Fig. 5: Increased chromosome segregation errors upon PRC1 inhibition. | Nature Communications

Fig. 5: Increased chromosome segregation errors upon PRC1 inhibition.

From: Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression

Fig. 5

a Volcano plots showing the top differentially expressed pathways in low-risk UM cells (92.1) treated with PRT vs DMSO for 24 h; evaluated across biological triplicates. Normalized enrichment score (NES) for selected genesets shown as a function of significance, -log10(FDRq); FDRq, Bonferroni corrected p value. An unfiltered list of all significant gene signatures is provided in Supplementary Data File 5. b Examples of UM cells in anaphase stained for DAPI (blue) and centromeres (red); demonstrating different patterns of chromosome segregation errors. Additional patterns of missegregation are shown in Supplementary Fig. 9b. Representative images from biological triplicates. c Abundance of chromosome segregation error patterns during anaphase in low-risk UM cells (92.1) upon PRT-treatment as a function of time. Statistical significance tested using two-sided unpaired Student’s t test. Source data are provided as a Source Data file. d Abundance of chromosome segregation error patterns during anaphase across UM cell lines, arranged from left to right based on their GEP2 score. Source data are provided as a Source Data file. e Western blot of H2AK119Ub, STING, RING1, and RNF2 relative to Actin in Mel285 cells upon RING1 and RNF2 knockout. Data representative of biological triplicates. f Abundance of chromosome segregation error patterns during anaphase in UM cells (Mel285.Cas9) upon RNF2 knockout. Statistical significance tested using two-sided unpaired Student’s t test. Source data are provided as a Source Data file. Stacked bars, mean of each missegregation pattern; error bars, standard error of the mean across experimental triplicates (c–f).

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