Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats

Kobayashi, Toshihiro; Goto, Teppei; Oikawa, Mami; Sanbo, Makoto; Yoshida, Fumika; Terada, Reiko; Niizeki, Naoko; Kajitani, Naoyo; Kazuki, Kanako; Kazuki, Yasuhiro; Hochi, Shinichi; Nakauchi, Hiromitsu; Surani, M. Azim; Hirabayashi, Masumi

doi:10.1038/s41467-021-21557-x

Download PDF

Article
Open access
Published: 26 February 2021

Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats

Nature Communications volume 12, Article number: 1328 (2021) Cite this article

7057 Accesses
26 Citations
117 Altmetric
Metrics details

Subjects

Abstract

Murine animal models from genetically modified pluripotent stem cells (PSCs) are essential for functional genomics and biomedical research, which require germline transmission for the establishment of colonies. However, the quality of PSCs, and donor-host cell competition in chimeras often present strong barriers for germline transmission. Here, we report efficient germline transmission of recalcitrant PSCs via blastocyst complementation, a method to compensate for missing tissues or organs in genetically modified animals via blastocyst injection of PSCs. We show that blastocysts from germline-deficient Prdm14 knockout rats provide a niche for the development of gametes originating entirely from the donor PSCs without any detriment to somatic development. We demonstrate the potential of this approach by creating PSC-derived Pax2/Pax8 double mutant anephric rats, and rescuing germline transmission of a PSC carrying a mouse artificial chromosome. Furthermore, we generate mouse PSC-derived functional spermatids in rats, which provides a proof-of-principle for the generation of xenogenic gametes in vivo. We believe this approach will become a useful system for generating PSC-derived germ cells in the future.

Anephrogenic phenotype induced by SALL1 gene knockout in pigs

Article Open access 29 May 2019

Generation of functional oocytes from male mice in vitro

Article 15 March 2023

Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD

Article 24 July 2019

Introduction

Genetically engineered animals provide essential insights into in vivo gene function and human genetic disorders. Pluripotent stem cells (PSCs) have been routinely used for targeted gene manipulation, particularly in rodents. PSCs can form all the tissues of the body, including the germline. Upon injection into the morula/blastocyst stage embryos, PSCs can carry their genetic modification to the next generation via germline transmission. While recent progress in genome editing technologies such as CRISPR/Cas9 in zygotes have enabled the generation of desired animals rapidly and efficiently^1,2, PSCs are useful for precise genetic modifications³. Introduction of point mutations or genes of interest via gene targeting and complex cell-based genetic modifications, such as microcell-mediated chromosome transfer, rely on PSCs^4,5. However, germline transmission of the genetic modifications by chimerism with PSCs can be variable and inefficient, partly because of the competition between the host and donor cells in chimeras that are affected by the quality of PSCs. An experimental approach that results in predictable functional gametes with desired genotypes from PSCs more efficiently is highly desirable. Here we apply the blastocyst complementation method to overcome the variable germline transmission of genomic modifications.

Blastocyst complementation can be used to generate a tissue or organ from donor PSCs in genetically modified animals. For example, injection of PSCs into blastocysts derived from animals that are unable to make certain tissues or organs allows donor PSCs to develop and compensates for the missing tissues or organs in chimeric animals. So far, other groups and we, have demonstrated the successful generation of several organs, such as the pancreas, thymus, kidney, liver, brain, and lung by blastocyst complementation^{6,7,8,9,10,11,12}. Such an approach is also possible in an interspecific setting between the mouse and rat^6,10,12. If the same strategy can be applied to the germline using germline-deficient animals, PSCs could develop efficiently as gametes even when the quality of PSCs is not optimum.

For a suitable host blastocyst lacking the germline, we focused on Prdm14, a key transcriptional regulator essential for the specification of primordial germ cells (PGCs), the founder cells for gametes. In rodents, a lack of Prdm14 causes loss of PGCs during development, which subsequently leads to infertility both in males and females. Importantly, the development of other organs and tissues is not impaired in these mutants^13,14. Therefore, Prdm14-deficient animal could serve as an appropriate host for the derivation of germ cells from donor PSCs.

Here, we demonstrate efficient germline transmission of PSCs via blastocyst complementation using germline-deficient Prdm14 knockout (KO) rats¹⁴. The rat is an important organism for physiological and pharmacological modeling of human diseases¹⁵. Achieving appropriate culture conditions to maintain germline-competent rat embryonic stem cells (ESCs) has enabled generation of genetically modified rat. ^16,17,18,19. However, rat PSCs are less robust than mouse PSCs, partly because of the difficulties of handling and clonal selection after genetic manipulation^20,21. Besides, rat PSCs exhibit rapid loss of genomic imprints in culture, which affects their developmental potential, as judged by tetraploid complementation experiments designed to produce an entirely PSC-derived animal^22,23. Here, we show that the use of rat blastocyst from Prdm14 KO rats serve as excellent hosts for the germline transmission of donor cells. We use our method to demonstrate the production of a double mutant rat line, and rescue of germline transmission of PSCs with a mouse artificial chromosome (MAC). Finally, we also show the derivation of viable mouse gametes using the Prdm14 KO rat host blastocysts.

Result

Blastocyst complementation using Prdm14 KO rats enables efficient germline transmission of allogenic embryonic stem cells (ESCs)

First, we tested if host Prdm14 KO rat embryos lacking germ cells permit the efficient contribution of exogenous rat PSCs to the germline. For this, we collected Prdm14 KO rat blastocysts, by crossing Prdm14^HV/+ with Prdm14^mut/+ rats (both with albino coat color: Fig. 1a). We selectively used Prdm14-H2BVenus positive blastocysts (Prdm14^HV/+ or Prdm14^mut/HV) to track Prdm14, since it is highly expressed in the inner cell mass of the rat blastocysts¹⁴. For donor rat PSCs, we used male and female rat ESCs (black coat color) ubiquitously expressing tdTomato established from homozygous Rosa26-tdTomato knock-in rats²⁴ (see “Methods”). After injecting ESCs into Prdm14-H2BVenus positive blastocysts, we transferred them into the uteri of foster mothers and analyzed their contribution towards the germline in the adults (Fig. 1b). In the Prdm14 heterozygous host (Prdm14^HV/+) where germ cells develop normally akin to wildtypes, rat ESCs contributed to somatic lineages and the germline depending on the extent of chimerism (Fig. 1c, d, Supplementary Table 1). After crossing with wild-type rats, a small proportion of offspring showed tdTomato fluorescence and dark skin (male: 16/174 [9.2%], female: 10/87 [11%], Fig. 1g, Supplementary Fig. 1a, Table 1, Supplementary Table 1), suggesting that germline transmission of donor rat ESCs occurred only in some neonatal rats. In contrast, in the Prdm14 KO host (Prdm14^mut/HV), rat ESCs contributed to somatic lineages like in the Prdm14 heterozygous rat, but all the germ cells in sex-matched chimeras showed tdTomato fluorescence (Fig. 1e, f, Supplementary Table 1). Whereas, the Prdm14 KO host has impaired PGC specification and development, and does not show any detectable germline contribution. Notably, all the next generation rats obtained after crossing with wild-type rats exhibited tdTomato fluorescence and dark skin (male: 85/85 [100%], female (31/31 [100%], Fig. 1h, Supplementary Fig. 1a, b, Table 1, Supplementary Table 1), suggesting complete germline transmission of donor rat ESCs without any host-derived germ cells. Collectively, we find that blastocyst complementation using host Prdm14 KO rat blastocysts enabled efficient germline transmission of donor rat ESCs.

**Fig. 1: Blastocyst complementation in the germline using *Prdm14* KO rat blastocysts and allogenic rat ESCs.**

Table 1 Blastocyst injection and germline transmission of ESCs.

Full size table

Efficient production of genetically modified rats using blastocyst complementation in the germline

Next, we investigated whether blastocyst complementation in the germline is a viable tool to generate genetically modified rats efficiently. To demonstrate this, we used Pax2/Pax8 double mutant rat ESCs as donor cells to obtain double KO rats. Both Pax2 and Pax8 are transcriptional regulators important for the proliferation and differentiation of multiple cell types, particularly in the kidney^25,26. Importantly, the Pax2/Pax8 double KO mice show a complete lack of kidneys as well as ureters. Sall1 KO animals are the closest anephric model for the Pax2/Pax8 double KO mice, lacking renal parenchyma; however, they retain ureters^7,10. Generating double KO’s using the conventional method, by crossing heterozygous mutants is highly inefficient with KO’s expected at a 1/16 Mendelian ratio. Thus, here we applied blastocyst complementation in the germline for efficient generation of Pax2/Pax8 double mutant rats.

We first screened male and female Rosa26-tdTomato rat ESC lines that have a frameshift mutation in Pax2 and Pax8 coding sequences created with the CRISPR/Cas9 system (Supplementary Fig. 2). Then, we injected them into Prdm14^HV/+ or Prdm14^mut/HV blastocysts (Fig. 2a). While donor ESCs lacking Pax2/Pax8, are expected not to form the kidney, the chimeras grew into normal adults without any impairment, suggesting host-derived cells developmentally rescued the defect. Indeed, the chimeras exhibited a lack of contribution of Pax2/Pax8 KO cells to renal lineages, including the ureters (Fig. 2b, Supplementary Fig. 2b–d). In contrast, all the germline in testes and ovaries of Prdm14 KO rats are composed of tdTomato expressing cells (Fig. 2b), confirming our previous result (Fig. 1), that the germline is complemented by donor Pax2/Pax8 KO rat ESCs. Next, we crossed male and female chimeras with Pax2/Pax8 KO gametes to obtain Pax2/Pax8 double KO rats. As expected, all the offspring lacked kidneys and ureters (Fig. 2c, Table 1). Thus, we could efficiently generate double KO rats using blastocyst complementation through the germline.

**Fig. 2: Generation of genetically modified rats using blastocyst complementation in the germline.**

To further demonstrate the potential of blastocyst complementation via the germline and compare with available methods to produce genetically modified animals, we next used a rat ESC line carrying a MAC. MAC is an exogenous chromosome capable of replicating and segregating as an independent chromosome²⁷. MAC is a useful tool for creating humanized model rodents that recapitulate human disorders. MACs inserts are several megabase long sequences, which cannot be achieved by conventional embryonic transgenesis approaches²⁸. Technically, the introduction of MAC requires cell-based transgenesis using PSCs or male germline stem cells^27,29. Thus, the successful production of the MAC carrying animals relies mainly on the quality of the cells. Here, we used male rat ESCs carrying a MAC containing EGFP expression cassette (Fig. 2a, d). When we injected the MAC rat ESCs into wild-type or Prdm14 heterozygous rat blastocysts, we were unable to obtain any chimeras showing signs of germline contribution of the MAC rat ESCs in the testes, despite testing over 50 sex-matched chimeras (Table 1). While most of them showed EGFP fluorescence in the testis (Fig. 2e), only the somatic cells surrounding the germ cells were EGFP-positive in the sections (Fig. 2f), indicating that the MAC rat ESC line we used in this study shows almost no germline contribution. In contrast, in chimeras obtained from Prdm14 KO rat blastocysts, we could observe seminiferous tubules filled with EGFP-positive cells in the adult testes (Fig. 2e). The tubules contained male germ cells at all stages of development, including mature spermatozoa (Fig. 2f). To obtain the next generation carrying the MAC, we collected and sorted EGFP-positive round spermatids from the chimeric testes (Fig. 2g). Since expression of Prdm14-H2BVenus is absent in adult testes of both Prdm14^HV/+ and Prdm14^mut/HV³⁰, we can selectively sort donor MAC rat ESC-derived cells from the adult testis. Next, we performed a round spermatid injection (ROSI) into oocytes from wild-type female rats. After embryo transfer (ET), we successfully obtained 21 pups, and 8 of them showed ubiquitous expression of EGFP (Fig. 2h, Table 2). All the adults show the black eye and coat color, corresponding to the original ESCs (Supplementary Figure 1d). Notably, EGFP-positive adult shows a presence of the MAC in all the cells in the brain (Supplementary Fig. 1c), suggesting that round spermatid cells generated using Prdm14 KO blastocysts carry the MAC to the next generation.

Table 2 Injection of ESC-derived round spermatids into oocytes.

Full size table

Generation of functional mouse spermatids in rat via interspecific blastocyst complementation in the germline

Next, we investigated whether blastocyst complementation in the germline applies to an interspecific setting between the mouse and rat. To monitor specification and subsequent development of mouse PGCs in rat, we used mouse ESCs established from the Blimp1-membrane (m) EGFP reporter transgenic mouse (Blimp1^{Tg(BAC-mEGFP)/+}), a faithful line to monitor PGCs until gonadal stage³¹. The EGFP protein in Blimp1 expressing mouse PGCs localizes at the membrane. Whereas, the Venus signal in Prdm14-H2BVenus rat PGCs is nuclear. Therefore the mouse PGCs are readily distinguishable from rat PGCs. In addition, for visualizing the contribution to somatic cells as well as the germline after blastocyst injection, we introduced the CAG promoter-driven mScarlet-3xNLS (nmScarlet) expressing vector via a PiggyBac system into mouse ESCs (Supplementary Figure 3a). Upon injection of the labeled mouse ESC line into mouse blastocysts, we confirmed their competence towards forming chimeras and in germline transmission (Supplementary Fig. 3b–h). We injected the mouse ESCs into Prdm14-H2BVenus positive rat blastocysts and confirmed contribution to the germline during development (Fig. 3a). At 6 days after ET corresponding to embryonic day (E) 9–9.5 when rat PGCs specify, Prdm14 KO PGCs expressing H2BVenus were detected in the posterior epiblast (dashed line in Fig. 3b), although the rat PGCs show reduced proliferation and fail to activate key PGC markers due to the lack of Prdm14³². Strikingly however, Blimp1-mEGFP/CAG-nmScarlet positive cells were also observed in the PGC cluster, suggesting that the rat environment is conducive for mouse ESC-derived PGC specification (Fig. 3b). At 12 days post ET (E15-15.5), mouse PGCs were undetectable in Prdm14 heterozygous rat gonads, although nmScarlet positive somatic cells surrounding rat PGCs were present (Supplementary Fig. 4b). In contrast, Blimp1-mEGFP/CAG-nmScarlet positive mouse PGCs expressing DAZL, a key gonadal PGC marker, colonized Prdm14 KO rat gonads normally devoid of rat PGCs (Fig. 3c, Supplementary Fig. 4b). The somatic contribution of mouse ESCs in rats is, however, not as high as in mice (Supplementary Fig. 4c). In the adult chimeras with Prdm14 KO, two out of three testes showed nmScarlet expression within the seminiferous tubules, and strikingly, normal spermatogenesis could be observed in the section (Fig. 3d, e, Table 1). In the epididymis, we could also observe mature sperm with mScarlet signal in the nucleus (Fig. 3f), but they somehow rarely show motility after being harvested into human tubal fluid (HTF) medium suitable for mouse sperm³³. Similarly, hamster sperm also showed abnormalities when transplanted into mouse testes³⁴, suggesting that spermiogenesis might be impaired in xenogeneic environments; the precise reasons merits further considerations. To test the function of mouse ESC-derived male germ cells generated in rats, we performed ROSI of the FACS-sorted mouse ESC-derived round spermatid into oocytes collected from a wild-type mouse. We successfully obtained healthy mouse offspring, and all of them showed ubiquitous nmScarlet expression, and almost half of them inherited Blimp1-mEGFP transgene at a Mendelian ratio (7/20 [35.0%], Fig. 3g, Table 2)

**Fig. 3: Interspecific blastocyst complementation in germline generates functional mouse spermatids in rats.**

Finally, we also tested mouse induced pluripotent stem cells (iPSCs) from CAG-EGFP transgenic mouse^6,35,36 as donor cells for blastocyst complementation in the germline (Table 1). As with mouse ESCs, EGFP-positive mouse iPSC-derived spermatogenesis was observed in Prdm14^mut/HV rat testes (Fig.3h, i). Notably, the haploid cells were able to produce viable mice after injection into mouse oocytes (Fig. 3j). Almost half of the mice expressed EGFP ubiquitously (16/37 [43%], Fig. 3j, Table 2), and all the pups checked here inherited retroviral integrations of a reprograming factor used for the establishment of iPSCs (Fig. 3k). Taken together, we demonstrated that interspecific blastocyst complementation in the germline can yield functional mouse spermatids in rats.

Discussion

We demonstrate applications of blastocyst complementation in the germline using Prdm14 KO rat embryos as hosts, with allogenic rat ESCs as well as xenogenic mouse ESCs as donor cells. Since our approach allows efficient germline transmission, we were able to produce rat lines bearing elaborate, as well as precise genetic modifications. Lacking competition from host germ cells unlike in conventional chimeras, our method enriches for germ cells with desired genotypes.

By using the blastocyst complementation method, we demonstrated the efficient generation of Pax2/Pax8 double mutant rats lacking both renal parenchyma and ureters. For blastocyst complementation in the kidney, we previously used blastocysts from Sall1 KO mice or rats^7,10. However, since Sall1 KO animals show defects in the renal mesenchyme but not in the ureteric bud during renal development^10,37, the ureters in the chimera consist of both injected donor PSCs and host-derived cells. Such contamination of host-derived tissues might prevent efficient engraftment of PSC-derived kidneys in transplantation therapy. Thus, for complete complementation of organs, host lines with multiple mutations will prove beneficial in the future. Blastocyst complementation in the germline is an effective way to generate such elaborate genetically modified animals.

Successful germline transmission is a stringent criterion for determining PSC pluripotency³⁰. While mechanisms behind the success or failure of germline transmission are not fully known, the genetic background of the donor or host cells, chromosomal abnormalities, or PSC sources such as those from in vitro reprogramming are known to influence the germline potential^38,39. In our study, a rat ESC line carrying a MAC contributed normally to somatic tissues but not to the germline in wild-type or heterozygous animals where the host germ cells are present. This is likely due to the clone we used since previous reports have demonstrated successful germline transmission of PSCs with similar artificial chromosome vectors^27,28. Even when retaining a normal karyotype, not all rat PSC lines show germline transmission; indeed, line-to-line and clone-to-clone variations are common^21,40. Specifically, the complete lack of the host germ cells in Prdm14 KO rat however allowed rescue of the MAC rat ESCs, which formed functional gametes, demonstrating that the MAC rat ESC line itself can develop as germ cells. Yet, in the presence of host germ cells, the MAC bearing ESC-exhibit a disadvantage since germ cells from them are competitively eliminated from the germline. Therefore, the use of germline-deficient animals, might also test the competency of PSCs for germline transmission in the absence of competition from host germ cells.

Interspecific blastocyst complementation using Prdm14 KO rat with mouse ESCs or iPSCs can generate functional mouse male germ cells in rats. While rat PSCs can contribute to germline even in wild-type mouse^12,41,42, we hardly find mouse PGCs in rat gonads in the presence of the host rat PGCs (Supplementary Fig. 4b). Interestingly, at the onset of PGC specification and prior to their migration toward gonads, a significant number of mouse PGCs clustered with host rat PGCs in most of the Prdm14^HV/+ rat chimeras (Supplementary Fig. 4a). Thus, these chimeras with a high contribution of xenogenic cells might be eliminated due to the abnormality often seen in the interspecific chimeras^6,36, or due to competition between mouse and rat PGCs, particularly in the gonad where they proliferate actively. In any case, mouse PSCs do not readily contribute to the germline in rats compared with rat PSCs in the mouse. Previously, we demonstrated that the contribution of xenogenic PSCs in interspecific chimeras between mouse and rat varies greatly from organ to organ³⁶. For instance, in the kidney, rat PSCs contribute poorly to the metanephric mesenchyme, the origin of renal parenchyma during embryonic development, leading to failure in the generation of rat kidney in Sall1 KO mouse⁷. Conversely, mouse kidneys are generated in the Sall1 KO rat owing to the efficient contribution of mouse PSCs to the metanephric mesenchyme¹⁰. Thus, the combination of the species in chimera creation dictates the success of contribution to the desired tissue or organ. Contribution to tissues such as the neuron or the germline also requires careful consideration due to ethical concerns when blastocyst complementation is applied to human organ regeneration in livestock⁴³. It is likely that such contribution might be low or negligible, depending on the particular species used as host.

Interspecific chimeras between the mouse and rat are generally smaller than intra-specific chimeras^6,36. Thus, the greater evolutionary distance between the donor and host species may limit the extent of chimerism, which will affect the contribution to the germline. However, the use of recipient embryos incapable of generating a host germline, such as in Prdm14 KO rats, increases the likelihood of xenogenic germ cells even with PSCs that contribute poorly to somatic tissues. Since in vitro reprogramming technology enables PSCs derivation from endangered species^44,45, the generation of xenogenic germ cells in germline-deficient animals could provide a useful approach to generate functional gametes for the preservation of such species.

Taken together, blastocyst complementation in the germline shown is a robust system for efficient generation of desired animals and xenogenic germ cells from PSCs.

Methods

Animals

Crlj:WI (RGD ID: 2312504) rats were purchased from Charles River Laboratories Japan, Inc. (Kanagawa, Japan). Slc:SD (RGD ID: 12910483) rats, C57BL/6NCrSlc, B6D2F1/Slc, and Slc:ICR mice were purchased from SLC Japan (Shizuoka, Japan). All experiments were performed in accordance with the animal care and use committee guidelines of the National Institute for Physiological Sciences.

Culture of PSC lines

Undifferentiated rat ESCs　(WDB-Rosa26em1(RT2)Nips-ES2/Nips, WDB/Nips-ES1/Nips, RGD ID: 10054010) were maintained on mitomycin-C-treated mouse embryonic fibroblasts in N2B27 medium (all components were purchased from Thermo Fisher Scientific, MA) containing 1 μM PD0325901 (Axon, Groeningen, The Netherlands), 3 μM CHIR99021 (Axon), and 1000 U/ml of rat leukemia inhibitory factor (Millipore, Bedford, MA). A female Rosa26^{tdTomato/tdTomato} rat ESC line used in Figs. 1 and 2 was established from blastocysts obtained from an intercross of Rosa26^{tdTomato/tdTomato} rats (WDB-ROSA26^em1(RT2)Nips) according to published protocol⁴⁶.

For making Pax2/Pax8 double KO rat ESCs, pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene plasmid # 42230) vectors expressing Cas9 protein with gRNA target for coding sequence of Pax2 and Pax8 were introduced by reverse transfection method using lipofectamine 2000 (Thermo Fisher Scientific) as described before⁴⁷. After transfection, we carried out limiting dilution to enrich single cell-derived colonies and screened for mutants by PCR and subsequent Sanger sequencing using the following primer sets: Pax2 (Fw, 5′- CCTCCATTTTTCAGCTCTGC-3′, and Rv, 5′-CCCTCACTCCAAGTCTCAGC-3′), Pax8 (Fw, 5′-TCCCCAAATGGTGTGTTTCT-3′, and Rv, 5′- CGTTGGTGTTCCTTGTCCTT-3′).

For making rat ESCs carrying MAC, MAC was transferred to rat ESCs via microcell-mediated chromosome transfer²⁸. Briefly, rat ESCs (BLK2i-1, RGD ID: 10054010) were fused with microcells prepared from the donor hybrid cells, and selected with G418 (150 µg/mL). CHO cells containing a MAC with human chromosome 21 region, which was developed previously⁴⁸, was used as donor hybrid cells in this study.

Undifferentiated mouse ESCs (established from Blimp1^{Tg(BAC-mEGFP)/+} mouse in previous reports^49,50) and mouse iPSCs (GT3.2 line⁶) were maintained on 0.1% gelatin-coated plates in Ndiff227 medium (Takara Bio, Shiga, Japan) containing 1 μM PD0325901, 3 μM CHIR99021, 1,000 U/ml of mouse leukemia inhibitory factor (Millipore), and 5% fetal calf serum (FCS, batch tested for mouse ESCs; Thermo Fisher Scientific). To monitor the ESC-derived cells in chimeras, a PiggyBac vector containing CAG-driven mScarlet with 3x nuclear localization signals cassettes were transfected into mouse ESCs with PBase plasmid by reverse transfection method. The transfected mouse ESCs were seeded on gelatin-coated plates and 48 h later, 0.8 μg/ml puromycin (Sigma-Aldrich, MO) was added to the culture medium for selection. Puromycin-resistant and nmScarlet positive mouse ESC colonies were picked and used for the experiment.

Genotyping

To detect transgenes in germline transmitted pups or adults, genomic DNA was analyzed by PCR using the following primer sets: tdTomato (Fw, 5′-GCGAGGAGGTCATCAAAGAG-3′, and Rv, 5′-GATGACGGCCATGTTGTTGT-3′), CAG-nmScarlet (Fw, 5′-GCCTCTGCTAACCATGTTCA-3′, and Rv, 5′-CTTGTACAGCTCGTCCATGCCG-3′), Blimp1-mEGFP (Fw, 5′-TAAACGGCCACAAGTTCAGCG-3′, and Rv, 5′-CTCCTTGAAGTCGATGCCCTT-3′), CAG-EGFP (Fw, 5′-GCCTCTGCTAACCATGTTCA-3′, and Rv, 5′-CTCCTTGAAGTCGATGCCCTT-3′), pMXs-Oct3/4 (Fw, 5′-CCCTGGGGATGCTGTGAGCCAAGG-3′, and Rv, 5′-CCCTTTTTCTGGAGACTAAATAAA-3′).

FISH analyses

The trypsinized rat ESCs and homogenized brain samples were incubated for 15 min in 0.075 M KCl, fixed with methanol and acetic acid (3:1), and then slides were prepared using standard methods. FISH analyses were performed using fixed metaphase spreads of each cell hybrid using digoxigenin-labeled (Roche, Basel, Switzerland) DNA [mouse COT-1 DNA] and biotin-labeled DNA [human COT-1 DNA (Invitrogen)]⁵¹. Chromosomal DNA was counterstained with DAPI (Sigma-Aldrich). Images were captured using an AxioImagerZ2 fluorescence microscope (Carl Zeiss GmbH).

Embryo manipulation

A step-by-step protocol describing blastocyst complementation can be found at our protocol published previously⁵².

For rat blastocyst injection, blastocysts were collected in mR1ECM medium from the oviduct and uterus of rats at 4.5 days post coitum (dpc). For micro-manipulation, rat or mouse ESCs were trypsinized and suspended in the ESC medium, respectively. A piezo-driven micro-manipulator (Prime Tech, Tokyo, Japan) was used to drill into the zona pellucida and trophectoderm under the microscope and 7–8 rat ESCs were introduced into blastocyst cavities near the inner cell mass. After the injection, the blastocysts were transferred into the uterus of pseudopregnant recipient Crlj:WI rats (3.5 dpc).

For mouse blastocyst injection, morula stage embryos were collected in KSOM medium from oviduct and uterus of mice at 2.5 dpc, and then cultured for 1 day until they reached the blastocyst stage. Mouse ESCs were trypsinized and suspended in the ESC medium. As in the rat blastocyst injection, 7–8 mouse ESCs were introduced into blastocyst cavities near the inner cell mass. After injection, the blastocysts were transferred into the uterus of pseudopregnant recipient Slc:ICR mice (2.5 dpc).

For rat ROSI, seminiferous tubules were dissociated mechanically and EGFP-positive MAC rat ESC-derived rat round spermatids were collected with the SH800 cell sorter (SONY) according to a published protocol⁵³. Sorted round spermatids were suspended in PBS containing glucose, sodium lactate, and FCS and used as donor cells. Metaphase II stage oocytes were collected in HEPES-KRB medium from superovulated Slc:SD rats and denuded the cumulus with 0.1% hyaluronidase treatment. Rat oocytes were then pretreated with 5 mM ionomycin for 5 min and incubated for 40 min in mKRB medium until injection. After ROSI, the fertilized embryos were treated for 4 h with 5 µg/mL cycloheximide (Sigma-Aldrich) in mKRB. After follow-up incubation in mKRB for 1 day, embryos were transferred into the oviduct of pseudo-pregnant Crlj:WI rats.

For mouse ROSI, nmScarlet positive mouse ESC-derived mouse round spermatids were collected with the SH800 cell sorter and used as donor cells. Metaphase II stage oocytes were collected in CZB medium from B6D2F1/Slc mice and denuded the cumulus with 0.1% hyaluronidase treatment. Before ROSI, mouse oocytes were treated with 10 mM SrCl₂ for 20 min. After follow-up incubation of the mouse embryos in CZB for 1 day, embryos were transferred into the oviduct of pseudo-pregnant Slc:ICR mice.

Histological analysis

Tissues were fixed in 4% PFA for 4 h to O/N at RT depending on the size. Whole embryos and embryonic gonads were fixed in 4% PFA for 30 min at RT or for 6 h at 4 °C. For cryosections, the fixed embryos were embedded in OCT compound. Each sample was incubated with primary antibodies for 1–2 h at RT or O/N at RT or 4 °C, and then with fluorescent-conjugated secondary antibodies with DAPI (FUJIFILM Wako, Osaka, Japan) for 1 h at RT or O/N at 4 °C. Antibodies used here are listed in Supplementary Table 2. The embryos and cryosections were observed under confocal laser scanning microscopy (FV3000; Olympus, Tokyo, Japan). For imaging, we carefully performed all the experiments with appropriate controls. To judge positive fluorescent signals, we obtained the images alongside samples of chimera or non-chimera that have partial or no contribution of donor cells.

Progeny testing to confirm germline transmission of Rosa26 ^{tdTomato/tdTomato} rat ESCs

Chimeric rats generated by injection of male or female Rosa26^{tdTomato/tdTomato} rat ESCs were crossed with Crlj:WI (RGD ID: 2312504) rats. Since ESCs are homozygous for Rosa26-tdTomato, all the pups with germline transmission ubiquitously express tdTomato fluorescence in all the tissues. In addition, since rat ESCs originate from WDB/Nips black rat (RGD ID: 7411634), the resulting pups from germline transmission of the rat ESCs also clearly show black coat color and black eye by the effect of the dominant gene. Thus, we judged the number of pups resulting from the germline transmission of tdTomato fluorescence, and the coat and eye color appearance.

Blood chimerism analysis

Peripheral blood samples obtained from each chimera were hemolyed by red blood cell lysis buffer. After washing the cells with PBS containing 3% FCS, the percentage of donor PSC-derived cells were determined by fluorescent signals in the blood cells detected by SH800 cell sorter.

Data reproducibility

IF images in Figs. 1d, f, 2b, f, 3b, c, e, i, Supplementary Figs. 2b,d, 3b,c, 4a,b were representatives of multiple sections at different projections (6<). Karyotyping images in Fig. 2d and Supplementary Fig. 1c were representatives obtained from multiple cells on each slide. Image in Fig. 3f is representative of two animals. Genotyping in Fig. 3k, Supplementary Figs. 1a and 3h was performed one time with appropriate loading controls.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

The authors declare that all data supporting the findings of this study are available within the article and its supplementary information files or from the corresponding author upon reasonable request. Source data are provided with this paper.

References

Barrangou, R. & Doudna, J. A. Applications of CRISPR technologies in research and beyond. Nat. Biotechnol. 34, 933–941 (2016).
Article CAS PubMed Google Scholar
Wang, H. et al. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153, 910–918 (2013).
Article CAS PubMed PubMed Central Google Scholar
Oji, A. et al. CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice. Sci. Rep. 6, 31666 (2016).
Article ADS CAS PubMed PubMed Central Google Scholar
Doherty, A. M. O. & Fisher, E. M. C. Microcell-mediated chromosome transfer (MMCT): small cells with huge potential. Mamm. Genome 14, 583–592 (2003).
Article PubMed Google Scholar
Uno, N., Abe, S., Oshimura, M. & Kazuki, Y. Combinations of chromosome transfer and genome editing for the development of cell/animal models of human disease and humanized animal models. J. Hum. Genet. 63, 145–156 (2018).
Article CAS PubMed Google Scholar
Kobayashi, T. et al. Generation of rat pancreas in mouse by interspecific blastocyst injection of pluripotent stem cells. Cell 142, 787–799 (2010).
Article CAS PubMed Google Scholar
Usui, J. et al. Generation of kidney from pluripotent stem cells via blastocyst complementation. Am. J. Pathol. 180, 2417–2426 (2012).
Article CAS PubMed Google Scholar
Chang, A. N. et al. Neural blastocyst complementation enables mouse forebrain organogenesis. Nature 563, 126–130 (2018).
Article ADS CAS PubMed PubMed Central Google Scholar
Espejel, S. et al. Induced pluripotent stem cell–derived hepatocytes have the functional and proliferative capabilities needed for liver regeneration in mice. J. Clin. Investig. 120, 3120–3126 (2010).
Article CAS PubMed PubMed Central Google Scholar
Goto, T. et al. Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats. Nat. Commun. 10, 451 (2019).
Article ADS PubMed PubMed Central CAS Google Scholar
Mori, M. et al. Generation of functional lungs via conditional blastocyst complementation using pluripotent stem cells. Nat. Med. 25, 1691–1698 (2019).
Article CAS PubMed PubMed Central Google Scholar
Isotani, A., Hatayama, H., Kaseda, K., Ikawa, M. & Okabe, M. Formation of a thymus from rat ES cells in xenogeneic nude mouse<–>rat ES chimeras. Genes Cells 16, 397–405 (2011).
Article CAS PubMed Google Scholar
Yamaji, M. et al. Critical function of Prdm14 for the establishment of the germ cell lineage in mice. Nat. Genet, 40, 1016–1022 (2008).
Article CAS Google Scholar
Kobayashi, T. et al. Germline development in rat revealed by visualization and deletion of Prdm14. Development 147, dev183798 (2020).
Aitman, T. J. et al. Progress and prospects in rat genetics: a community view. Nat. Genet. 40, 516–522 (2008).
Article CAS PubMed Google Scholar
Buehr, M. et al. Capture of authentic embryonic stem cells from rat blastocysts. Cell 135, 1287–1298 (2008).
Article CAS PubMed Google Scholar
Li, P. et al. Germline competent embryonic stem cells derived from rat blastocysts. Cell 135, 1299–1310 (2008).
Article CAS PubMed PubMed Central Google Scholar
Tong, C., Li, P., Wu, N. L., Yan, Y. & Ying, Q. L. Production of p53 gene knockout rats by homologous recombination in embryonic stem cells. Nature 467, 211–213 (2010).
Article ADS CAS PubMed PubMed Central Google Scholar
Hirabayashi, M. et al. Rat transgenesis via embryonic stem cells electroporated with the Kusabira-orange gene. Mol. Reprod. Dev. 77, 474 (2010).
Article CAS PubMed Google Scholar
Blair, K., Wray, J. & Smith, A. The liberation of embryonic stem cells. PLoS Genet. 7, e1002019 (2011).
Article CAS PubMed PubMed Central Google Scholar
Blair, K. et al. Culture parameters for stable expansion, genetic modification and germline transmission of rat pluripotent stem cells. Biol. Open 1, 58–65 (2012).
Article CAS PubMed Google Scholar
Hirabayashi, M. et al. Ability of tetraploid rat blastocysts to support fetal development after complementation with embryonic stem cells. Mol. Reprod. Dev. 79, 402–412 (2012).
Article CAS PubMed Google Scholar
Li, T. D. et al. Rat embryonic stem cells produce fertile offspring through tetraploid complementation. Proc. Natl Acad. Sci. USA 114, 11974–11979 (2017).
Article CAS PubMed PubMed Central Google Scholar
Kobayashi, T. et al. Identification of rat Rosa26 locus enables generation of knock-in rat lines ubiquitously expressing tdTomato. Stem Cells Dev. 21, 2981–2986 (2012).
Article CAS PubMed PubMed Central Google Scholar
Narlis, M., Grote, D., Gaitan, Y., Boualia, S. K. & Bouchard, M. Pax2 and pax8 regulate branching morphogenesis and nephron differentiation in the developing kidney. J. Am. Soc. Nephrol. 18, 1121–1129 (2007).
Article CAS PubMed Google Scholar
Bouchard, M., Souabni, A., Mandler, M., Neubuser, A. & Busslinger, M. Nephric lineage specification by Pax2 and Pax8. Genes Dev. 16, 2958–2970 (2002).
Article CAS PubMed PubMed Central Google Scholar
Kazuki, K. et al. Highly stable maintenance of a mouse artificial chromosome in human cells and mice. Biochem. Biophys. Res. Commun. 442, 44–50 (2013).
Article CAS PubMed Google Scholar
Kazuki, Y. et al. Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism. Proc. Natl Acad. Sci. USA 116, 3072–3081 (2019).
Article CAS PubMed PubMed Central Google Scholar
Shinohara, T. et al. Transfer of a mouse artificial chromosome into spermatogonial stem cells generates transchromosomic mice. Stem Cell Rep. 9, 1180–1191 (2017).
Article CAS Google Scholar
De Los Angeles, A. et al. Hallmarks of pluripotency. Nature 525, 469–478 (2015).
Article ADS PubMed CAS Google Scholar
Ohinata, Y. et al. Blimp1 is a critical determinant of the germ cell lineage in mice. Nature 436, 207–213 (2005).
Article ADS CAS PubMed Google Scholar
Kobayashi, T. et al. Germline development in rat revealed by visualization and deletion of Prdm14. Development 147, dev183798 (2020).
Article CAS PubMed Google Scholar
Quinn, P., Kerin, J. F. & Warnes, G. M. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil. Steril. 44, 493–498 (1985).
Article CAS PubMed Google Scholar
Ogawa, T., Dobrinski, I., Avarbock, M. R. & Brinster, R. L. Xenogeneic spermatogenesis following transplantation of hamster germ cells to mouse testes1. Biol. Reprod. 60, 515–521 (1999).
Article CAS PubMed Google Scholar
Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T. & Nishimune, Y. ‘Green mice’ as a source of ubiquitous green cells. FEBS Lett. 407, 313–319 (1997).
Article CAS PubMed Google Scholar
Yamaguchi, T. et al. An interspecies barrier to tetraploid complementation and chimera formation. Sci. Rep. 8, 15289 (2018).
Article ADS PubMed PubMed Central CAS Google Scholar
Nishinakamura, R. et al. Murine homolog of SALL1 is essential for ureteric bud invasion in kidney development. Development 128, 3105–3115 (2001).
CAS PubMed Google Scholar
Carstea, A. C., Pirity, M. K. & Dinnyes, A. Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background. World J. Stem Cells 1, 22–29 (2009).
Article PubMed PubMed Central Google Scholar
Han, J. et al. Tbx3 improves the germ-line competency of induced pluripotent stem cells. Nature 463, 1096–1100 (2010).
Article ADS CAS PubMed PubMed Central Google Scholar
Hirabayashi, M. et al. A retrospective analysis of germline competence in rat embryonic stem cell lines. Transgenic Res. 22, 411–416 (2013).
Article CAS PubMed Google Scholar
Hamanaka, S. et al. Generation of germline-competent rat induced pluripotent stem cells. PLoS ONE 6, e22008 (2011).
Article ADS CAS PubMed PubMed Central Google Scholar
Isotani, A., Yamagata, K., Okabe, M. & Ikawa, M. Generation of Hprt-disrupted rat through mouse<-rat ES chimeras. Sci. Rep. 6, 24215 (2016).
Article ADS CAS PubMed PubMed Central Google Scholar
Rashid, T., Kobayashi, T. & Nakauchi, H. Revisiting the flight of Icarus: making human organs from PSCs with large animal chimeras. Cell Stem Cell 15, 406–409 (2014).
Article CAS PubMed Google Scholar
Honda, A. et al. Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis. Sci. Adv. 3, e1602179 (2017).
Article ADS PubMed PubMed Central CAS Google Scholar
Friedrich Ben-Nun, I. et al. Induced pluripotent stem cells from highly endangered species. Nat. Methods 8, 829–831 (2011).
Article CAS Google Scholar
Hirabayashi, M., Takizawa, A. & Hochi, S. Embryonic stem cells and gene manipulation in rat. Methods Mol. Biol. 2018, 115–130 (2019).
Article CAS PubMed Google Scholar
Kobayashi, T. et al. Principles of early human development and germ cell program from conserved model systems. Nature 546, 416–420 (2017).
Article ADS CAS PubMed PubMed Central Google Scholar
Kazuki, Y. et al. Refined human artificial chromosome vectors for gene therapy and animal transgenesis. Gene Ther. 18, 384–393 (2011).
Article CAS PubMed Google Scholar
Murakami, K. et al. NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers. Nature 529, 403–407 (2016).
Article ADS CAS PubMed PubMed Central Google Scholar
Hackett, J. A., Kobayashi, T., Dietmann, S. & Surani, M. A. Activation of lineage regulators and transposable elements across a pluripotent spectrum. Stem Cell Rep. 8, 1645–1658 (2017).
Article CAS Google Scholar
Tomizuka, K. et al. Functional expression and germline transmission of a human chromosome fragment in chimaeric mice. Nat. Genet. 16, 133–143 (1997).
Article CAS PubMed Google Scholar
Hirabayashi, M. & Hochi, S. Organ generation from knockedout rat blastocysts complemented with pluripotent stem cells. Methods Mol. Biol. 1874, 313–326 (2019).
Article CAS PubMed Google Scholar
Hayama, T. et al. Practical selection methods for rat and mouse round spermatids without DNA staining by flow cytometric cell sorting. Mol. Reprod. Dev. 83, 488–496 (2016).
Article CAS PubMed Google Scholar

Download references

Acknowledgements

We thank members of the Hirabayashi lab, in particular, Keiko Yamauchi and Megumi Hashimoto for help with animals, and Minako Ohnishi for secretarial support. We also thank Dr. Roopsha Sengupta for editing and providing critical input to the manuscript. This work was supported by grants from Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (18H02367) to M.H. and T.K., (18H05548) and (18H05544) to T.K., JST CREST (JPMJCR18S4) to Y.K., and LEAP-AMED (JP18gm0010002) to H.N. and M.H., AMED (JP18bm0704022) to T.K., and The Great Britain Sasakawa Foundation, Butterfield Awards in Medicine and Health (B115) to T.K. and M.A.S.

Author information

These authors contributed equally: Toshihiro Kobayashi, Teppei Goto, Mami Oikawa.

Authors and Affiliations

Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi, Japan
Toshihiro Kobayashi, Teppei Goto, Mami Oikawa, Makoto Sanbo, Fumika Yoshida, Reiko Terada, Naoko Niizeki & Masumi Hirabayashi
The Graduate University of Advanced Studies, Okazaki, Aichi, Japan
Toshihiro Kobayashi & Masumi Hirabayashi
Chromosome Engineering Research Center, Tottori University, Yonago, Tottori, Japan
Naoyo Kajitani, Kanako Kazuki & Yasuhiro Kazuki
Division of Genome and Cellular Functions, Department of Molecular and Cellular Biology, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Tottori, Japan
Yasuhiro Kazuki
Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan
Shinichi Hochi
Division of Stem Cell Therapy, Distinguished Professor Unit, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan
Hiromitsu Nakauchi
Institute for Stem Cell Biology and Regenerative Medicine, Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA
Hiromitsu Nakauchi
Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
M. Azim Surani
Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK
M. Azim Surani

Authors

Toshihiro Kobayashi
View author publications
You can also search for this author in PubMed Google Scholar
Teppei Goto
View author publications
You can also search for this author in PubMed Google Scholar
Mami Oikawa
View author publications
You can also search for this author in PubMed Google Scholar
Makoto Sanbo
View author publications
You can also search for this author in PubMed Google Scholar
Fumika Yoshida
View author publications
You can also search for this author in PubMed Google Scholar
Reiko Terada
View author publications
You can also search for this author in PubMed Google Scholar
Naoko Niizeki
View author publications
You can also search for this author in PubMed Google Scholar
Naoyo Kajitani
View author publications
You can also search for this author in PubMed Google Scholar
Kanako Kazuki
View author publications
You can also search for this author in PubMed Google Scholar
Yasuhiro Kazuki
View author publications
You can also search for this author in PubMed Google Scholar
Shinichi Hochi
View author publications
You can also search for this author in PubMed Google Scholar
Hiromitsu Nakauchi
View author publications
You can also search for this author in PubMed Google Scholar
M. Azim Surani
View author publications
You can also search for this author in PubMed Google Scholar
Masumi Hirabayashi
View author publications
You can also search for this author in PubMed Google Scholar

Contributions

T.K., T.G., M.O., and M.H. designed the study. T.K. wrote the manuscript with help of M.A.S. T.K., M.O., N.N., M.S., and M.H. performed embryo manipulation. T.K., T.G., M.O., and M.H. analyzed the chimeras. T.K., T.G., F.Y., and R.T. performed molecular biological analysis and cell culture. N.K., K.K., and Y.K. performed chromosome transfer and FISH analysis. M.A.S. provided materials. H.N. and S.H. supervised the project.

Corresponding authors

Correspondence to Toshihiro Kobayashi or Masumi Hirabayashi.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Peer review information Nature Communications thanks the anonymous reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Peer Review File

Reporting Summary

Source data

Source Data

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Cite this article

Kobayashi, T., Goto, T., Oikawa, M. et al. Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats. Nat Commun 12, 1328 (2021). https://doi.org/10.1038/s41467-021-21557-x

Download citation

Received: 22 May 2020
Accepted: 29 January 2021
Published: 26 February 2021
DOI: https://doi.org/10.1038/s41467-021-21557-x

This article is cited by

Replacing renal function using bioengineered tissues
- Melissa H. Little
- Ton J. Rabelink
Nature Reviews Bioengineering (2023)
Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice
- Manabu Ozawa
- Jumpei Taguchi
- Masahito Ikawa
Scientific Reports (2022)
Single-cell roadmap of human gonadal development
- Luz Garcia-Alonso
- Valentina Lorenzi
- Roser Vento-Tormo
Nature (2022)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.