Fig. 4: OVs can function as both priming and boosting adjuvants in heterologous vaccination regimens. | Nature Communications

Fig. 4: OVs can function as both priming and boosting adjuvants in heterologous vaccination regimens.

From: Adjuvant oncolytic virotherapy for personalized anti-cancer vaccination

Fig. 4

a Treatment schedule used in this study. b IFNγ ELISPOT analysis of splenocytes from mice primed with Ad-DCT on day 7 and boosted with MRB-DCT on day 14 or primed and boosted with Ad and MRB co-administered with DCT peptide (left graph); or primed with Ad-Ova on day 7 and boosted with MRB-Ova on day 14 or primed and boosted with Ad and MRB co-administered with Ova peptide (right graph) (from left to right; n = 2, 5 and 5). The statistical analyses refer to the comparison between the corresponding ex-vivo “No restim” and “restim” conditions. NS: p > 0.05, ***: p < 0.001 (unpaired two-tailed t-test). Kaplan–Meier survival analyses of B16F10 lung tumor-bearing mice treated with; c Ad (day 7) and MRB (day 14) or Ad and MRB co-administered with DCT peptide (n = 10 (Ad, MRB and Ad+DCT, MRB + DCT) and 11 (NT)); d Ad-DCT on day 7 and MRB-DCT or MRB co-administered with DCT peptide on day 14 or (n = 7 (Ad-DCT, MRB-DCT and Ad+DCT, MRB + DCT) and 9 (NT)); e Ad-Ova on day 7 and MRB co-administered with Ova peptide on day 14 with or without CD8 depletion (n = 20 (NT and Ad+Ova, MRB + Ova) and 19 (Ad + Ova, MRB + Ova -CD8), 2 experiments combined). p > 0.05, *: p < 0.05, **: p < 0.01, ***: p < 0.001 (Mantel–Cox test, two-sided). Source data are provided as a Source Data file. Exact p values can be found in the Source Data.

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