Fig. 5: mitoARCUS-induced increase in mt-tRNAAla in the liver. | Nature Communications

Fig. 5: mitoARCUS-induced increase in mt-tRNAAla in the liver.

From: Mitochondrial targeted meganuclease as a platform to eliminate mutant mtDNA in vivo

Fig. 5

a Northern blot analysis of juvenile mouse liver 24 weeks PI probed for mt-tRNAAla and total RNA loading (28S and 18S). b Quantification of mt-tRNAAla (from panel a) normalized to 28S rRNA (n = 3). Riboruler high-range molecular marker was used, but the lowest marker was 200 nt, whereas the tRNA are ~75 nt. c Quantification of mt-tRNAAla by qPCR compared to levels of mt-tRNAVal in juvenile mouse liver 24 weeks PI. RNA samples from AAV9-mitoARCUS-treated animals compared to AAV9-GFP controls and WT liver samples (n = 3 with the exception of GFP n = 4). d Quantification of mt-tRNAAla compared to levels of mt-tRNAVal in adult mouse liver. RNA samples from AAV9-mitoARCUS-treated animals compared to AAV9-GFP controls and WT liver samples, at 24 weeks PI (n = 4 with the exception of WT n = 3). Data are mean ± SEM. Statistical analysis was performed using two-tailed Student’s t-test.

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