Unexpectedly minor nitrous oxide emissions from fluvial networks draining permafrost catchments of the East Qinghai-Tibet Plateau

Streams and rivers emit substantial amounts of nitrous oxide (N2O) and are therefore an essential component of global nitrogen (N) cycle. Permafrost soils store a large reservoir of dormant N that, upon thawing, can enter fluvial networks and partly degrade to N2O, yet the role of waterborne release of N2O in permafrost regions is unclear. Here we report N2O concentrations and fluxes during different seasons between 2016 and 2018 in four watersheds on the East Qinghai-Tibet Plateau. Thawing permafrost soils are known to emit N2O at a high rate, but permafrost rivers draining the East Qinghai-Tibet Plateau behave as unexpectedly minor sources of atmospheric N2O. Such low N2O fluxes are associated with low riverine dissolved inorganic N (DIN) after terrestrial plant uptake, unfavorable conditions for N2O generation via denitrification, and low N2O yield due to a small ratio of nitrite reductase: nitrous oxide reductase in these rivers. We estimate fluvial N2O emissions of 0.432 − 0.463 Gg N2O-N yr−1 from permafrost landscapes on the entire Qinghai-Tibet Plateau, which is marginal (~0.15%) given their areal contribution to global streams and rivers (0.7%). However, we suggest that these permafrost-affected rivers can shift from minor sources to strong emitters in the warmer future, likely giving rise to the permafrost non-carbon feedback that intensifies warming. Permafrost soils can be substantial sources of nitrous oxide (N2O) to the atmosphere, but no data exist on the N2O footprints of permafrost rivers. Here, the authors show that alpine permafrost rivers are unexpectedly small sources of atmospheric N2O at present.

N itrous oxide (N 2 O) is a major stratospheric ozone destroyer and the third most important long-lived greenhouse gas (GHG) 1 . Sources of this powerful GHG are poorly constrained in general, and for global streams and rivers in particular 2 . Studies of lotic N 2 O dynamics have focused almost exclusively on human-impacted lowland systems across diverse climate zones, where anthropogenic nitrogen (N) enrichment has been linked to elevated N 2 O fluxes 3 . Unfortunately, data are extremely sparse for more pristine regions, including the rapidly changing permafrost-rich cryospheres at high altitudes and latitudes. As a result, the current estimate of global fluvial emissions of 291.3 ± 58.6 Gg N 2 O-N yr −1 has substantial uncertainty 4 .
Massive amounts of organic carbon (OC,~1014 Pg C) are stored in the top 3 m of Northern Hemisphere permafrost soils 5 . As these deposits thaw, ice-locked carbon is liberated and can be transported to adjacent running waters, where it becomes available for processing and loss to the atmosphere as carbon dioxide (CO 2 ) and methane (CH 4 ) [6][7][8][9] . Consequently, fluvial emissions of these gases in some cryosphere regions are now occurring at elevated and increasing rates 10,11 . However, the magnitude of N 2 O emissions from streams and rivers in regions where permafrost thaw is afoot is unknown despite the fact that Northern Hemisphere permafrost soils contain 67 Pg N to a depth of 3 m (excluding N pools in the active layer), and are evident or even substantial sources of N 2 O 12 . Studies of Alaskan streams have emphasized sustained delivery of N from thawing permafrost soils resulting in elevated inorganic N 13,14 and oftensupersaturated N 2 O concentrations in these receiving waters 13 . However, in the absence of direct measurements of N 2 O emissions from permafrost-affected streams and rivers, it is not clear if these results are representative and translate to overall elevated N 2 O emissions.
To address this knowledge gap, we provide a cross-regional and seasonal direct measurement of fluvial N 2 O concentrations and fluxes, generated in four headwater catchments that vary in altitude from 1650 to 4600 m and cover an area of ca. 73.6 × 10 4 km 2 on the East Qinghai-Tibet Plateau (EQTP; Fig. 1; Supplementary Fig. 1, Table 1 and 2), and identify likely mechanisms shaping N 2 O dynamics in these rivers. Areal CH 4 emissions from streams and rivers in this region are strongly affected by permafrost thaw, and are among the highest reported rates globally 11 , suggesting that a similar effect may be underway for N 2 O in these rivers as well. This region is the largest cryosphere outside the Arctic and Antarctic 15 , with vast Pleistoceneaged permafrost 16 that contains an estimated 1.8 Pg N in the upper 3 m of soil 17 . As the 'Water Tower of Asia', meltwater from the Qinghai-Tibet Plateau (QTP) feeds ten great Asian rivers and ubiquitous ponds, lakes, and wetlands 18 . Such alpine streams and rivers are tightly connected to permafrost soils 11 and have high turbulence-induced gas exchange 19 , and these features potentially promote their capacity to process terrestrial N and sustain high N 2 O emissions. Further, many parts of the QTP have experienced significant human population growth over the last six decades accompanied by similar rapid increases in livestock 20 , again adding to the potential for enhanced riverine N 2 O emissions. However, as we show below, these alpine permafrost-affected streams and rivers of the EQTP are unexpectedly minor atmospheric N 2 O sources. Yet there are signs that developing climate warming and enhanced anthropogenic influences may lead to a substantial increase in fluvial N 2 O emissions from high altitudes and latitudes in the coming decades. This study offers a guide to include permafrost streams and rivers in current and future N 2 O inventories, given their potentially important implications for N 2 O budget.

Results and discussion
Variability of N 2 O concentrations and fluxes. All sampled streams and rivers were supersaturated on all dates (117.9-242.5%, n = 342 samples from 114 site visits) in N 2 O with respect to the atmosphere. Dissolved N 2 O concentrations fluctuated between 10.2 and 18.9 nmol L −1 with an average of 12.4 ± 1.7 nmol L −1 , which is one-third of the global average 3 Fig. 1 Map of the study sites in four headwater catchments on the East Qinghai-Tibet Plateau (EQTP), China. Sampling sites within the four catchments. The blue shading represents permafrost extent on the EQTP (Data for the permafrost extent courtesy of ref. 57 ). The inset highlights the study area on a map of China.  Table 3). Significantly higher N 2 O concentrations were observed in spring (P < 0.001), followed by fall and summer ( Supplementary Fig. 2a). Despite differences in catchment attributes including permafrost fraction and population densities (Supplementary Table 2), N 2 O concentrations were not significantly different among the four river systems ( Supplementary Fig. 2a).
Diffusive N 2 O fluxes from EQTP rivers were predominantly positive (to the atmosphere), ranging from −14.0 to 40.6 µmol m −2 d −1 with an average of 9.4 ± 6.2 µmol m −2 d −1 (n = 436 samples from 114 site visits). This mean flux is an order of magnitude lower than the global average 3 (94.3 µmol m −2 d −1 ; Supplementary Table 3). Diffusive N 2 O fluxes were similar in summer and fall, and significantly higher than those in spring (P < 0.05; Supplementary Fig. 2b). The asynchronous seasonal patterns between concentrations and fluxes are likely caused by water temperature and precipitation (Supplementary Discussion 1). As with concentration, no significant differences were found for N 2 O diffusion among the four rivers ( Supplementary Fig. 2b). N 2 O ebullition has seldom been documented in lotic ecosystems, although it can coincide with CH 4 bubble release 21 . Because of the presence of large organic reserves in surrounding permafrost, shallow water depths, and their exposure to low barometric pressure, streams and rivers on the EQTP are notable hotspots of CH 4 ebullition 11 . Thus, we had hypothesized that N 2 O would also be entrained during the widespread processes of CH 4 bubble release. The mean N 2 O ebullition rate from EQTP rivers was 0.74 ± 2.47 µmol m −2 d −1 , and accounted for 4.1 ± 11.9% of total N 2 O fluxes (diffusion + ebullition) across all sites. Despite this potential for high ebullition rates, accounting for this flux had a little overall effect, as the average total N 2 O flux (10.2 ± 7.1 µmol m −2 d −1 ) was still nine-fold lower than the global mean diffusive N 2 O.
Terrestrial processes modulating N 2 O dynamics. The availability of inorganic N is often the primary determinant of rates of N 2 O production and emission in both permafrost-affected soils 12 and fluvial networks 2 . In the QTP, dissolved N released from both thawing permafrost soils and animal manure (Supplementary Discussion 2) is biologically available for plant uptake [22][23][24] or instead may be exported to river channels. Because plant growth is N-limited in this region 24 , we predicted that an increase in vegetation cover would result in greater plant uptake of terrestrial N, and consequently lower inputs to, and concentrations of N in streams and rivers, up to a point. Indeed, riverine dissolved inorganic N (DIN) concentrations decreased with increasing vegetation cover for all sites (Fig. 2a). However, mechanisms causing the observed decline in riverine DIN cannot be elucidated from vegetation coverage alone, as productivity and greenness per unit plant cover decline at higher elevations 25 . Thus, we also examined normalized difference vegetation index (NDVI, a measure of productivity and greenness) to indicate plant N uptake. Sites with high NDVI had low riverine DIN in noncontinuous (namely discontinuous, sporadic, and isolated) permafrost zones (Fig. 2b), in line with the hypothesis of greater plant influence on riverine N availability when and where vegetation productivity and greenness were higher. In contrast, in areas with continuous permafrost, sites with high NDVI had high riverine DIN (Fig. 2b), suggesting that terrestrial N was sufficient to support plant growth and associated productivity/greening regardless of season, and indeed likely exceeded the N demand of plant community 26 . If so, then the surplus DIN can be exported to river corridors. Terrestrial N 2 O can also be transported along with DIN to surrounding watercourses, and higher N 2 O concentrations in some sites draining permafrost areas could be supported by transport of terrestrial N 2 O and/or greater in situ N 2 O production supported by more DIN inputs. Even so, EQTP streams and rivers collectively received reduced terrestrial N after plant uptake. DIN concentrations (0.54 ± 0.30 mgN L −1 ) in EQTP waterways were at the lower end of the range reported for global streams and rivers 3 (0.002-21.2 mgN L −1 ) and constrained within a relatively narrow range. The low N 2 O concentrations and fluxes are consistent with the low N availability in these rivers.
Biogeochemical processes regulating N 2 O dynamics. Riverine N 2 O concentrations could not be effectively predicted by simple linear regressions with environmental variables [R 2 ≤ 0.1 in most cases, including dissolved oxygen (DO, P > 0.05, R 2 = 0.004) and NH 4 + concentrations (P < 0.001, R 2 = 0.1); Supplementary Table 4]. However, we found a strong positive relationship between NO 3 − and N 2 O concentrations when DO saturation (% O 2 ) was undersaturated (<100%) in the water column (Fig. 3a). This result was validated by a regression tree analysis that   27 , and in EQTP rivers, the N 2 O yield (0.003-0.87%, average 0.23%) was 11 times lower than has been reported for lotic settings (0.01-53.8%, average 2.47%; Supplementary Table 5). This small percentage indicates that N processing in EQTP streams and rivers predominantly generates dinitrogen (N 2 ) instead of N 2 O. Laboratory determination of benthic N 2 O production rates confirmed the consistent conversion of N 2 O to N 2 , as these rates were negative (N 2 O was consumed) for twothirds of the sites (Supplementary Table 6). Low N 2 O yields have been associated with the availability of ample OC to support the complete reduction of NO 3 − to N 2 2 , and in addition to supplying N, thawing permafrost is also a source of biolabile OC to EQTP streams and rivers 11 (Fig. 4a).
Examination of relative gene abundances involved in N 2 O production and consumption provides further insights regarding the reason for the low N 2 O yield in EQTP rivers. The key enzymes for N 2 O production are two types of nitrite reductase (nirS and nirK) 31 , and N 2 O consumption is mediated by clade I and II nitrous oxide reductase (nosZ) which catalyzes N 2 O reduction to N 2 32 . A high ratio of (nirS + nirK)/nosZ indicates an amplified capacity for N 2 O production relative to its loss, leading   was highest in 3 rd -order (headwater) streams, declined in 4 th -and 5 th -order (medium-sized) rivers, and was slightly elevated in 6 th -and 7 th -order (large) rivers (Fig. 3c). The decline in flux from 3 rd -to 5 th -order streams may reflect the reduced perimeter-to-surface-area ratio (ratio of wetted perimeter to cross-sectional area) and hyporheic exchange rates (exchange rates of dissolved substances between surface water and groundwater beneath and alongside the river channel) with increasing stream order [34][35][36] , while the increase in 6 th -and 7 th -order sites might be due to increasing riverine DIN concentrations 2 ( Supplementary Fig. 4). Furthermore, increased suspended sediment loads can enhance N 2 O generation in larger turbid channels, as suspended particles provide micro-niches that support N transformations 37 , and thus facilitate N 2 O production in the water column 2,36 . Suspended sediment concentration increased with stream order for these rivers 11 , lending support to the hypothesis that this mechanism contributes to higher fluxes observed in 6 th -and 7 th -order channels.
Regional and global implications. Based on our flux measurements, we estimated that EQTP 3 rd -to 7 th -order streams and rivers emitted 0.206 Gg N 2 O-N yr −1 (5-95 th percentiles: 0.129-0.291 Gg N 2 O-N yr −1 , 2603 km 2 of river channel area). Our upscaling did not include 1 st -and 2 nd -order streams, which can contribute disproportionately high areal fluxes to overall fluvial N 2 O emissions 36,38 . Low-order streams are always well connected to continuous permafrost and hence should receive high N inputs while having reduced N 2 O solubility owing to high altitudes, and these conditions are expected to lead to high N 2 O fluxes. Based on this logic, we estimated a total emission of 0.275 Gg N 2 O-N yr −1 from 1 st -to 7 th -order streams and rivers (5-95 th percentiles: 0.162-0.400 Gg N 2 O-N yr −1 , 3049 km 2 ) by extrapolating the relationship in Fig. 3c to include 1 st -and 2 nd -order streams.
Despite large uncertainties due to a lack of observational data from 1 st -and 2 nd -order streams (Supplementary Discussion 4), the upscaling exercise enables us to place our estimates in a broader context of both regional N 2 O budgets and fluvial emissions at the global scale. The percentage of N 2 O in total GHG (CO 2 + CH 4 + N 2 O) emissions expressed as CO 2 equivalents corresponded to 1.0% for EQTP 3 rd -to 7 th -order drainages, then dwindled to 0.4% for EQTP 1 st -to 7 th -order streams and rivers, falling within the range of pristine rivers (0.2-1.2%) 39,40 . These values contrast those from human-impacted fluvial networks, where N 2 O percentages are generally much higher (2.8-13.9%, average 6.8%; Supplementary Table 9) due to often elevated inputs of fertilizer-or sewage-derived N that boost N 2 O emissions. Expressed as per unit stream/river and basin area, EQTP 1 st -to 7 th -order streams and rivers released a total of 0.08 t N 2 O-N km −2 yr −1 and 0.32 kg N 2 O-N km −2 yr −1 , respectively, to the atmosphere, which are one order of magnitude lower than those from lotic systems worldwide [0.65 (range: 0.08-2.55) t N 2 O-N km −2 yr −1 and 2.44 (range: 0.55-5.78) kg N 2 O-N km −2 yr −1 , respectively; Supplementary Table 9]. Applying the emission rate per unit stream/river and basin area to the entire QTP 1 st -to 7 th -order drainage networks, we obtained a riverine N 2 O emission of 0.432-0.463 Gg N 2 O-N yr −1 , which is minor (~0.15%) given their areal contribution (0.7%) to global streams and rivers 41 . In addition, these N 2 O estimates are probably overestimated. The emission of N 2 O accumulated underneath the ice during winter was estimated to be 15% of the annual emission 39 . This ice-melt outgassing of winter N 2 O was included in the above annual N 2 O emissions; however, this flux may be very limited in permafrost-affected systems due to minimum N inputs from frozen soils in winter 42 . These alpine permafrost waterways emit large amounts of CH 4 11 , but fortunately they are currently small contributors of N 2 O delivery to the atmosphere, demonstrating CH 4 and N 2 O dynamics are uncoupled within these systems.
Although QTP fluxes were small, existing global estimates do not effectively capture this natural fluvial source of N 2 O, nor are the major drivers of N 2 O dynamics well known for these systems. Our study is a step forward in quantifying fluvial N 2 O evasion from a cryospheric biome, and highlights the unique dynamic nature of N 2 O concentrations and fluxes in high-altitude environments. Our finding that oxygen saturation was the first and primary correlate of N 2 O concentrations may also have broader implications for aquatic N 2 O dynamics in other high-altitudinal streams.
Future fluvial N 2 O emissions under warming climate. Temperatures are rising faster in high altitudes and latitudes than in other regions 43 . As warming continues, permafrost thaw is expected to increase, liberating substantial amounts of dissolved N 12 . As this process progresses into deeper soil layers below the rhizosphere, diminished plant uptake 24 should favor greater export of N to streamflow via deep flow paths 14,44,45 . Meanwhile, warmer water temperatures reduce gas solubility and enhance hypoxia and denitrification at the expense of anammox 46 , directing more N towards denitrification, and concomitant N 2 O production and evasion to the atmosphere (Fig. 5). Moreover, the duration of the ice-free season across the cryosphere is rapidly increasing and will continue to increase 47 , suggesting a potential proxy for riverine N 2 O release. Furthermore, human perturbations may bring an extra N burden to the cryosphere and exacerbate these impacts. Taken together, these processes might render streams and rivers draining permafrost catchments across the globe to become hotspots of N 2 O to the atmosphere in the future, leading to positive non-carbon climate feedback of currently unanticipated magnitude because of an increase in fluvial N 2 O production following the development of climate change and escalation of anthropogenic influence.
The high degree of spatiotemporal variability in riverine N 2 O observed here is likely to exist in other unexplored cryospheres. Further progress in understanding how aquatic ecosystems in these climate-sensitive regions will respond to ongoing global warming would benefit greatly from more N 2 O measurements from glacial and permafrost-affected lotic and lentic systems at high spatial and temporal resolution. Alongside N 2 O measurements, capturing the fate of thawed N in cryospheric aquatic systems are indispensable to stitching together pathways and processes into a holistic framework. It is time to improve our understanding of cryospheric aquatic N 2 O emissions in shaping the global N 2 O budget, and how this contribution might be altered in progressively warming high altitudes and latitudes.

Methods
Site description. The~73.6 × 10 4 km 2 study area of the East Qinghai-Tibet Plateau (EQTP) is located between the boundary of the Loess Plateau (Gansu Province, 36°N ) and the southern frigid limit (Yunnan Province, 28°N). This region is characterized by a high-relief landscape ranging from 1650 to 7000 m above sea level. The elevational gradient, along with abundant but variably distributed permafrost creates geomorphological, hydrological, vegetative, and climatological heterogeneities in the alpine landscape. The EQTP includes four great Asian rivers-the Yangtze, Yellow, Lancang-Mekong and Nu-Salween Rivers. We sampled a broad range of streams and rivers in these basins that ranged from Strahler order 3 to 7. All sampling sites were visited during the daytime in spring (May-June), summer (July-August), and fall (September-October) between 2016 and 2018. The Yellow River was sampled seven times, the Yangtze River four times, and the Lancang and Nu Rivers were both sampled on three dates. We grouped the sampling sites into four categories that represent different permafrost zones: continuous, discontinuous, sporadic, and isolated (Fig. 1). We then merged discontinuous, sporadic, and isolated permafrost zones together under the non-continuous permafrost group in response to different NDVI trends. N 2 O concentrations and fluxes. Triplicate samples for dissolved N 2 O concentrations were collected by completely filling 120 mL glass serum bottles at wrist depth below the water surface at each site. After preserving samples with 0.5 mL saturated ZnCl 2 solution, the serum bottles were sealed with butyl stoppers, crimped with aluminum caps, and stored at ambient temperature in the dark. Local ambient air samples were also taken and used to back-calculate N 2 O concentration in water in equilibrium with the atmosphere. N 2 O concentrations were determined via the headspace equilibration method 48 on a gas chromatography equipped with an electron capture detection for N 2 O (Agilent 7890B GC-µECD). The partial pressure of CO 2 (pCO 2 ) in surface water was determined following our earlier work 11 . Sampling and analysis of dissolved N 2 concentrations are described in Supplementary Methods 1.
F N 2 O was measured simultaneously with dissolved N 2 O concentration collection. Four floating chambers were held in place at each transect, covering depth gradients from the river bank to the mid-channel to capture the spatial heterogeneity. Measurements lasted for 1 h at each site, and 50 mL gas extracted from inside the chambers at 0, 5, 10, 20, 40, and 60 min intervals were injected into air-tight gas sampling bags for analysis in the laboratory by GC-µECD. These chambers were of the same size and shape and streamlined with a flexible plastic foil collar to minimize the effects of chamber-induced turbulence when measuring fluxes 49 and were covered with aluminum foil to reflect the sunlight and minimize internal heating.
Surface water and sediment samples were collected simultaneously with gas samples for physicochemical and microbial analyses at each site, respectively. Air temperature, air pressure, and wind speed were measured in situ with a portable anemometer (Testo 480). DO, pH, ORP, conductivity, and water temperature were measured in situ with portable field probes (Hach HQ40d). Annual air temperature and precipitation were obtained from the National Meteorological Information Center (http://data.cma.cn/). Flux computation. Total F N 2 O were calculated according to the equation below: where, n t and n 0 are the number of moles of N 2 O in the chamber at time t and time zero (mol), respectively; A is the surface area of water covered by the chamber (m 2 ) and t is the measurement duration time (min). Diffusive and ebullitive N 2 O fluxes were separated using the Campeau et al. approach 50 . Briefly, we assumed that F t for CO 2 (F CO2 ) is exclusively diffusive (that is, CO 2 ebullition is negligible). F CO2 was computed from the linear regression of pCO 2 against time to eliminate possible bias due to gas accumulation in the chamber headspace that can affect the flux rates. We then used F CO2 to calculate k CO2 by rearranging the equation for Fick's law of gas diffusion 51 : where K H is the temperature-adjusted Henry's constant, and ΔpCO 2 is the CO 2 partial pressure difference between surface water and the atmosphere. Finally, the theoretical diffusive k for N 2 O was calculated based upon k CO2 as follows 52 : where k N2O and k CO2 are gas transfer velocity of N 2 O and CO 2 , respectively; Sc is the Schmidt number. n = 1/2 is used for rippled or turbulent surface water conditions, or n = 2/3 is used for a smooth water surface 53 . We then calculated the theoretical diffusive F N 2 O according to where k is gas transfer velocity (m·d −1 ), C water is water gas concentration (mol m −3 ), and C eq is gas concentration in water in equilibrium with the local atmosphere corrected for temperature-induced changes in solubility according to the Henry's law (mol m −3 ). Thus, the difference between the total and diffusive N 2 O fluxes is attributable to ebullition.
Physicochemical analyses. Duplicate filtered water samples were analyzed for NH 4 + -N, NO 2 − -N, and NO 3 − -N, as well as dissolved organic carbon (DOC) following detailed methods described in ref. 11 . Total phosphorus (TP) was measured on a UV − Vis spectrophotometer (Agilent 1200) using the standard molybdenum blue method after persulfate digestion. Determination of sedimentary N 2 O production rates is described in Supplementary Methods 2. from approximately 0.5 g fresh homogenized sediment using the FastDNA ® SPIN Kit for Soil (MP Biomedicals), following the manufacturer's instructions. Abundances of dissimilatory nitrite reductase (nirS and nirK) 31 and nitrous oxide reductase (nosZ I and nosZ II ) genes 32 in these river sediments were estimated by real-time quantitative PCR. Detailed information is available in Supplementary Methods 3.
GIS analyses. Vegetation types in the study area are alpine meadow and steppe, and vegetation coverage for each type was extracted using datasets from ref. 54 . The vegetation coverage for each river reach was determined within a circular area of 5 km in radius centered around each sampling site using the ArcGIS 10.6 buffer analysis tool. Normalized difference vegetation index (NDVI) data were obtained from the China Monthly Vegetation Index Spatial Distribution Data Set (Resource and Environment Science and Data Center, Chinese Academy of Sciences, https:// www.resdc.cn/), with a spatial resolution of 1 × 1 km 2 for the period 2016-2018 corresponding to sampling dates. The NDVI values for each river reach were calculated as the mean value within a circular area of 5 km in radius centered around each sampling site using the ArcGIS 10.6 zonal statistics tool. Hydrological analysis was performed on the ArcGIS 10.4 platform. Streams and rivers were extracted and Strahler stream order for all stream lines in the four catchments was calculated based on Digital Elevation Model (DEM) data. We then calculated the total surface area for each stream order by multiplying the total length with the average width of this stream order. The total length was derived from the distance tool in the ArcGIS. We calculated the average river width for each stream order based on in situ width measurements at the sampling location and 50 additional locations of the corresponding stream order from Google Earth Maps in each catchment. Average stream width for the 1 st -and 2 nd -order streams were obtained from the width ratios found for rivers of high stream orders according to our earlier work 11 .
Upscaling technique. We upscaled the magnitude of N 2 O emissions (and uncertainty) from EQTP streams and rivers with a Monte Carlo simulation (MATLAB R2018b) that ran 1000 iterations for each sampled 3 rd -to 7 th -stream order. Each iteration randomly selected a N 2 O flux measurement and simultaneously selected a surface area based on a normal distribution surrounding the mean and standard deviation for that stream in order to generate an order-specific N 2 O flux per unit of time. This value was then summed across the ice-free season (210 d from April to October) to estimate N 2 O emission for each stream order. For 1 st -and 2 nd -order streams, a range of N 2 O fluxes that extrapolated from the relationship in Fig. 3c and the surface area for this order were used to produce total fluxes and constrain the uncertainty. We summed up the final distribution of N 2 O emissions from stream orders 1-7, including the mean value and 95% confidence intervals. Finally, we divided the ice-free season emission by 85% to obtain the annual emission. This is because early spring efflux of winter-derived N 2 O fuels 15% of the annual emissions 39 .
Statistical analyses. Pearson correlations and multiple linear stepwise regression analysis were conducted under the Statistical Product and Service Solutions 25.0 software (SPSS) at a significance level of P < 0.05. To uncover complex dependencies among predictor variables, we used a regression tree approach to analyze predictors of N 2 O concentrations with MATLAB R2018b. This nonparametric method does not require linear relationships and allows for interaction effects among predictors. All data form a single group at the top of the tree, and the tree is grown by repeatedly splitting the data into two subgroups, with each split based on the explanatory variable which makes the resulting groups as different as possible. All values of predictive variables are assessed each time a dichotomy is made 55 . For each of the two subgroups, the same process was repeated until a tree is formed. Such a tree needed to be pruned to avoid overfitting and improve predictive accuracy. We then applied 10-fold cross-validation and pruned the tree based on the '1-SE' rule. This was a parsimonious approach to find the smallest tree whose cross-validated relative error (CVRE) is within one standard error of the minimum. We report the amount of variance explained as R 2 to depict the fit of the tree. We also report the CVRE since it is a suitable measure of prediction 56 . Predictive variables available for entry into the model were: %O 2 and NO 3 -. Given the high CVRE here, the model is more suggestive than predictive.
Reporting summary. Further information on research design is available in the Nature Research Reporting Summary linked to this article.