Fig. 6: ENDU-2 prevents misexpression of soma-specific genes in the germline. | Nature Communications

Fig. 6: ENDU-2 prevents misexpression of soma-specific genes in the germline.

From: The secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans

Fig. 6

a Volcano plot of gonadal expressed genes in endu-2(tm4977) relative to wild type day-one adult animals grown up at 25 °C. Two biological replicates were obtained for each strain. Significantly misregulated genes (FC > 2, FDR < 0.05) were calculated with DESeq2 and are shown in red or blue. n refers to the total numbers of up- or downregulated genes. b Comparison of the total transcripts co-immunoprecipitated with ENDU-2 and ENDU-2 regulated genes in the gonad. c Comparison of expression levels (RPKM) of total gonad-expressed genes (n = 8356), ENDU-2 class I (n = 218) and class II targets (n = 11) in wild type animals. Shown are boxes extending from the 25th to the 75th percentile, with the median indicated by the horizontal line and Min/Max whiskers. Statistical test with one-way ANOVA. P-values were calculated with Dunnett’s multiple comparison test. P-value for total vs. class II targets: 0.5283. ***P-value <0.0001. d Comparison of relative expression between the soma and the gonad (SEF) of the total transcripts (n = 12371), ENDU-2 class I (n = 218), and class II (n = 11) targets in wild type animals. Shown are boxes extending from the 25th to the 75th percentile, with the median indicated by the horizontal line and Min/Max whiskers. Statistical test with one-way ANOVA. P-values were calculated with Dunnett’s multiple comparison test. P-value for total vs. class II targets: 0.1477. ***P-value <0.0001. e The majority of the alerted expression of the germline class I targets is rescued by endu-2(E454Q)::EGFP transgene. f Heat map of ENDU-2 repressed germline genes in wild type, endu-2(tm4977) and endu-2;Ex[endu-2(E454Q)::EGFP] animals.

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