Cell motilities of 8024-Vec/RALYL, MIHA-Vec/RALYL (a) and Huh7-Control/shRALYLs (b) were assessed by transwell migration and matrigel invasion assays. The number of migrated or invaded cells is shown in the bar chart. The values represent the mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, two-sided Student’s t-test). Scale bar = 200 μm. c Representative images of livers derived from nude mice after intrasplenic injection of indicated cells (up) and representative H&E staining of the corresponding liver sections (down). Scale bar = 200 μm. The number of metastatic nodules on the liver surface (indicated by yellow arrows) is summarized in the bar charts (right) and represent mean ± SD of four mice of PLC-8024-Vec, five mice of PLC-8024-RALYL, five mice of Huh7-Control or shRALYL, and four mice of H2M-Control or shRALYL (*P < 0.05, **P < 0.01, independent Student’s t test). d The protein expression level of E-cadherin, N-cadherin, fibronectin, SNAIL, and SLUG of cells transfected with Vec and RALYL, or Control and shRALYLs.