Fig. 3: Single cell-based approaches reveal NEPC-like subpopulations. | Nature Communications

Fig. 3: Single cell-based approaches reveal NEPC-like subpopulations.

From: Temporal evolution of cellular heterogeneity during the progression to advanced AR-negative prostate cancer

Fig. 3

a H&E and IHC staining of AR and INSM1 in samples used for scRNA-seq. Scale bar: 50 μm. b The combined t-SNE plot of scRNA-seq data from intact PR (n = 3) and PRN (n = 3) mice. c Clustering of the top 50 most differentially expressed genes between clusters. d Reclustering of combined luminal, basal, and neuroendocrine populations from PR (n = 3) and PRN (n = 3) mice. e Distribution of genotypes between clusters identified in panel d. f Expression of indicated markers or signature in PR (n = 3) compared to PRN (n = 3) mice. Pie charts show proportions of positive vs. negative cells or high/medium/low/negative gene expression. g Violin plot of Smith ASC score25. h ASC score by cluster from panel d.

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