Fig. 6: Gene expression profiling by RNA-seq of peptide-stimulated PBMC from Pandemrix-associated NT1 patients. | Nature Communications

Fig. 6: Gene expression profiling by RNA-seq of peptide-stimulated PBMC from Pandemrix-associated NT1 patients.

From: Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1

Fig. 6

Hierarchically clustered heatmap of the gene expression levels (z-score scaling generated with the Heatmapper program) in all tested PBMC samples for 74 genes showing significant differences in expression (**p < 0.01) between NT1 patients and controls either after stimulation with NA175–189 (49 genes) or POMT1675–689 peptides (28 genes; overlap of 3). Differentially expressed genes were identified using edgeR, based on a test analogous to Fisher’s exact test. The paired method was used for comparisons, calculating 2-sided p-values and adjusting for multiple testing using BH correction. If significance was reached (*p < 0.05), log2 fold changes between NT1 patients and controls are shown on the right. Data derived from FACS-sorted CD4+ and CD8+ cells available from one NT1 patient were added for comparison.

Back to article page