Fig. 6: Cytosolic DNA exposure in high-risk UM. | Nature Communications

Fig. 6: Cytosolic DNA exposure in high-risk UM.

From: Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression

Fig. 6

a Baseline micronuclei frequency across UM cell lines, arranged from left to right based on their GEP2 score. Bar represents median; points, measured frequency of micronuclei per high-power field evaluated across three experimental replicates. Statistical significance tested using two-sided student t test; p = 2.8 × 10−8. Source data are provided as a Source Data file. b Micronuclei frequency in low-risk UM cells (92.1) upon PRT treatment as a function of time. For (a, b), bar represents median; points, measured frequency of micronuclei per high-power field evaluated across three experimental replicates. Statistical significance tested using two-sided student t test; p = 7.7 × 10−8. Source data are provided as a Source Data file. c Low-risk UM cells (92.1) stained for DAPI (white); showing increased nuclear size and micronuclei formation upon PRT-treatment. Images representative of experimental triplicates. d Micronuclei frequency in UM cells (Mel285.Cas9) upon RNF2 knockout. Bar represents median; points, measured frequency of micronuclei per high-power field evaluated across three experimental replicates. Statistical significance tested using two-sided student t test; p = 7.7 × 10−6. Source data are provided as a Source Data file. e An example of a cGAS (green) localization to micronuclei (blue). Representative image from biological triplicates.

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