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Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle

Subjects

Abstract

The planktonic synthesis of reduced organophosphorus molecules, such as alkylphosphonates and aminophosphonates, represents one half of a vast global oceanic phosphorus redox cycle. Whilst alkylphosphonates tend to accumulate in recalcitrant dissolved organic matter, aminophosphonates do not. Here, we identify three bacterial 2-aminoethylphosphonate (2AEP) transporters, named AepXVW, AepP and AepSTU, whose synthesis is independent of phosphate concentrations (phosphate-insensitive). AepXVW is found in diverse marine heterotrophs and is ubiquitously distributed in mesopelagic and epipelagic waters. Unlike the archetypal phosphonate binding protein, PhnD, AepX has high affinity and high specificity for 2AEP (Stappia stellulata AepX Kd 23 ± 4 nM; methylphosphonate Kd 3.4 ± 0.3 mM). In the global ocean, aepX is heavily transcribed (~100-fold>phnD) independently of phosphate and nitrogen concentrations. Collectively, our data identifies a mechanism responsible for a major oxidation process in the marine phosphorus redox cycle and suggests 2AEP may be an important source of regenerated phosphate and ammonium, which are required for oceanic primary production.

Introduction

Phosphonates are reduced organic phosphorus (P) molecules with a carbon (C)–P bond, as opposed to the more common C-oxygen (O)–P ester bonds found in many other organic P molecules1. Phosphonates are synthesised as both primary and secondary metabolites in various bacterial, archaeal and eukaryotic organisms1,2,3,4,5,6,7 where they are incorporated into lipids (phosphonolipids) and glycans (phosphonoglycans)4,8. A significant proportion can also be released from the cell to facilitate favourable biotic interactions9. Thus, they are ubiquitous in terrestrial and aquatic ecosystems10,11,12,13,14. Phosphonates also represent a major fraction of the marine organic P pool11,15,16,17 and recent studies have now identified several cosmopolitan marine microorganisms capable of synthesising significant quantities of these compounds6,7,9,18,19. Collectively, this synthesis drives a vast global oceanic P redox cycle with reduced P input in the surface ocean estimated to be an order of magnitude greater than (non-anthropogenic) riverine P input9. Whilst much attention has focused on the degradation of alkylphosphonates, such as (hydroxy-)methylphosphonate and 2-hydroxyethylphosphonate, as a source of P16,17,20,21, the potential for aminophosphonates such as 2-aminoethylphosphonate (2AEP) to serve as sources of C and/or nitrogen (N) in the presence of inorganic phosphate (Pi), i.e. in a Pi-insensitive manner, has been neglected. However, emerging evidence suggests that Pi-insensitive 2AEP catabolism occurs in nature22,23. Notably, the fact that 2AEP is either absent from, or a minor component of, otherwise phosphonate rich high molecular weight dissolved organic matter (HMW DOM)16,17, despite its supposed ubiquitous production9,19,24,25, suggests preferential catabolism of this molecule in comparison to alkylphosphonates.

Unlike the majority of C–O–P monoester bonds, the C–P bond requires specific enzymes to break it, such as the C-P lyase26,27. Several 2AEP-specific phosphonate degradation systems have been characterised (Fig. 1a), including the 2AEP transaminase (PhnW) – phosphonoacetaldehyde hydrolase (phosphonatase-PhnX) system28,29 and the PhnW – phosphonoacetaldehyde dehydrogenase (PhnY)-phosphonoacetate hydrolase (PhnA) system30,31,32. The phosphonate dioxygenase (PhnY*) - phosphohydrolase (PhnZ) system has also been shown to degrade 2AEP33,34, though at least some homologs of this system are specific to (hydroxy-)methylphosphonate and cannot degrade 2AEP35,36. In addition, a gene encoding a recently characterised (R)-1-hydroxy-2-aminoethylphosphonate ammonia lyase (PbfA) is often found in phnWX and phnWAY operons37, expanding the known repertoire of aminophosphonate degrading capabilites37. The C-P lyase, which is a non-specific promiscuous phosphonate degrading enzyme complex, is only induced in response to Pi-starvation, being regulated by the two-component master regulator of the Pi-stress response regulon, PhoBR24. In marine surface waters, genes encoding the C-P lyase are enriched in bacterial genomes found in regions typified by low Pi concentrations20 where they are also heavily expressed38. Recent data has shown Pi-insensitive regulation of 2AEP degradation, facilitated by the 2AEP-specific systems, occurs in a few strains of bacteria related to marine Alphaproteobacteria22 and a terrestrial gammaproteobacterium23. In both cases, a major consequence of Pi-insensitive 2AEP degradation was the remineralisation and release of labile Pi22, due to the greater cellular demand for N over P and the 1:1 N:P stoichiometry of 2AEP.

Fig. 1: 2AEP transport and catabolism in P. putida BIRD-1.
figure1

a Schematic representation of candidate (highlighted in bold and coloured) routes for 2AEP transport, together with existing characterised and putative 2AEP transport routes. Each catabolic system for degradation is highlighted and includes (i) the phosphonatase system comprising a 2AEP-pyruvate transaminase (PhnW) and either a phosphonoacetaldehyde hydrolase (PhnX)28,29 or a NAD+-dependent phosphonoacetaldehyde dehydrogenase (PhnY)32 and a phosphonoacetate hydrolase (PhnA)30,31, and (ii) the PhnY*Z system comprising phosphohydrolase (PhnZ)33,34 and a 2-oxoglutarate dioxygenase (PhnY*)33. In addition, the promiscuous multi-subunit enzyme C-P lyase (PhnGHIJKLMN) can also act on 2AEP26,27, as well as akylphosphonates. Pathways found in BIRD-1 are represented by black lines; pathways absent from BIRD-1 are shaded grey. Characterised pathways are shown with solid lines; uncharacterised pathways are shown with dashed lines. Transporters found in BIRD-1 are red if Pi-sensitive, green if Pi-insensitive, and yellow if constitutive. Unknown mechanisms are denoted by a ‘?’. b Phylogenetic tree of AepX, PhnS, and AepS, using the characterised Fe3+ substrate-binding protein FbpA from Haemophilus influenzae and Bordetella pertussis as an outgroup. P. putida = Pseudomonas putida BIRD-1, S. stellulata = Stappia stellulata, B. cepacia = Burkholderia cepacia, B. vireti = Bacillus, S. merionis = Streptococcus merionis, S. typhimurium = Salmonella typhimurium, S. diastatochromogenes = Streptomyces diastatochromogenes, B. thuringiensis = Bacillus thuringiensis. c Growth (n = 4) of P. putida BIRD-1 wild type, ΔaepXVW, and the complemented mutant. d, Growth (n = 4) of P. putida BIRD-1 wild type, ΔaepP and the complemented mutant, and (e), Growth (n = 4) of P. putida BIRD-1 wild type, the double mutant, ΔaepXVW:ΔaepP, and the 2AEP null transporter mutant, ΔaepXVWaepP:ΔaepSTU. All strains used 2AEP as a sole P (top panel) or N (bottom panel) source. Error bars denote standard deviation of the mean.

When analytical methods are not sensitive enough to accurately quantify the concentration and turnover of specific environmental metabolites, screening for the expression of their respective uptake systems becomes an important tool in understanding their in situ cycling38,39,40,41. To date, only two 2-AEP transport systems have been identified. Both are ATP-binding cassette (ABC) transporters that consist of a periplasmic substrate-binding protein, an ATP-binding domain protein, and a transmembrane permease. The first is located within the C-P lyase operon (phnCDEFGHIJKLMNOP)42,43 with genes encoding the substrate-binding protein, ATP-binding domain and transmembrane domains designated phnD, phnC, and phnE, respectively. The second is another ABC-transporter PhnSTUV shown by Jiang et al. to complement a C-P lyase knockout mutant of E. coli together with phnWX28,29. PhnD has a restricted distribution in seawater with an abundance that is highly correlated with regions of Pi-limitation20. However, no 2AEP transporter has been identified in the majority of bacteria possessing PhnWX and PhnWAY systems, which is surprising given the fact that 2AEP is a charged molecule and ubiquitous in marine and terrestrial ecosystems1,20.

Here, we sought to identify transporters required for 2AEP catabolism in environmental bacteria lacking PhnCDE or PhnSTU. Through combining laboratory-based molecular and genetic analyses with environmental meta-omics, we identified three transporters that have a role in 2AEP uptake and revealed Pi-insensitive 2AEP catabolism is widespread in the global ocean, likely representing a major step in the marine P redox cycle.

Results

Pseudomonas putida BIRD-1 possesses a Pi-sensitive 2-aminoethylphosphonate ABC transporter, AepXVW

We recently identified several candidate 2-AEP transporters (ABC-type) in Pseudomonas rhizobacteria that contain the PhnWX phosphonatase but lack both the archetypal PhnCDE transporter and PhnSTUV44 (Fig. 1a). In Pseudomonas putida BIRD-1 (hereafter BIRD-1), a periplasmic substrate-binding protein associated with one of these putative transporters was induced under Pi-deplete growth conditions in a PhoBR-dependent manner44. We hereafter refer to this substrate-binding protein (PPUBIRD1_4925) as 2-aminoethylphosphonate X (AepX). AepX belongs to the same family (pfam13343) as PhnS, iron and sulphate substrate-binding proteins, but is clearly distinct (Coverage = 40%, Identity = 25.09%, 1.1e−05) (Fig. 1b).

BIRD-1 was capable of growth on 1.5 mM 2AEP as either a sole P, sole N or sole N and sole P source, the latter two resulting in mineralisation of excess Pi that was subsequently exported from the cell (Fig. 1c, Supplementary Fig. 1). Mutagenesis of phnWX confirmed this phosphonatase was essential for 2AEP catabolism under all growth conditions in this bacterium (Supplementary Fig. 2a, b). Next, we investigated if AepX and its corresponding ABC transporter components, the ATP binding domain protein (AepV), and the permease domain protein (AepW) were essential for its growth on 2AEP. Surprisingly, deletion of aepXVWBIRD had no effect on growth as a sole N source (Fig. 1c). However, the mutant (ΔaepXVWBIRD) had significantly (p < 0.0001) attenuated growth on 2AEP as a sole P source (Fig. 1c). The growth defect observed during growth on 2AEP as a sole P source was largely restored by complementing the mutant with a plasmid-encoded native homolog (Fig. 1c). These data suggest that whilst the AepXVW transporter is not essential, it is involved in 2AEP uptake as a sole P source in this bacterium but is not involved in growth as an N source. Therefore, another 2AEP transport system must also exist in this bacterium.

Identification of two Pi-insensitive 2-aminoethylphosphonate transporters in P. putida BIRD-1

Next, by subjecting the BIRD-1 ΔaepXVWBIRD mutant to comparative proteomics, we identified a major facilitator-type transporter, (PPUBIRD1_3129), hereafter referred to as AepP for 2-aminoethylphosphonate permease, whose expression was significantly increased during growth on 2AEP as a sole N source (Pi-insensitive) (Supplementary Fig. 3, Supplementary Table 3a). AepP is related to the glycerol-3-phosphate (G3P): Pi antiporter, GlpT45,46 (identity = 27.75%, 9e−37), a member of the organophosphate: Pi antiporter family of major facilitator transporters. AepP shares conserved residues essential for binding Pi and the Pi moiety of G3P47,48,49,50 with GlpT, whereas residues that impact the binding affinity to the glycerol moiety of G3P but not Pi are not conserved49 (Supplementary Fig. 4). Mutation of aepP in either the wild type parental strain (ΔaepP, Fig. 1d) or the aepXVW mutant (ΔaepXVW ΔaepP, Fig. 1e) led to an inability to grow on 2AEP as sole N source. Subsequent complementation of ΔaepP with its native homolog (ΔaepP + pBB:aepP) restored growth on 2AEP as a sole N source (Fig. 1d). Interestingly, delayed but significant growth on 2AEP as a P source still occurred in the ΔaepXVW ΔaepP double mutant, revealing the presence of a third 2AEP transporter in this bacterium.

To identify the unknown 2AEP transporter, we reanalysed our proteomics data (Supplementary Fig. 3). Another substrate binding protein (PPUBIRD1_3891) containing the same pfam domain (pfam13343) as AepX (Fig. 1b), hereafter named AepS, was constitutively synthesised in all growth conditions. In order to uncover the role of AepS in the utilisation of 2AEP as a sole P source, a triple mutant ΔaepXVW ΔaepP ΔaepSTU was generated in BIRD-1 (Fig. 1e). This triple knockout mutant was unable to grow on 2AEP as a sole P source (Fig. 1e), suggesting AepSTU is a functional 2AEP transporter. However, generation of a single ΔaepSTU knockout mutant did not affect Pi-sensitive growth compared to the wild type (Supplementary Fig. 2c), suggesting AepXVW is the major transporter involved in Pi-sensitive 2AEP uptake and AepSTU only has an auxiliary role in 2AEP uptake. Despite production of AepS during Pi-insensitive growth (Supplementary Fig. 3), this transporter was unable to facilitate growth on 2AEP as a sole N source in the absence of AepP. Together, these data reveal the presence of three differentially regulated 2AEP transporters in BIRD-1. AepXVW is the primary Pi-sensitive 2AEP transporter with AepSTU having an auxiliary role, whereas AepP is essential for Pi-insensitive growth on 2AEP.

AepXVW is found in several marine bacteria capable of Pi-insensitive mineralisation and is a functional 2-aminoethylphosphonate transporter

Using PPUBIRD1_4925 (AepX) as the query, we scrutinised the genomes of several isolates related to marine Rhodobacteraceae (Stappia spp., Terasakiella spp., Falsirhodobacter spp.) capable of Pi-insensitive phosphonate catabolism22. ORFs encoding orthologs of AepX were identified in the genomes of Stappia stellulata DSM 5886 and Terasakiella pusilla DSM 6293, in addition to several other marine Roseobacter strains: Aliiroseovarius crassostreae (DSM 16950), Aliiroseovarius sediminilitoris (DSM 29439), Shimia marina (DSM 26895), and Thalassobius aestuarii (DSM 15283) (Fig. 2a). We also found an orthologous ORF in the model rhizosphere alphaproteobacterium Sinorhizobium meliloti strain 1021 that is capable of 2AEP catabolism via PhnWAY31 (Fig. 2a). In all cases, ORFs encoding AepXVW were located adjacent to ORFs encoding PhnWAY or PhnWX, strongly suggesting a role in 2AEP transport. S. stellulata DSM 5886, A. crassostreae DSM 16950, A. sediminilitoris DSM 29439, S. marina DSM 26895, and T. aestuarii DSM 15283 were all capable of growth on 2AEP as either the sole N or P source (Supplementary Table 1, Supplementary Fig. 5). Indeed, both Aliiroseovarius strains lack other characterised 2AEP transport and degradation systems (Supplementary Table 2). As previously reported, Pi was exported from cells and accumulated in the medium during growth on 2AEP as a sole N source (Supplementary Fig. 6a). To confirm that S. stellulata AepXVW can take up 2AEP, we complemented the BIRD-1 null 2AEP transporter mutant (ΔaepXVW:ΔaepP:ΔaepSTU) with this transporter fused with the aepXVWBIRD promoter (Fig. 2b). This duly restored growth of the triple mutant confirming S. stellulata AepXVW is also a functional 2AEP transporter.

Fig. 2: Distribution and functional characterisation of AepXVW in marine bacteria.
figure2

a Genetic neighbourhoods of aepXVW within marine Alpha- and terrestrial Gamma-proteobacteria. Strains shown are Pseudomonas putida BIRD-1, Sinorhizobium meliloti 1021, Stappia stellulata DSM 5886, Aliiroseovarius crassostreae DSM 16950, Aliiroseovarius sediminilitoris DSM 29439, Thalassobius aestuarii DSM 15283, and Shimia marina DSM 26895. ORFs separated on the genome are indicated by breaks with the corresponding gap given in megabases (Mb). b Growth of the P. putida BIRD-1 triple 2AEP transporter mutant (ΔaepXVW:ΔaepP:ΔaepSTU) complemented with aepXVWStappia concatenated with the promoter region from aepXVWBIRD on 2AEP as either a sole N (60 h) or P (48 h) source. Data represent the mean of triplicates cultures. Error bars denote standard deviation. c Phylogeny of phosphonate-associated LysR-type regulators. Labels denote IMG/JGI gene IDs or Genbank accession numbers. Tree topology and branch lengths were calculated by maximum likelihood using the LG + G4 model of evolution for amino acid sequences based on 744 sites in IQ-TREE software84. Tree Scale represents the number of substitutions per site. A consensus tree was generated using 1000 bootstraps.

In many 2AEP gene clusters, we identified LysR-type regulators, which we refer to as AepR. A homolog of AepR has been shown to be essential for complementation of an Escherichia coli ΔphnHIJKLMNOP mutant with a Pseudomonad PhnWX25, implying substrate inducible regulation. Additionally, PhnA activity has been shown to be induced by 2AEP in a marine Falsirhodobacter isolate even under nutrient replete conditions22, though unfortunately no sequenced Falsirhodobacter strain possesses an aminophosphonate operon so it is not clear if AepR is responsible for this regulation. BIRD-1 and other Pseudomonads, whose 2AEP operons are often fragmented throughout the genome, possess up to three distinct genes encoding LysR-type regulators (Fig. 2a). In contrast, most Alphaproteobacteria only possess a single gene, located upstream of the aepXVW-phnWAY operon. These newly identified forms are phylogenetically distinct from either the archetypal PhnR51 or PalR52 found in P. fluorescens sp. 23F and Variovorax sp. PAL2, respectively (Fig. 2c). The three BIRD-1 LysR-like forms were clearly distinct from each other with the AepP-associated form being closely related to the single LysR-type regulator found in Alphaproteobacteria.

AepXVW is highly synthesised in the marine bacterium Stappia stellulata during Pi-sensitive and Pi-insensitive growth on 2-aminoethylphosphonate

In BIRD-1, AepXVW was only involved in Pi-sensitive growth whilst AepP was synthesised during Pi-insensitive and Pi-sensitive metabolism (Fig. 1). S. stellulata lacks AepP but is still capable of Pi-insensitive growth and Pi export when grown on 2AEP as a sole N, or sole N and sole P source (Supplementary Fig. 5a). In addition to aepXVW and genes encoding the 2AEP degradation system (phnWAY), S. stellulata also possesses genes (phnCDEFGHIJKLMNO) encoding the P-regulated C-P lyase operon and we also confirmed this strain grew on several other alkylphosphonates as sole P source (Supplementary Fig. 5b). Therefore, to determine which transport and degradation systems were upregulated during growth on 2AEP as either a sole N or P source, we performed comparative proteomics. Unlike BIRD-1, AepX was abundantly synthesised during growth on 2AEP as either a sole N or P source, as was PhnWAY and PbfA, the (R)-1-hydroxy-2AEP ammonia lyase, whilst the C-P lyase encoded proteins (including PhnD) were not (Fig. 3). Importantly, whilst we detected several general N stress–response proteins induced under Pi-insensitive growth, we did not identify any other potential 2AEP transporters (Supplementary Table 3b). Therefore, unlike in BIRD-1, AepXVW likely represents the major route for 2AEP uptake in this bacterium. These data are consistent with the hypothesis that S. stellulata 2AEP catabolism is regulated by a LysR-type regulator solely through substrate-induction, which will be investigated in a future study.

Fig. 3: Proteomic analysis of 2AEP-grown S. stellulata cells.
figure3

Whole-cell protein profiles (n = 3) for S. stellulata grown using either Pi or 2AEP as sole P source (a) or NH4 or 2AEP as the sole N source (b). Fold change (FC) represents the difference in Log2 Label Free Quantification (LFQ) values between each treatment and the statistical value on the Y axis is generated from Q values (FDR corrected P values). Members of the aepXVW-phnWAY operon are shown as triangles, members of the C-P lyase operon are shown as squares, all other proteins are shown as open circles. Data plotted represents the mean of triplicate cultures. Vertical dashed lines represent an Log2 LFQ difference > −3 or <3. The horizontal dashed line illustrates a cut off for a significant Q value (p < 0.05). Sky blue represents proteins showing no significant difference between treatments. Red indicates proteins significantly changing in abundance <−3-fold or >3-fold, respectively. Peach indicates significant changes less than 3-fold in either direction.

Finally, we determined the substrate specificity of recombinant S. stellulata AepX towards 2AEP and other (alkyl)phosphonates using microscale thermophoresis53,54. Unlike the relatively promiscuous phosphonate binding protein PhnD43, AepX was highly specific for 2AEP with a Kd in the nanomolar range (Table 1, Supplementary Fig. 7), consistent with the observation that aepXVW is typically co-localised with either phnWX or phnWAY that encode 2AEP-specific degradation systems (Fig. 2a).

Table 1 Microscale thermophoresis determined dissociation constants (Kd) of the S. stellulata AepX for selected phosphonate ligands.

aepX and aepP are found in distantly related and cosmopolitan bacterial taxa

Using the Integrated Microbial Genomes/Microbiomes from the Joint Genome Institute (IMG/M/JGI) database, we identified ORFs encoding AepX and AepP (but not AepS) in genomes retrieved from both taxonomically divergent isolates as well as single amplified genomes and metagenome-assembled genomes, which revealed an unexpected diversity for these substrate-binding proteins (Fig. 4). For AepX, this included cosmopolitan marine Alphaproteobacteria in addition to Rhodobacteraceae, marine Deltaproteobacteria, and marine Vibrio spp. AepX was also found in terrestrial Betaproteobacteria, Firmicutes, and other gram-positive bacteria (Fig. 4). AepX was partitioned into several subclades, with AepXStappia and AepXBIRD well separated (Fig. 4). Many taxonomically divergent AepX encoding ORFs were co-localised with ORFs encoding the various 2AEP degradation systems, including the (R)-1-hydroxy-2-aminoethylphosphonate specific PbfA, the C-P lyase, or putative uncharacterised ORFs encoding potentially novel phosphonate catabolic enzymes, supporting a role in 2AEP transport (Fig. 4).

Fig. 4: Phylogenetic and genomic analyses of AepX in marine and terrestrial bacteria.
figure4

Genetic neighbourhoods for selected AepX homologs are presented adjacent to trees. Numbers indicate environmental operational taxonomic units (OTUs) and letters indicate isolates, metagenome-assembled genomes (MAGs) or single-cell amplified genomes (SAGs). Tree topology and branch lengths were calculated by maximum likelihood using the LG + F + I + G4 model of evolution for amino acid sequences based on 744 sites in IQ-TREE software84. A consensus tree was generated using 1000 bootstraps. Branches representing isolates or MAGs/SAGs are colour coded based on their phylogenetic affiliation (see legends). Branches and identifiers for representative environmental OTU sequences (clustered at 0.8) retrieved from the TARA Oceans database are highlighted blue. The outer ring denotes the relative abundance of environmental AepX OTUs using the same colour scheme; 10% (dashed line) and 20% (filled line) thresholds are shown for scale. S. stellulata DSM 5886 and P. putida BIRD-1 AepX are labelled.

AepP was also found in a wide range of phylogenetically divergent taxa, such as Acidobacteria (Granuliella mallensis) and Bacteroidetes (Kriegella aquimaris), Actinobacteria (Streptomyces albulus CCRC 11814) and Verrucomicrobia (Haloferula sp. BvORR071 and Verrucomicrobia sp. SGGC AC-337 J20) (Supplementary Fig. 6). However unlike AepX, AepP was not found in cosmopolitan marine bacteria. Again, for all of these strains ORFs encoding AepP were co-localised with ORFs encoding PhnWAY or PhnWX, or putative catabolic enzymes (Supplementary Fig. 8). Notably, AepP was found in fewer marine isolates compared to AepX.

aepX gene and transcript abundance is far greater than the archetypal phnD/phnS in the global ocean

Using the TARA oceans OM-RGCv2 + G metagenome (MG) and OM-RGCv2 + T metatranscriptome (MT) datasets55, we calculated the abundance of aepX, aepS and aepP and compared this with phnS and the gene (phnD) encoding the archetypal phosphonate transporter, whose gene abundance in seawater was recently calculated20. The distribution of markers (phnJ, phnA, phnX) for the various phosphonate degradation mechanisms were also analysed (Supplementary Fig. 9). We analysed data from both the epipelagic and mesopelagic zones where phosphonate mineralisation is believed to occur15. Across all oceanic sampling sites in both the epipelagic and mesopelagic, aepX gene and transcript abundance was significantly greater (MG; post-hoc Dunn’s test z = 10.4, p < 0.001 and z = 4.8, p < 0.001, respectively) than phnD (Fig. 5a, b). On average, in the mesopelagic almost 10% of bacterial cells possess aepX whilst only ~0.3% and 0.5% possess phnD and aepP, respectively (Fig. 5a). The transcript abundance of aepX was 40-fold and 140-fold greater than phnD in the epipelagic and mesopelagic, respectively (Fig. 5b). The majority of aepX sequences were related to the cosmopolitan Alphaproteobacteria, although some deltaproteobacterial sequences fall within this clade (Fig. 4). We confirmed that these abundant environmental sequences were also co-localised with characterised and putative phosphonate degradation genes (Fig. 4). In broad agreement with aepX, the cumulative transcription of the two markers phnA and phnX is significantly greater than the C-P lyase marker phnJ (Kruskal–wallis Χ2 = 206.6, p < 0.001), strengthening the observation that 2AEP mineralisation is a major oceanic process (Supplementary Fig. 9). The phnZ marker is split into several subclades, with only the original PhnY*Z specific for 2AEP (Supplementary Fig. 10). This 2AEP-specific form was found at very few sites (MG = 9; MT = 2) and in low abundance in both the MG and MT (Supplementary Fig. 10). Homologs related to the two PhnZ clades associated with either methylphosphonate35,36 or (N)-trimethyl-2-aminoethylphosphonate56 degradation were found at comparable gene and transcript abundances to phnJ and significantly lower than phnA (Supplementary Fig. 11).

Fig. 5: Distribution and expression of phosphonate transporter genes in the global ocean.
figure5

Abundance (% abundance [gene or transcript] relative to the median abundance [gene or transcript] of 10 single copy core genes) of phnD, aepX, aepP in marine epipelagic (red) and mesopelagic (blue) waters, split by metagenome (MG) (a) (epipelagic: phnD n = 137, aepX n = 137, aepP n = 60, mesopelagic: phnD n = 43, aepX n = 43, aepP n = 42, where n equals the number of biologically independent sampling sites where the genes were located), and metatranscriptome (MT) (b) (epipelagic: phnD n = 148, aepX n = 154, aepP n = 11, mesopelagic: phnD n = 32, aepX n = 33, aepP n = 18, where n equals the number of biologically independent sampling sites where the transcripts were located). Abundance of phnD, aepX, aepP in MG (circles) and MT (triangles) in epipelagic (c) and mesopelagic (d) waters, split by oceanic region. aepP (red), phnD (green), aepX (blue). AO Arctic Ocean, SO Southern Ocean, IO Indian Ocean, SPO South Pacific Ocean, NPO North Pacific Ocean, SAO South Atlantic Ocean, NAO North Atlantic Ocean, RS Red Sea, MS Mediterranean Sea. Circles are mean values of Log2 abundance, error bars represent standard deviation of the mean. Epipelagic (c) phnD AO MG n = 29, MT n = 26, SO MG n = 3, MT n = 8, IO MG n = 21, MT n = 18, SPO MG n = 25, MT n = 35, NPO MG n = 11, MT n = 20, SAO MG n = 14, MT n = 17, NAO MG n = 16, MT n = 17, RS MG n = 6, MT n = 3, MS MG n = 12, MT n = 7, aepX AO MG n = 29, MT n = 28, SO MG n = 3, MT n = 8, IO MG n = 21, MT n = 19, SPO MG n = 25, MT n = 35, NPO MG n = 11, MT n = 20, SAO MG n = 14, MT n = 17, NAO MG n = 16, MT n = 17, RS MG n = 6, MT n = 3, MS MG n = 12, MT n = 7, aepP AO MG n = 11, MT n = 3, SO MG n = 0, MT n = 0, IO MG n = 10, MT n = 0, SPO MG n = 13, MT n = 4, NPO MG n = 5, MT n = 2, SAO MG n = 4, MT n = 0, NAO MG n = 11, MT n = 1, RS MG n = 4, MT n = 1, MS MG n = 2, MT n = 0. Mesopelagic (d) phnD AO MG n = 9, MT n = 7, SO MG n = 1, MT n = 0, IO MG n = 6, MT n = 4, SPO MG n = 9, MT n = 9, NPO MG n = 5, MT n = 5, SAO MG n = 5, MT n = 2, NAO MG n = 8, MT n = 5, aepX AO MG n = 9, MT n = 7, SO MG n = 1, MT n = 0, IO MG n = 6, MT n = 4, SPO MG n = 9, MT n = 9, NPO MG n = 5, MT n = 5, SAO MG n = 5, MT n = 2, NAO MG n = 8, MT n = 6, MT n = 7, aepP AO MG n = 9, MT n = 6, SO MG n = 1, MT n = 0, IO MG n = 6, MT n = 2, SPO MG n = 9, MT n = 6, NPO MG n = 5, MT n = 2, SAO MG n = 5, MT n = 1, NAO MG n = 7, MT n = 1. The relationship between the standing stock Pi concentration and transporter abundance, analysed by linear regression of Log10 Pi concentration and Log10 gene/transcript abundance, in the MG (e), (aepX R2 = 0.098, p = 1.477e-4, phnD R2 = 0.340, p = 1.349e-13, aepP R2 = 0.291, p = 1.466e-6) and MT (f), (aepX R2 = 0.007, p = 0.1544, phnD R2 = 0.203, p = 4.066e-9, aepP R2 = 0.058, p = 0.1581). aepP (red), phnD (green), aepX (blue). The relationship between R*, a measure of N vs P limitation defined as the sum of standing stock nitrate plus nitrite concentration minus 16x standing stock Pi concentration, and transporter abundance, analysed by linear regression of R* and Log10 gene/transcript abundance, in the MG (g) (aepX R2 = 0.029, p = 0.033, phnD R2 = 0.168, p = 1.611e-6, aepP R2 = 0.108, p = 5.31e-3) and MT (h) (aepX R2 = −0.005, p = 0.606, phnD R2 = 0.197, p = 1.693e-8, aepP R2 = −0.014, p = 0.402). aepP (red), phnD (green), aepX (blue). 95% confidence intervals are shown by dashed lines. In (a, b) data are represented as boxplots, where the middle line is the median and the upper and lower hinges correspond to the first and third quartiles. The upper whisker extends from the upper hinge to the largest value that is no more than 1.5 × IQR (inter-quartile range) from the upper hinge, and the lower whisker extends from the lower hinge to the smallest value that is no further than 1.5 × IQR from the lower hinge. Data beyond the ends of the whiskers are outlying points that are plotted individually. Note: aepP transcripts were not detected at numerous sites which is represented by an omission of data points.

The gene abundances of aepP, aepS and phnS were all significantly lower than both aepX and phnD (post-hoc Dunn’s test z = 13.1 and 9.0, p < 0.001, z = 14.5 and 12.6, p < 0.001, and z = 9.9 and 8.9, p < 0.001 respectively) in the epipelagic, whilst only aepS and phnS were significantly lower in the mesopelagic (post-hoc Dunn’s test z = 10.1 and 10.8, p < 0.001, and z = 4.6 and 5.5, p < 0.001 respectively) (Fig. 5a and S12).

Unlike phnD and aepP, aepX abundance was comparable across all oceanic regions within both MG and MT at each depth suggesting 2AEP mineralisation is a ubiquitous process in seawater (Fig. 5c, d). For all sites at each depth, the relative abundance of aepX transcripts was always significantly greater (Wilcoxon rank sum W = 3771, p < 0.001, estimated log2 difference = 2.24 (95% CI 1.97–2.52)) than its own gene abundance. For phnD, we observed significantly greater transcript abundance compared to its own gene abundance only in the Mediterranean Sea, a region typified by Pi-depletion (Wilcoxon rank sum W = 0, p < 0.001, estimated log2 difference = 3.11 (95% CI 1.90–4.42)). Finally, in agreement with previous work20, phnD gene abundance was inversely correlated (R2 = 0.340, p < 0.001) with standing stock concentrations of Pi (Fig. 5e), as was phnD transcript abundance (Fig. 5f). In contrast, aepX and aepP gene abundance were positively correlated (R2 = 0.098, p < 0.001 and R2 = 0.291, p < 0.001, respectively) with Pi concentration (Fig. 5e), whilst no significant relationship between Pi concentration and aepX/aepP transcript abundance was found (Fig. 5f), suggesting their expression is independent of Pi in seawater globally.

To better understand the parameters controlling 2AEP catabolism in the ocean, we compared both gene and transcript abundance in relation to R*, a measure of N vs P limitation calculated as [NO2] + [NO3] − 16[PO4] (adapted from Smith et al.57). As expected, phnD gene and transcript abundance were positively correlated with R* (R2 = 0.168, p < 0.001 and R2 = 0.197, p < 0.001, respectively) (Fig. 5g, h), i.e. regions typified by Pi depletion, though Pi concentration alone was a better predictor of phnD gene abundance (Fig. 5e). However, aepX and aepP gene abundance was (weakly) inversely correlated with R* (R2 = 0.029, p < 0.05 and R2 = 0.108, p < 0.001, respectively) (Fig. 5g) and no significant relationship was found between R* and aepX/aepP transcript abundance (Fig. 5h). These data are consistent with the proteomic response of S. stellulata under laboratory conditions and suggests AepX is induced in the presence of 2AEP (substrate-inducible) and not in response to nutrient limitation.

Discussion

Both phosphonate biosynthesis1,3,5,58 and catabolic1,23,25,31,59,60,61,62,63,64 genes are ubiquitous in marine, soil and gut microbiomes, suggesting phosphonate cycling is widespread in nature. In contrast, the uptake of these molecules is comparatively understudied, with only two characterised ABC transport systems confirmed, both of which are linked solely to P-acquisition28,42,43. The abundance of these Pi-sensitive transporters in marine systems is not equivalent to the abundance of catabolic genes20, especially those (phnWAY) recently shown to be involved in Pi-insensitive catabolism22. This would suggest our knowledge of the microbial uptake of phosphonates, particularly 2AEP, is incomplete. Using transporter expression as a proxy for the cycling of specific nutrients has significantly advanced our understanding of in situ biogeochemical cycling39,40,65. This molecular approach helped resolve the biogenesis of the climate-active gas methane in oxygenated surface waters, driven through the uptake and degradation of methylphosphonate21,38. Thus, a gap in mechanistic knowledge on 2AEP metabolism impairs our ability to survey the in situ cycling of reduced organophosphorus compounds, especially when high-resolution separation of such compounds is difficult9,66. Here, identification of Pi-insensitive 2AEP transporters allowed us to develop molecular markers to investigate the cycling of 2AEP on a global scale and compare these with previously characterised Pi-sensitive markers.

To date, whilst several ABC transporters show preference for methylphosphonate and phosphite53,67, or bind a range of phosphonates with low micromolar or lower Kd43, no ABC transporter showing a strong preference for 2AEP has been identified. Here, we revealed AepX appears to be highly specific for 2AEP and has substantially lower affinity for methylphosphonate or ethylphosphonate than PhnD43. The occurrence of aepXVW adjacent to putative phosphonate catabolic genes, and the characterised PbfA37, does suggest some degree of promiscuous binding, albeit likely to related aminophosphonates. Thus, the molecular mechanisms governing the specificity of AepX towards aminophosphonates warrant further investigation.

Genomic and biochemical analyses have revealed 2AEP and the alkylphosphonates, methylphosphonate and hydroxy-ethylphosphanate are ubiquitously synthesised in the marine environment in relatively large quantitities1,3,9,25,66. However, several studies have shown 2AEP is not detected as a significant component of ‘semi-labile’ DOM whilst alkylphosphonates tend to accumulate16,17,68. Collectively, this would suggest 2AEP is more susceptible to microbial mineralisation22 and thus shorter residence times. Here, we reveal genes for the Pi-insensitive uptake (aepX) and catabolism (phnA) are expressed at significantly higher levels across the global ocean than the Pi-repressible phnD and phnJ, providing a clear mechanism for this phenomenon. Our data also suggest 2AEP is preferentially mineralised independently of both N and P status, explaining why phosphonates are metabolised in regions where Pi or ammonium concentrations are high enough to repress C-P lyase-expression20,21,69. Together, this adds further weight to the notion that phosphonates are rapidly cycled between reduced and oxidised forms and are a source of regenerated Pi throughout the water column9,15,22.

Substrate inducible expression of catabolic genes targeting organic N molecules, irrespective of nutrient status, has previously been shown to drive mineralisation of N and cross feed into surrounding microbial cells70,71,72,73. Indeed, ammonium mineralisation may also occur if 2AEP, (R)-1-hydroxy-2-aminoethylphosphonate or (N)-trimethyl-2-aminoethylphosphonate are also used as carbon and energy sources, similar to methylamines and quaternary amines70,71. In agreement with Chin et al.22, our proteomic data for S. stellulata and in situ environmental data strongly suggests PhnA and AepX are highly synthesised in a substrate-inducible manner that would facilitate the remineralisation of labile inorganic N and P. Even if ammonium concentration does play a role in the occurrence and regulation of 2AEP degradation genes (i.e. 2AEP is primarily a N source), our combined data clearly demonstrates the potential for the in situ mineralisation of semi-recalcitrant DOP into labile Pi, a mechanism which is important for maintaining biological production in Pi-deplete regions of the ocean74,75. It should be noted that the Pseudomonas aepR located adjacent to aepP is most similar to marine aepR; this could explain why AepP was more abundant in the presence of 2AEP (Fig. 1d), although the differences in growth rate and protein abundance suggest substrate induction is not the sole mechanism of regulation in P. putida BIRD-1.

In summary, this study identified three 2AEP transporters in marine and terrestrial bacteria capable of Pi-insensitive 2AEP catabolism. The substrate-binding protein for one of these, AepX, is the most abundant and highly transcribed of the known phosphonate binding proteins in seawater. Therefore, 2AEP mineralisation may represent a major process in the marine organic N and P cycles and a significant source of regenerated labile Pi for oceanic production. This conclusion is strengthened by two key observations: (1) aepX transcription is not repressed by standing stock concentrations of Pi, and (2) extracellular Pi export occurs during Pi-insensitive 2AEP metabolism. Thus, we provide further evidence for the role of low molecular weight phosphonates acting as a P currency between autotrophic and heterotrophic microbes9.

Methods

Bacterial strains and growth conditions

All strains used in this work were axenic. Pseudomonas strains were maintained on Luria Bertani (LB) agar (1.5% w/v) medium at 30 °C. Stappia stellulata and the Roseobacter strains were maintained on Marine Broth agar (1.5% w/v) medium at 30 °C. Pseudomonas mutants and complemented strains were maintained on similar plates containing the appropriate antibiotic. For all growth and proteomics experiments cultures were grown in an adapted minimal A medium44 using Na-Succinate (20 mM) as the sole carbon source and, where applicable, 10 mM NH4Cl was added as the sole N source. 2AEP and KH2PO4 were added to a final concentration of 100 µM or 1.5 mM as specified in the text and figure legends. Pseudomonas strains were pre-cultured in minimal medium A containing 100 µM Pi and 1.5 mM NH4Cl to ensure adequate growth while minimising the potential for carryover of residual nutrients into experimental cultures. Culture experiments were performed using a FLUOStar Omega 96-well plate reader using Sarstedt 96-well plates incubated at 30°C, shaking at 200 rpm. For proteomics where 2AEP is used as an N source, S. stellulata was grown in modified defined marine ammonium mineral salt (MAMS) media lacking ammonium and with HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) replacing the Pi buffer71. For all other growth and proteomics experiments these marine bacteria were grown in Sea Salts media76, with Pi buffer replaced by HEPES buffer. The method used for quantifying extracellular Pi is detailed in the Supplementary Materials.

Generation and complementation of Pseudomonas mutants

Mutants were generated and complemented via the protocols outlined in44,77, detailed descriptions of which are outlined in the Supplementary Materials. A full list of strains, plasmids, and primers used in this study is presented in Supplementary Table 4.

Proteomics preparation and analysis

To identify proteins involved in 2AEP uptake and catabolism in S. stellulata, total protein (n = 3 for each treatment), was retrieved by sampling cell cultures (OD540 0.8–1.0) and pelleting cells (centrifugation at 16200 x g for 5 mins). Cell lysis was achieved via boiling in 100 µl lithium dodecyl sulphate (LDS) buffer (Expedeon) prior to loading 20 µl onto a 4-20% Bis-Tris sodium dodecyl sulphate (SDS) precast gel (Expedeon). For enrichment of the membrane protein fraction of the P. putida BIRD-1 ΔaepXVW mutant, we adapted the methods outlined in44. A full description of this protocol is outlined in the Supplementary Materials. Gel sections were de-stained with 50 mM ammonium bicarbonate in 50% (v/v) ethanol, dehydrated with 100% ethanol, reduced and alkylated with Tris-2-carboxyethylphosphine (TCEP) and iodoacetamide (IAA), washed with 50 mM ammonium bicarbonate in 50% (v/v) ethanol and dehydrated with 100% ethanol prior to overnight digestion with trypsin. Peptides were extracted and analysed using an Orbitrap Fusion Ultimate 3000 RSLCNano System (Thermo Scientific) in electrospray ionisation mode at the Warwick Proteomics Research Technology Platform.

Resulting tandem mass spectrometry (MS/MS) files were searched against the relevant protein sequence database (P. putida BIRD-1, NC_017530.1, S. stellulata, GCF_000423715.1) using MaxQuant78 with default settings and quantification was achieved using Label Free Quantification (LFQ). The proteomics analysis software Perseus (1.6.12)79 was used to identify differentially expressed proteins based on LFQ values, using a False Discovery Rate (FDR) of 0.01. Identified proteins were retained if they were present in at least two biological replicates within a treatment. Missing (N/A) values were imputed from a normal distribution using the default parameters. Differential expression was identified by two-sample Student’s t-test, using an s0 constant of 0.1, or ANOVA, where appropriate.

Ligand binding affinity of recombinant S. stellulata AepX

Recombinant AepX was over-produced and purified to homogeneity using methods described in53. Binding affinity was determined by microscale thermophoresis using a Monolith NT.115 instrument (NanoTemper Technologies GmbT, Germany) following the protocols described in53,54. A detailed protocol is outlined in the Supplementary Materials.

Bioinformatics analyses of aepXVW/aepP

ORFs encoding AepX homologs were identified using the IMG/JGI (https://img.jgi.doe.gov/) ‘Customised Homolog Display’ search tool. Strains containing homologs were identified (cut-off values: e−70, min. identity 40%), preferentially from type strains. In addition, representative strains from soil and marine environments were added to this list (see Supplementary Table 2). Protein sequences were aligned using ClustalOmega80 and profile Hidden Markov Models (pHMMs) were constructed from these sequences using the hmmbuild function of hmmer 3.3 (http://hmmer.org). The previously characterised E. coli K-12 PhnD81 and the SAR11 clade isolate Pelagibacter sp. HTCC7211 PhnD21 showed surprisingly low sequence homology (BLAST ID 28.46%, query coverage 76%, e-value 2e-25). We therefore, developed two pHMMS for PhnD to reflect this. There was no overlap in environmental sequences retrieved from each search using either pHMM. Therefore, abundance counts for each PhnD form were combined together as a collective PhnD group. These pHMMs were used to search the TARA ocean metagenome (OM-RGC_v2_metaG) and metatranscriptome (OM-RGC_v2_metaT) via the Ocean Gene Atlas web interface55, using a stringency of 1E-80. Sequence abundances were expressed as the average percentage of genomes containing a copy by dividing the percentage of total mapped reads by the median abundance (as a percentage of total mapped reads) of 10 single-copy marker genes82 for both MG and MT. The pHMMs were used to search the soil MG via a hmmsearch83 using the same stringency as above. Similarly, abundances were calculated as the average percentage of genomes containing a copy as above.

Phylogenetic analyses were performed using IQ-TREE 284 using the following parameters: -m TEST -bb 1000 -alrt 1000. Evolutionary relationships were inferred by maximum-likelihood analysis, and visualised using the Interactive Tree of Life (iTOL) v5.6.3 online platform (https://itol.embl.de/)85.

Statistical analysis

Unless specified above, all statistical analysis was performed using R (version 4.02)86, within the RStudio programme (version 1.3).

Reproducibility

All growth experiments were performed a minimum of two times. Proteomics experiments were performed once.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

The single-culture proteomic data generated in this study have been deposited in the ProteomeXchange Consortium via the PRoteomics IDEntifications (PRIDE) database under accession code PXD026804 [10.6019/PXD026804]. The TARA Oceans metagenomic and metatranscriptomic data used in this study are available in the European Nucleotide Archive database under accession code PRJEB7988.

References

  1. 1.

    Villarreal-Chiu, J. F., Quinn, J. P. & McGrath, J. W. The genes and enzymes of phosphonate metabolism by bacteria, and their distribution in the marine environment. Front Microbiol 3, 19 (2012).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  2. 2.

    Mukhamedova, K. S. & Glushenkova, A. I. Natural Phosphonolipids. Chem. Nat. Compd. 36, 329–341 (2000).

    CAS  Article  Google Scholar 

  3. 3.

    Yu, X. et al. Diversity and abundance of phosphonate biosynthetic genes in nature. Proc. Natl. Acad. Sci. USA 110, 20759–20764 (2013).

    ADS  CAS  PubMed  PubMed Central  Article  Google Scholar 

  4. 4.

    Ju, K.-S., Doroghazi, J. R. & Metcalf, W. W. Genomics-enabled discovery of phosphonate natural products and their biosynthetic pathways. J. Ind. Microbiol. Biotechnol. 41, 345–356 (2014).

    CAS  PubMed  Article  Google Scholar 

  5. 5.

    Peck, S. C. & van der Donk, W. A. Phosphonate biosynthesis and catabolism: a treasure trove of unusual enzymology. Curr. Opin. Chem. Biol. 17, 580–588 (2013).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  6. 6.

    Metcalf, W. W. et al. Synthesis of methylphosphonic acid by marine microbes: a source for methane in the aerobic ocean. Science 337, 1104–1107 (2012).

    ADS  CAS  PubMed  PubMed Central  Article  Google Scholar 

  7. 7.

    Born, D. A. et al. Structural basis for methylphosphonate biosynthesis. Science 358, 1336 (2017).

    ADS  CAS  PubMed  PubMed Central  Article  Google Scholar 

  8. 8.

    Hildebrand, R. The Role of Phosphonates in Living Systems. (CRC Press, 1983).

  9. 9.

    Van Mooy, B. A. S. et al. Major role of planktonic phosphate reduction in the marine phosphorus redox cycle. Science 348, 783 (2015).

    ADS  PubMed  Article  CAS  Google Scholar 

  10. 10.

    Kolowith, L. C., Ingall, E. D. & Benner, R. Composition and cycling of marine organic phosphorus. Limnol. Oceanogr. 46, 309–320 (2001).

    ADS  CAS  Article  Google Scholar 

  11. 11.

    Clark, L. L., Ingall, E. D. & Benner, R. Marine phosphorus is selectively remineralized. Nature 393, 426–426 (1998).

    ADS  CAS  Article  Google Scholar 

  12. 12.

    Turner, B. L., Baxter, R., Mahieu, N., Sjögersten, S. & Whitton, B. A. Phosphorus compounds in subarctic Fennoscandian soils at the mountain birch (Betula pubescens)—tundra ecotone. Soil Biol. Biochem 36, 815–823 (2004).

    CAS  Article  Google Scholar 

  13. 13.

    Tate, K. R. & Newman, R. H. Phosphorus fractions of a climosequence of soils in New Zealand tussock grassland. Soil Biol. Biochem 14, 191–196 (1982).

    CAS  Article  Google Scholar 

  14. 14.

    Cade-Menun, B. J., Navaratnam, J. A. & Walbridge, M. R. Characterizing dissolved and particulate phosphorus in water with 31P nuclear magnetic resonance spectroscopy. Environ. Sci. Technol. 40, 7874–7880 (2006).

    ADS  CAS  PubMed  Article  Google Scholar 

  15. 15.

    Clark, L. L., Ingall, E. D. & Benner, R. Marine organic phosphorus cycling; novel insights from nuclear magnetic resonance. Am. J. Sci. 299, 724–737 (1999).

    ADS  CAS  Article  Google Scholar 

  16. 16.

    Repeta, D. J. et al. Marine methane paradox explained by bacterial degradation of dissolved organic matter. Nat. Geosci. 9, 884–887 (2016).

    ADS  CAS  Article  Google Scholar 

  17. 17.

    Sosa, O. A. et al. Phosphonate cycling supports methane and ethylene supersaturation in the phosphate-depleted western North Atlantic Ocean. Limnol. Oceanogr. 65, 2443–2459 (2020).

    ADS  CAS  Article  Google Scholar 

  18. 18.

    Dyhrman, S. T., Benitez-Nelson, C. R., Orchard, E. D., Haley, S. T. & Pellechia, P. J. A microbial source of phosphonates in oligotrophic marine systems. Nat. Geosci. 2, 696–699 (2009).

    ADS  CAS  Article  Google Scholar 

  19. 19.

    Acker, M. et al. Phosphonate production by marine microbes: exploring new sources and potential function. bioRxiv, 2020.2011.2004.368217, https://doi.org/10.1101/2020.11.04.368217 (2020).

  20. 20.

    Sosa, O. A., Repeta, D. J., DeLong, E. F., Ashkezari, M. D. & Karl, D. M. Phosphate-limited ocean regions select for bacterial populations enriched in the carbon-phosphorus lyase pathway for phosphonate degradation. Environ. Microbiol 21, 2402–2414 (2019).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  21. 21.

    Carini, P., White, A. E., Campbell, E. O. & Giovannoni, S. J. Methane production by phosphate-starved SAR11 chemoheterotrophic marine bacteria. Nat. Comms 5, 4346 (2014).

    ADS  CAS  Article  Google Scholar 

  22. 22.

    Chin, J. P., Quinn, J. P. & McGrath, J. W. Phosphate insensitive aminophosphonate mineralisation within oceanic nutrient cycles. ISME J. 12, 973–980 (2018).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  23. 23.

    Ternan, N. G. & Quinn, J. P. Phosphate starvation-independent 2-aminoethylphosphonic acid biodegradation in a newly isolated strain of Pseudomonas putida, NG2. Syst. Appl Microbiol 21, 346–352 (1998).

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  24. 24.

    White, A. K. & Metcalf, W. W. Microbial metabolism of reduced phosphorus compounds. Annu Rev. Microbiol 61, 379–400 (2007).

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  25. 25.

    Martinez, A., Tyson, G. W. & Delong, E. F. Widespread known and novel phosphonate utilization pathways in marine bacteria revealed by functional screening and metagenomic analyses. Environ. Microbiol 12, 222–238 (2010).

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  26. 26.

    Wanner, B. L. Molecular genetics of carbon-phosphorus bond cleavage in bacteria. Biodegradation 5, 175–184 (1994).

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  27. 27.

    Kononova, S. V. & Nesmeyanova, M. A. Phosphonates and their degradation by microorganisms. Biochem. (Mosc.) 67, 184–195 (2002).

    CAS  Article  Google Scholar 

  28. 28.

    Jiang, W., Metcalf, W. W., Lee, K. S. & Wanner, B. L. Molecular cloning, mapping, and regulation of Pho regulon genes for phosphonate breakdown by the phosphonatase pathway of Salmonella typhimurium LT2. J. Bacteriol. 177, 6411 (1995).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  29. 29.

    Kim, A. D. et al. The 2-aminoethylphosphonate-specific transaminase of the 2-aminoethylphosphonate degradation pathway. J. Bacteriol. 184, 4134 (2002).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  30. 30.

    Cooley, N. A. et al. Phosphonoacetate biosynthesis: In vitro detection of a novel NADP+-dependent phosphonoacetaldehyde-oxidizing activity in cell-extracts of a marine Roseobacter. Microbiology 80, 335–340 (2011).

    CAS  Article  Google Scholar 

  31. 31.

    Borisova, S. A. et al. Genetic and biochemical characterization of a pathway for the degradation of 2-aminoethylphosphonate in Sinorhizobium meliloti 1021. J. Biol. Chem. 286, 22283–22290 (2011).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  32. 32.

    Agarwal, V. et al. Structure and function of phosphonoacetaldehyde dehydrogenase: The missing link in phosphonoacetate formation. Chem. Biol. 21, 125–135 (2014).

    CAS  PubMed  Article  Google Scholar 

  33. 33.

    McSorley, F. R. et al. PhnY and PhnZ comprise a new oxidative pathway for enzymatic cleavage of a carbon-phosphorus bond. J. Am. Chem. Soc. 134, 8364–8367 (2012).

    CAS  PubMed  Article  Google Scholar 

  34. 34.

    van Staalduinen, L. M. et al. Crystal structure of PhnZ in complex with substrate reveals a di-iron oxygenase mechanism for catabolism of organophosphonates. Proc. Natl Acad. Sci. USA 111, 5171–5176 (2014).

    ADS  PubMed  PubMed Central  Article  CAS  Google Scholar 

  35. 35.

    Sosa, O. A., Casey, J. R. & Karl, D. M. Methylphosphonate oxidation in Prochlorococcus strain MIT9301 supports phosphate acquisition, formate excretion, and carbon assimilation into purines. Appl Environ. Microbiol 85, e00289–e00219 (2019).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  36. 36.

    Gama, S. R. et al. An oxidative pathway for microbial utilization of methylphosphonic acid as a phosphate source. ACS Chem. Biol. 14, 735–741 (2019).

    CAS  PubMed  Article  Google Scholar 

  37. 37.

    Zangelmi, E. et al. Discovery of a new, recurrent enzyme in bacterial phosphonate degradation: (R)-1-hydroxy-2-aminoethylphosphonate ammonia-lyase. Biochemistry 60, 1214–1225 (2021).

  38. 38.

    Sowell, S. M. et al. Transport functions dominate the SAR11 metaproteome at low-nutrient extremes in the Sargasso Sea. ISME J 3, 93–105 (2008).

    PubMed  Article  CAS  Google Scholar 

  39. 39.

    Ottesen, E. A. et al. Pattern and synchrony of gene expression among sympatric marine microbial populations. Proc. Natl Acad. Sci. USA 110, E488–E497 (2013).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  40. 40.

    Lidbury, I., Murrell, J. C. & Chen, Y. Trimethylamine N-oxide metabolism by abundant marine heterotrophic bacteria. Proc. Natl Acad. Sci. USA 111, 2710–2715 (2014).

    ADS  CAS  PubMed  PubMed Central  Article  Google Scholar 

  41. 41.

    Mauchline, T. H. et al. Mapping the Sinorhizobium meliloti 1021 solute-binding protein-dependent transportome. Proc. Natl Acad. Sci. USA 103, 17933–17938 (2006).

    ADS  CAS  PubMed  PubMed Central  Article  Google Scholar 

  42. 42.

    Alicea, I. et al. Structure of the Escherichia coli phosphonate binding protein PhnD and rationally optimized phosphonate biosensors. J. Mol. Biol. 414, 356–369 (2011).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  43. 43.

    Rizk, S. S., Cuneo, M. J. & Hellinga, H. W. Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates. Protein Sci. 15, 1745–1751 (2006).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  44. 44.

    Lidbury, I. D. et al. Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas strains reveals novel insights into the phosphorus scavenging capabilities of soil bacteria. Environ. Microbiol 18, 3535–3549 (2016).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  45. 45.

    Lemieux, M. J., Huang, Y. & Wang, D. N. Crystal structure and mechanism of GlpT, the glycerol-3-phosphate transporter from E. coli. J. Electron Microsc 54, i43–i46 (2005). Suppl 1.

    CAS  Article  Google Scholar 

  46. 46.

    Elvin, C. M., Hardy, C. M. & Rosenberg, H. Pi exchange mediated by the GlpT-dependent sn-glycerol-3-phosphate transport system in Escherichia coli. J. Bacteriol. 161, 1054–1058 (1985).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  47. 47.

    Enkavi, G. & Tajkhorshid, E. Simulation of spontaneous substrate binding revealing the binding pathway and mechanism and initial conformational response of GlpT. Biochemistry 49, 1105–1114 (2010).

    CAS  PubMed  Article  Google Scholar 

  48. 48.

    Law, C. J. et al. Salt-bridge dynamics control substrate-induced conformational change in the membrane transporter GlpT. J. Mol. Biol. 378, 828–839 (2008).

    PubMed  Article  CAS  Google Scholar 

  49. 49.

    Law, C. J., Enkavi, G., Wang, D.-N. & Tajkhorshid, E. Structural basis of substrate selectivity in the glycerol-3-phosphate: phosphate antiporter GlpT. Biophysical J. 97, 1346–1353 (2009).

    ADS  CAS  Article  Google Scholar 

  50. 50.

    Moradi, M., Enkavi, G. & Tajkhorshid, E. Atomic-level characterization of transport cycle thermodynamics in the glycerol-3-phosphate:phosphate antiporter. Nat. Comms 6, 8393 (2015).

    ADS  CAS  Article  Google Scholar 

  51. 51.

    Kulakova, A. N. et al. Structural and functional analysis of the phosphonoacetate hydrolase (phnA) gene region in Pseudomonas fluorescens 23F. J. Bacteriol. 183, 3268 (2001).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  52. 52.

    Kulakova, A. N. et al. Expression of the phosphonoalanine-degradative gene cluster from Variovorax sp. Pal2 is induced by growth on phosphonoalanine and phosphonopyruvate. FEMS Microbiol Lett. 292, 100–106 (2009).

    CAS  PubMed  Article  Google Scholar 

  53. 53.

    Bisson, C. et al. The molecular basis of phosphite and hypophosphite recognition by ABC-transporters. Nat. Comms 8, 1746 (2017).

    ADS  Article  CAS  Google Scholar 

  54. 54.

    Adams, N. B. P., Robertson, A. J., Hunter, C. N., Hitchcock, A. & Bisson, C. Phosphite binding by the HtxB periplasmic binding protein depends on the protonation state of the ligand. Sci. Rep. 9, 10231–10231 (2019).

    ADS  PubMed  PubMed Central  Article  CAS  Google Scholar 

  55. 55.

    Villar, E. et al. The Ocean Gene Atlas: exploring the biogeography of plankton genes online. Nucleic Acids Res 46, W289–W295 (2018).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  56. 56.

    Rajakovich, L. J. et al. A new microbial pathway for organophosphonate degradation catalyzed by two previously misannotated non-heme-iron oxygenases. Biochemistry 58, 1627–1647 (2019).

    CAS  PubMed  Article  Google Scholar 

  57. 57.

    Smith, A. F. et al. Elucidation of glutamine lipid biosynthesis in marine bacteria reveals its importance under phosphorus deplete growth in Rhodobacteraceae. ISME J. 13, 39–49 (2019).

    CAS  PubMed  Article  Google Scholar 

  58. 58.

    Chin, J. P., McGrath, J. W. & Quinn, J. P. Microbial transformations in phosphonate biosynthesis and catabolism, and their importance in nutrient cycling. Curr. Opin. Chem. Biol. 31, 50–57 (2016).

    CAS  PubMed  Article  Google Scholar 

  59. 59.

    White, A. K. & Metcalf, W. W. Two C-P lyase operons in Pseudomonas stutzeri and their roles in the oxidation of phosphonates, phosphite, and hypophosphite. J. Bacteriol. 186, 4730–4739 (2004).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  60. 60.

    Ermakova, I. T. et al. Organophosphonates utilization by soil strains of Ochrobactrum anthropi and Achromobacter sp. Arch. Microbiol 199, 665–675 (2017).

    CAS  PubMed  Article  Google Scholar 

  61. 61.

    Hartley, L. E., Kaakoush, N. O., Ford, J. L., Korolik, V. & Mendz, G. L. Characterisation of Campylobacter jejuni genes potentially involved in phosphonate degradation. Gut Pathog. 1, 13 (2009).

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  62. 62.

    Imazu, K. et al. Enhanced utilization of phosphonate and phosphite by Klebsiella aerogenes. Appl Environ. Microbiol 64, 3754–3758 (1998).

    ADS  CAS  PubMed  PubMed Central  Article  Google Scholar 

  63. 63.

    Mendz, G. L., Mégraud, F. & Korolik, V. Phosphonate catabolism by Campylobacter spp. Arch. Microbiol 183, 113–120 (2005).

    CAS  PubMed  Article  Google Scholar 

  64. 64.

    Metcalf, W. W. & Wanner, B. L. Evidence for a fourteen-gene, phnC to phnP locus for phosphonate metabolism in Escherichia coli. Gene 129, 27–32 (1993).

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  65. 65.

    Hultman, J. et al. Multi-omics of permafrost, active layer and thermokarst bog soil microbiomes. Nature 521, 208–212 (2015).

    ADS  CAS  PubMed  Article  PubMed Central  Google Scholar 

  66. 66.

    Frischkorn, K. R. et al. Trichodesmium physiological ecology and phosphate reduction in the western tropical South Pacific. Biogeosciences 15, 5761–5778 (2018).

    ADS  CAS  Article  Google Scholar 

  67. 67.

    Feingersch, R. et al. Potential for phosphite and phosphonate utilization by Prochlorococcus. ISME J. 6, 827–834 (2012).

    CAS  PubMed  Article  PubMed Central  Google Scholar 

  68. 68.

    Young, C. L. & Ingall, E. D. Marine dissolved organic phosphorus composition: insights from samples recovered using combined electrodialysis/ reverse osmosis. Aquat. Geochem 16, 563–574 (2010).

    CAS  Article  Google Scholar 

  69. 69.

    Benitez-Nelson, C. R., O’Neill, L., Kolowith, L. C., Pellechia, P. & Thunell, R. Phosphonates and particulate organic phosphorus cycling in an anoxic marine basin. Limnol. Oceanogr. 49, 1593–1604 (2004).

    ADS  CAS  Article  Google Scholar 

  70. 70.

    Lidbury, I. D. E. A., Murrell, J. C. & Chen, Y. Trimethylamine and trimethylamine N-oxide are supplementary energy sources for a marine heterotrophic bacterium: implications for marine carbon and nitrogen cycling. ISME J. 9, 760–769 (2015).

    CAS  PubMed  Article  Google Scholar 

  71. 71.

    Lidbury, I., Kimberley, G., Scanlan, D. J., Murrell, J. C. & Chen, Y. Comparative genomics and mutagenesis analyses of choline metabolism in the marine Roseobacter clade. Environ. Microbiol 17, 5048–5062 (2015).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  72. 72.

    Zecher, K., Hayes, K. R. & Philipp, B. Evidence of interdomain ammonium cross-feeding from methylamine- and glycine betaine-degrading Rhodobacteraceae to diatoms as a widespread interaction in the marine phycosphere. Front Microbiol 11, 2431 (2020).

    Article  Google Scholar 

  73. 73.

    Christie-Oleza, J. A., Sousoni, D., Lloyd, M., Armengaud, J. & Scanlan, D. J. Nutrient recycling facilitates long-term stability of marine microbial phototroph–heterotroph interactions. Nat. Microbiol 2, 17100 (2017).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  74. 74.

    Dyhrman, S. T. et al. Phosphonate utilization by the globally important marine diazotroph Trichodesmium. Nature 439, 68–71 (2006).

    ADS  CAS  PubMed  Article  Google Scholar 

  75. 75.

    Karl, D. M. et al. Aerobic production of methane in the sea. Nat. Geosci. 1, 473–478 (2008).

    ADS  CAS  Article  Google Scholar 

  76. 76.

    Chen, Y. Comparative genomics of methylated amine utilization by marine Roseobacter clade bacteria and development of functional gene markers (tmm, gmaS). Environ. Microbiol 14, 2308–2322 (2012).

    CAS  PubMed  Article  Google Scholar 

  77. 77.

    Lidbury, I. D. E. A. et al. Identification of extracellular glycerophosphodiesterases in Pseudomonas and their role in soil organic phosphorus remineralisation. Sci. Rep. 7, 2179 (2017).

    ADS  PubMed  PubMed Central  Article  CAS  Google Scholar 

  78. 78.

    Tyanova, S., Temu, T. & Cox, J. The MaxQuant computational platform for mass spectrometry-based shotgun proteomics. Nat. Protoc. 11, 2301–2319 (2016).

    CAS  PubMed  Article  Google Scholar 

  79. 79.

    Tyanova, S. et al. The Perseus computational platform for comprehensive analysis of (prote)omics data. Nat. Methods 13, 731–740 (2016).

    CAS  PubMed  Article  Google Scholar 

  80. 80.

    Madeira, F. et al. The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res 47, W636–W641 (2019).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  81. 81.

    Finn, R. D., Clements, J. & Eddy, S. R. HMMER web server: interactive sequence similarity searching. Nucleic Acids Res 39, W29–W37 (2011).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  82. 82.

    Wackett, L. P., Wanner, B. L., Venditti, C. P. & Walsh, C. T. Involvement of the phosphate regulon and the psiD locus in carbon-phosphorus lyase activity of Escherichia coli K-12. J. Bacteriol. 169, 1753–1756 (1987).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  83. 83.

    Milanese, A. et al. Microbial abundance, activity and population genomic profiling with mOTUs2. Nat. Comms 10, 1014 (2019).

    ADS  Article  CAS  Google Scholar 

  84. 84.

    Minh, B. Q. et al. IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Mol. Biol. Evol. 37, 1530–1534 (2020).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  85. 85.

    Letunic, I. & Bork, P. Interactive Tree Of Life (iTOL) v4: recent updates and new developments. Nucleic Acids Res 47, W256–W259 (2019).

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  86. 86.

    R. Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, https://www.R-project.org/ (2020).

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Acknowledgements

We thank the Warwick Proteomics Research Facility, namely Dr. Cleidiane Zampronio for her assistance in generating and processing the mass-spectrometry data. This study was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) under project codes BB/L026074/1, BB/T009152/1 and NE/S013539/1 linked to The Soil and Rhizosphere Interactions for Sustainable Agri-ecosystems (SARISA) programme and a Discovery Fellowship (IL) and NERC Environmental ‘Omics Synthesis Grant (IL and EW), respectively.

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A.M. and I.L. wrote the manuscript with comprehensive feedback and guidance from Y.C. and D.S. A.M. performed the experimental work, and A.M. and I.L. analysed the data, except for the binding affinity assays, performed and analysed by N.A., W.C., A.H. and I.L. I.L. and A.M. conceptualised and developed the research, respectively. A.B. provided input and support for proteomic data generation and analyses. All authors contributed to revisions of the manuscript.

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Correspondence to Ian D. E. A. Lidbury.

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Murphy, A.R.J., Scanlan, D.J., Chen, Y. et al. Transporter characterisation reveals aminoethylphosphonate mineralisation as a key step in the marine phosphorus redox cycle. Nat Commun 12, 4554 (2021). https://doi.org/10.1038/s41467-021-24646-z

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