Fig. 4: Image data analysis reveals a particular hematopoietic and stromal composition for ILC niches. | Nature Communications

Fig. 4: Image data analysis reveals a particular hematopoietic and stromal composition for ILC niches.

From: Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells

Fig. 4

a In total, 10 µm radius around ILCs is defined as niches. Immune cells and components of the stromal compartment within these areas are quantified. Middle panel: cytotoxic T cells (Tc cells; magenta), T helper cells (Th cells; yellow), vessels (green), and fibronectin fibers (red) within one representative niche. Lower panel: fluorescence images using the same color code. Scale bar: 10 µm. b Box plot depicts absolute cell numbers within 74 ILC niches analyzed in five independent experiments. Analysis performed by one-way ANOVA (F = 3.921 and 4 df) with Bonferroni´s multiple comparisons test, where *p < 0.01. c Dot plot shows the distribution of cell types in all tissue areas compared to their distribution within ILC niches. Analysis performed by two-way ANOVA (F = 4.108 and 4 df) with Bonferroni´s multiple comparisons test, where *p < 0.05. d Box plot shows frequencies of ILCs localized within a 10 µm radius from vessels and fibronectin fibers. bd Data are shown as mean ±  SD. a, c, d (n = 5). Source data are provided as a Source data file.

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