each were incubated at seven different lysate concentrations.

Table 1 |
Metabolism of d6-ABA, d3-PA, ABA, and PA by cell lysates from E. coli transformed with AtGAS2 in pET21a