Fig. 5: Distinct chromatin accessibility patterns exist in neuroendocrine subpopulations. | Nature Communications

Fig. 5: Distinct chromatin accessibility patterns exist in neuroendocrine subpopulations.

From: Temporal evolution of cellular heterogeneity during the progression to advanced AR-negative prostate cancer

Fig. 5

a t-SNE plot of scATAC-seq data from PRN mice. b Chromatin accessibility in the subpopulations at the indicated loci. c Graphs of differential transcription factor motif enrichment in the indicated NE subpopulations called on regions of hyper-accessible and hypo-accessible chromatin between clusters. d Gene expression levels of the top 50 most differentially accessible genes in the NE1 and NE2 populations. Genes identified in Supplementary Table 5 and projected onto UMAP from Fig. 4g. e POU2F3 expression in benign (n = 29), locally advanced prostate cancer (PCa) (n = 66), CRPC (n = 73), and NEPC (n = 36) patient samples. f Summary of POU2F3 IHC staining on clinical tissue microarray. g Quantification of IHC scores in TMA by patient diagnosis (CRPC: n = 22; NEPC: n = 8). Box represents 25%−75% percentile with median denoted as a line. Whiskers extend +/−1.5× interquartile range. Data points that lie outside the whiskers are plotted individually. h Representative images of POU2F3-strong and POU2F3-negative/weak patient samples. Scale bar: 50 μm.

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