Fig. 1: Glucocorticoid receptor induces cell dormancy in lung cancer models. | Nature Communications

Fig. 1: Glucocorticoid receptor induces cell dormancy in lung cancer models.

From: Glucocorticoid receptor triggers a reversible drug-tolerant dormancy state with acquired therapeutic vulnerabilities in lung cancer

Fig. 1

a Representative western blot showing expression of GR, with actin as a loading control (n = 2). b Doubling time (in hours) as calculated from real-time proliferation data of cells treated with vehicle (Veh) or glucocorticoids (GC). Bars depict mean value ± SEM of independent doubling time calculations (A549 n = 18, H2122 n = 18, H1944 n = 18, H1975 n = 24, H460 n = 18). P values were determined by two-sided Mann–Whitney test. c All GC-induced dephosphorylation events captured by mass spectrometry across A549, H2122 and H1944, relative to untreated condition (n = 3). P values were determined by two-sided t test. d Gene-set-enrichment analysis (GSEA) of dephosphorylated events discovered by mass spectrometry analysis performed in A549 (blue), H2122 (red) and H1944 (green) cell lines (n = 3). e E2F target gene-set (M5925) GSEA enrichment profiles for whole-proteome mass spectrometry experiments performed in A549 (blue), H2122 (red) and H1944 (green) cell lines (n = 3). Nominal P values were determined by GSEA software. f Fridman senescence gene-set (M9143) GSEA enrichment profiles for whole-proteome mass spectrometry experiments performed in A549 (blue), H2122 (red), and H1944 (green) cell lines (n = 3). Nominal P values were determined by GSEA software. g Representative images of senescence-associated β-galactosidase (X-gal) stained cells, untreated (Veh) or glucocorticoid-treated (GC) (n = 3). Scale bar, 10 μm. h Quantification of at least 200 cells from senescence-associated β-galactosidase experiments represented in fraction of negative (grey) and positive cells (blue) (n = 3). i Per DNA content normalised oxygen consumption rate (OCR) for A549, H2122 and H1944, without (Veh) or with glucocorticoids (GC). Bars depict mean values ± SEM of independent experiments (A549 n = 3, H2122 n = 3, H1944 n = 2). P values were determined by two-sided Mann–Whitney U test. j Per DNA content normalised extracellular acidification rate (ECAR) for A549, H2122 and H1944, without (Veh) or with glucocorticoids (GC). Bars depict mean values ± SEM of independent experiments (A549 n = 3, H2122 n = 3, H1944 n = 2). P values were determined by two-sided Mann–Whitney U test. Source data are provided as a Source Data file.

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