Tuberous Sclerosis Complex (TSC) is caused by TSC1 or TSC2 mutations, resulting in hyperactivation of the mechanistic target of rapamycin complex 1 (mTORC1). Transcription factor EB (TFEB), a master regulator of lysosome biogenesis, is negatively regulated by mTORC1 through a RAG GTPase-dependent phosphorylation. Here we show that lysosomal biogenesis is increased in TSC-associated renal tumors, pulmonary lymphangioleiomyomatosis, kidneys from Tsc2+/− mice, and TSC1/2-deficient cells via a TFEB-dependent mechanism. Interestingly, in TSC1/2-deficient cells, TFEB is hypo-phosphorylated at mTORC1-dependent sites, indicating that mTORC1 is unable to phosphorylate TFEB in the absence of the TSC1/2 complex. Importantly, overexpression of folliculin (FLCN), a GTPase activating protein for RAGC, increases TFEB phosphorylation at the mTORC1 sites in TSC2-deficient cells. Overexpression of constitutively active RAGC is sufficient to relocalize TFEB to the cytoplasm. These findings establish the TSC proteins as critical regulators of lysosomal biogenesis via TFEB and RAGC and identify TFEB as a driver of the proliferation of TSC2-deficient cells.
Tuberous sclerosis complex (TSC) is caused by mutational inactivation of the TSC1 or TSC2 tumor suppressor genes1. TSC affects multiple organs including the brain, heart, kidney, lung and skin2. While the majority of tumors in TSC are benign, malignant tumors also occur, particularly in the kidney3. TSC1 and TSC2 are part of the protein complex that integrates signals from the extracellular environment (oxygen, energy, nutrients, growth factors) to regulate the kinase activity of mechanistic/mammalian target of rapamycin complex 1 (mTORC1) via the small GTPase Ras homolog enriched in brain (RHEB)4,5,6,7,8. RHEB-dependent mTORC1 activation takes place on the surface of the lysosome9. The hallmark of TSC1 and TSC2-deficient cells (hereafter referred to as TSC-deficient cells) is therefore hyperactivation of mTORC1, which is believed to be the primary driver of tumorigenesis in TSC.
TFEB (transcription factor EB) and the other members of the MiTF family of transcription factors (MITF, TFE3, TFEC) are master regulators of lysosomal gene expression, lysosomal biogenesis, and autophagy10,11,12,13,14. The localization and function of TFEB are tightly regulated by mTORC1 kinase activity, with phosphorylation of TFEB resulting in cytoplasmic sequestration by 14-3-3 proteins13,15,16,17.
Recruitment of mTORC1 to lysosomes occurs via interactions with the Ras-related GTP-binding proteins (RAG GTPases), which are activated when amino acids are abundant18. In order to be phosphorylated by mTORC1, TFEB needs also to be recruited to lysosomes by the RAG GTPases19. Folliculin (FLCN) is a GTPase activating protein for RAGC/D19,20,21,22 and thereby regulates both mTORC1 and TFEB abundance at the lysosomal surface19,23,24. Germline mutations in FLCN cause the hereditary cancer syndrome Birt-Hogg-Dube (BHD)25,26, which shares some clinical phenotypes with TSC including benign skin tumors, cystic lung disease, and renal cell carcinoma (RCC).
Because mTORC1 is hyperactive in TSC, TFEB should be predominantly cytoplasmic in TSC-deficient cells. However, prior studies in TSC-deficient cells have revealed conflicting results in terms of TFEB localization13,15,24,27,28,29. Here we demonstrate that despite high mTORC1 activity in TSC-deficient cells, TFEB is predominantly nuclear and unexpectedly hypo-phosphorylated at the mTORC1-dependent sites. Furthermore, TFEB drives lysosomal gene expression and promotes proliferation in vitro and in vivo in TSC2-deficient cells. It has recently been discovered that renal tumorigenesis in BHD is TFEB-dependent24. This supports the concept that the regulation of TFEB is the critical mechanistic link between tumorigenesis in TSC and BHD, diseases in which there is some clinical similarity, and further highlights the possibility that TFEB may be a primary driver of tumorigenesis in TSC. Taken together, our findings indicate that TFEB is a critical disease-relevant target of the TSC proteins.
Lysosome abundance, lysosomal gene expression, and protein levels are increased in TSC
Renal disease is a major source of morbidity and mortality in TSC30,31. To elucidate the pathogenesis of renal lesions in TSC, transmission electron microscopy (TEM) was performed on the kidneys of 18-months old Tsc2+/− mice, which develop renal cysts and cystadenomas32. This revealed a 3-fold increase in lysosome number within cystic epithelial cells compared to normal adjacent kidney (Fig. 1a, b). Consistent with this increased lysosomal number, expression of the lysosomal cholesterol transporter Niemann-Pick C1 (NPC1)33, was enriched in the mouse kidney cystic epithelium (Fig. 1c). Interestingly, NPC1 is also enriched in renal lesions from TSC patients (angiomyolipomas and RCCs) compared with adjacent normal kidney (Fig. 1d–f, Supplementary Fig. 1a). In Tsc1−/− and Tsc2−/− mouse embryonic fibroblasts (MEFs), mRNA levels of lysosomal genes such as Npc1, Niemann-pick c2 (Npc2), Cathepsin K (Ctsk), and Hexosaminidase (Hexa) were increased 2- to 6-fold compared to controls (Fig. 1g, h), and protein levels of NPC1 and Cathepsin K were higher in Tsc2−/− MEFs compared with controls (Fig. 1i). These results establish that lysosomes, which are increasingly recognized as critical drivers of tumorigenesis34,35,36, are enriched in TSC.
TSC-deficiency leads to increased nuclear localization and activity of TFEB with decreased TFEB phosphorylation at Serine 142 and 211
To understand the mechanisms driving high lysosome abundance in TSC, we focused on the MiTF family of transcription factors (TFEB, TFE3, MITF, TFEC), known regulators of lysosome biogenesis10,11,13. We found that Tfeb, but not Tfe3 or Mitf, was upregulated in Tsc-deficient MEFs (Supplementary Fig. 1b). Tfec was not expressed. As noted earlier, although phosphorylation of TFEB by mTORC1 is known to result in its cytoplasmic sequestration13,15,16,17, the localization of TFEB in TSC-deficient cells is controversial15,16,24,27,29. We found that TFEB is predominantly nuclear in Human Melanoma Black-45 (HMB45) and NPC1-positive lymphangioleiomyomatosis (LAM) nodules, the pulmonary manifestation of TSC37 (Supplementary Fig. 1c–e). TFEB is also primarily nuclear in TSC-associated RCC and renal angiomyolipomas (Fig. 2a–c), and in Tsc1/2-null MEFs (Supplementary Fig. 2a).
To understand the mechanisms through which TFEB is nuclear in TSC, despite high mTORC1 activity, we used HeLa cells with stable expression of TFEB-GFP13,15,16,17. First, we confirmed the nuclear enrichment of exogenous GFP-tagged TFEB after either TSC1 or TSC2 knockdown by siRNA (Fig. 2d, e). Next, we examined TFEB’s phosphorylation at the mTORC1-dependent sites (S142 and S211). Surprisingly, both mTORC1-dependent sites were hypophosphorylated in TSC-deficient HeLa-TFEB-GFP cells (Fig. 2f, g).
To determine if the nuclear TFEB in TSC2-deficient cells is active, we generated a novel luciferase construct derived from the promoter of the transmembrane glycoprotein NMB (GPNMB), a TFE3-TFEB target, and lysosomal glycoprotein38. TSC2 downregulation by siRNA increased the activity of the GPNMB promoter by 10-fold in HeLa cells and 27-fold in HeLa-TFEB-GFP cells (Fig. 2h). Downregulation of FLCN, used as positive control, increased the promoter activity of GPNMB by 7-fold in HeLa cells and 10-fold in HeLa-TFEB-GFP cells (Fig. 2h).
In Tsc1-null and Tsc2-null MEFs we observed increased Tfeb mRNA expression (Supplementary Fig. 1b), thereby in these cell lines increased nuclear localization and activity of TFEB could be due to its higher total levels. In HEK293T cells after TSC2 knockdown by siRNA we found only a minor increase in TFEB mRNA expression (20%) (Fig. 3a), no appreciable change at the protein level (Fig. 3b), and primarily nuclear TFEB (Fig. 3c). Similarly, in HeLa cells after TSC2 knockout by CRISPR we observed a 50% increase in the mRNA expression of TFEB (Fig. 3d), no appreciable change at the protein level (Fig. 3e), and a clear increase in nuclear TFEB after nuclear/cytoplasmic fractionation (Fig. 3f), indicating that increased nuclear localization of TFEB is not driven by increased expression.
TFE3 was also primarily nuclear in TSC-deficient cells (Fig. 3c, f, Supplementary Fig. 2b, c) behaving similarly to TFEB, TFE3 mRNA, and protein expression was not affected by TSC2 loss. Overexpression of TFEB with serine to alanine mutation at the mTORC1-dependent phosphorylation sites (S142, S211, and S142/S211) resulted in nuclear localization of TFEB in both TSC2-expressing and TSC2-deficient HeLa cells (Supplementary Fig. 3).
To confirm that increased GPNMB promoter activity and increased lysosomal gene expression are TFEB-dependent in TSC2-deficient cells, we downregulated TFEB by siRNA and observed decreased GPNMB promoter activity (Supplementary Fig. 4a), GPNMB protein expression (Supplementary Fig. 4b), as well as decreased expression of multiple lysosomal genes including GPNMB, ATPase H + transporting VO subunit D2 (ATP6V0D2), Interleukin 33 (IL-33) NPC1 and Mucolipin1 (MCOLN1) (Supplementary Fig. 4c).
Tfeb knockdown decreases proliferation of Tsc2-deficient cells
To understand better the functions of TFEB in TSC, we knocked down Tfeb in Tsc2-expressing and deficient MEFs with two different shRNAs (Fig. 4a). Downregulation of Tfeb decreased the proliferation of Tsc2−/− MEFs, with a 25% decrease for shTfeb #1 and a 45% decrease for shTfeb#2 at 72 h (Fig. 4b). Tfeb downregulation did not affect the growth of Tsc2+/+ MEFs (Fig. 4b). Rapamycin (20 nM) had no additional effect on the proliferation of Tsc2-deficient cells with Tfeb downregulation (Supplementary Fig. 5a), although it decreased the expression of Tfeb by about 2-fold (Supplementary Fig. 5b). In vivo, the subcutaneous growth of Tsc2-null MEFs was decreased by 3-fold in cells with Tfeb downregulation (Fig. 4c). Taken together these data establish Tfeb as a driver of Tsc2-deficient cell proliferation in vitro and in vivo.
FLCN and TSC2 coordinately regulate TFEB localization, activity, and phosphorylation
FLCN is a GAP for RAGC/D, converting RAGC/D from the inactive (GTP)-bound to the active (GDP)-bound form, thereby facilitating the recruitment of TFEB to the surface of the lysosome where it can be phosphorylated by mTORC119,20,21,22,23,39. Interestingly, we found that FLCN expression is increased ~2-fold upon TSC2 downregulation in Hela and Hela-TFEB-GFP cells (Fig. 5a). To examine the relationship between TSC2 and FLCN, TSC2 and FLCN were downregulated alone and in combination in HeLa, and HeLa TFEB-GFP cells using siRNA. The combined downregulation of TSC2 and FLCN resulted in stronger nuclear localization of TFEB-GFP (Fig. 5b, c), a further increase in GPNMB activity (Fig. 5d), a further decrease in S211 phosphorylation (Fig. 5e, f), and a higher expression of lysosomal genes when compared to single gene knockdowns of either TSC2 or FLCN (Fig. 5g). In parallel, we found that overexpression of FLCN (Myc-FLCN) partially restored TFEB phosphorylation at S142 and S211 in HEK293T cells after TSC2 downregulation by siRNA (Fig. 5h, i). Further work using CRISPR-mediated knockout of TSC2 and FLCN will be important to complement these siRNA-based findings.
Activation of RAGC GTPase is necessary and sufficient to localize TFEB into the cytoplasm in TSC2-deficient cells
Our data showing that knockdown of either FLCN or TSC2 has similar effects on TFEB phosphorylation, localization, and activity led us to focus on the RAG GTPases in TSC2-deficient cells. We found that RAGC and RAGD were increased at the mRNA level in HeLa cells after knockdown of either TSC2 or FLCN, and even further increased with downregulation of both TSC2 and FLCN (Supplementary Fig. 6a). In HeLa-TFEB-GFP (which do not express detectable level of RAGD), a similar pattern was found for the expression of RAGC at the mRNA and protein levels (Supplementary Fig. 6b, c). Interestingly, RAGC/D have been shown to be TFEB targets40.
The increase in RAGC/D expression in TSC2-deficient cells, together with the increase in FLCN expression, suggest a potential role of the RAG GTPases in the nuclear localization of TFEB in TSC. To determine if the localization of TFEB in TSC2-deficient cells is RAG-dependent we overexpressed wild-type RAGA/C and constitutively active (CA) RAGA/C (RAGAQ66L-GTP bound, RAGC S75N-GDP bound)41 in HeLa-TFEB-GFP cells with TSC2-downregulation. CA active RAGA/C, but not wild-type RAGA/C, decreased the nuclear localization of TFEB in HeLa-TFEB-GFP cells with TSC2-downregulation (Fig. 6a). To dissect which component of the RAG heterodimer was primarily responsible for the decreased nuclear localization of TFEB-GFP, we individually expressed wild-type (WT) RAG A, WT RAG C, CA RAG A, and CA RAG C. CA RAGC (but not WT RAGA, WT RAGC or CA RAGA) decreased nuclear TFEB-GFP levels (Fig. 6b, Supplementary Fig. 7a, b). CA RAGC increased the levels of TFEB phosphorylation at S211 in cells with TSC2 downregulation (Fig. 6c) and CA RAGC alone was as efficient as the CA RAGA/C combination in relocalizing TFEB-GFP to the cytoplasm (Supplementary Fig. 8a, b) and in increasing TFEB phosphorylation at S142/211 (Supplementary Fig. 8c). Treatment with Torin1, an mTOR kinase inhibitor (250 nM, 6 h), resulted in nuclear localization of TFEB in both siCtrl and siTSC2 HeLa-TFEB-GFP cells expressing CA RAGC (Supplementary Fig. 9). These data indicate that the localization of TFEB in TSC2-deficient cells is both RAGC and mTOR-dependent (Fig. 6d).
In this study we demonstrate that TFEB is predominantly nuclear in human TSC lesions and in TSC-deficient cells with both acute and chronic downregulation of TSC2 and TSC1, where it drives lysosomal biogenesis and cell growth in vitro and in vivo. In TSC-deficient cells, TFEB is hypo-phosphorylated at the mTORC1-dependent sites. TFEB’s nuclear localization and hypo-phosphorylation in TSC are unexpected because TSC-deficient cells have high mTORC1 activity, and phosphorylation of TFEB by mTORC1 is a well-established mechanism of TFEB’s cytoplasmic sequestration13,15,16,17. Prior analyses of TFEB localization in TSC2-deficient cells have shown variable results, with some studies showing primarily nuclear localization28,29 and others primarily cytoplasmic localization15,24,27. Of note, the prior studies focused on cultured cell models, while our work included also mouse and human tumor specimens of TSC, cellular models of acute and chronic loss of TSC2, and multiple methods of TSC2 downregulation (littermate-derived Tsc2+/+ and Tsc2−/− MEFs, siRNA, and CRISPR/Cas9 downregulation of TSC2). The reasons for the differing results are unclear at this time but could reflect differences in nutrient conditions and/or the duration and extent of TSC2 downregulation. Taken together, our data indicate that a non-canonical regulatory mechanism is responsible for TFEB’s nuclear localization in TSC.
Lysosomes are an emerging driver of tumorigenesis34,35,36. TFEB and TFE3 are oncogenes, with translocations involving splicing genes and TFE3 (or TFEB) causing a particularly aggressive RCC that disproportionally affects children and young adults42,43. These translocation RCC, which were previously referred to as “TSC-like”42, may reflect key similarities to RCC in TSC patients3,44, in which we have found high levels of nuclear TFEB and high expression of the lysosomal protein NPC1. Moreover, TFEB has been shown to be a primary driver of pancreatic adenocarcinoma45. Our data support the concept that activation of TFEB is a key driver of renal tumorigenesis in TSC.
TFEB and its family members TFE3 and MITF may also be involved in other manifestations of TSC via enhanced lysosomal biogenesis and/or other mechanisms. Cathepsin K (a lysosomal enzyme) is known to be upregulated in pulmonary LAM46 and multiple lysosomal genes are increased in TSC-associated subependymal giant cell astrocytomas compared with normal brain47. The hypothesis that TFEB and lysosomes are directly involved in the pathogenesis of TSC via a non-canonical RAGC-dependent mechanism challenges the concept that hyperactivity of mTORC1 to its canonical substrates is the unique driver of tumor formation in TSC.
Increased nuclear TFEB is a hallmark of BHD syndrome24, which has some clinical similarity to TSC (both diseases are associated with chromophobe/oncocytic RCCs, benign facial skin tumors, and cystic lung disease)48,49. BHD is caused by mutations in FLCN, a known GAP for RAGC/D20,21,22. Levels of mTORC1 activity vary considerably between different in vitro and in vivo models of BHD, with some FLCN-deficient cells showing lower mTORC1 activity50,51 and others showing higher mTORC1 activity24,52,53. As a GAP for RAGC/D, FLCN knockdown would be predicted to result in lower mTORC1 activity51, on the other hand, FLCN loss has been shown to increase the expression of RAGD through TFEB and thereby boost RAG GTPase-dependent mTORC1 activation40, in fact hyperactive mTORC1 has been observed in BHD-associated RCC54. We found increased expression of RAGC and RAGD in both TSC2 and FLCN-deficient HeLa cells, with an even greater increase in RAGC/D expression in cells with downregulation of both TSC2 and FLCN. The increase in RAGC/D expression in TSC2-deficient cells could represent an additional mechanism by which the absence of TSC2 sustains high mTORC1 activity, similarly to what has been proposed for FLCN loss40,55.
Our discovery that constitutively active RAGC, but not wild-type RAGC, is sufficient to induce cytoplasmic TFEB localization in TSC2-deficient cells further supports a model in which FLCN and TSC2 act in parallel to regulate TFEB via the activity of RAGC/D. It is possible that in a TSC-deficient setting, impaired RAGC/D activity is responsible for the inability of mTORC1 to phosphorylate TFEB, as has been shown for FLCN-deficient cells20,24,56. Moreover, the fact that combined loss of FLCN and TSC2 induced even stronger nuclear localization of TFEB, while overexpression of FLCN in TSC2-deficient cells increased the phosphorylation of TFEB at the mTORC1 sites, support a pathogenic link between TSC and BHD. Further work will be needed to determine if Tfeb inactivation can alleviate renal disease in genetically engineered mouse models of TSC, as has been recently demonstrated for BHD-associated renal disease24.
In summary, we identified a non-canonical RAGC-dependent pathway through which loss of the TSC complex drives TFEB into the nucleus and increases lysosomal gene expression and cell proliferation. TFEB represents a previously unrecognized pathogenic link between the clinical manifestations of TSC and BHD and may represent a therapeutic target for the treatment of both diseases.
Tsc1+/+, Tsc1−/−, Tsc2+/+, Tsc2−/− MEFs were provided by Dr. David Kwiatkowski at Brigham and Women’s Hospital Boston, MA, US. HeLa cells and HEK293T cells were purchased from ATCC. Hela-TFEB-GFP cells were developed by Shawn Ferguson16. All cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Gibco/Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum with 1% penicillin/streptomycin, with the exception of the Tsc2+/+ and Tsc2−/− MEFs in Fig.1h and i, which were grown in 0.1% FBS for 96 h. Rapamycin R-5000 (20 nM) and Torin1 T-7887 (250 nM) were purchased from LC Laboratories.
Generation of TFEB knockdown cell lines
Tfeb mouse shRNA Lentiviral Transduction Particles (Sigma-Aldrich, clone TRCN0000085548 and Dharmacon, clone V3SVMM00_13136092) were used to downregulate Tfeb in Tsc2+/+ and Tsc2−/− MEFs. Cells were transduced with Lentiviral particles for 24 h with polybrene (8 µg/ml) and selected with puromycin at 5 µg/ml.
Generation of HeLa TSC2 CRISPR knock-out line
Non-targeting control gRNA and TSC2 gRNA plasmids were designed using pRP_gRNA_Cas9 plasmid backbone from Vector Builder. The expression of small guide RNAs and mCherry-tagged human codon-optimized Cas9 from Streptococcus pyogenes was driven by U6 and modified chicken beta-actin (CBh) promoters respectively. Both non-targeting control and TSC2 CRISPR plasmids included two small guide RNAs (GTGTAGTTCGACCATTCGTG and GTTCAGGATCACGTTACCGC for non-targeting control; TCCTTGCGATGTACTCGTCG and GACCCGGTCGTTACTAGGCC for TSC2). Plasmids were transiently expressed in HeLa cells using Lipofectamine 3000 (Life Technologies/Thermo Fisher) for 48 h and single-cell sorted for mCherry on 96-well plates. Single Clones were isolated and TSC2 knock-out was verified by immunoblotting.
The following siRNA reagents were used: TSC2 Silencer Select siRNA (ThermoFisher, assay ID s502596), TSC1 Silencer Select siRNA (Thermofisher, assay ID s526384), FLCN Silencer Select siRNA (ThermoFisher, assay ID s47320), TFEB Silencer Select siRNA (ThermoFisher, assay ID s15496) and non-targeting control Silencer Select siRNA (ThermoFisher, 4390844). All siRNA transfections were performed using Lipofectamine RNAiMax (ThermoFisher, LMRNA015).
Cell viability was assessed using crystal violet staining. One thousand cells were seeded on clear 96-well plates. Cells treated with Dmso or Rapamycin (20 nM) were fixed after 24, 48, 72 h with 10% formalin for 10 min and stained with 500 mg/L crystal violet (Sigma) solution for 20 min. Crystal violet was then eluted with methanol for 5 min in a plate shaker. Absorbance was measured at 540 nm.
Animal studies were approved by the Brigham and Women’s Hospital Animal Care and Use Committee (IACUC) and conducted according to an approved protocol for the care and maintenance of laboratory animals. Mice were housed in an animal facility with 12 h light/12 h dark cycle at 72 °F and 40% humidity with unrestricted access to food and water. 18 months old female AJ Tsc2+/− mice were used for electron microscopy of the kidneys. Xenografts tumors were generated by subcutaneously injecting (2.0 × 106) Tsc2−/− MEFs mixed with matrigel 1:1 (v/v) (Corning, 356237) to a final volume of 100–150 μl unilaterally into the shoulders of 8-week-old female NOD-scid IL2Rgammanull anesthetized mice using a 21 G needle. Mice were inspected weekly, and tumors were measured every three days by caliper once they became palpable and >100 m3.
Kidney specimens from Tsc2+/− mice in the AJ genetic background at 18 months of age were fixed in 2% glutaraldehyde/2% paraformaldehyde in 0.1 M phosphate buffer for 3 h at 4 °C, postfixed in 1% osmium tetroxide in the same buffer solution, dehydrated in graded alcohols, and embedded in an Epon-Araldite mixture. Thin sections were stained with lead citrate and examined with a CM10 transmission electron microscope. Ten random images at the same magnification for each sample were utilized for lysosome quantitation in either cysts or normal adjacent kidney tissue.
Protein extraction and western blot analysis
Cells were washed with ice-cold PBS, scraped, and lysed on ice with 1× RIPA buffer. Lysates were normalized by concentration and each sample resolved on 4–12% Bis-Tris gel. Chemiluminescence was visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA, USA). To isolate the nuclear and the cytoplasmic protein fraction, CelLytic NuCLEAR Extraction Kit was used (Millipore Sigma, NACRES NA56). The following antibodies were used at 1:1000 dilution unless otherwise indicated: β-actin (Sigma-Aldrich, A5316), phosphorylated S6 ribosomal protein (pS6 Ser 235/236) (Cell Signaling Technology, 2211), total S6 ribosomal protein (TS6) (Cell Signaling Technology, 2217), phosphorylated 4E-BP1 (p4E-BP1 Thrn37/46) (Cell Signaling Technology, 2855), total 4E-BP1 (T4E-BP1) (Cell Signaling Technology, 9644), TSC2 (Cell Signaling Technology, 4308), TSC1 (Cell Signaling Technology, 4906), TFEB (1:3000) (Bethyl Laboratories, A303-673A), TFEB (Cell Signaling Technology, 32361), TFE3 (Cell Signaling Technology, 14779), Cathepsin K (CTS K) (Abcam, ab19027), NPC1 (Abcam, ab134113), FLCN (Cell Signaling Technology, 3697), GFP (1:5000) (Abcam, ab13970), phosphorylated TFEB S211 (Cell Signaling Technology, 37681), phosphorylated TFEB S142 (Millipore Sigma, ABE1971), RAGC (Cell Signaling Technology, 9480), MYC-Tag (Cell Signaling Technology, 2276), HA-tag (Cell Signaling Technology Inc., 3724S), GPNMB (Cell Signaling Technology, 38313), CREB (Cell Signaling Technology, 9197), GAPDH (Cell Signaling Technology, 5174).
The following plasmids were used in HeLa-TFEB-GFP cells: pRK5 HA- RAGA (#99710), pRK5-HA GST RAGA 66L (#19300), pRK5 HA- RAGC (#99718), and pRK5-HA GST RAGC 75L (#19305), were purchased from Addgene. The following plasmids were used in HeLa cells: TFEB-GFP wild type, TFEB-GFP S142A, TFEB-GFP S211A, TFEB-GFP S142A/S211A developed by Dr. Shawn Ferguson. The following plasmids were used in HEK293T cells: pEGFP-N1-TFEB (Addgene #38119), pCMV-Tag3B vector plasmid (Agilent) and pCMV-Tag3B expressing myc-FLCN plasmid57. All plasmids were expressed using Fugene6 reagent (Promega) for 48 h in immunofluorescence experiments and using Lipofectamine 3000 (Invitrogen) for 6 h in immunoblotting experiments.
GPNMB reporter activity
The GPNMB reporter was generated by PCR amplification of a 530 bp promoter fragment upstream of the GPNMB gene using genomic DNA from LNCaP cells as a template. The following forward and reverse primers, containing terminal NcoI sites and Kozak sequence, were used for amplification: GPNMB_promoter_F:CATGccatggCCAACATAGTGAAACCTGCC; GPNMB_promoter_R:CATGccatggtggcTGAATTCTCACGGACGCAGG. Following amplification, the fragment was cloned into a Gateway-compatible entry vector upstream of a NanoLuc luciferase cassette using a unique NcoI site. The GPNMB-NanoLuc reporter was then transferred into a promoterless Gateway-compatible lentiviral destination vector carrying Blasticidin resistance for use in downstream studies. Lentiviral particles were prepared using HEK293T cells as a packaging line. HeLa and Hela-GFP-TFEB cells were transduced with viral particles and selected using 5ug/ml Blasticidin S to create stable lines. To measure the GPNMB promoter activity cells were transfected with appropriate siRNA constructs for 48 h and then seeded on opaque-white 96-well plates, allowed to attach and luminescence was assessed using Nano-Glo Luciferase assay system (Promega) on Biotek Synergy HT multi-well plate reader. All data were normalized to cell seeding.
mRNA extraction and real-time PCR
mRNA was isolated using the RNeasy Plus Micro Kit with on-column genomic DNA-digest (Qiagen) according to the manufacturer’s protocol. To generate cDNA from isolated and purified mRNA, Affinity Script quantitative PCR (qPCR) cDNA Synthesis Kit (Agilent Technologies) was used. Real-time PCR was conducted using StepOne Plus Realtime PCR Machine (Applied Biosystems) with TaqMan Real-Time PCR Master Mix (Thermo Fisher Scientific). Gene expression was measured relative to β-actin and delta delta Ct (ΔΔCt) method was used to calculate the fold change differences of the experimental groups compared to the control group. TaqMan real-time PCR assays (Thermo Fisher Scientific) used for RT-PCR are listed in Supplementary Table 1.
Hela-TFEB-GFP cells were plated onto 35 mm glass bottom dishes (Mattek Life Sciences) and grown in the appropriate conditions, images were captured using Olympus Fluoview FV10i confocal microscope at 60x magnification in live cells.
MEFs and HeLa-TFEB-GFP cells were plated onto 35 mm glass bottom dishes, fixed with 2% paraformaldehyde (PFA) for 15 min, permeabilized with 0.1% Triton X-100 for 5 min and washed three times with PBS. Cells were then blocked for 30 min in 1% BSA and incubated with primary antibodies, TFEB (1:200) (Cell Signaling Technology, 32361), TFE3 (1:200) (Millipore Sigma, HPA023881), GFP (1:1000) (abcam, ab13970), and HA-tag (1:1000) (Cell Signaling Technology, 3724S) in blocking buffer (1% BSA) for 1 h. Cells were then washed three times with PBS and stained with secondary antibody (1:1000 dilution) anti-Rabbit Alexa Fluor Red 568 (Life Technologies), in blocking buffer (1% BSA) and kept in the dark for 1 h. DAPI (4′, 6-diamidino-2-phenylindole) (Sigma-Aldrich) was used to visualize nuclei. Cells were washed again three times with PBS and mounted with VECTASHIELD Antifade Mounting Medium (Vector Laboratories). Confocal images in HeLa-TFEB-GFP cells were analyzed for percentage of nuclear localization in ImageJ, the background was removed from the channel corresponding to the protein of interest (TFEB-GFP) using the rolling ball radius method. The DAPI channel was used to generate nuclear mask of corresponding cells. The threshold of the DAPI image was adjusted, transformed to binary and then analyzed to produce nuclear regions of interest (ROI). This mask was then applied to the protein of interest (TFEB-GFP) channel and the total fluorescence intensity of the nuclear ROIs was measured. Phalloidin (1:1000) (Alexa Fluor 568 Phalloidin, A12380) channel was used to generate a mask of the whole cell of which an ROI was created and applied to the protein of interest channel to measure the total fluorescence intensity of the whole cell. The percentage of nuclear localization was calculated as described58. Three random images, each containing multiple cells, were used for quantification and statistical analysis in each condition.
Nuclear to cytoplasmic TFEB ratio in Fig. 5b, c was quantified using CellProfiler (https://cellprofiler.org/). DAPI staining was used to identify the nuclei and phalloidin staining (1:1000) was used to create a mask that defines the cytoplasmic compartment in each cell. The mean intensities of the nuclear and cytoplasmic regions were measured and used to calculate nuclear to cytoplasmic ratio.
Immunohistochemistry (IHC) was performed on formalin-fixed, paraffin-embedded, and sectioned kidneys from Tsc2+/− mice and human kidney and lung tissue samples. Human tissue samples were obtained with the approval of Partners Healthcare Human Research Committee. Briefly, slides were incubated at 65 C°, dewaxed with Xylene and ethanol and then rehydrated with H20. Antigen retrieval was performed in citrate buffer (ph = 6) by a heat-induced process using a Russell Hobbs pressure cooker, washed three times in H20, and then blocked with 5% goat serum in TBS for 1 h at room temperature. Sections were incubated overnight at 4 °C with primary antibodies: TFEB (1:150) (Cell Signaling Technology, 32361), NPC1 (1:400) (Abcam, ab134113), and HMB45 (1:100) (Dako, M0643) in blocking buffer. After washing three times in TBS, cells were incubated with ImmPRESS-HRP species-specific secondary antibodies (Vector Laboratories), washed again three times in TBS, and then incubated with ImmPACT DAB peroxidase substrate (Vector Laboratories, #SK4105). Finally, slides were counterstained with haematoxylin (Dako, #S3309), washed in tap water, dehydrated in ethanol and xylene prior to mounting with DPX (Sigma-Aldrich, #06522).
Immunohistochemistry image analysis
IHC slides were analyzed with ImageJ as described before59. In brief, the plug-in “color deconvolution” was executed on the digitalized IHC sections using the built-in vector HDAB, which separates the staining into 3 different panels with hematoxylin, DAB only image, and background. The DAB panel was used to measure the mean gray value of the region of interest. Then, the optical density (absorbance) was inferred by taking the log10 of the ratio of the maximum value of an 8-bit image (255) over the measured mean gray value60. To measure nuclear DAB staining, we first used the hematoxylin panel and adjusted the threshold in order to generate a nuclear mask. This mask was then analyzed to generate nuclear ROIs which were applied to the DAB panel and the optical density was calculated.
All quantitative, normally distributed data for in vitro studies were analyzed for statistical significance using a Student’s unpaired t-test, One-way ANOVA, and Tukey’s post hoc tests when comparing more than two groups relative to a single factor, or two-way ANOVA and Tukey’s post hoc tests when comparing more than two groups relative to more than one factor.
GraphPad Prism Software (GraphPad Prism version 8.3.1 for Windows; GraphPad Software, www.graphpad.com) was used.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
All data are available from the corresponding author upon request and all unique materials generated (such as HeLa TSC2 CRISPR KO line) are readily available from the authors. Source data for Figs. 1b, f, g, h, i, 2b, e, g, h, f, 3a–f, 4a–c, 5a, c–i, 6c and Suppl Figs. 1b, e, 4a–c, 5a, b, 6a–c, 7b, 8b, c are provided with this paper in a Source data file and a separate supplementary pdf file with uncropped western blots. Source data are provided with this paper.
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This work was supported by the Lucy Engles Fund for TSC and LAM Research (to E.P.H.), the Claudia Adams Program for Innovative Cancer Research (to S.R.V.), and a FAR grant from the Universita’ Politecnica Delle Marche (to M.M.). We thank Craig A. Strahdee for providing parental luciferase reporter plasmids.
The authors declare no competing interests.
Peer review information Nature Commnuications thanks the anonymous reviewers for their contributions to the peer review of this work. Peer review reports are available.
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Alesi, N., Akl, E.W., Khabibullin, D. et al. TSC2 regulates lysosome biogenesis via a non-canonical RAGC and TFEB-dependent mechanism. Nat Commun 12, 4245 (2021). https://doi.org/10.1038/s41467-021-24499-6