a endu-2(wt)::EGFP rescues the Mrt phenotype at 25 °C. Left panel shows the experimental strategy for all rescue experiments with endu-2 transgenes in this study. endu-2(−/−) daughter generation (G1) that has lost the extrachromosomal rescue constructs expressing endu-2::EGFP was isolated. This and the following endu-2(−/−) generations (G2 to Gn) were compared to endu-2;Ex[endu-2:.EGFP] derived from the same P0 animal. Data are mean ± SD, N = 3. b Expression of endu-2(wt)::EGFP from neurons or intestine is sufficient to rescue the Mrt phenotype at 25 °C. Data are mean ± SD, N = 3. c SSsel-1::Δssendu-2::EGFP but not Δssendu-2::EGFP transgene rescues the Mrt phenotype at 25 °C. Data are mean ± SD, N = 3. d ENDU-2 primarily affects oocyte to maintain germline immortality. Data are pooled data from three biological replicates with similar tendencies in results.