Fig. 2: Validation of MaxEnt model based on spatial location microscopy data. | Nature Communications

Fig. 2: Validation of MaxEnt model based on spatial location microscopy data.

From: Learning the distribution of single-cell chromosome conformations in bacteria reveals emergent order across genomic scales

Fig. 2

A Average scaled long-axis position predicted from MaxEnt models (solid lines) inferred from various Hi–C data sets (from26), including wild-type cells (black), rifampicin-treated cells (blue), and Δsmc cells (orange), together with results from microscopy experiments (adapted from17). Also shown are simulated data for a random polymer with ori-pole tether (dash-dotted gray line), and a simulated confined random polymer (dashed gray line), oriented such that ori is always on the left cell half. B The distribution of single-cell positions (scaled long-axis position) of chromosomal loci (blue: ori, red: pilA, green: pleC, orange: podJ), as predicted by the MaxEnt model (solid lines), together with previous experimental data from microscopy experiments (bars, adapted from17). To indicate experimental variability, the solid/transparent bars indicate the minimum/maximum measured by two different methods: FROS or FISH. To enable a direct comparison between model and experiment, the model values are distributed over the same number of bins as the experiment. The dotted lines indicate the distribution for a confined oriented random polymer as in A.

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