Table 1 Identification of an efficient strategy for HDR in Arabidopsis.

From: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis

Construct / Background Number of T1 plants analyzed HDR at the left HDR at the right HDR at both sides Overall HDR efficiency Precise HDR efficiency
EC-Cas9 / DD45 235 20 42 11 21.7% 4.68%
EC-Cas9 / DMC1 230 11 36 6 17.8% 2.61%
DMC1-Cas9 / DD45 210 10 43 6 22.4% 2.86%
DMC1-Cas9 / DMC1 224 4 18 0 9.8% 0%
  1. Two transgenic lines in which Cas9 is expressed under either the DD45 promoter (also called EC1.2 promoter, the egg cell-specific promoter 1.2) or the DISRUPTION OF MEIOTIC CONTROL 1 (DMC1) promoter were transformed with two gene drive constructs. One construct used a hybrid promoter, which contains the enhancer of EC1.2 and the EC1.1 promoter and which is referred to as EC here. The other construct used the DMC1 promoter to drive Cas9 expression. The Cas9 gene in the gene drive constructs was Zea mays codon-optimized. The Cas9 in the DD45-Cas9 line was the human codon-optimized version. The Cas9 in the DMC1-Cas9 background line was plant codon optimized. Overall HDR efficiency is calculated as the percent of plants in which at least one HDR event occurred.