Fig. 4: Cell trajectories towards a neuroendocrine-like lineage. | Nature Communications

Fig. 4: Cell trajectories towards a neuroendocrine-like lineage.

From: Temporal evolution of cellular heterogeneity during the progression to advanced AR-negative prostate cancer

Fig. 4

a UMAP projection of luminal (red), basal (green), and neuroendocrine (blue) populations from PRN mice (n = 3). b Pseudotime trajectories projected onto UMAP from panel a. Color reflects pseudotime distance (neuroendocrine population located at t = 0). c Gene expression levels of indicated genes in individual cells over pseudotime in PRN (left) and PR (right) mice. Color reflects pseudotime distance (neuroendocrine population located at t = 0). d Heatmap and clustering of gene expression modules which vary significantly as a function of pseudotime in PRN mice. e GSEA results showing the top 5 gene sets enriched in modules 9, 19, and 20. f Expression of genes from modules 9, 19, and 20 projected onto PRN (left) and PR (right) mice. g Expression of indicated genes in luminal and neuroendocrine cells of 6-week-old (n = 2) and 8-week-old (n = 3) PRN mice. h UMAP of scRNA-seq data from a metastatic NEPC sample. Clusters were named based on GSEA results of differentially expressed genes between clusters.

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