Fig. 5: OVs can be used as adjuvants for vaccination against cancer neo-epitopes. | Nature Communications

Fig. 5: OVs can be used as adjuvants for vaccination against cancer neo-epitopes.

From: Adjuvant oncolytic virotherapy for personalized anti-cancer vaccination

Fig. 5

a Treatment schedule used in this study. IFNγ ELISPOT analyses of splenocytes from mice immunized with; b polyI:C co-administered with different B16F10Muts (IM) and re-stimulated ex-vivo with the same or the corresponding non-mutated peptides (n = 2 (WT restim) and 6 (Mut restim)); c polyI:C or MRB co-administered with different B16F10Muts and re-stimulated ex-vivo with the same peptides or (from left to right; n = 4, 4, 3 and 3); d polyI:C co-administered with 1, 2 or 4 different Muts (100 μg each) and re-stimulated ex-vivo with Mut30 (n = 5). Unless indicated otherwise, the statistical analyses refer to the comparison between the corresponding “No restim” and “restim” conditions. NS: p > 0.05, *: p < 0.05, **: p < 0.01, ***: p < 0.001 (unpaired two-tailed t-test). Tumor growth (left panels) and Kaplan–Meier survival analyses (right panels) of B16F10 SC tumor-bearing mice immunized with; e polyI:C ± Mut30 on days 7 and 14 or (n = 5), Data are presented as mean values ± SEM.; f polyI:C or MRB + Mut30 on day 7 (n = 10). Data are presented as mean values ± SEM. Tumor growth analyses: NS: p > 0.05, *: p < 0.05, ***: p < 0.001 (unpaired multiple two-tailed t-tests) and survival analyses: NS: p > 0.05, *: p < 0.05, **: p < 0.01, ***: p < 0.001 (Mantel–Cox test, two-sided). Source data are provided as a Source Data file. Exact p values can be found in the Source Data.

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