Fig. 1: Neuronal correlates of fear extinction in subpopulations of CEA neurons. | Nature Communications

Fig. 1: Neuronal correlates of fear extinction in subpopulations of CEA neurons.

From: Central amygdala micro-circuits mediate fear extinction

Fig. 1

a Behavioural protocol. FC: fear conditioning. CS: conditioned stimuli. b Behavioural data. B6 mice: n = 27; freezing, habituation, no CS: 19.8 ± 2.6%, CS: 26.1 ± 3.3%, beginning of extinction 1, no CS: 18.5 ± 2.3%, CS: 61.9 ± 4.6%, end of extinction 2, no CS: 25.3 ± 3.1%, CS: 34.1 ± 3.4%, blocks (averages) of 4 CSs. One-way repeated-measures ANOVA F(5,130) = 30.8, p < 0.001, followed by post hoc Bonferroni t-test vs. CS group during habituation, p < 0.001. Bar plots are expressed as means ± SEM. Circles are freezing values of individual mice. c Raster plots and corresponding spike waveforms of a representative CEm unit (top). Normalized and averaged population peri-stimulus time histograms (bottom). CEm neurons: n = 15 units from 5 mice; z-score habituation: −0.11 ± 0.45, beginning of extinction 1: 4.21 ± 1.75, end of extinction 2: 1.24 ± 0.48, blocks of 4 CSs. One-way repeated-measures ANOVA F(2,28) = 3.9, p = 0.033 followed by post hoc Bonferroni t-test vs. during habituation, p = 0.023. d Raster plots and corresponding spike waveforms of a representative CEloff unit (top). Normalized and averaged population peri-stimulus time histograms (bottom). CEloff neurons: n = 33 units from 18 mice; z-score, habituation: 0.28 ± 0.33, beginning of extinction 1: −1.53 ± 0.28, end of extinction 2: −0.46 ± 0.34, blocks of 4 CSs. One-way repeated-measures ANOVA F(2,64) = 8.4, p < 0.001 followed by post hoc Bonferroni t-test vs. during habituation, p < 0.001. e Raster plots and corresponding spike waveforms of a representative CElon unit (top). Normalized and averaged population peri-stimulus time histograms (bottom). CElon neurons: n = 55 units from 15 mice; z-score, habituation: 1.30 ± 0.30, beginning of extinction 1: 2.54 ± 0.43, end of extinction 2: 1.40 ± 0.30, blocks of 4 CSs. One-way repeated-measures ANOVA F(2,108) = 5.3, p = 0.006 followed by post hoc Bonferroni t-test vs. during habituation, p = 0.008. All individual neurons of each CEA population had significant z-score values upon CS presentation (4 first CSs during extinction 1). Source data are provided as a Source data file.

Back to article page