a Effect of TIM4 blockade on activation of endogenous CD8+ T cells in the blood of KP-OVA challenged mice. The percentage of IFN-γ+ CD8 and OVA specific-IFN-γ+ CD8 (Vα2/Vβ5+) as a function of total CD8 and Vα2/Vβ5+ T cells, respectively, at various time points after challenge. Isotype n = 5, αTIM4 n = 4, data represent one of two independent experiments. b–d Activation of anti-tumor T cell responses in WT and Timd4−/− animals 7 days after challenge with KP-OVA. b Representative plots and frequencies of IFN-γ+ endogenous CD8+ T cells in MLN. c Absolute numbers of CD8+ T cells and d OVA-specific CD8+ T cells (pentamers+) accumulated at tumor site. Data are representative of two experiments (n = 5 mice/group). e–g Lethally irradiated CD45.1 WT mice were injected with a 1:1 mixture of CD45.2 bone marrows of the indicated genotypes to generate bone marrow chimeras. Ten weeks later mice were inoculated with KP-OVA tumors. e Representative plots and frequencies of IFN-γ+ CD8+ T cells in MLN 10 days after tumor challenge (n = 6 mice/group). f Absolute numbers and quantification of CD8+ T cells accumulated at tumor site with Batf3−/−;WT n = 6 and Batf3−/−;Timd4−/− n = 5 mice. g Tumor growth in bone marrow chimeras was assessed 28 days after tumor challenge. Representative sections and quantification of tumor nodules calculated automatically on four consecutive sections of three lobes/animal and expressed as a function of the total lobe area. Each value in the plot represents the average of the three lobes and four sections for each mouse. All data are mean ± SEM. Significance was determined by two-way ANOVA followed by Sidak’s post-test for a, two-tailed unpaired t-test for b–f, and two-tailed Mann–Whitney test for g. Source data are provided as a Source Data file.