Fig. 1: Panel overview of a 53 marker MELC run in the human tonsil. | Nature Communications

Fig. 1: Panel overview of a 53 marker MELC run in the human tonsil.

From: Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells

Fig. 1

Each image depicts the same field of view, sequentially stained with the depicted fluorescence-labeled antibodies, including surface proteins and transcription factors. The color code highlights markers used for lineage exclusion (magenta) and ILC inclusion (cyan) and markers shown in white represent exploratory markers for ILC and overall cell characterization. Images contain 2048 × 2048 pixels and are generated using an inverted wide-field fluorescence microscope with a 20× objective, a lateral resolution of 325 nm, and an axial resolution above 5 µm. Gnz A granzyme A, Fbn fibronectin, SMA smooth muscle actin, Vα7.2 TCR Vα7.2. Scale bar: 100 µm (n = 5).

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