Fig. 3: Biased inheritance mediated by a CRISPR/Cas9-based gene drive. | Nature Communications

Fig. 3: Biased inheritance mediated by a CRISPR/Cas9-based gene drive.

From: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis

Fig. 3

A plant with a wild type (WT) CRY1 copy in one chromosome and the gene drive element (red) inserted in CRY1 in the homologous chromosome underwent self-pollination to produce progeny. Several genotypes are expected: both copies of CRY1 are WT (CRY1/CRY1); one copy of CRY1 had NHEJ without disrupting CRY1 function such as a 3 bp deletion (cry1); one copy of CRY1 had NHEJ resulting in loss of CRY1 function (cry1*); heterozygous cry1; homozygous cry1; and plants with one copy of the gene drive insertion and one copy of cry1 that had undergone NHEJ repair (cry1/cry1*) that disrupted CRY1 function. The genotypes of CRY1/CRY1, CRY1/cry1, CRY1/cry1*, CRY1/cry1, and cry1/cry1 were not phenotypically different. Plants with either cry1/cry1 or cry1/cry1* genotypes display long hypocotyls in light conditions.

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